Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	586,073	C→T	pseudogene (17/45 nt)	pauD →	tRNA‑OTHER

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	586,073	0	C	T	100.0%	178.4 / NA	46	pseudogene (17/45 nt)	pauD	tRNA‑OTHER
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (22/24); total (22/24)

AAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCT  >  NC_000913/585926‑586211

aaaaGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGc                                                                                                                                          >  1:76123/1‑150 (MQ=255)

    gagGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGtt                                                                                                                                     >  1:198569/1‑151 (MQ=255)

    gagGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGtt                                                                                                                                     >  1:231174/1‑151 (MQ=255)

     agGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTc                                                                                                                                    <  2:67171/151‑1 (MQ=255)

           aaaaCTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATcc                                                                                                                              >  1:423848/1‑151 (MQ=255)

             aaCTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTg                                                                                                                            >  2:377897/1‑151 (MQ=255)

               cTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGc                                                                                                                           >  2:478658/1‑150 (MQ=255)

               cTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGc                                                                                                                           >  1:495193/1‑150 (MQ=255)

                   gaCACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGgc                                                                                                                      >  2:154235/1‑151 (MQ=255)

                   gaCACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGgc                                                                                                                      <  2:304318/151‑1 (MQ=255)

                         tAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGcc                                                                                                                 >  1:566230/1‑150 (MQ=255)

                           aGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCAtt                                                                                                              <  1:368316/151‑1 (MQ=255)

                            ggCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCAttt                                                                                                             <  2:479213/151‑1 (MQ=255)

                               cTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATttctt                                                                                                          >  2:515055/1‑151 (MQ=255)

                               cTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATttctt                                                                                                          >  1:49096/1‑151 (MQ=255)

                                  gCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCt                                                                                                       >  2:550815/1‑151 (MQ=255)

                                          ttGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATg                                                                                               >  1:613810/1‑151 (MQ=255)

                                           tGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTAt                                                                                                <  2:570535/149‑1 (MQ=255)

                                            gCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGcc                                                                                             <  1:262155/151‑1 (MQ=255)

                                            gCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGc                                                                                              <  2:38396/150‑1 (MQ=255)

                                            gCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGc                                                                                              <  2:317162/150‑1 (MQ=255)

                                               aGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGt                                                                                          >  2:579071/1‑151 (MQ=255)

                                                      aCATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATcc                                                                                   >  1:38011/1‑151 (MQ=255)

                                                        aTGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGt                                                                                 <  1:325512/151‑1 (MQ=255)

                                                               gAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCt                                                                          <  1:13083/151‑1 (MQ=255)

                                                                aGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCtt                                                                         >  1:310510/1‑151 (MQ=255)

                                                                       gggACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCtt                                                                  >  1:182960/1‑151 (MQ=255)

                                                                         gACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTca                                                                <  2:42665/151‑1 (MQ=255)

                                                                                 aGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCa                                                        <  1:484855/151‑1 (MQ=255)

                                                                                    gcAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACtt                                                     <  1:599349/151‑1 (MQ=255)

                                                                                     cAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACttt                                                    <  1:558677/151‑1 (MQ=255)

                                                                                        agaTGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTg                                                 <  2:627675/151‑1 (MQ=255)

                                                                                                   tAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTgg                                      >  1:371680/1‑151 (MQ=255)

                                                                                                       ttgttgACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCg                                    >  1:416790/1‑149 (MQ=255)

                                                                                                               ttAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTc                           >  2:622850/1‑150 (MQ=255)

                                                                                                                    aTGTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGgt                     >  1:81018/1‑151 (MQ=255)

                                                                                                                      gTCGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGgtgt                   <  1:310060/151‑1 (MQ=255)

                                                                                                                        cGTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTc                  <  2:190904/150‑1 (MQ=255)

                                                                                                                         gTTCTAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAg                <  1:629214/151‑1 (MQ=255)

                                                                                                                             tAGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCGGTCTTCACCGTTTCCGCTTTGCCCTTCACCACACCACTTTTTGCCGCGGTTTGGCGAGATAATTCAACGGTGCCAGGc              >  2:315902/1‑149 (MQ=38)

                                                                                                                              aGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGcc             <  1:550826/149‑1 (MQ=255)

                                                                                                                              aGGAACTTCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGc              <  1:545182/148‑1 (MQ=255)

                                                                                                                                     tCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAAc      <  2:263325/149‑1 (MQ=255)

                                                                                                                                     tCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACg     <  2:521049/150‑1 (MQ=255)

                                                                                                                                     tCTAAGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACg     <  1:252785/150‑1 (MQ=255)

                                                                                                                                        aaGTCGTGGGCTGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCt  <  2:611403/150‑1 (MQ=255)

AAAAGAGGGACAAAACTGAGACACATAAGGCCTCGCAATGGCTTGCAAGGCTTTACATGTTTTGAGGTAGTGGGACGTGTGAGCGCAGAGATGGCGCGGTAAGTTGTTGACTTAAAATGTCGTTCTAGGAACTTCTAAGTCGTGGGCCGCAGGTTCGAATCCTGCAGGGCGCGCCATTTCTTCCTCATTTATGCCCGTCTTATCCGTTTCCGCTTTGCCCTTCACCACATCACTTTTTGTCGCTGTTTGGCGAGATAATTCAACGGTGTCAGGCCGTAAAACGCCT  >  NC_000913/585926‑586211

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A