Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F50 I3 R1 160 117.2 4331776 85.4% 3699336 149.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 981,315 A→G Y90C (TAT→TGT)  ldtD → murein L,D‑transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913981,3150AG95.8% 314.3 / ‑5.3 96Y90C (TAT→TGT) ldtDmurein L,D‑transpeptidase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (41/50);  minor base T (4/0);  total (46/50)
Fisher's exact test for biased strand distribution p-value = 4.68e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

GGTGGCTGTCAGTGAACAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCAGCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTATC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTCCAGCCGCAGTTTAATAAATGGGTAGAGTT  >  NC_000913/981169‑981459
                                                                                                                                                    |                                                                                                                                                 
ggTGGCTGTCAGTGAACAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGGTCCCGCCACTGCCTGAAAGGGC‑GGGAGAAAAAACCCGCACCCCAAACGAATTTCCATTACCCGCA‑GGGTACAAACCGGGTTGTC‑tt                                                                                                                                              >  2:48684/1‑151 (MQ=255)
 gTGGCTGTCAGTGAACAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGGGCCCGCCAATGCCTGAAGGGGG‑GGCAGAAAAAACCCCCACGCAAAACGAATCTCAATTAACCGCA‑GGGTACAAGCCGGGTTGTC‑tt                                                                                                                                              >  2:373842/1‑150 (MQ=255)
  tGGCTGTCAGTGAACAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCAGCCCATTCCTGAAGGGGC‑GGCAGAAAAAAACCGCACCCCAAACGAATCTCAATTAACCGCC‑GGGTACAAACCGGTTTGTC‑ttt                                                                                                                                             >  4:596989/1‑149 (MQ=255)
   ggCTGTCAGTGAACAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCAGCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTaa                                                                                                                                            <  2:850708/150‑1 (MQ=255)
     cTGTCAGTGAACAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGGTCCCGCCAGTGCCTGAAGGGGC‑GGGAGAAAAAACCCGCACGCAAATCGCATCTCAAATAACCCCAGGGTTAAAACCCGGTTT‑TC‑TTAAcc                                                                                                                                          >  4:1060612/1‑150 (MQ=255)
      tGTCAGTGAACAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGGTGCAGCCATTGCCTGAAGGGGC‑GGCAGAAAAAACCCGCACGCCAAATGAATCTCAATTACCCGCC‑GGGTAACAACCGGGTTGTC‑TTAACCa                                                                                                                                         >  4:1167571/1‑150 (MQ=255)
      tGTCAGTGAACAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGGTCCAGCCAATGCCTCAAAGGGC‑GGCAGAAAAAACCCGCCCGCAAAACGAATATCCATTACCCCCC‑GGGTACAAAACGGTTTTTC‑TTaaac                                                                                                                                          >  1:387909/1‑147 (MQ=255)
       gTCAGTGAACAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCAGCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCa                                                                                                                                         <  1:370915/149‑1 (MQ=255)
         cAGTGAACAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGGGCCCACCAATGCCTGAAGGAGCGGGCGGAAAAAACCC‑CCCCCAAAACGAAACCCAATTAACCCCA‑GGGTGCAAAACGGGTTGTC‑tttaacaacct                                                                                                                                     >  1:142065/1‑142 (MQ=255)
         cAGTGAACAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCAGCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCt                                                                                                                                      <  3:230728/150‑1 (MQ=255)
             gAACAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCAGCCATTGCCTGAGGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAAc                                                                                                                                 <  4:1109027/151‑1 (MQ=255)
              aaCAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGGACCCGCCACTTCCTGAAGGGGC‑GGGAGAAAAAACCCGCCCGCAAATAGAATTTCAATTACCCCCA‑GGGTACAAGCCGGGTTTTT‑TTAACCAGCTTCCAc                                                                                                                                 >  1:394450/1‑150 (MQ=255)
               aCAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCAGCCCTTTCCTTAAGGTGC‑GGCAGAAAAAGCCCGCACGCCAATCGAATCTCAATTACCCCCC‑GGGTACAAACCCGGTTGTC‑TTTACCAGCTTCaa                                                                                                                                  >  3:133934/1‑148 (MQ=255)
               aCAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCAGCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACtg                                                                                                                               <  3:350807/151‑1 (MQ=255)
                taGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGGTCCAGCCATTGCCTGAAGGGGC‑GGCAGAAAAAACCCGCACGCAAAACGAATCTCAATTACCCGCC‑GGGTACAAACCGGGTTTTC‑TTAAACAGATTTAACtgt                                                                                                                              >  2:718288/2‑151 (MQ=255)
                cAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCAGCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACtgt                                                                                                                              <  2:653900/151‑1 (MQ=255)
                 aGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCCACCCTTTCCTTAAAGAGG‑GGGCGAAAAAGACCCCCCGCAAAACGAAACTCAATTATCCGCA‑GGGTACAAACCGGGTTTTC‑TTAACCAGCTTCAACtgtt                                                                                                                             >  4:703974/1‑151 (MQ=255)
                  gggCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCAGCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACtgttg                                                                                                                            <  1:430505/151‑1 (MQ=255)
