Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	980,655	A→G	T1444A (ACC→GCC)	mukB →	chromosome condensin MukBEF, ATPase and DNA‑binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	980,655	0	A	G	100.0%	136.6 / NA	37	T1444A (ACC→GCC)	mukB	chromosome condensin MukBEF, ATPase and DNA‑binding subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (13/24); total (13/24)

AAAGATATCTCTCCTTGCCGCCTGCTGTTCCTCGATGAAGCAGCGCGACTGGATGCTCGTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGC  >  NC_000913/980508‑980801

aaaGATATCTCTCCTTGCCGCCTGCTGTTCCTCGATGAAGCAGCGCGACTGGATGCTCGTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGcgcc                                                                                                                                                  >  1:446108/1‑150 (MQ=255)

           tcCTTGCCGCCTGCTGTTCCTCGATGAAGCAGCGCGACTGGATGCTCGTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCGCCACCTATAAACTg                                                                                                                                      >  2:232351/1‑151 (MQ=255)

           tcCTTGCCGCCTGCTGTTCCTCGATGAAGCAGCGCGACTGGATGCTCGTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCGCCACCTATAAACTg                                                                                                                                      >  2:277896/1‑151 (MQ=255)

                     ctgctgTTCCTCGATGAAGCAGCGCGACTGGATGCTCGTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCGCCACCTATAAACTGGTGCGTaa                                                                                                                              >  1:6009/1‑149 (MQ=255)

                         tgTTCCTCGATGAAGCAGCGCGACTGGATGCTCGTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCGCCACCTATAAACTGGTGCGTAAAGTCtt                                                                                                                        >  2:349260/1‑151 (MQ=255)

                          gTTCCTCGATGAAGCAGCGCGACTGGATGCTCGTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCGCCACCTATAAACTGGTGCGTAAAGTCt                                                                                                                         <  2:485935/149‑1 (MQ=255)

                          gTTCCTCGATGAAGCAGCGCGACTGGATGCTCGTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCGCCACCTATAAACTGGTGCGTAAAGTCTTc                                                                                                                       <  1:495633/151‑1 (MQ=255)

                             ccTCGATGAAGCAGCGCGACTGGATGCTCGTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCGCCACCTATAAACTGGTGCGTAAAGTCTTCCa                                                                                                                     <  1:526039/150‑1 (MQ=255)

                                  aTGAAGCAGCGCGACTGGATGCTCGTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCGCCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATAc                                                                                                               <  1:476195/151‑1 (MQ=255)

                                              gACTGGATGCTCGTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCGCCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTc                                                                                                    >  2:414021/1‑150 (MQ=255)

                                               aCTGGATGCTCGTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCGCCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCAt                                                                                                  >  1:23738/1‑151 (MQ=255)

                                               aCTGGATGCTCGTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCGCCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCAt                                                                                                  >  1:482007/1‑151 (MQ=255)

                                                          gTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCGCCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCt                                                                                       >  2:384379/1‑151 (MQ=255)

                                                          gTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCGCCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCt                                                                                       <  2:569911/151‑1 (MQ=255)

                                                          gTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCGCCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCt                                                                                       <  1:253050/151‑1 (MQ=255)

                                                               aTCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCGCCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGc                                                                                     <  2:519858/148‑1 (MQ=255)

                                                                       gCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCGCCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTgcg                                                                          >  2:31543/1‑151 (MQ=255)

                                                                            ttGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCGCCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGcgccgc                                                                      >  2:465061/1‑150 (MQ=255)

                                                                                           gTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCGCCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCt                                                      <  2:377158/151‑1 (MQ=255)

                                                                                               gCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCGCCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTcc                                                   >  1:174498/1‑150 (MQ=255)

                                                                                                     gCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCGCCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACt                                            <  2:418594/151‑1 (MQ=255)

                                                                                                                   cAGCGCCGGAAAATATCAGCCCGGAGAAAGGCGCCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTtc                              <  2:44951/151‑1 (MQ=255)

                                                                                                                    aGCGCCGGAAAATATCAGCCCGGAGAAAGGCGCCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTtct                             <  1:542398/151‑1 (MQ=255)

                                                                                                                        ccGGAAAATATCAGCCCGGAGAAAGGCGCCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAgg                         <  1:408499/151‑1 (MQ=255)

                                                                                                                          ggAAAATATCAGCCCGGAGAAAGGCGCCTCCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCg                       <  1:277903/151‑1 (MQ=255)

                                                                                                                                tatCAGCCCGGAGAAAGGCGCCACCTATAAACTGGAGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGtt                   >  2:533620/1‑149 (MQ=255)

                                                                                                                                   cAGCCCGGAGAAAGGCGCCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAAt              <  2:244764/151‑1 (MQ=255)

                                                                                                                                   cAGCCCGGAGAAAGGCGCCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAAt              <  2:349441/151‑1 (MQ=255)

                                                                                                                                   cAGCCCGGAGAAAGGCGCCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAAt              <  1:215099/151‑1 (MQ=255)

                                                                                                                                     gCCCGGAGAAAGGCGCCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTa            <  1:10675/151‑1 (MQ=255)

                                                                                                                                      cccGGAGAAAGGCGCCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTaa           <  1:8885/151‑1 (MQ=255)

                                                                                                                                       ccGGAGAAAGGCGCCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAg          <  2:118156/151‑1 (MQ=255)

                                                                                                                                         ggAGAAAGGCGCCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAggc        <  1:216598/151‑1 (MQ=255)

                                                                                                                                          gagaAAGGCGCCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAggcg       <  2:422224/151‑1 (MQ=255)

                                                                                                                                          gagaAAGGCGCCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAggcg       <  1:349267/151‑1 (MQ=255)

                                                                                                                                              aaGGCGCCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAggcggc     >  1:565399/1‑149 (MQ=255)

                                                                                                                                               aGGCGCCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGgcagc  <  1:193661/151‑1 (MQ=255)

                                                                                                                                               aGGCGCCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGgcagc  <  2:571106/151‑1 (MQ=255)

AAAGATATCTCTCCTTGCCGCCTGCTGTTCCTCGATGAAGCAGCGCGACTGGATGCTCGTTCTATCGCCACGCTGTTTGAATTGTGTGAGCGTTTGCAAATGCAACTCATCATCGCAGCGCCGGAAAATATCAGCCCGGAGAAAGGCACCACCTATAAACTGGTGCGTAAAGTCTTCCAGAATACCGAACACGTTCATGTCGTCGGCCTGCGAGGATTTGCGCCGCAACTCCCTGAAACGCTTCCAGGAACTGACGAAGCGCCTTCTCAGGCGAGTTAAAATTAAGGCGGCAGC  >  NC_000913/980508‑980801

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A