Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F50 I3 R1 143 109.6 3434427 98.5% 3382910 150.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 665,401 C→T V305I (GTC→ATC)  mrdB ← cell wall shape‑determining protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913665,4010CT99.0% 422.3 / ‑5.2 104V305I (GTC→ATC) mrdBcell wall shape‑determining protein
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (49/54);  total (49/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 108 total aligned reads displayed.

CGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGG  >  NC_000913/665254‑665546
                                                                                                                                                   |                                                                                                                                                 
cGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGAtgcg                                                                                                                                                >  1:200279/1‑151 (MQ=255)
 gAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGAtgcg                                                                                                                                                >  2:1191829/1‑150 (MQ=255)
  aaCCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGa                                                                                                                                               >  1:1021730/1‑150 (MQ=255)
          cATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAggtg                                                                                                                                      >  1:466207/1‑151 (MQ=255)
           aTCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAggtgg                                                                                                                                     >  1:1082222/1‑151 (MQ=255)
               gCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTg                                                                                                                                 <  1:1117888/151‑1 (MQ=255)
                cacaATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTg                                                                                                                                 <  1:1207547/150‑1 (MQ=255)
                 acaATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTgcg                                                                                                                               >  1:972645/1‑151 (MQ=255)
                    aTTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGctc                                                                                                                            <  1:100127/151‑1 (MQ=255)
                              aTCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTAt                                                                                                                  >  2:24914/1‑151 (MQ=255)
                              aTCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTAt                                                                                                                  <  2:1402258/151‑1 (MQ=255)
                                cctccATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATcc                                                                                                                >  2:740667/1‑151 (MQ=255)
                                   ccATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCca                                                                                                               <  1:665523/149‑1 (MQ=255)
                                     aTAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGc                                                                                                           >  2:853161/1‑151 (MQ=255)
                                     aTAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGc                                                                                                           >  2:1028084/1‑151 (MQ=255)
                                     aTAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGc                                                                                                           >  1:857587/1‑151 (MQ=255)
                                       aaCTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGccc                                                                                                         >  2:182335/1‑151 (MQ=255)
                                           gACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCcgcg                                                                                                      <  2:1300228/150‑1 (MQ=255)
                                           gACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCcgc                                                                                                       <  2:1114516/149‑1 (MQ=255)
                                           gACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCgcgc                                                                                                     <  1:537084/151‑1 (MQ=255)
                                            aCCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCgcgc                                                                                                     >  1:1128229/1‑150 (MQ=255)
                                             ccAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGccc                                                                                                         <  2:253889/145‑1 (MQ=255)
                                             ccAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGccc                                                                                                         >  1:253866/1‑145 (MQ=255)
                                             ccAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCAt                                                                                                   <  2:708828/151‑1 (MQ=255)
                                               aGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATg                                                                                                  >  1:804755/1‑150 (MQ=255)
                                                gTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGAt                                                                                                >  2:965761/1‑151 (MQ=255)
                                                gTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGAt                                                                                                >  2:1173137/1‑151 (MQ=255)
                                                  gggAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATca                                                                                              <  2:1247821/151‑1 (MQ=255)
                                                  gggAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATca                                                                                              <  1:142866/151‑1 (MQ=255)
                                                  gggAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATca                                                                                              <  1:1543612/151‑1 (MQ=255)
                                                  gggAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATc                                                                                               <  2:186634/150‑1 (MQ=255)
                                                  gggAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATc                                                                                               <  2:256016/150‑1 (MQ=255)
                                                  gggAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATc                                                                                               >  2:783501/1‑150 (MQ=255)
                                                  gggAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATc                                                                                               >  2:17960/1‑150 (MQ=255)
                                                      gCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATcagc                                                                                            >  1:1388381/1‑149 (MQ=255)
                                                      gCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATcagc                                                                                            >  1:1692279/1‑149 (MQ=255)
                                                       cGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGa                                                                                         <  2:577419/151‑1 (MQ=255)
                                                        ggAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGaa                                                                                        <  1:760166/151‑1 (MQ=255)
                                                        ggAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGa                                                                                         <  2:24645/150‑1 (MQ=255)
                                                        ggAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGa                                                                                         >  1:890270/1‑150 (MQ=255)
                                                          aaCCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATg                                                                                      >  2:922180/1‑151 (MQ=255)
                                                          aaCCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATg                                                                                      >  1:899772/1‑151 (MQ=255)
                                                             cccTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTag                                                                                   <  1:717758/151‑1 (MQ=255)
                                                               cTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTag                                                                                   >  1:469832/1‑149 (MQ=255)
                                                                 aCAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTagaga                                                                                <  1:1198949/150‑1 (MQ=255)
                                                                 aCAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTagaga                                                                                <  1:1180668/150‑1 (MQ=255)
                                                                   aaCCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAgagcg                                                                             >  2:267261/1‑151 (MQ=255)
                                                                     ccGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAgggcgag                                                                           <  2:455844/151‑1 (MQ=255)
                                                                     ccGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAgagcgag                                                                           <  2:1534955/151‑1 (MQ=255)