                  gggCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCAGCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACtgttg                                                                                                                            <  4:1439959/151‑1 (MQ=255)
                        ggCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCAGCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATg                                                                                                                        <  3:202363/149‑1 (MQ=255)
                         gCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCAGCCATTGCCTGAAAGGGC‑GGCAGAAAAAACCCGCACGCCAATCGAATCTCAATTACCCGCA‑GGGTACAAGCCGGGTTGTC‑TTAACCAGCTTCAACTGTTGTATGcc                                                                                                                      >  4:183015/1‑150 (MQ=255)
                           aCTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGGTCCAACCACTGCCTGAAGGTGG‑GGCCGAAAAAACCCCCACGCCAAACGAATCTCAATTACCCCCC‑GGTTACAAACCCGGTTGTC‑TTAACCAACTTCAAATGTTGTTTGCCGc                                                                                                                    >  2:301145/1‑150 (MQ=255)
                            cTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCAGCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCa                                                                                                                   <  4:235687/150‑1 (MQ=255)
                             tGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCAACCATTGCCTGAAAGGGC‑GGCAGAAAAAACCCGCACGCAAATAGAAACTCAATTAACCGCA‑GGGTACAAACCCGGTTGTCTTTAACAACCTTC‑ACTGTTGTATGcccc                                                                                                                    >  4:1111239/1‑146 (MQ=255)
                              gCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGGTCCAGCCCCTGCCTGAAAGGGG‑GGCGGAAAAAACCCCCACGCAAAACGAATCTCAATTACCCCCC‑GGGTACAAACCGGGTTGTT‑TTTAACAACTTTAACTGTTTTTTGCCggcc                                                                                                                  >  2:1305610/1‑146 (MQ=255)
                              gCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCAGCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCAcgc                                                                                                                <  4:34419/151‑1 (MQ=255)
                              gCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCAGCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCAcgc                                                                                                                <  2:616914/151‑1 (MQ=255)
                                cGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCAGCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGa                                                                                                              <  4:731243/151‑1 (MQ=255)
                                    ggCGCAAGCCACGGCAATAATGGCGGGGATCCAGCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATg                                                                                                          <  2:183196/151‑1 (MQ=255)
                                        cAAGCCACGGCAATAATGGCGGGGGTCCAACCATTTCCTGAAGGGGC‑GGCCGAAAAAACCCGCCCGCAAAACGAATCACAATTACCCGCA‑GGGTAAAAGCCGGGTTGTC‑TTAACCAACTTTAACTGTTGTATGCCGCACGCGGTATGCaa                                                                                                       >  4:59274/1‑150 (MQ=255)
                                         aaGCCACGGCAATAATGGCGGGGATCCAGCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAAcc                                                                                                     <  1:98214/151‑1 (MQ=255)
                                         aaGCCACGGCAATAATGGCGGGGAGCCAGCCACTGCCCTAATAAGG‑GGGCGAAAAAGACCGCACGCAAAACGAATCTCCATTACCCGCA‑GGGTAAAAAACGGGTTGTG‑TTAACAAGCTTCAAATTTTGTATGCCGCACGCGATATCAAAcc                                                                                                     >  2:1641842/1‑151 (MQ=255)
                                            ccACGGCAATAATGGCGGGGATCCAGCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCAt                                                                                                  <  1:428272/151‑1 (MQ=255)
                                              aCGGCAATAATGGCGGGGATCCAGCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCAtgt                                                                                                <  2:1279896/151‑1 (MQ=255)
                                               cGGCAATAATGGCGGGGATCCAGCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCAtgt                                                                                                <  2:704543/150‑1 (MQ=255)
                                                 gCAATAATGGCGGGGATCCAGCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTggg                                                                                             <  2:1001745/151‑1 (MQ=255)
                                                  cAATAATGGCGGGGATCCAGCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGa                                                                                            <  1:366770/151‑1 (MQ=255)
                                                   aataatGGCGGGGATCCAGCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGaa                                                                                           <  2:995132/151‑1 (MQ=255)
                                                   aataatGGCGGGGATCCAGCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGaa                                                                                           <  2:775427/151‑1 (MQ=255)
                                                     taatGGCGGGGATCCAGCCACTGCCTTAAGGGGG‑GGCCGAAAAAACCCCCACGCCAAACGAAACTCAATTAACCGCC‑GGGTTCAAACCGGGTTGTC‑TTAACCAACCTCAACTGTTGTATGCCGCACGCCGTATGCAACCCCAGTGGGgaa                                                                                          >  1:22559/1‑150 (MQ=255)
                                                      aatGGCGGGGATCCAGCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGaaaa                                                                                         <  4:1303687/150‑1 (MQ=255)
                                                         ggCGGGGATCCAGCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCCCACGCAATTCGAATCCCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAATTGTTGTAGGCCGCACGCGATATGCAACCCATGGGGGAAAACCg                                                                                      >  4:1128525/1‑150 (MQ=255)
