Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	642,536	A→G	N150S (AAT→AGT)	ybdR →	putative Zn‑dependent NAD(P)‑binding oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	642,536	0	A	G	100.0%	98.9 / NA	29	N150S (AAT→AGT)	ybdR	putative Zn‑dependent NAD(P)‑binding oxidoreductase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (13/16); total (13/16)

GCCGCCTGCGAAAATACCAATGCGGGTAAAGGCGCTGCGCTCAATAAAAAACAGATACCAGCTCCAGCGGCATTGTTTGGTTATAGTCACCTGTATGGCGGCGTTCCTGGTGGGCAGGCGGAATATGTCCGCGTCCCTAAAGGGAATGTGGGGCCGTTTAAAGTACCGCCTTTGCTTTCAGATGATAAAGCGCTTTTCCTTTCTGATATTCTGCCAACGGCATGGCAGGCAGCAAAAAATGCGCAGATCCAACAAGGTTCAAGCGTTGCAGTCTATGGTGCTGGTCCT  >  NC_000913/642391‑642678

gccgccTGCGAAAATACCAATGCGGGTAAAGGCGCTGCGCTCAATAAAAAACAGATACCAGCTCCAGCGGCATTGTTTGGTTATAGTCACCTGTATGGCGGCGTTCCTGGTGGGCAGGCGGAATATGTCCGCGTCCCTAAAGGGAGTGTgg                                                                                                                                           <  1:232956/151‑1 (MQ=255)

                      cGGGTAAAGGCGCTGCGCACAATAAAAAACAGATACCAGCTCCAGCGGCATTGTTTGGTTATAGTCACCTGTATGGCGGCGTTCCTGGTGGGCAGGCGGAATATGTCCGCGTCCCTAAAGGGAGTGTGGGGCCGTTTAAAGTACCGCCttt                                                                                                                     >  2:617312/1‑151 (MQ=255)

                           aaaGGCGCTGCGCTCAATAAAAAACAGATACCAGCTCCAGCGGCATTGTTTGGTTATAGTCACCTGTATGGCGGCGTTCCTGGTGGGCAGGCGGAATATGTCCGCGTCCCTAAAGGGAGTGTGGGGCCGTTTAAAGTACCGCCTTTGCtt                                                                                                                 <  1:577751/150‑1 (MQ=255)

                                    gcgctCAATAAAAAACAGATACCAGCTCCAGCGGCATTGTTTGGTTATAGTCACCTGTATGGCGGCGTTCCTGGTGGGCAGGCGGAATATGTCCGCGTCCCTAAAGGGAGTGTGGGGCCGTTTAAAGTACCGCCTTTGCTTTCAgatgat                                                                                                        >  2:77789/1‑150 (MQ=255)

                                      gctCAATAAAAAACAGATACCAGCTCCAGCGGCATTGTTTGGTTATAGTCACCTGTATGGCGGCGTTCCTGGTGGGCAGGCGGAATATGTCCGCGTCCCTAAAGGGAGTGTGGGGCCGTTTAAAGTACCGCCTTTGCTTTCAGATGATaaa                                                                                                     <  2:415615/151‑1 (MQ=255)

                                         cAATAAAAAACAGATACCAGCTCCAGCGGCATTGTTTGGTTATAGTCACCTGTATGGCGGCGTTCCTGGTGGGCAGGCGGAATATGTCCGCGTCCCTAAAGGGAGTGTGGGGCCGTTTAAAGTACCGCCTTTGCTTTCAGATGATAAAg                                                                                                    <  2:135103/149‑1 (MQ=255)

                                             aaaaaaCAGATACCAGCTCCAGCGGCATTGTTTGGTTATAGTCACCTGTATGGCGGCGTTCCTGGTGGGCAGGCGGAATATGTCCGCGTCCCTAAAGGGAGTGTGGGGCCGTTTAAAGTACCGCCTTTGCTTTCAGATGATAAAGCGCttt                                                                                              <  1:230215/151‑1 (MQ=255)

                                              aaaaaCAGATACCAGCTCCAGCGGCATTGTTTGGTTATAGTCACCTGTATGGCGGCGTTCCTGGTGGGCAGGCGGAATATGTCCGCGTCCCTAAAGGGAGTGTGGGGCCGTTTAAAGTACCGCCTTTGCTTTCAGATGATAAAGCGCtttt                                                                                             >  1:88933/1‑151 (MQ=255)

                                                   cAGATACCAGCTCCAGCGGCATTGTTTGGTTATAGTCACCTGTATGGCGGCGTTCCTGGTGGGCAGGCGGAATATGTCCGCGTCCCTAAAGGGAGTGTGGGGCCGTTTAAAGTACCGCCTTTGCTTTCAGATGATAAAGCGCTTTTCCttt                                                                                        >  1:421688/1‑151 (MQ=255)

