Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,730,559	G→A	P491P (CCG→CCA)	lhr →	putative ATP‑dependent helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,730,559	0	G	A	100.0%	139.0 / NA	37	P491P (CCG→CCA)	lhr	putative ATP‑dependent helicase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/21); total (16/21)

TGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCCTCTCGACGTCCTTGCGCAGCAAACCGTTGCCGCCGCGGCGATGGATGCATTACAGGTAGACGAATGGTACTCCCGCGTACGCCGTGCCGCACCGTGGAAAGATCTGCCAAGACGTGTTTTTGACGCCACGCTGGATATGCTTTCCGGGCGCTATCCCTCTGGCGATTTTTCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGC  >  NC_000913/1730412‑1730697

tGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCCTCTCGACGTCCTTGCGCAGCAAACCGTTGCCGCCGCGGCGATGGATGCATTACAGGTAGACGAATGGTACTCCCGCGTACGCCGTGCCGCACCATgg                                                                                                                                         >  1:159138/1‑151 (MQ=255)

 gTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCCTCTCGACGTCCTTGCGCAGCAAACCGTTGCCGCCGCGGCGATGGATGCATTACAGGTAGACGAATGGTACTCCCGCGTACGCCGTGCCGCACCATgg                                                                                                                                         >  2:3728/1‑150 (MQ=255)

  tAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCCTCTCGACGTCCTTGCGCAGCAAACCGTTGCCGCCGCGGCGATGGATGCATTACAGGTAGACGAATGGTACTCCCGCGTACGCCGTGCCGCACCATGGa                                                                                                                                        >  2:139392/1‑150 (MQ=255)

   agagTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCCTCTCGACGTCCTTGCGCAGCAAACCGTTGCCGCCGCGGCGATGGATGCATTACAGGTAGACGAATGGTACTCCCGCGTACGCCGTGCCGCACCATGGaa                                                                                                                                       <  1:420013/150‑1 (MQ=255)

          aTGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCCTCTCGACGTCCTTGCGCAGCAAACCGTTGCCGCCGCGGCGATGGATGCATTACAGGTAGACGAATGGTACTCCCGCGTACGCCGTGCCGCACCATGGAAAGATCTg                                                                                                                                <  1:297450/150‑1 (MQ=255)

            gTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCCTCTCGACGTCCTTGCGCAGCAAACCGTTGCCGCCGCGGCGATGGATGCATTACAGGTAGACGAATGGTACTCCCGCGTACGCCGTGCCGCACCATGGAAAGATCTGcc                                                                                                                              >  2:561193/1‑150 (MQ=255)

                     caggcTGGAAAACCTGACACCACCGCATAATCCTCTCGACGTCCTTGCGCAGCAAACCGTTGCCGCCGCGGCGATGGATGCATTACAGGTAGACGAATGGTACTCCCGCGTACGCCGTGCCGCACCATGGAAAGATCTGCCAAGACGTGtt                                                                                                                    >  1:8469/1‑151 (MQ=255)

                      aggcTGGAAAACCTGACACCACCGCATAATCCTCTCGACGTCCTTGCGCAGCAAACCGTTGCCGCCGCGGCGATGGATGCATTACAGGTAGACGAATGGTACTCCCGCGTACGCCGTGCCGCACCATGGAAAGATCTGCCAAGACGTGttt                                                                                                                   >  1:653161/1‑151 (MQ=255)

                           ggAAAACCTGACACCACCGCATAATCCTCTCGACGTCCTTGCGCAGCAAACCGTTGCCGCCGCGGCGATGGATGCATTACAGGTAGACGAATGGTACTCCCGCGTACGCCGTGCCGCACCATGGAAAGATCTGCCAAGACGTGTTTTTGAc                                                                                                              >  2:592119/1‑151 (MQ=255)

                                 ccTGACACCACCGCATAATCCTCTCGACGTCCTTGCGCAGCAAACCGTTGCCGCCGCGGCGATGGATGCATTACAGGTAGACGAATGGTACTCCCGCGTACGCCGTGCCGCACCATGGAAAGATCTGCCAAGACGTGTTTTTGACGCCACg                                                                                                        >  2:69844/1‑151 (MQ=255)

                                 ccTGACACCACCGCATAATCCTCTCGACGTCCTTGCGCAGCAAACCGTTGCCGCCGCGGCGATGGATGCATTACAGGTAGACGAATGGTACTCCCGCGTACGCCGTGCCGCACCATGGAAAGATCTGCCAAGACGTGTTTTTGACGCCACg                                                                                                        <  1:179862/151‑1 (MQ=255)

                                     acacCACCGCATAATCCTCTCGACGTCCTTGCGCAGCAAACCGTTGCCGCCGCGGCGATGGATGCATTACAGGTAGACGAATGGTACTCCCGCGTACGCCGTGCCGCACCATGGAAAGATCTGCCAAGACGTGTTTTTGACGCCACGCTgg                                                                                                    <  2:349191/151‑1 (MQ=255)