                                                          gCGGGGATCCAGCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTg                                                                                    >  1:127365/1‑151 (MQ=255)
                                                           cGGGGATCCAGCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGa                                                                                   <  2:433113/151‑1 (MQ=255)
                                                             gggATCCAGCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATg                                                                                 >  4:1233155/1‑151 (MQ=255)
                                                               gATCCAGCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCt                                                                               <  3:103850/151‑1 (MQ=255)
                                                                  ccAGCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGtt                                                                            >  4:995820/1‑151 (MQ=255)
                                                                   cAGCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGtt                                                                            >  2:819785/1‑150 (MQ=255)
                                                                    aGCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTaa                                                                          <  3:409892/151‑1 (MQ=255)
                                                                     gCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTaaa                                                                         <  4:821378/151‑1 (MQ=255)
                                                                               gAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAg                                                                <  2:1117263/150‑1 (MQ=255)
                                                                               gAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGc                                                               >  1:107866/1‑151 (MQ=255)
                                                                                 aGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCa                                                              >  1:30470/1‑150 (MQ=255)
                                                                                      c‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCt                                                        >  2:1431905/1‑151 (MQ=255)
                                                                                               aaaGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAgg                                                >  2:1490936/1‑151 (MQ=255)
                                                                                                aaGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGt                                               >  2:483628/1‑151 (MQ=255)
                                                                                                 aGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTg                                              >  4:160038/1‑151 (MQ=255)
                                                                                                   cccGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGc                                            <  2:1569801/151‑1 (MQ=255)
                                                                                                    ccGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCg                                           >  4:1325243/1‑151 (MQ=255)
                                                                                                     cgcacgcaAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCg                                           >  2:1482945/1‑150 (MQ=255)
                                                                                                     cgcacgcaAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCg                                           >  2:1156537/1‑150 (MQ=255)
                                                                                                       cacgcaAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGAtt                                        <  2:1260588/151‑1 (MQ=255)
                                                                                                           caAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGcca                                    <  2:106238/151‑2 (MQ=255)
                                                                                                            aaaTCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCgg                                   >  1:148708/1‑151 (MQ=255)
                                                                                                             aaTCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGt                                  <  3:287170/151‑1 (MQ=255)
                                                                                                                cGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTc                               <  3:106095/151‑1 (MQ=255)
                                                                                                                 gAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTcc                              >  4:1163676/1‑151 (MQ=255)
                                                                                                                  aaTCTCAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTCCa                             <  4:1590579/151‑1 (MQ=255)
                                                                                                                      tcAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTCCAGCCg                         >  2:80852/1‑151 (MQ=255)
                                                                                                                       cAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTCCAg                            <  2:857259/147‑1 (MQ=255)
