Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A3 F50 I2 R1	193	39.9	1341687	91.8%	1231668	149.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,530,256	G→A	A326A (GCC→GCT)	zipA ←	FtsZ stabilizer

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,530,256	0	G	A	100.0%	104.2 / NA	27	A326A (GCC→GCT)	zipA	FtsZ stabilizer
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (15/12); total (15/12)

GATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTGCGCAGATTGCAGCATCAGCTTGAAGTTCTGCAGCT  >  NC_000913/2530110‑2530401

gATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCg                                                                                                                                                <  2:494570/150‑1 (MQ=255)

      gATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGa                                                                                                                                         <  1:400056/151‑1 (MQ=255)

         atTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACtt                                                                                                                                      >  1:259183/1‑151 (MQ=255)

          ttCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTc                                                                                                                                     >  2:641682/1‑151 (MQ=255)

           tCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTcg                                                                                                                                    >  2:398953/1‑151 (MQ=255)

                   gCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGgatgat                                                                                                                            <  2:321236/151‑1 (MQ=255)

                   gCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGgatgat                                                                                                                            <  2:248557/151‑1 (MQ=255)

                    cGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGatgatg                                                                                                                           >  1:538947/1‑151 (MQ=255)

                     gAAGCGTCGTTCGCAGTTCCGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGt                                                                                                                          >  2:199618/1‑151 (MQ=255)

                         cgtcgtTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTg                                                                                                                      >  1:642719/1‑151 (MQ=255)

                         cgtcgtTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTg                                                                                                                      <  2:133251/151‑1 (MQ=255)

                             gtTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTGGTAc                                                                                                                  >  1:548255/1‑151 (MQ=255)

                                                    ttCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGtcat                                                                                           >  2:231118/1‑151 (MQ=255)

                                                      cGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGtcatca                                                                                         <  1:60123/151‑1 (MQ=255)

                                                       gattgattCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTcatcat                                                                                        <  1:577976/151‑1 (MQ=255)

                                                                             cAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCAc                                                                   <  2:590207/150‑1 (MQ=255)

                                                                                    aaaaaCCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGc                                                           >  2:235333/1‑151 (MQ=255)

                                                                                     aaaaCCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGcc                                                          >  2:346778/1‑151 (MQ=255)

                                                                                     aaaaCCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGcc                                                          >  1:461308/1‑151 (MQ=255)

                                                                                                         gTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATAtgct                                      <  2:121586/151‑1 (MQ=255)

                                                                                                             gAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTgcgc                                  >  2:135163/1‑151 (MQ=255)

                                                                                                              aaGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTgcgc                                  >  1:157508/1‑150 (MQ=255)

                                                                                                                   gagTTAATTTGCCTTAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTGCGCAGATTg                            >  2:56581/1‑151 (MQ=255)

                                                                                                                                 tAAGTGTATCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTGCGCAGATTGCAGCATCAGCTTg               <  2:460589/150‑1 (MQ=255)

                                                                                                                                        aTCAGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGTGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTGCGCAGATTGCAGCATCAGCTTGAAGTTCt        <  1:49448/150‑1 (MQ=255)

                                                                                                                                           aGGCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTGCGCAGATTGCAGCATCAGCTTGAAGTTCTGc      >  2:261452/1‑149 (MQ=255)

                                                                                                                                             gCGTTAGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTGCGCAGATTGCAGCATCAGCTTGAAGTTCTGCAGCt  <  2:476005/151‑1 (MQ=255)

GATAAAGATATTCATGATGGCGAAGCGTCGTTCGCAGTTCTGTCAGTTGTTGTTCGATTGATTCCATATCGCACCATCAATGCTAAAAACCCCCGACAAGCGGGGGTTCGAAGAGGAGTTAATTTGCCTTAAGTGTATCAGGCGTTGGCGTCTTTGACTTCGCGGATGATGTCCTGGTACTCGCGCAATTTCTGCGGAGTCATCATACGGCGCTGATCGTCAAGCACGACACCGCCCACTTCATCGGCAATATGCTGCGCAGATTGCAGCATCAGCTTGAAGTTCTGCAGCT  >  NC_000913/2530110‑2530401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A