Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F50 I2 R1	104	44.1	1376607	98.9%	1361464	150.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,499,529	A→G	G307G (GGT→GGC)	ydcK ←	uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,499,529	0	A	G	100.0%	92.7 / NA	27	G307G (GGT→GGC)	ydcK	uncharacterized protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (16/11); total (16/11)

GTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGTGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGAATCGGTCCGCCAC  >  NC_000913/1499383‑1499674

gTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGGCCa                                                                                                                                                >  2:466399/1‑150 (MQ=255)

    tATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTAACGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGGCCACGGAc                                                                                                                                           >  1:401740/1‑151 (MQ=255)

     aTCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGGCCACGGAc                                                                                                                                           >  2:502666/1‑150 (MQ=255)

      tCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGGCCACGGACAt                                                                                                                                         >  2:79462/1‑151 (MQ=255)

       ccTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGGCCACGGACAt                                                                                                                                         <  1:559158/150‑1 (MQ=255)

           gAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGGCCACGGACATGAAcc                                                                                                                                    >  2:638882/1‑151 (MQ=255)

               gTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGGCCACGGACATGAACCgtgt                                                                                                                                <  1:494345/151‑1 (MQ=255)

                   ccTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGGCCACGGACATGAACCGTGTCGcc                                                                                                                            >  2:11297/1‑151 (MQ=255)

                      gAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATCCGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGGCCACGGACATGAACCGTGTCGcta                                                                                                                           <  1:664751/149‑3 (MQ=255)

                           aGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGGCCACGGACATGAACCGTGTCGCCATCAAAgg                                                                                                                    <  2:601082/151‑1 (MQ=255)

                                gAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGGCCACGGACATGAACCGTGTCGCCATCAAAGGCTTc                                                                                                                <  2:31827/150‑1 (MQ=255)

                                  gTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGGCCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAac                                                                                                             >  1:350652/1‑151 (MQ=255)

                                    tCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGGCCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAacg                                                                                                            >  2:120789/1‑150 (MQ=255)

                                            gatgCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGGCCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGTgg                                                                                                   >  2:173988/1‑151 (MQ=255)

                                                     gatgatgTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGGCCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGTGGTCGGTTaa                                                                                           <  1:265442/150‑1 (MQ=255)

                                                        gatgTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGGCCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGTGGTCGGTTAACTcc                                                                                       >  2:281836/1‑151 (MQ=255)

                                                                                atCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGGCCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGTGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCa                                                               <  2:350663/151‑1 (MQ=255)

                                                                                   gATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGGCCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGTGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCt                                                            >  1:102410/1‑151 (MQ=255)

                                                                                      tCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGGCCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGTGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGt                                                          >  2:605970/1‑150 (MQ=255)

                                                                                       cacaATAACCCTGCCAGCGGCGTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGGCCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGTGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTa                                                         >  2:360954/1‑150 (MQ=255)

                                                                                              aCCCTGCCAGCGGCGTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGGCCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGTGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGct                                                 >  2:486808/1‑151 (MQ=255)

                                                                                                            gTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGGCCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGTGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGa                                   <  1:353796/151‑1 (MQ=255)

                                                                                                                            cctcGCCGTTAATGACTTTTGGGCCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGTGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAg                   <  1:134537/151‑1 (MQ=255)

                                                                                                                                   gTTAATGACTTTTGGGCCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGTGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGAATc            >  2:124443/1‑151 (MQ=255)

                                                                                                                                      aaTGACTTTTGGGCCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGTGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGAATCgg          <  2:531742/150‑1 (MQ=255)

                                                                                                                                      aaTGACTTTTGGGCCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGTGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGAATCGGt         <  1:61198/151‑1 (MQ=255)

                                                                                                                                             tttGGGCCACGGATATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGTGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGAATCGGTCCGCCAc  >  1:340465/1‑151 (MQ=255)

GTTTTATCCTTGAAGGTTGCCTGAAACAGGCTGAGTTCCTGAATGATGCCTATGATGATGTGAACTTATACGCGCGTATTATCGATTCACAATAACCCTGCCAGCGGCGTTCGCGTAATGCGCTCCTCGCCGTTAATGACTTTTGGACCACGGACATGAACCGTGTCGCCATCAAAGGCTTCAACGACGGCGTGGTCGGTTAACTCCACATGGTTTTCAATTATCACCGCACCTGTAATACGGCTCTCACCTTGTATGACAACGTGCTCGTCGAGCAGAATCGGTCCGCCAC  >  NC_000913/1499383‑1499674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A