Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F50 I2 R1	104	44.1	1376607	98.9%	1361464	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	871,451	T→C	L162P (CTG→CCG)	gsiC →	glutathione ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	871,451	0	T	C	100.0%	94.0 / NA	26	L162P (CTG→CCG)	gsiC	glutathione ABC transporter permease
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (14/12); total (14/12)

GGCGGCGGGAATTATCGCCGCCGTCTGGCGTAACCGTTGGCCGGATCGATTGAGTATGACCATTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCTGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGCAGTGGCCGCCGTGATGGCGCGCTTTACCCGCGCGTCGTTTGTCGATGTTTTAAGCGAAGATTATATGCGTACCGCGAGGGCGA  >  NC_000913/871308‑871597

ggcggcGGGAATTATCGCCGCCGTCTGGCGTAACCGTTGGCCGGATCGATTGAGTATGACCATTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTAcc                                                                                                                                             <  2:436486/151‑1 (MQ=255)

ggcggcGGGAATTATCGCCGCCGTCTGGCGTAACCGTTGGCCGGATCGATTGAGTATGACCATTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTAcc                                                                                                                                             <  1:96760/151‑1 (MQ=255)

  cggcggGAATTATCGCCGCCGTCTGGCGTAACCGTTGGCCGGATCGATTGAGTATGACCATTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGt                                                                                                                                           <  1:281354/151‑1 (MQ=255)

     cggGAATTATCGCCGCCGTCTGGCGTAACCGTTGGCCGGATCGATTGAGTATGACCATTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTggg                                                                                                                                        >  1:641811/1‑151 (MQ=255)

               cgccgcCGTCTGGCGTAACCGTTGGCCGGATCGATTGAGTATGACCATTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGt                                                                                                                              >  2:315632/1‑151 (MQ=255)

                           gCGTAACCGTTGGCCGGATCGATTGAGTATGACCATTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTAc                                                                                                                  >  2:11789/1‑151 (MQ=255)

                                aCCGTTGGCCGGATCGATTGAGTATGACCATTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACAtttt                                                                                                             <  1:172293/151‑1 (MQ=255)

                                aCCGTTGGCCGGATCGATTGAGTATGACCATTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACAtttt                                                                                                             <  1:261695/151‑1 (MQ=255)

                                  cGTTGGCCGGATCGATTGAGTATGACCATTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTAc                                                                                                           <  2:74622/151‑1 (MQ=255)

                                         cGGATCGATTGAGTATGACCATTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTAccctccct                                                                                                    >  1:257261/1‑151 (MQ=255)

                                          ggATCGATTGAGTATGACCATTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTg                                                                                                   <  1:570916/151‑1 (MQ=255)

                                              cGATTGAGTATGACCATTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACg                                                                                                <  1:345267/150‑1 (MQ=255)

                                                        tGACCATTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGc                                                                                     >  2:475005/1‑151 (MQ=255)

                                                             aTTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGCAGTgg                                                                                >  1:191002/1‑151 (MQ=255)

                                                                 cGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGCAGTGgccgc                                                                            >  1:196462/1‑151 (MQ=255)

                                                                                     ccGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGCAGTGGCCGCCGTGATGGCGCGCTTTAccc                                                        <  2:252349/151‑1 (MQ=255)

                                                                                     ccGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGCAGTGGCCGCCGTGATGGCGCGCTTTAccc                                                        >  1:438483/1‑151 (MQ=255)

                                                                                              gCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGCAGTGGCCGCCGTGATGGCGCGCTTTACCCGCGcgtcgt                                               <  2:454376/151‑1 (MQ=255)

                                                                                                     ggATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGCAGTGGCCGCCGTGATGGCGCGCTTTACCCGCGCGTCGTTTGTCg                                         >  2:30084/1‑150 (MQ=255)

                                                                                                          cTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGCAGTGGCCGCCGTGATGGCGCGCTTTACCCGCGCGTCGTTTGTCGATGttt                                   >  1:41837/1‑151 (MQ=255)

                                                                                                            tttAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGCAGTGGCCGCCGTGATGGCGCGCTTTACCCGCGCGTCGTTTGTCGATGtttt                                  >  2:681308/1‑150 (MQ=255)

                                                                                                              tAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGCAGTGGCCGCCGTGATGGCGCGCTTTACCCGCGCGTCGTTTGTCGATGTTTTaa                                <  2:124604/150‑1 (MQ=255)

                                                                                                              tAATTCAGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGCAGTGGCCGCCGTGATGGCGCGCTTTACCCGCGCGTCGTTTGTCGATGTTTTaa                                <  1:76540/150‑1 (MQ=255)

                                                                                                                    aGGTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGCAGTGGCCGCCGTGATGGCGCGCTTTACCCGCGCGTCGTTTGTCGATGTTTTAAGCGa                            >  1:512726/1‑148 (MQ=255)

                                                                                                                      gTATTCTCCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCACTGACGCTCGGCGCGGCAGTGGCCGCCGTGATGGCGCGCTTTACCCGCGCGTCGTTTGTCGATGTTTTAAGCGAAGAt                        >  1:54014/1‑150 (MQ=255)

                                                                                                                          tctcCGTTGAACTGGGCTGGCCGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGCAGTGGCCGCCGTGATGGCGCGCTTTACCCGCGCGTCGTTTGTCGATGTTTTAAGCGAAGATTatat                   >  1:228175/1‑151 (MQ=255)

                                                                                                                                            ggccgCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGCAGTGGCCGCCGTGATGGCGCGCTTTACCCGCGCGTCGTTTGTCGATGTTTTAAGCGAAGATTATATGCGTACCGCGAGGGCGa  <  2:477383/150‑1 (MQ=255)

GGCGGCGGGAATTATCGCCGCCGTCTGGCGTAACCGTTGGCCGGATCGATTGAGTATGACCATTGCGGTGTCGGGGATCTCGTTTCCGGCATTTGCTCTGGGGATGCTTTTAATTCAGGTATTCTCCGTTGAACTGGGCTGGCTGCCTACCGTGGGAGCAGACAGTTGGCAGCACTACATTTTACCCTCCCTGACGCTCGGCGCGGCAGTGGCCGCCGTGATGGCGCGCTTTACCCGCGCGTCGTTTGTCGATGTTTTAAGCGAAGATTATATGCGTACCGCGAGGGCGA  >  NC_000913/871308‑871597

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A