Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F50 I2 R1	104	44.1	1376607	98.9%	1361464	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	657,830	T→C	T37A (ACG→GCG)	flc ←	fluoride efflux channel, dual topology membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	657,830	0	T	C	100.0%	92.1 / NA	26	T37A (ACG→GCG)	flc	fluoride efflux channel, dual topology membrane protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (14/12); total (14/12)

GCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTG  >  NC_000913/657685‑657970

gCCGAAAATGTTGAGAGGTTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCcccc                                                                                                                                          <  1:684007/150‑1 (MQ=255)

                  gttgttAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGc                                                                                                                        >  1:612041/1‑150 (MQ=255)

                    tgttAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGt                                                                                                                     >  2:490879/1‑151 (MQ=255)

                      ttAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAg                                                                                                                      <  1:453428/148‑1 (MQ=255)

                       tAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTggg                                                                                                                  >  2:489400/1‑151 (MQ=255)

                        acaCCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGACTCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCa                                                                                                                       <  1:113695/143‑1 (MQ=255)

                             gCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAAt                                                                                                            <  1:606850/151‑1 (MQ=255)

                                       ccGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACtt                                                                                                   <  2:676580/150‑1 (MQ=255)

                                            ggtgATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAg                                                                                              <  2:433118/150‑1 (MQ=255)

                                                           ccACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTGTGCTGTCAGCTCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCgcgc                                                                                <  1:639369/149‑1 (MQ=255)

                                                                         tGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGcc                                                                >  1:608616/1‑151 (MQ=255)

                                                                         tGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGcc                                                                >  2:221521/1‑151 (MQ=255)

                                                                                  tCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAAc                                                       >  1:263590/1‑151 (MQ=255)

                                                                                         gAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTaa                                                 >  1:19873/1‑150 (MQ=255)

                                                                                          aaCCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTaaa                                                >  1:237050/1‑150 (MQ=255)

                                                                                            ccATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTaaa                                                >  2:163462/1‑148 (MQ=255)

                                                                                              aTGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAaaga                                             >  1:182050/1‑149 (MQ=255)

                                                                                                gCGAATCCTATCCCTATGATGAATGCCCAAATCAGGTTTGCTGTCAGCCCCCCCAACGGAATCGCCTGATGCAGTGGTTTAAATCGCATACTTAACAGCCATCTCGCCACGCTCCCCGTCCCACCGCCAATAAAAACTGCTAAAAGAAGtt                                         <  2:411205/151‑1 (MQ=255)

                                                                                                  gAATCTTATTCCTATGATGAATGCCCAAAGCAGGTTTGCTGTAAGCCCCCCCAACGGATCCGCCTGATGCAGTGGTTTAAACCGCATAATAAACACCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAaagaa                                            <  1:472370/146‑1 (MQ=255)

                                                                                                      cctattcctatGATGAATGCCCCAATCGGGTTTGCTGTCAGCGCCCCCAACGAAATCGCCTGATGCAGTGGGTTAAATCGCATCCTAAACACCCATCTCGCCCCGCTTCCCTTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAac                                    <  2:560940/150‑1 (MQ=255)

                                                                                                          ttcctatGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGc                               >  1:519189/1‑151 (MQ=255)

                                                                                                            cctatGATGAATGCCCCAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCa                              >  1:82250/1‑150 (MQ=255)

                                                                                                                           ccAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTatttg               >  1:49787/1‑150 (MQ=255)

                                                                                                                           ccAATCAGGTTTGCTGTCAGCGCCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTatttg               >  2:347810/1‑150 (MQ=255)

                                                                                                                                       gCTGTCAGCCCCCCAAACGGAATCGCCTGATGCAGGGGGTTAAACCTCATACTTAACAACCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAg    <  1:174722/149‑1 (MQ=255)

                                                                                                                                         tGTCAGCCCCCCAAGCGGAATCGCGTGATGCAGTGGTTTAAATCGCATATTTAACAGCCACCCCCCCGCGTTCCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTg  <  2:603396/149‑1 (MQ=255)

GCCGAAAATGTTGAGAAGGTTGTTAGACCGCCACAAAATCCGGTGGTGATTAATACTTTCCACACTGGATCAATGTTCGTCATCCTGCTGAACCATGCGAATCCTATTCCTATGATGAATGCCCCAATCAGGTTTGCTGTCAGCGTCCCCAACGGAATCGCCTGATGCAGTGGGTTAAATCGCATACTTAACAGCCATCTCGCCACGCTTCCCGTACCACCGCCAATAAAAACTGCTAAAAGAAGTTGTAACACTGCAAAATCCTGCTATTTGATTTGTATGAGTG  >  NC_000913/657685‑657970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A