Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F50 I3 R1	143	109.6	3434427	98.5%	3382910	150.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,528,995	T→C	E394E (GAA→GAG)	ligA ←	DNA ligase, NAD(+)‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,528,995	0	T	C	98.9%	345.5 / ‑3.8	90	E394E (GAA→GAG)	ligA	DNA ligase, NAD(+)‑dependent
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (37/52); total (38/52)
Fisher's exact test for biased strand distribution p-value = 4.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

TTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGG  >  NC_000913/2528855‑2529139

ttCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGc                                                                                                                                         <  1:1171800/150‑1 (MQ=255)

ttCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGc                                                                                                                                         >  2:1278521/1‑150 (MQ=255)

 tCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCac                                                                                                                                       >  2:1674057/1‑151 (MQ=255)

 tCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCa                                                                                                                                        <  2:1001639/150‑1 (MQ=255)

    gCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCacgac                                                                                                                                    <  1:343172/151‑1 (MQ=255)

     cGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCacgacg                                                                                                                                   <  1:1120619/151‑1 (MQ=255)

     agACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCacgacg                                                                                                                                   >  2:970765/2‑151 (MQ=255)

      gACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGt                                                                                                                                  >  1:280538/1‑151 (MQ=255)

       aCTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGtt                                                                                                                                 >  1:1253305/1‑151 (MQ=255)

       aCTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGtt                                                                                                                                 <  1:533013/151‑1 (MQ=255)

         tctTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTa                                                                                                                                >  2:597306/1‑150 (MQ=255)

              aCGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAaccacc                                                                                                                          >  1:1436343/1‑151 (MQ=255)

               cGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAaccacc                                                                                                                          >  1:339004/1‑150 (MQ=255)

                gCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTg                                                                                                                        <  2:95013/151‑1 (MQ=255)

                     gcACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCggg                                                                                                                    >  2:1458086/1‑150 (MQ=255)

                     gcACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGATATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGa                                                                                                                   <  1:552131/151‑1 (MQ=255)

                            aaaTCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTc                                                                                                            <  2:1305008/151‑1 (MQ=255)

                            aaaTCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTc                                                                                                            <  1:687973/151‑1 (MQ=255)

                                cAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGcc                                                                                                         <  1:155218/150‑1 (MQ=255)

                                cAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCa                                                                                                        <  2:1321841/151‑1 (MQ=255)

                                 aGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGcc                                                                                                         >  2:1465756/1‑149 (MQ=255)

                                  ggCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGTGATCACGTCGCCAgc                                                                                                      <  1:168408/151‑1 (MQ=255)

                                   gCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAgcg                                                                                                     <  1:534206/151‑1 (MQ=255)

                                    ccACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAgcgc                                                                                                    >  2:847499/1‑151 (MQ=255)

                                     cACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGcgcg                                                                                                   <  1:1362320/151‑1 (MQ=255)

                                      aCCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCgcgg                                                                                                  >  1:892496/1‑151 (MQ=255)

                                         ggTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGa                                                                                               <  1:304061/151‑1 (MQ=255)

                                              aGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATcac                                                                                          >  1:94996/1‑151 (MQ=255)

                                              aGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATca                                                                                           >  1:347994/1‑150 (MQ=255)

                                                 gggCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCAGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATcaccac                                                                                       >  1:820666/1‑151 (MQ=255)

                                                    cAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACttt                                                                                    >  2:1390930/1‑151 (MQ=255)

                                                    cAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACttt                                                                                    >  1:1164081/1‑151 (MQ=255)

                                                     aaCCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTa                                                                                   <  1:160325/151‑1 (MQ=255)

                                                     aaCCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTa                                                                                   <  1:1312577/151‑1 (MQ=255)

                                                        cGCTTCCCCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCg                                                                                <  2:1209348/151‑1 (MQ=255)

                                                        cGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCg                                                                                <  2:178956/151‑1 (MQ=255)

                                                        cGCTTCACCTTCCACACGCCCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACACCCTCACGGGCATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCg                                                                                >  1:1373732/1‑151 (MQ=255)

                                                         gCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGc                                                                               <  1:237316/151‑1 (MQ=255)

                                                             cACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAAt                                                                           >  2:1257878/1‑151 (MQ=255)

                                                               ccTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGc                                                                         <  2:697076/151‑1 (MQ=255)

                                                                 ttCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGt                                                                       >  1:638415/1‑151 (MQ=255)

                                                                 ttCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGt                                                                       >  2:611491/1‑151 (MQ=255)

                                                                 atCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGt                                                                       >  2:1642878/2‑151 (MQ=255)

                                                                  tCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGt                                                                       <  1:912426/150‑1 (MQ=255)

                                                                   ccACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTaa                                                                     <  2:274600/151‑1 (MQ=255)