                                     acacCACCGCATAATCCTCTCGACGTCCTTGCGCAGCAAACCGTTGCCGCCGCGGCGATGGATGCATTACAGGTAGACGAATGGTACTCCCGCGTACGCCGTGCCGCACCATGGAAAGATCTGCCAAGACGTGTTTTTGACGCCACGCTgg                                                                                                    <  2:15561/151‑1 (MQ=255)

                                      caccacCGCATAATCCTCTCGACGTCCTTGCGCAGCAAACCGTTGCCGCCGCGGCGATGGATGCATTACAGGTAGACGAATGGTACTCCCGCGTACGCCGTGCCGCACCATGGAAAGATCTGCCAAGACGTGTTTTTGACGCCACGCTgg                                                                                                    >  1:282009/1‑150 (MQ=255)

                                      caccacCGCATAATCCTCTCGACGTCCTTGCGCAGCAAACCGTTGCCGCCGCGGCGATGGATGCATTACAGGTAGACGAATGGTACTCCCGCGTACGCCGTGCCGCACCATGGAAAGATCTGCCAAGACGTGTTTTTGACGCCACGCTgg                                                                                                    >  2:433191/1‑150 (MQ=255)

                                      caccacCGCATAATCCTCTCGACGTCCTTGCGCAGCAAACCGTTGCCGCCGCGGCGATGGATGCATTACAGGTAGACGAATGGTACTCCCGCGTACGCCGTGCCGCACCATGGAAAGATCTGCCAAGACGTGTTTTTGACGCCACGCTGGa                                                                                                   <  2:8468/151‑1 (MQ=255)

                                       accaccGCATAATCCTCTCGACGTCCTTGCGCAGCAAACCGTTGCCGCCGCGGCGATGGATGCATTACAGGTAGACGAATGGTACTCCCGCGTACGCCGTGCCGCACCATGGAAAGATCTGCCAAGACGTGTTTTTGACGCCACGCTGGat                                                                                                  >  1:143804/1‑151 (MQ=255)

                                        ccaccGCATAATCCTCTCGACGTCCTTGCGCAGCAAACCGTTGCCGCCGCGGCGATGGATGCATTACAGGTAGACGAATGGTACTCCCGCGTACGCCGTGCCGCACCATGGAAAGATCTGCCAAGACGTGTTTTTGACGCCACGCTGGat                                                                                                  >  1:415424/1‑150 (MQ=255)

                                           ccGCATAATCCTCTCGACGTCCTTGCGCAGCAAACCGTTGCCGCCGCGGCGATGGATGCATTACAGGTAGACGAATGGTACTCCCGCGTACGCCGTGCCGCACCATGGAAAGATCTGCCAAGACGTGTTTTTGACGCCACGCTGGATATGc                                                                                              <  1:155126/151‑1 (MQ=255)

                                                tAATCCTCTCGACGTCCTTGCGCAGCAAACCGTTGCCGCCGCGGCGATGGATGCATTACAGGTAGACGAATGGTACTCCCGCGTACGCCGTGCCGCACCATGGAAAGATCTGCCAAGACGTGTTTTTGACGCCACGCTGGATATGCTTTcc                                                                                         >  2:208603/1‑151 (MQ=255)

                                                         cGACGTCCTTGCGCAGCAAACCGTTGCCGCCGCGGCGATGGATGCATTACAGGTAGACGAATGGTACTCCCGCGTACGCCGTGCCGCACCATGGAAAGATCTGCCAAGACGTGTTTTTGACGCCACGCTGGATATGCTTTCCGGGCGCTAt                                                                                <  2:74239/151‑1 (MQ=255)

                                                          gACGTCCTTGCGCAGCAAACCGTTGCCGCCGCGGCGATGGATGCATTACAGGTAGACGAATGGTACTCCCGCGTACGCCGTGCCGCACCATGGAAAGATCTGCCAAGACGTGTTTTTGACGCCACGCTGGATATGCTTTCCGGGCGCTATc                                                                               <  2:134633/151‑1 (MQ=255)

                                                            cGTCCTTGCGCAGCAAACCGTTGCCGCCGCGGCGATGGATGCATTACAGGTAGACGAATGGTACTCCCGCGTACGCCGTGCCGCACCATGGAAAGATCTGCCAAGACGTGTTTTTGACGCCACGCTGGATATGCTTTCCGGGCGCTATccc                                                                             <  2:159141/151‑1 (MQ=255)

                                                                  tGCGCAGCAAACCGTTGCCGCCGCGGCGATGGATGCATTACAGGTAGACGAATGGTACTCCCGCGTACGCCGTGCCGCACCATGGAAAGATCTGCCAAGACGTGTTTTTGACGCCACGCTGGATATGCTTTCCGGGCGCTATCCCTCTGGc                                                                       >  1:24550/1‑151 (MQ=255)

                                                                            aCCGTTGCCGCCGCGGCGATGGATGCATTACAGGTAGACGAATGGTACTCCCGCGTACGCCGTGCCGCACCATGGAAAGATCTGCCAAGACGTGTTTTTGACGCCACGCTGGATATGCTTTCCGGGCGCTATCCCTCTGGCGATTTTTCTg                                                             <  1:427513/151‑1 (MQ=255)

