Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F50 I2 R1
|
104 |
44.1 |
1376607 |
98.9% |
1361464 |
150.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,317,917 |
T→C |
T654A (ACC→GCC) |
rcsC ← |
hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,317,917 | 0 | T | C | 97.1%
| 108.8
/ ‑6.8
| 34 | T654A (ACC→GCC) | rcsC | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (11/22); minor base A (1/0); total (12/22) |
| Fisher's exact test for biased strand distribution p-value = 3.53e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01 |
TGCGGGTACTGAGCGCCGTACAACGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTAT > NC_000913/2317787‑2318061
|
tGCGGGTACTGAGCGCCGTACAACGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCCCAAAGCGCCAGACCCAGACCGTCCCCCTGGAAATTACGCt < 1:507486/148‑1 (MQ=255)
gCGGGTACTGAGCGCCGTACAACGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTAc < 1:37278/151‑1 (MQ=255)
cTGAGCGCCGTACAACGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGtt > 2:415572/1‑151 (MQ=255)
gcgcGGTACAACGGAATCCGCACGGTAAAGTGGCTGCCCATTCCCGGTTCTGAATTTACCGTGATATCGCCGTCCTTCATTCTGATCAGTTTTTCACAAATCGTGAGACCCAGACCGGCCCCCGGGAAATTGCGCTGTTCGCCGGtt < 1:1278/147‑1 (MQ=255)
cGTACAACGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTg < 1:64328/151‑1 (MQ=255)
gTACAACGGAATACACACTGCAAACTCGCTCTCCGGACCCGGATATGAATCTTCCGAGATATCGCCGTCGTTCATGCTGATCAGTTTGTCGCCAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCATTCTGAcca < 1:101120/149‑2 (MQ=255)
agcGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGgaaga < 1:379866/149‑1 (MQ=255)
aaCGGAATACGCACGGTAGACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGgaaga < 2:623724/151‑1 (MQ=255)
aCGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCACCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATc < 1:1232/150‑1 (MQ=255)
gCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCaaa < 2:266539/151‑1 (MQ=255)
gCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCActtc < 1:530579/150‑1 (MQ=255)
cATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCActtctg < 1:470770/149‑2 (MQ=255)
aTTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCg > 2:354161/1‑151 (MQ=255)
cGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGTCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGt > 2:228380/1‑151 (MQ=255)
cGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGt < 1:469782/151‑1 (MQ=255)
ggTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTGCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTa < 1:358953/151‑1 (MQ=255)
gAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATcc < 1:580814/148‑1 (MQ=255)
aCCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACg < 2:529355/147‑1 (MQ=255)
gagaTATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTAt < 1:313875/151‑1 (MQ=255)
gCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAaca < 2:272666/151‑1 (MQ=255)
gTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACgg > 2:678814/1‑151 (MQ=255)
tGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATagag > 2:407574/1‑149 (MQ=255)
gATCAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATa < 2:635436/150‑1 (MQ=255)
cAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATc < 1:69891/150‑1 (MQ=255)
cAGTTTTTCACAAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATc < 2:394158/150‑1 (MQ=255)
cacaAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCcgc > 1:12889/1‑151 (MQ=255)
acaAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCcgcg > 1:84304/1‑151 (MQ=255)
caAATCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCcgcgc > 1:567039/1‑151 (MQ=255)
aaaTCGCCAGACCCAGACCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCcgcgcg > 1:516472/1‑151 (MQ=255)
ccagacccagacCGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATg > 1:676911/1‑151 (MQ=255)
gacccagaccGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCCGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCa > 2:660874/1‑150 (MQ=255)
cccagaccGGCCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCaaa < 2:107297/150‑1 (MQ=255)
accGGACCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACtat > 2:413005/1‑150 (MQ=255)
ccGGCCCCCTGGAAATTACGCTGTCCGCCAGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACGTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACt < 1:646176/147‑1 (MQ=255)
|
TGCGGGTACTGAGCGCCGTACAACGGAATACGCACGGTAAACTGGCTGCCCATTCCCGGTTCTGAATCTACCGAGATATCGCCGTCCATCATGCTGATCAGTTTTTCACAAATCGCCAGACCCAGACCGGTCCCCTGGAAATTACGCTGTACGCCCGTTCCGACCTGGAAGAAGGGATCAAACAAGCGCACCACTTCTTTCGCCGGTATCCCCACGCCGGTATCGCGAACACGGATAGAGAGATAATCGCCATCCGCGCGAACATGCAAAACTAT > NC_000913/2317787‑2318061
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