                                                                   ccACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTaa                                                                     <  2:274193/151‑1 (MQ=255)

                                                                   ccACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTa                                                                      >  2:1104487/1‑150 (MQ=255)

                                                                    cacaCGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTaaa                                                                    <  2:1308934/151‑1 (MQ=255)

                                                                       acGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCa                                                                 <  2:161898/151‑1 (MQ=255)

                                                                         gCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCaa                                                                <  2:892564/150‑1 (MQ=255)

                                                                         gCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCa                                                                 >  1:246155/1‑149 (MQ=255)

                                                                            cAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGa                                                              <  1:48720/149‑1 (MQ=255)

                                                                              aCGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGtt                                                          <  1:1000388/151‑1 (MQ=255)

                                                                              aCGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGtt                                                          <  1:657237/151‑1 (MQ=255)

                                                                                gTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCg                                                        <  2:1022343/151‑1 (MQ=255)

                                                                                   aGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGAtt                                                     <  1:769377/151‑1 (MQ=255)

                                                                                    gAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGAttt                                                    <  1:1465657/151‑1 (MQ=255)

                                                                                    gAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGAttt                                                    <  2:1211172/151‑1 (MQ=255)

                                                                                     aaCCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTc                                                   <  2:1619698/151‑1 (MQ=255)

                                                                                          caTACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCg                                              >  2:1127698/1‑151 (MQ=255)

                                                                                             aCCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCa                                            <  1:977676/150‑1 (MQ=255)

                                                                                              ccGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCAtt                                          <  2:1131715/151‑1 (MQ=255)

                                                                                               cGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATtg                                         <  1:1568023/151‑1 (MQ=255)

                                                                                                  aCAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCGCCGGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTgtgt                                      <  2:92769/151‑1 (MQ=255)

                                                                                                  aCAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTgtgt                                      <  1:1437981/151‑1 (MQ=255)

                                                                                                  aCAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTgtgt                                      >  2:1099695/1‑151 (MQ=255)

                                                                                                     aTGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAg                                   <  2:1677705/151‑1 (MQ=255)

                                                                                                     aTGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAg                                   <  1:658380/151‑1 (MQ=255)

                                                                                                       gCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAgg                                  <  1:1682349/150‑1 (MQ=255)

                                                                                                           cGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCg                             >  2:1162222/1‑151 (MQ=255)

                                                                                                            gggAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGt                            >  1:1533297/1‑151 (MQ=255)

                                                                                                             ggAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGtt                           >  1:60484/1‑151 (MQ=255)

                                                                                                              gAATACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTa                          <  1:504507/151‑1 (MQ=255)

                                                                                                               aaTACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTAc                         >  1:324787/1‑151 (MQ=255)

                                                                                                                aTACAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACt                        >  1:2427/1‑151 (MQ=255)

                                                                                                                  aCAACCTCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCa                      <  2:1012931/151‑1 (MQ=255)

                                                                                                                        tCACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGc                 >  1:87140/1‑150 (MQ=255)

                                                                                                                         cACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGc                 <  1:591525/149‑1 (MQ=255)

                                                                                                                         cACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGc                 <  1:597251/149‑1 (MQ=255)

                                                                                                                         cACGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCAc               <  1:1023215/151‑1 (MQ=255)

                                                                                                                           cGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGc             <  2:373935/151‑1 (MQ=255)

                                                                                                                           cGGGTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGc             >  2:258586/1‑151 (MQ=255)

                                                                                                                              gTATCTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTg          <  1:280386/151‑1 (MQ=255)

                                                                                                                                  cTTCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAAc      >  2:1135447/1‑151 (MQ=255)

                                                                                                                                    tCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCa        <  1:1278426/147‑1 (MQ=255)

                                                                                                                                    tCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAAc      <  1:828143/149‑1 (MQ=255)

                                                                                                                                    tCCGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACAt    >  2:1380361/1‑151 (MQ=255)

                                                                                                                                      cGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATgg  >  2:619421/1‑151 (MQ=255)

                                                                                                                                      cGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATgg  >  2:239275/1‑151 (MQ=255)

                                                                                                                                      cGGGCGCTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATgg  >  1:974331/1‑151 (MQ=255)

TTCAGCGACTCTTTACGCTGCGCACCGCAAATCAGGCCACCGGTACAGCGGGCAACCGCTTCACCTTCCACACGCTCAACGTCAGAACCACATACCGGACAATGCGTCGGGAATACAACCTCACGGGTATCTTCCGGGCGTTCAGAAAGCACGACGTTAACCACCTGCGGGATCACGTCGCCAGCGCGGCGAATCACCACTTTATCGCCAATGCGTAAACCAAGACGTTCGATTTCATCCGCATTGTGTAAGGTTGCGTTACTCACCAGCACGCCTGCAACATGG  >  NC_000913/2528855‑2529139

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A