                                                                                                                       cAATTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTCCAGcc                          <  3:85917/149‑1 (MQ=255)
                                                                                                                        aaTTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTCCAGCCGc                        >  2:144960/1‑150 (MQ=255)
                                                                                                                        aaTTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTCCAGCCGCa                       <  2:1323590/151‑1 (MQ=255)
                                                                                                                        aaTTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTCCAGCCGCa                       <  3:265713/151‑1 (MQ=255)
                                                                                                                         aTTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTCCAGCCGc                        >  4:559415/1‑149 (MQ=255)
                                                                                                                         aTTACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCAGGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTCCAGc                           <  4:1653428/146‑1 (MQ=255)
                                                                                                                          ttACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTCCAGCCGCAGt                     >  2:1246708/1‑151 (MQ=255)
                                                                                                                           tACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTCCAGCCGCAGtt                    >  4:408332/1‑151 (MQ=255)
                                                                                                                           tACCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTCCAGCCGCAGt                     <  4:1535714/150‑1 (MQ=255)
                                                                                                                            aCCCGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTCCAGCCGCAGttt                   >  3:295375/1‑151 (MQ=255)
                                                                                                                             cccGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTCCAGCCGCAGTTta                  <  2:596167/151‑1 (MQ=255)
                                                                                                                             cccGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTCCAGCCGCAGTTta                  <  4:629593/151‑1 (MQ=255)
                                                                                                                             cccGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTCCAGCCGCAGTTta                  <  4:1168570/151‑1 (MQ=255)
                                                                                                                              ccGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTCCAGCCGCAGTTtaa                 <  1:358664/151‑1 (MQ=255)
                                                                                                                               cGCA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTCCAGCCGCAGTTtaat                >  1:337068/1‑151 (MQ=255)
                                                                                                                                 cA‑GGTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTCCAGCCGCAGTTtaataa              <  4:306244/151‑1 (MQ=255)
                                                                                                                                    ggTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTCCAGCCGCAGTTTAATAAAt            >  3:324921/1‑151 (MQ=255)
                                                                                                                                    ggTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTCCAGCCGCAGTTTAATAAAt            <  2:1450756/151‑1 (MQ=255)
                                                                                                                                     gTTACAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTCCAGCCGCAGTTTAATAAATg           <  4:48779/151‑1 (MQ=255)
                                                                                                                                        aCAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTCCAGCCGCAGTTTAATAAATggg         <  2:1621756/150‑1 (MQ=255)
                                                                                                                                        aCAAGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTCCAGCCGCAGTTTAATAAATGGGt        >  2:1184724/1‑151 (MQ=255)
                                                                                                                                           aGCCGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTCCAGCCGCAGTTTAATAAATGGGTaga     <  1:353823/151‑1 (MQ=255)
                                                                                                                                             ccGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTCCAGCCGCAGTTTAATAAATGGGTAGAGt   >  1:89369/1‑151 (MQ=255)
                                                                                                                                             ccGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTCCAGCCGCAGTTTAATAAATGGGTAGAGt   <  2:1109833/151‑1 (MQ=255)
                                                                                                                                              cGGTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTCCAGCCGCAGTTTAATAAATGGGTAGAGtt  >  2:1450343/1‑151 (MQ=255)
                                                                                                                                               ggTTTGTC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTCCAGCCGCAGTTTAATAAATGGGTAGAGtt  >  2:964526/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GGTGGCTGTCAGTGAACAGGGCGAGGCACTGCCGCAGGCGCAAGCCACGGCAATAATGGCGGGGATCCAGCCATTGCCTGAAGGTGC‑GGCAGAAAAAGCCCGCACGCAAATCGAATCTCAATTACCCGCA‑GGTTACAAGCCGGTTTATC‑TTAACCAGCTTCAACTGTTGTATGCCGCACGCGATATGCAACCCATGTGGGAAAACCGTGATGCTGTTAAAGCCTTCCAGCAACAGCTGGCAGAGGTGGCGATTGCCGGTTTCCAGCCGCAGTTTAATAAATGGGTAGAGTT  >  NC_000913/981169‑981459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A