                                                      aTACCAGCTCCAGCGGCATTGTTTGGTTATAGTCACCTGTATGGCGGCGTTCCTGGTGGGCAGGCGGAATATGTCCGCGTCCCTAAAGGGAGTGTGGGGCCGTTTAAAGTACCGCCTTTGCTTTCAGATGATAAAGCGCTTTTCCTTTCTg                                                                                     <  2:180323/151‑1 (MQ=255)

                                                       tACCAGCTCCAGCGGCATTGTTTGGTTATAGTCACCTGTATGGCGGCGTTCCTGGTGGGCAGGCGGAATATGTCCGCGTCCCTAAAGGGAGTGTGGGGCCGTTTAAAGTACCGCCTTTGCTTTCAGATGATAAAGCGCTTTTCCTTTCTg                                                                                     >  2:265499/1‑150 (MQ=255)

                                                        aCCAGCTCCAGCGGCATTGTTTGGTTATAGTCACCTGTATGGCGGCGTTCCTGGTGGGCAGGCGGAATATGTCCGCGTCCCTAAAGGGAGTGTGGGGCCGTTTAAAGTACCGCCTTTGCTTTCAGATGATAAAGCGCTTTTCCTTTCTGat                                                                                   >  2:415969/1‑151 (MQ=255)

                                                                     gCATTGTTTGGTTATAGTCACCTGTATGGCGGCGTTCCTGGTGGGCAGGCGGAATATGTCCGCGTCCCTAAAGGGAGTGTGGGGCCGTTTAAAGTACCGCCTTTGCTTTCAGATGATAAAGCGCTTTTCCTTTCTGATATTCTGCCAACgg                                                                      >  2:49080/1‑151 (MQ=255)

                                                                              ggTTATAGTCACCTGTATGGCGGCGTTCCTGGTGGGCAGGCGGAATATGTCCGCGTCCCTAAAGGGAGTGTGGGGCCGTTTAAAGTACCGCCTTTGCTTTCAGATGATAAAGCGCTTTTCCTTTCTGATATTCTGCCAACGGCATggcagg                                                             <  2:47237/151‑1 (MQ=255)

                                                                              ggTTATAGTCACCTGTATGGCGGCGTTCCTGGTGGGCAGGCGGAATATGTCCGCGTCCCTAAAGGGAGTGTGGGGCCGTTTAAAGTACCGCCTTTGCTTTCAGATGATAAAGCGCTTTTCCTTTCTGATATTCTGCCAACGGCATggcag                                                              <  1:173933/150‑1 (MQ=255)

                                                                                 tataGTCACCTGTATGGCGGCGTTCCTGGTGGGCAGGCGGAATATGTCCGCGTCCCTAAAGGGAGTGTGGGGCCGTTTAAAGTACCGCCTTTGCTTTCAGATGATAAAGCGCTTTTCCTTTCTGATATTCTGCCAACGGCATggcaggc                                                            >  1:236118/1‑149 (MQ=255)

                                                                                    aGTCACCTGTATGGCGGCGTTCCTGGTGGGCAGGCGGAATATGTCCGCGTCCCTAAAGGGAGTGTGGGGCCGTTTAAAGTACCGCCTTTGCTTTCAGATGATAAAGCGCTTTTCCTTTCTGATATTCTGCCAACGGCATGGCAGgcagca                                                        <  1:370532/150‑1 (MQ=255)

                                                                                     gTCACCTGTATGGCGGCGTTCCTGGTGGGCAGGCGGAATATGTCCGCGTCCCTAAAGGGAGTGTGGGGCCGTTTAAAGTACCGCCTTTGCTTTCAGATGATAAAGCGCTTTTCCTTTCTGATATTCTGCCAACGGCATGGCAGGCAGCaaa                                                      <  2:222063/151‑1 (MQ=255)

                                                                                        aCCTGTATGGCGGCGTTCCTGGTGGGCAGGCGGAATATGTCCGCGTCCCTAAAGGGAGTGTGGGGCCGTTTAAAGTACCGCCTTTGCTTTCAGATGATAAAGCGCTTTTCCTTTCTGATATTCTGCCAACGGCATGGCAGGCAGCaaaaa                                                    >  1:377370/1‑150 (MQ=255)

                                                                                         ccTGTATGGCGGCGTTCCTGGTGGGCAGGCGGAATATGTCCGCGTCCCTAAAGGGAGTGTGGGGCCGTTTAAAGTACCGCCTTTGCTTTCAGATGATAAAGCGCTTTTCCTTTCTGATATTCTGCCAACGGCATGGCAGGCAGCaaaaaa                                                   >  2:233204/1‑150 (MQ=255)

