Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F50 I2 R1	104	44.1	1376607	98.9%	1361464	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,297,386	A→G	C246R (TGT→CGT)	adhE ←	fused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,297,386	0	A	G	100.0%	119.4 / NA	32	C246R (TGT→CGT)	adhE	fused acetaldehyde‑CoA dehydrogenase/iron‑dependent alcohol dehydrogenase/pyruvate‑formate lyase deactivase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/12); total (20/12)

CGTTCAGCGCACCGTTTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTT  >  NC_000913/1297242‑1297523

cgTTCAGCGCACCGTTTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTAc                                                                                                                                     >  2:204004/1‑151 (MQ=255)

                 ttCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGttt                                                                                                                    >  2:557197/1‑151 (MQ=255)

                   cAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTg                                                                                                                  <  2:659654/151‑1 (MQ=255)

                    aGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTgg                                                                                                                 >  1:354507/1‑151 (MQ=255)

                       aTAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGAc                                                                                                               >  1:296028/1‑150 (MQ=255)

                       aTAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACa                                                                                                              <  2:318324/151‑1 (MQ=255)

                       aTAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACa                                                                                                              <  1:63651/151‑1 (MQ=255)

                          aCATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATc                                                                                                            >  2:252364/1‑150 (MQ=255)

                            aTCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGt                                                                                                         >  2:229501/1‑151 (MQ=255)

                                  aaCAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGAt                                                                                                   <  2:663203/151‑1 (MQ=255)

                                       cTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAAc                                                                                              >  2:415678/1‑151 (MQ=255)

                                                  ttACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGttt                                                                                     <  2:46706/149‑1 (MQ=255)

                                                  ttACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGtt                                                                                      <  2:210079/148‑1 (MQ=255)

                                                      ccTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATc                                                                               >  2:579452/1‑151 (MQ=255)

                                                      ccTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATc                                                                               >  1:577599/1‑151 (MQ=255)

                                                          cAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATcagc                                                                            >  2:400077/1‑150 (MQ=255)

                                                                     cgccgTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGAt                                                                <  1:136047/151‑1 (MQ=255)

                                                                         gTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATaac                                                             >  2:229861/1‑150 (MQ=255)

                                                                           gggTTGCAAAACGTTCACATACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATaacaac                                                          <  1:121057/151‑1 (MQ=255)

                                                                               tGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTgg                                                       <  1:675251/150‑1 (MQ=255)

                                                                                     aCGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTg                                                <  1:359170/151‑1 (MQ=255)

                                                                                             gTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGcc                                        >  1:653050/1‑151 (MQ=255)

                                                                                              tACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCt                                       >  2:442518/1‑151 (MQ=255)

                                                                                                 aGCGTCATAAACAGAGTCAACAACAACAACATGCTCTTCAGCACCACGGATTACGCCGTCGTCGAAGGTTTTGAACATCAGAACAGATACAACTGCACGTTTGATATCAGCATCTTCATTGATAACAACTGGGGTGCTGCCCGCGccacc                                     >  2:257687/1‑147 (MQ=255)

                                                                                                      cATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGAt                               >  2:487750/1‑151 (MQ=255)

                                                                                                       aTAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGAt                               >  2:423084/1‑150 (MQ=255)

                                                                                                            cAGAGTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTgg                         >  1:515947/1‑151 (MQ=255)

                                                                                                               agTCAACAACAACAACAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGttt                      <  2:544123/151‑1 (MQ=255)

                                                                                                                  agtcaacaacaacaGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTAcc                   <  1:686138/148‑1 (MQ=255)

                                                                                                                   aacaacaacaacAGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCg                  >  1:339961/1‑151 (MQ=255)

                                                                                                                              caGACTGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGc       >  1:232525/1‑151 (MQ=255)

                                                                                                                                   tGTTCAGAAGCACGGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCtt  >  2:221266/1‑151 (MQ=255)

CGTTCAGCGCACCGTTTTTCAGGATAACATCCTGAACAGCTTTCAGCTCTTTACCCTGCAACAGATAGCCGCCGTGGGTTGCAAAACGTTCACGTACAGCGTCATAAACAGAGTCAACAACAACAACAGACTGTTCAGAAGCACAGATTACGCCGTTGTCGAAGGTTTTGGACATCAGTACAGATGCAACTGCACGTTTGATATCAGCAGTTTCATCGATAACAACTGGAGTGTTGCCCGCGCCTACACCGATAGCTGGTTTACCGGAGCTGTATGCGGCTT  >  NC_000913/1297242‑1297523

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A