Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F50 I2 R1	104	44.1	1376607	98.9%	1361464	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	783,506	C→T	A114V (GCG→GTG)	pnuC →	nicotinamide riboside transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	783,506	0	C	T	97.4%	136.8 / ‑5.5	38	A114V (GCG→GTG)	pnuC	nicotinamide riboside transporter
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (21/16); minor base G (1/0); total (22/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

TTCAGCTGTATGCCAGCCTGCTATTACAGGTGTTTTTCTTTGCCGCGAATATTTACGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGCGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTGCAACCGGACGCTTTCC  >  NC_000913/783359‑783643

ttCAGCTGTATGCCAGCCTGCTATTACAGGTGTTTTTCTTTGCCGCGAATATTTACGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTggtggt                                                                                                                                        >  1:680211/1‑151 (MQ=255)

           gCCAGCCTGCTATTACAGGTGTTTTTCTTTGCCGCGAATATTTACGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTGAGCTGGTTGGTGGTTTGCGTTGttt                                                                                                                             <  1:462209/151‑1 (MQ=255)

                  tGCTATTACAGGTGTTTTTCTTTGCCGCGAATATTTACGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTgg                                                                                                                      >  2:420634/1‑151 (MQ=255)

                   gCTATTACAGGTGTTTTTCTTTGCCGCGAATATTTACGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGt                                                                                                                     >  2:208636/1‑151 (MQ=255)

                      aTTACAGGTGTTTTTCTTTGCCGCGAATATTTACGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTc                                                                                                                    <  2:588079/149‑1 (MQ=255)

                                 ttttCTTTGCCGCGAATATTTACGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGTGGCTTGCGTTGTTTCGATTGGTCTGATGACGGTAtt                                                                                                       >  1:315753/1‑151 (MQ=255)

                                  tttCTTTGCCGCGAATATTTACGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTAtt                                                                                                       <  2:207573/150‑1 (MQ=255)

                                   ttCTTTGCCGCGAATATTTACGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTAttt                                                                                                      <  2:538085/150‑1 (MQ=255)

                                                 tatTTACGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGttt                                                                                       >  2:194835/1‑151 (MQ=255)

                                                  atTTACGGTTGGTATGCGTGGTCGCGACAAACGGGTCAGAACGAGGCGGGGTGGAGAATGCGGGGGGTGCCATGGCCGAAGGCACTCAGCTGGGGGGGGGTTTGCGTTGTTTCAATTGGAAAAATAAAAGCATTTATCAAACCGGTGttt                                                                                       >  1:416014/1‑150 (MQ=255)

                                                     tACGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCAt                                                                                   >  2:588090/1‑151 (MQ=255)

                                                      aCGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCAtt                                                                                  >  2:586684/1‑151 (MQ=255)

                                                      aCGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCAt                                                                                   <  2:618744/150‑1 (MQ=255)

                                                         gTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCAttttt                                                                               >  1:518225/1‑151 (MQ=255)

                                                         gTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCAttttt                                                                               >  1:620345/1‑151 (MQ=255)

                                                          ttGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTg                                                                              >  1:245530/1‑151 (MQ=255)

                                                           tGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTg                                                                              <  1:377106/150‑1 (MQ=255)

                                                               aTGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGAccc                                                                          >  1:249507/1‑150 (MQ=255)

                                                                tGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCcgc                                                                        >  1:273186/1‑151 (MQ=255)

                                                                tGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCcgc                                                                        >  2:210173/1‑151 (MQ=255)

                                                                 gCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCcgcg                                                                       <  2:337474/151‑1 (MQ=255)

                                                                    tGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTgg                                                                    >  2:29188/1‑151 (MQ=255)

                                                                    tGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTgg                                                                    >  1:230371/1‑151 (MQ=255)

                                                                     ggTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTgg                                                                    <  2:222180/150‑1 (MQ=255)

                                                                         gcgACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTc                                                               >  2:154264/1‑151 (MQ=255)

                                                                            aCAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCAt                                                             >  1:173781/1‑150 (MQ=255)

                                                                                  cAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATg                                                      >  1:115242/1‑151 (MQ=255)

                                                                                           cGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTa                                             <  1:307014/151‑1 (MQ=255)

                                                                                            gAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGGCGATGGCACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTCTGGTGACGGTATTTATCAAGCCGGTGTTTGCATTTTTGACCAGCGTGGCAGTCAGGATCATGCAAGCATTa                                             <  1:489040/150‑1 (MQ=255)

                                                                                                cGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGAtt                                        <  2:334422/151‑1 (MQ=255)

                                                                                                   aGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACa                                     <  2:231641/151‑1 (MQ=255)

                                                                                                      tGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAgg                                   <  2:305592/150‑1 (MQ=255)

                                                                                                              cGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGc                          >  2:573550/1‑151 (MQ=255)

                                                                                                              cGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGc                          >  1:296651/1‑151 (MQ=255)

                                                                                                               gCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGcc                         <  1:562399/151‑1 (MQ=255)

                                                                                                                       ccATTGCCGAAGGCACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTGc                 >  2:577240/1‑151 (MQ=255)

                                                                                                                                    cACTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTGCAACCGGACGCttt    >  2:493386/1‑151 (MQ=255)

                                                                                                                                     aCTCAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTGCAACCGGACGCTTTc   <  1:372583/151‑1 (MQ=255)

                                                                                                                                        cAGCTGGTTGGTGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTGCAACCGGACGCTTTcc  <  1:498320/149‑1 (MQ=255)

TTCAGCTGTATGCCAGCCTGCTATTACAGGTGTTTTTCTTTGCCGCGAATATTTACGGTTGGTATGCGTGGTCGCGACAAACCAGTCAGAACGAGGCGGAGTTGAAAATTCGCTGGTTGCCATTGCCGAAGGCACTCAGCTGGTTGGCGGTTTGCGTTGTTTCGATTGGTCTGATGACGGTATTTATCAATCCGGTGTTTGCATTTTTGACCCGCGTGGCAGTCATGATCATGCAAGCATTAGGATTACAGGTTGTGATGCCTGAACTGCAACCGGACGCTTTCC  >  NC_000913/783359‑783643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A