                                                                                                ggcggcGTTCCTGGTGGGCAGGCGGAATATGTCCGCGTCCCTAAAGGGAGTGTGGGGCCGTTTAAAGTACCGCCTTTGCTTTCAGATGATAAAGCGCTTTTCCTTTCTGATATTCTGCCAACGGCATGGCAGGCAGCAAAAAATGCGCAGa                                           <  2:612606/151‑1 (MQ=255)

                                                                                                     cgTTCCTGGTGGGCAGGCGGAATATGTCCGCGTCCCTAAAGGGAGTGTGGGGCCGTTTAAAGTACCGCCTTTGCTTTCAGATGATAAAGCGCTTTTCCTTTCTGATATTCTGCCAACGGCATGGCAGGCAGCAAAAAATGCGCAGATCcaa                                      <  2:74634/151‑1 (MQ=255)

                                                                                                      gTTCCTGGTGGGCAGGCGGAATATGTCCGCGTCCCTAAAGGGAGTGTGGGGCCGTTTAAAGTACCGCCTTTGCTTTCAGATGATAAAGCGCTTTTCCTTTCTGATATTCTGCCAACGGCATGGCAGGCAGCAAAAAATGCGCAGATCcaac                                     <  1:457604/151‑1 (MQ=255)

                                                                                                                              gTCCGCGTCCCTAAAGGGAGTGTGGGGCCGTTTAAAGTACCGCCTTTGCTTTCAGATGATAAAGCGCTTTTCCTTTCTGATATTCTGCCAACGGCATGGCAGGCAGCAAAAAATGCGCAGATCCAACAAGGTTCAAGCGTTGCAGTCTATg             >  1:299015/1‑151 (MQ=255)

                                                                                                                                ccGCGTCCCTAAAGGGAGTGTGGGGCCGTTTAAAGTACCGCCTTTGCTTTCAGATGATAAAGCGCTTTTCCTTTCTGATATTCTGCCAACGGCATGGCAGGCAGCAAAAAATGCGCAGATCCAACAAGGTTCAAGCGTTGCAGTCTATGGt           <  1:106156/151‑1 (MQ=255)

                                                                                                                                   cgTCCCTAAAGGGAGTGTGGGGCCGTTTAAAGTACCGCCTTTGCTTTCAGATGATAAAGCGCTTTTCCTTTCTGATATTCTGCCAACGGCATGGCAGGCAGCAAAAAATGCGCAGATCCAACAAGGTTCAAGCGTTGCAGTCTATGGTGCt        >  2:315627/1‑151 (MQ=255)

                                                                                                                                       ccTAAAGGGAGTGTGGGGCCGTTTAAAGTACCGCCTTTGCTTTCAGATGATAAAGCGCTTTTCCTTTCTGATATTCTGCCAACGGCATGGCAGGCAGCAAAAAATGCGCAGATCCAACAAGGTTCAAGCGTTGCAGTCTATGGTGCTGGTc    <  1:159631/151‑1 (MQ=255)

                                                                                                                                         tAAAGGGAGTGTGGGGCCGTTTAAAGTACCGCCTTTGCTTTCAGATGATAAAGCGCTTTTCCTTTCTGATATTCTGCCAACGGCATGGCAGGCAGCAAAAAATGCGCAGATCCAACAAGGTTCAAGCGTTGCAGTCTATGGTGCTGGTCCt  >  2:257887/1‑151 (MQ=255)

                                                                                                                                          aaaGGGAGTGTGGGGCCGTTTAAAGTACCGCCTTTGCTTTCAGATGATAAAGCGCTTTTCCTTTCTGATATTCTGCCAACGGCATGGCAGGCAGCAAAAAATGCGCAGATCCAACAAGGTTCAAGCGTTGCAGTCTATGGTGCTGGTcc   <  2:696/149‑1 (MQ=255)

GCCGCCTGCGAAAATACCAATGCGGGTAAAGGCGCTGCGCTCAATAAAAAACAGATACCAGCTCCAGCGGCATTGTTTGGTTATAGTCACCTGTATGGCGGCGTTCCTGGTGGGCAGGCGGAATATGTCCGCGTCCCTAAAGGGAATGTGGGGCCGTTTAAAGTACCGCCTTTGCTTTCAGATGATAAAGCGCTTTTCCTTTCTGATATTCTGCCAACGGCATGGCAGGCAGCAAAAAATGCGCAGATCCAACAAGGTTCAAGCGTTGCAGTCTATGGTGCTGGTCCT  >  NC_000913/642391‑642678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A