Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,832,946	T→C	R173R (CGT→CGC)	xthA →	exonuclease III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,832,946	0	T	C	100.0%	131.7 / NA	37	R173R (CGT→CGC)	xthA	exonuclease III
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/16); total (21/16)

AAAAGCGCAGTTTTATCAGAATCTGCAAAACTACCTGGAAACCGAACTCAAACGTGATAATCCGGTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTGGGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAG  >  NC_000913/1832799‑1833082

aaaaGCGCAGTTTTATCAGAATCTGCAAAACTACCTGGAAACCGAACTCAAACGTGATAATCCGGTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGCAcc                                                                                                                                       >  2:542228/1‑151 (MQ=255)

          ttttATCAGAATCTGCAAAACTACCTGGAAACCGAACTCAAACGTGATAATCCGGTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGCACCGGTAAATGct                                                                                                                             >  2:59270/1‑151 (MQ=255)

           tttATCAGAATCTGCAAAACTACCTGGAAACCGAACTCAAACGTGATAATCCGGTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGCACCGGTAAATGctc                                                                                                                            >  1:260017/1‑151 (MQ=255)

            ttATCAGAATCTGCAAAACTACCTGGAAACCGAACTCAAACGTGATAATCCGGTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGCACCGGTAAATGctc                                                                                                                            <  2:277834/150‑1 (MQ=255)

                cAGAATCTGCAAAACTACCTGGAAACCGAACTCAAACGTGATAATCCGGTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGCACCGGTAAATGCTCTTTcc                                                                                                                       <  1:579914/151‑1 (MQ=255)

                   aagcTGCAAAACTACCTGGAAACCGAACTCAAACGTGATAATCCGGTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGCACCGGTAAATGCTCTTTCCTGc                                                                                                                    <  1:360106/148‑1 (MQ=255)

                        gCAAAACTACCTGGAAACCGAACTCAAACGTGATAATCCGGTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGCACCGGTAAATGCTCTTTCCTGCCgg                                                                                                                 <  2:630107/149‑1 (MQ=255)

                                   tGGAAACCGAACTCAAACGTGATAATCCGGTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGCACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAatg                                                                                                    >  1:584466/1‑151 (MQ=255)

                                               tCAAACGTGATAATCCGGTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGCACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAgg                                                                                          >  2:590753/1‑149 (MQ=255)

                                                  aaCGTGATAATCCGGTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGCACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCtgat                                                                                     >  1:246172/1‑151 (MQ=255)

                                                    cGTGATAATCCGGTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGCACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCtgatga                                                                                   <  2:117079/151‑1 (MQ=255)

                                                      tGATAATCCGGTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGCACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAg                                                                                  >  2:392273/1‑150 (MQ=255)

                                                             ccGGTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGCACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTGGGGGt                                                                          <  2:14941/151‑1 (MQ=255)

                                                              cGGTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGCACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTGGGGGtt                                                                         >  1:10832/1‑151 (MQ=255)

                                                               ggTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGCACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTGGGGGTTg                                                                        >  2:283/1‑151 (MQ=255)

                                                                  aCTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGCACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTGGGGGTTGGt                                                                      <  1:369741/150‑1 (MQ=255)

                                                                  aCTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGCACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTGGGGGTTGGTc                                                                     >  2:44006/1‑151 (MQ=255)

                                                                    tGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGCACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTGGGGGTTGGTCg                                                                    >  2:119692/1‑150 (MQ=255)

                                                                         aTGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGCACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTGGGGGTTGGTCGATACCt                                                              >  2:97586/1‑151 (MQ=255)

                                                                         aTGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGCACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTGGGGGTTGGTCGATACCt                                                              >  1:300424/1‑151 (MQ=255)

                                                                                    gAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGCACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTGGGGGTTGGTCGATACCTTCCGCCATGCg                                                   <  2:285249/151‑1 (MQ=255)

                                                                                          cAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGCACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTGGGGGTTGGTCGATACCTTCCGCCATGCGAATcc                                              <  2:345200/150‑1 (MQ=255)

                                                                                            gCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGCACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTGGGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCa                                           <  2:381465/151‑1 (MQ=255)

                                                                                               cTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGCACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTGGGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAAc                                        >  1:276601/1‑151 (MQ=255)

                                                                                                 aCAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGCACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTGGGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAAca                                       <  1:7419/150‑1 (MQ=255)

                                                                                                   aGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGCACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATAGACAGGCTGATGAGCTGGGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAAcagc                                     >  1:515796/1‑150 (MQ=255)

                                                                                                          gATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGCCCCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTGGGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGAt                                 <  1:328631/147‑1 (MQ=255)

                                                                                                             atCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGCACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTGGGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTtc                           >  1:406485/1‑150 (MQ=255)

                                                                                                                        cGAAGAAAACCGTAAGCGCTGGCTGCGCACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTGGGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGAt               >  2:490469/1‑151 (MQ=255)

                                                                                                                         gaagaaAACCGTAAGCGCTGGCTGCGCACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTGGGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGAtt              >  1:272533/1‑151 (MQ=255)

                                                                                                                             aaaaCCGTAAGCGCTGGCTGCGCACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTGGGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCg          >  1:177048/1‑151 (MQ=255)

                                                                                                                              aaaCCGTAAGCGCTGGCTGCGCACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTGGGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGc         <  2:194968/151‑1 (MQ=255)

                                                                                                                              aaaCCGTAAGCGCTGGCTGCGCACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTGGGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGc         >  2:216018/1‑151 (MQ=255)

                                                                                                                                 ccGTAAGCGCTGGCTGCGCACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTGGGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTc       <  1:60425/150‑1 (MQ=255)

                                                                                                                                   gTAAGCGCTGGCTGCGCACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTGGGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCaa     >  1:500513/1‑150 (MQ=255)

                                                                                                                                     aaGCGCTGGCTGCGCACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTGGGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAg  <  2:330724/151‑1 (MQ=255)

                                                                                                                                     aaGCGCTGGCTGCGCACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTGGGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAg  <  2:72972/151‑1 (MQ=255)

AAAAGCGCAGTTTTATCAGAATCTGCAAAACTACCTGGAAACCGAACTCAAACGTGATAATCCGGTACTGATTATGGGCGATATGAATATCAGCCCTACAGATCTGGATATCGGCATTGGCGAAGAAAACCGTAAGCGCTGGCTGCGTACCGGTAAATGCTCTTTCCTGCCGGAAGAGCGCGAATGGATGGACAGGCTGATGAGCTGGGGGTTGGTCGATACCTTCCGCCATGCGAATCCGCAAACAGCAGATCGTTTCTCATGGTTTGATTACCGCTCAAAAG  >  NC_000913/1832799‑1833082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A