Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F50 I2 R1	104	44.1	1376607	98.9%	1361464	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	977,322	G→A	A333T (GCC→ACC)	mukB →	chromosome condensin MukBEF, ATPase and DNA‑binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	977,322	0	G	A	100.0%	129.5 / NA	33	A333T (GCC→ACC)	mukB	chromosome condensin MukBEF, ATPase and DNA‑binding subunit
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (15/18); total (15/18)

CCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGGTGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATG  >  NC_000913/977174‑977469

ccATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGGTGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGAcc                                                                                                                                                   <  2:207390/151‑1 (MQ=255)

          aaaGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGGTGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGACCAGTGATCAcc                                                                                                                                         >  2:196942/1‑151 (MQ=255)

           aaGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGGTGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGACCAGTGATCACCt                                                                                                                                        <  2:184922/151‑1 (MQ=255)

              cccTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGGTGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGACCAGTGATCACCTGa                                                                                                                                      >  2:337118/1‑150 (MQ=255)

              cccTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGGTGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGACCAGTGATCACCTGa                                                                                                                                      >  2:250138/1‑150 (MQ=255)

                 tGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGGTGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGACCAGTGATCACCTGAACCt                                                                                                                                  >  2:401029/1‑151 (MQ=255)

                         cgtcgCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGGTGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGACCAGTGATCACCTGAACCTGGTGCaaa                                                                                                                          <  2:105944/151‑1 (MQ=255)

                         cgtcgCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGGTGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGACCAGTGATCACCTGAACCTGGTGCaa                                                                                                                           <  2:330458/150‑1 (MQ=255)

                         cgtcgCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGGTGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGACCAGTGATCACCTGAACCTGGTGCa                                                                                                                            >  1:79170/1‑149 (MQ=255)

                            cgcgAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGGTGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGACCAGTGATCACCTGAACCTGGTGCAAAcc                                                                                                                        <  2:675436/150‑1 (MQ=255)

                                  cTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGGTGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGACCAGTGATCACCTGAACCTGGTGCAAACCGCACTGc                                                                                                                 >  2:684066/1‑151 (MQ=255)

                                    aCATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGGTGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGACCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGt                                                                                                               >  1:520649/1‑151 (MQ=255)

                                      aTACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGGTGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGACCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTc                                                                                                              <  1:141771/150‑1 (MQ=255)

                                        aCTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGGTGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGACCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTcagc                                                                                                           >  1:83465/1‑151 (MQ=255)

                                           tCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGGTGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGACCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAgg                                                                                                        >  1:261263/1‑151 (MQ=255)

                                                 cAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGGTGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGACCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGgaga                                                                                                     >  1:48090/1‑148 (MQ=255)

                                                 cAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGGTGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGACCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGagaag                                                                                                   <  2:347848/150‑1 (MQ=255)

                                                     aaCTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGGTGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGACCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCg                                                                                               >  2:139962/1‑150 (MQ=255)

                                                     aaCTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGGTGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGACCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCg                                                                                               >  1:330434/1‑150 (MQ=255)

                                                      aCTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGGTGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGACCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGaa                                                                                             <  2:64303/151‑1 (MQ=255)

                                                                 aGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGGTGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGACCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGc                                                                                  <  2:192648/151‑1 (MQ=255)

                                                                           acacGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGGTGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGACCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGAt                                                                        <  1:171524/151‑1 (MQ=255)

                                                                             acGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGGTGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGACCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGa                                                                      <  1:585920/151‑1 (MQ=255)

                                                                                  gATATGGCGCGTGAGCTGGCAGAGCACAACGGTGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGACCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGc                                                                 <  2:486702/151‑1 (MQ=255)

                                                                                    tatGGCGCGTGAGCTGGCAGAGCACAACGGTGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGACCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGc                                                                 >  2:600382/1‑149 (MQ=255)

                                                                                       ggCGCGTGAGCTGGCAGAGCACAACGGTGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGACCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGAt                                                             <  1:291614/150‑1 (MQ=255)

                                                                                                    gCAGAGCACAACGGTGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGACCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGc                                               <  1:688275/151‑1 (MQ=255)

                                                                                                           acaACGGTGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGACCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATg                                         >  2:317558/1‑150 (MQ=255)

                                                                                                                    ccGAAGGCGATCTGGAAGCGGATTATCAGGCGACCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGc                               >  2:253314/1‑151 (MQ=255)

                                                                                                                    ccGAAGGCGATCTGGAAGCGGATTATCAGGCGACCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGc                               <  2:517854/151‑1 (MQ=255)

                                                                                                                     cGAAGGCGATCTGGAAGCGGATTATCAGGCGACCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCa                              <  1:34518/151‑1 (MQ=255)

                                                                                                                                            aTCAGGCGACCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAag        <  2:11540/150‑1 (MQ=255)

                                                                                                                                               aGGCGACCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGaa    <  1:636161/151‑1 (MQ=255)

                                                                                                                                                 gcgacCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATg  <  2:295672/151‑1 (MQ=255)

CCATCTCGACAAAGCCCTGGAGTTTCGTCGCGAGCTACATACTTCGCGTCAGCAACTGGCGGCTGAGCAGTACAAACACGTCGATATGGCGCGTGAGCTGGCAGAGCACAACGGTGCCGAAGGCGATCTGGAAGCGGATTATCAGGCGGCCAGTGATCACCTGAACCTGGTGCAAACCGCACTGCGTCAGCAGGAGAAGATCGAACGCTACGAAGCGGATCTCGATGAGCTGCAGATCCGTCTGGAAGAGCAAAATGAAGTGGTGGCAGAAGCCATCGAACGCCAGCAAGAGAATG  >  NC_000913/977174‑977469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A