                                                                         cAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGcaga                                                                       <  2:82124/151‑1 (MQ=255)
                                                                          aGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGcagaa                                                                      <  1:765283/151‑1 (MQ=255)
                                                                            aaTACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGcagaatc                                                                    <  2:293423/151‑1 (MQ=255)
                                                                             aTACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGcagaatca                                                                   <  1:671652/151‑1 (MQ=255)
                                                                             aTACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGcagaatca                                                                   <  1:1675082/151‑1 (MQ=255)
                                                                               aCCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGcagaatcaga                                                                 <  2:338134/151‑1 (MQ=255)
                                                                                    ttACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGcc                                                            >  1:564854/1‑151 (MQ=255)
                                                                                       ccataccaATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCAc                                                         >  2:1692762/1‑151 (MQ=255)
                                                                                        cataccaATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACt                                                        >  2:146382/1‑151 (MQ=255)
                                                                                           accaATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAAt                                                     >  2:704187/1‑151 (MQ=255)
                                                                                            ccaATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATc                                                    <  2:1212986/151‑1 (MQ=255)
                                                                                             caATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATcc                                                   >  1:1618248/1‑151 (MQ=255)
                                                                                             caATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATcc                                                   >  1:73619/1‑151 (MQ=255)
                                                                                              aaTATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATccc                                                  >  2:389765/1‑151 (MQ=255)
                                                                                               atatTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATccc                                                  >  1:400397/1‑150 (MQ=255)
                                                                                                 atTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGc                                               >  2:911897/1‑151 (MQ=255)
                                                                                                   ttACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGct                                              <  1:1592482/150‑1 (MQ=255)
                                                                                                     aCGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGctct                                            <  1:409791/150‑1 (MQ=255)
                                                                                                       gAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGGGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTcc                                         >  2:6193/1‑151 (MQ=255)
                                                                                                        aaGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCg                                        <  2:973327/151‑1 (MQ=255)
                                                                                                        aaGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCg                                        <  2:1138283/151‑1 (MQ=255)
                                                                                                        aaGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCg                                        >  2:1185553/1‑151 (MQ=255)
                                                                                                         aGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGc                                       <  1:1590388/151‑1 (MQ=255)
                                                                                                         aGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGc                                       >  2:478784/1‑151 (MQ=255)
                                                                                                                aaCGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTAcc                                >  2:59753/1‑151 (MQ=255)
                                                                                                                 aCGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCCCTAATCCCAGCTCTTCCGCCAGTACcg                               <  1:160821/151‑1 (MQ=255)
                                                                                                                  cGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACcgc                              >  2:876640/1‑151 (MQ=255)
                                                                                                                  cGAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACcgc                              <  2:668639/151‑1 (MQ=255)
                                                                                                                   gAATAATATCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACcgcg                             >  1:103254/1‑151 (MQ=255)
                                                                                                                       aataTCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAg                          <  2:1684917/150‑1 (MQ=255)
                                                                                                                          atCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATa                       >  1:840830/1‑150 (MQ=255)
                                                                                                                           tCAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATa                       <  1:267237/149‑1 (MQ=255)
                                                                                                                            cAGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATaa                      >  1:1300318/1‑149 (MQ=255)
                                                                                                                             aGCATTAAGCCGCCAGCCATGATGCGCCCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATaa                      <  1:1688523/148‑1 (MQ=255)
                                                                                                                             aGCATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGt                   <  2:453257/151‑1 (MQ=255)
                                                                                                                               cATTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCa                 <  2:80742/151‑1 (MQ=255)
                                                                                                                                aTTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAg                <  2:691217/151‑1 (MQ=255)
                                                                                                                                aTTAAGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAg                <  2:1082307/151‑1 (MQ=255)
                                                                                                                                   aaGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTa              <  2:519181/150‑1 (MQ=255)
                                                                                                                                   aaGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTa              <  1:146374/150‑1 (MQ=255)
                                                                                                                                   aaGCCGCCAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTAt             >  1:1075707/1‑151 (MQ=255)
                                                                                                                                     gccgccAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATgg           >  1:91738/1‑151 (MQ=255)
                                                                                                                                     gccgccAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATg            <  1:1040372/150‑1 (MQ=255)
                                                                                                                                      ccgccAGCCATGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGc          >  1:268004/1‑151 (MQ=255)
                                                                                                                                       cgccAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCg         <  1:1507836/151‑1 (MQ=255)
                                                                                                                                          cagccaTGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTc      >  2:1201315/1‑151 (MQ=255)
                                                                                                                                           agccaTGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTc      <  2:466250/150‑1 (MQ=255)
                                                                                                                                             ccaTGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCggg   <  2:889344/151‑1 (MQ=255)
                                                                                                                                              caTGATGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCgggg  >  1:538466/1‑151 (MQ=255)
                                                                                                                                               atgatgCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTGGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCgggg  >  2:1114700/1‑150 (MQ=255)
                                                                                                                                               atgatgCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCgggg  >  2:925167/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CGAACCCAGCCATCAGCACAATTAGCGCCGATCCTCCATAACTGACCAGTGGGAGCGGAACCCCTACAACCGGCAGAATACCGCTTACCATACCAATATTTACGAAGACATAAACGAATAATATCAGCATTAAGCCGCCAGCCATGACGCGACCAAAGGTGGTTTGCGCTCTGGCGGCTATCCACAGCCCGCGCATGATCAGCAGAATGTAGAGAGCGAGCAGAATCAGAATGCCCACTAATCCCAGCTCTTCCGCCAGTACCGCGAAGATAAAGTCAGTATGGCGTTCGGGG  >  NC_000913/665254‑665546

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A