Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F50 I1 R1	141	76.7	2402765	98.4%	2364320	150.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,421,732	A→G	T9A (ACA→GCA)	yfcH →	putative NAD‑dependent nucleotide‑sugar epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,421,732	0	A	G	100.0%	200.2 / NA	53	T9A (ACA→GCA)	yfcH	putative NAD‑dependent nucleotide‑sugar epimerase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (27/26); total (27/26)

AACATCACTGGGTGACGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGACAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACC  >  NC_000913/2421590‑2421876

aaCGTCACTGGGTGACGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTa                                                                                                                                          <  1:25602/151‑1 (MQ=255)

 aCATCACTGGGTGACGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTaa                                                                                                                                         <  2:1178716/151‑1 (MQ=255)

  cATCACTGGGTGACGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAAt                                                                                                                                        <  1:1176256/151‑1 (MQ=255)

    tCACTGGGTGACGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTg                                                                                                                                      >  1:351222/1‑151 (MQ=255)

      aCTGGGTGACGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGt                                                                                                                                    >  1:347788/1‑151 (MQ=255)

         gggTGACGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTc                                                                                                                                   <  1:187778/149‑1 (MQ=255)

           gTGACGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGcc                                                                                                                                >  1:111854/1‑150 (MQ=255)

               cGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTg                                                                                                                           <  2:523450/151‑1 (MQ=255)

                gTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGa                                                                                                                          <  2:960949/151‑1 (MQ=255)

                  aTGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGAtt                                                                                                                        <  1:1025443/151‑1 (MQ=255)

                     cTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCcg                                                                                                                     >  2:792492/1‑151 (MQ=255)

                       gAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCcg                                                                                                                     <  1:225822/149‑1 (MQ=255)

                         aGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGtt                                                                                                                 <  1:622277/151‑1 (MQ=255)

                          gTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGttt                                                                                                                <  1:203879/151‑1 (MQ=255)

                          gTGGAGATCGATAAACTGCACGCGCTGAGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGttt                                                                                                                >  2:1058700/1‑151 (MQ=255)

                                aTCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGa                                                                                                          <  1:248771/151‑1 (MQ=255)

                                    aTAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTg                                                                                                      >  2:256040/1‑151 (MQ=255)

                                      aaaCTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTggg                                                                                                    >  2:605155/1‑151 (MQ=255)

                                         cTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGcc                                                                                                  >  1:662470/1‑150 (MQ=255)

                                                 gctgcgctACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTa                                                                                         <  1:861219/151‑1 (MQ=255)

                                                     cgctACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGt                                                                                     <  1:1105150/151‑1 (MQ=255)

                                                      gctACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTa                                                                                    <  1:344238/151‑1 (MQ=255)

                                                        tACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGt                                                                                  >  2:336651/1‑151 (MQ=255)

                                                        tACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGt                                                                                  >  1:164668/1‑151 (MQ=255)

                                                         aCGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGt                                                                                  <  2:828165/150‑1 (MQ=255)

                                                            ccGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGAcg                                                                              >  2:193062/1‑151 (MQ=255)

                                                             cGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGAcgc                                                                             <  1:1174400/151‑1 (MQ=255)

                                                                  cGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTaa                                                                         <  2:125997/150‑1 (MQ=255)

                                                                   ggTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAAcc                                                                       <  2:1054059/151‑1 (MQ=255)

                                                                     tATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCg                                                                     >  1:509883/1‑151 (MQ=255)

                                                                      aTCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGc                                                                    >  1:1163185/1‑151 (MQ=255)

                                                                          aTGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGaa                                                                <  2:843537/151‑1 (MQ=255)

                                                                                  aaGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGtt                                                        <  2:632651/151‑1 (MQ=255)

                                                                                             gTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGc                                             >  2:11263/1‑151 (MQ=255)

                                                                                                    gCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCggg                                      >  1:536779/1‑151 (MQ=255)

                                                                                                     ccATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGt                                     <  1:1043764/151‑1 (MQ=255)

                                                                                                           cGGGAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACt                               >  1:896418/1‑151 (MQ=255)

                                                                                                            gggAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACt                               >  1:844575/1‑150 (MQ=255)

                                                                                                             ggAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTAt                             >  2:247285/1‑151 (MQ=255)

                                                                                                              gAGGCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATg                            <  1:615961/151‑1 (MQ=255)

                                                                                                                ggCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATg                            >  2:110192/1‑149 (MQ=255)

                                                                                                                ggCTGCATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGc                          <  1:339790/151‑1 (MQ=255)

                                                                                                                     cATGAACATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAggg                     >  1:604288/1‑151 (MQ=255)

                                                                                                                           cATAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGcc               >  1:578009/1‑151 (MQ=255)

                                                                                                                             tAGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCg              >  1:596261/1‑150 (MQ=255)

                                                                                                                              aGTGATCACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGAt            <  2:111856/151‑1 (MQ=255)

                                                                                                                                    cACCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAg       <  1:1187024/150‑1 (MQ=255)

                                                                                                                                     aCCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGc      >  2:393460/1‑150 (MQ=255)

                                                                                                                                     aCCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGc      >  2:665749/1‑150 (MQ=255)

                                                                                                                                     aCCGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCa     <  2:876973/151‑1 (MQ=255)

                                                                                                                                       cGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAAc   >  2:372190/1‑151 (MQ=255)

                                                                                                                                       cGGAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAAc   >  2:341281/1‑151 (MQ=255)

                                                                                                                                        ggAGGGGCAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAAcc  >  1:1120035/1‑151 (MQ=255)

AACATCACTGGGTGACGTATGCTGAAGTGGAGATCGATAAACTGCACGCGCTGCGCTACGCCGATTCGGTATCCATGACCTTAAGCTGGCAGCGTTAATCGCCATATCGGGAGGCTGCATGAACATAGTGATCACCGGAGGGACAGGATTAATTGGTCGCCATTTGATTCCGCGTTTGCTGGAGCTGGGCCATCAAATTACGGTAGTGACGCGTAACCCGCAGAAAGCCAGTTCCGTTCTCGGCCCTCGGGTGACACTATGGCAAGGGCTTGCCGATCAAAGCAACC  >  NC_000913/2421590‑2421876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A