                                                                              cGTTGCCGCCGCGGCGATGGATGCATTACAGGTAGACGAATGGTACTCCCGCGTACGCCGTGCCGCACCATGGAAAGATCTGCCAAGACGTGTTTTTGACGCCACGCTGGATATGCTTTCCGGGCGCTATCCCTCTGGCGATTTTTCTGCt                                                           <  1:401384/151‑1 (MQ=255)

                                                                                             gatggatgCATTACAGGTAGACGAATGGTACTCCCGCGTACGCCGTGCCGCACCATGGAAAGATCTGCCAAGACGTGTTTTTGACGCCACGCTGGATATGCTTTCCGGGCGCTATCCCTCTGGCGATTTTTCTGCTTTTCGCCCCAAACTg                                            >  1:614035/1‑151 (MQ=255)

                                                                                                      aTTACAGGTAGACGAATGGTACTCCCGCGTACGCCGTGCCGCACCATGGAAAGATCTGCCAAGACGTGTTTTTGACGCCACGCTGGATATGCTTTCCGGGCGCTATCCCTCTGGCGATTTTTCTGCTTTTCGCCCCAAACTGGTCTGGAAc                                   <  2:620248/151‑1 (MQ=255)

                                                                                                         aCAGGTAGACGAATGGTAGTCCCGCGTACGCCGTGCCGCACCATGGAAAGATCTGCCACGACGTGTTTTTGACGCCACGCTGGATATGCTTTCCGGGCGCTATCCCTCTGGCGATTTTTCTGCTTTTCGCCCCAAACTGGTCTGGAACa                                  <  1:288143/149‑1 (MQ=255)

                                                                                                         aCAGGTAGACGAATGGTACTCCCGCGTACGCCGTGCCGCACCATGGAAAGATCTGCCAAGACGTGTTTTTGACGCCACGCTGGATATGCTTTCCGGGCGCTATCCCTCTGGCGATTTTTCTGCTTTTCGCCCCAAACTGGTCTGGAACAg                                 <  2:338084/150‑1 (MQ=255)

                                                                                                                     aTGGTACTCCCGCGTACGCCGTGCCGCACCATGGAAAGATCTGCCAAGACGTGTTTTTGACGCCACGCTGGATATGCTTTCCGGGCGCTATCCCTCTGGCGATTTTTCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGata                    <  1:380308/151‑1 (MQ=255)

                                                                                                                       ggTACTCCCGCGTACGCCGTGCCGCACCATGGAAAGATCTGCCAAGACGTGTTTTTGACGCCACGCTGGATATGCTTTCCGGGCGCTATCCCTCTGGCGATTTTTCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATAtt                  <  2:506220/151‑1 (MQ=255)

                                                                                                                        gTACTCCCGCGTACGCCGTGCCGCACCATGGAAAGATCTGCCAAGACGTGTTTTTGACGCCACGCTGGATATGCTTTCCGGGCGCTATCCCTCTGGCGATTTTTCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTg                 <  2:39959/151‑1 (MQ=255)

                                                                                                                              ccGCGTACGCCGTGCCGCACCATGGAAAGATCTGCCAAGACGTGTTTTTGACGCCACGCTGGATATGCTTTCCGGGCGCTATCCCTCTGGCGATTTTTCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGgaga                            >  2:410001/1‑134 (MQ=255)

                                                                                                                              ccGCGTACGCCGTGCCGCACCATGGAAAGACCTGCCAAGACGTGTTTTTGACGCCTCGCTGGATATGCTTTCCGGGCGCTATCCCTCTGGCGATTTTTCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGgaga                            <  1:409996/134‑1 (MQ=255)

                                                                                                                                    aCGCCGTGCCGCACCATGGAAAGATCTGCCAAGACGTGTTTTTGACGCCACGCTGGATATGCTTTCCGGGCGCTATCCCTCTGGCGATTTTTCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCt     <  2:199621/151‑1 (MQ=255)

                                                                                                                                       ccGTGCCGCACCATGGAAAGATCTGCCAAGACGTGTTTTTGACGCCACGCTGGATATGCTTTCCGGGCGCTATCCCTCTGGCGATTTTTCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGgc  <  1:480375/151‑1 (MQ=255)

TGTAGAGTGTATGTTCGCAGGCAGGCTGGAAAACCTGACACCACCGCATAATCCTCTCGACGTCCTTGCGCAGCAAACCGTTGCCGCCGCGGCGATGGATGCATTACAGGTAGACGAATGGTACTCCCGCGTACGCCGTGCCGCACCGTGGAAAGATCTGCCAAGACGTGTTTTTGACGCCACGCTGGATATGCTTTCCGGGCGCTATCCCTCTGGCGATTTTTCTGCTTTTCGCCCCAAACTGGTCTGGAACAGGGAGACCGGGATATTGACCGCCCGACCTGGC  >  NC_000913/1730412‑1730697

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A