Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	772,049	T→C	F198L (TTC→CTC)	cydA →	cytochrome d terminal oxidase, subunit I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	772,049	0	T	C	100.0%	145.4 / NA	39	F198L (TTC→CTC)	cydA	cytochrome d terminal oxidase, subunit I
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (21/18); total (21/18)

GGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGTTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAGGTCGTGACTTCGCCTTCGCTAAACGCTCCTTTGCTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGA  >  NC_000913/771902‑772188

ggCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGCTc                                                                                                                                           >  2:347863/1‑150 (MQ=255)

   tGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGCTCATcc                                                                                                                                       >  2:308874/1‑151 (MQ=255)

      aTGCAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGCTCATCCTCg                                                                                                                                    >  1:134557/1‑151 (MQ=255)

         cAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGCTCATCCTCGGTa                                                                                                                                 <  1:511314/151‑1 (MQ=255)

           aaaCCCAATCGCGTCCGATTTCAACTTTGAAACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGCTCATCCTCGGTATc                                                                                                                               <  1:136569/151‑1 (MQ=255)

            aaCCCAATCGCGTCCGATTTCAACTTTGAAACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGCTCATCCTCGGTATCa                                                                                                                              <  2:531684/151‑1 (MQ=255)

                 aaTCGCGTCCGATTTCAACTTTGAAACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGCTCATCCTCGGTATCAgcgc                                                                                                                          >  1:44704/1‑150 (MQ=255)

                         ccGATTTCAACTTTGAAACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGCTCATCCTCGGTATCAGCGCATGGTatat                                                                                                                 >  2:299033/1‑151 (MQ=255)

                          cGATTTCAACTTTGAAACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGCTCATCCTCGGTATCAGCGCATGGTATATg                                                                                                                <  1:345496/151‑1 (MQ=255)

                                 aaCTTTGAAACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGCTCATCCTCGGTATCAGCGCATGGTATATGCTGaa                                                                                                           >  1:97252/1‑149 (MQ=255)

                                       gAAACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGCTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAGGTCGt                                                                                                    <  1:158583/150‑1 (MQ=255)

                                       gAAACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGCTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAGGTCGTg                                                                                                   <  2:69001/151‑1 (MQ=255)

                                         aaCTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGCTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAGGTCGTGAc                                                                                                 <  1:28892/151‑1 (MQ=255)

                                             aTGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGCTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAGGTCGTGActtcg                                                                                             <  2:176257/151‑1 (MQ=255)

                                                 gTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGCTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAGGTCGTGActtcgcctt                                                                                         >  1:213543/1‑151 (MQ=255)

                                                        gaTGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGCTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAGGTCGTGACTTCGCCTTCGCTaa                                                                                   >  2:95239/1‑150 (MQ=255)

                                                                gCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGCTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAGGTCGTGACTTCGCCTTCGCTAAACGCTCCtt                                                                          <  2:431355/151‑1 (MQ=255)

                                                                  ttCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGCTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAGGTCGTGACTTCGCCTTCGCTAAACGCTCCTTTg                                                                        >  1:492166/1‑151 (MQ=255)

                                                                    ctcCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGCTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAGGCCGTGACTTCGCCTTCGCTAAACGCTCCTTTGc                                                                       >  2:538170/1‑150 (MQ=255)

                                                                      ccGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGCTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAGGTCGTGACTTCGCCTTCGCTAAACGCTCCTTTGCt                                                                      <  2:356021/149‑1 (MQ=255)

                                                                            tGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGCTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAGGTCGTGACTTCGCCTTCGCTAAACGCTCCTTTGCTATCGCTGc                                                              >  2:382115/1‑151 (MQ=255)

                                                                                      cccGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGCTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAGGTCGTGACTTCGCCTTCGCTAAACGCTCCTTTGCTATCGCTGCCAGCTTCgg                                                     >  2:306322/1‑150 (MQ=255)

                                                                                      cccGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGCTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAGGTCGTGACTTCGCCTTCGCTAAACGCTCCTTTGCTATCGCTGCCAGCTTCGGt                                                    <  2:573506/151‑1 (MQ=255)

                                                                                        cGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGCTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAGGTCGTGACTTCGCCTTCGCTAAACGCTCCTTTGCTATCGCTGCCAGCTTCGGTa                                                   >  1:108100/1‑150 (MQ=255)

                                                                                         ggTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGCTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAGGTCGTGACTTCGCCTTCGCTAAACGCTCCTTTGCTATCGCTGCCAGCTTCGGTATg                                                 >  2:364439/1‑151 (MQ=255)

                                                                                                cAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGCTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAGGTCGTGACTTCGCCTTCGCTAAACGCTCCTTTGCTATCGCTGCCAGCTTCGGTATGgctgct                                           <  2:492156/150‑1 (MQ=255)

                                                                                                         ttCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGCTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAGGTCGTGACTTCGCCTTCGCTAAACGCTCCTTTGCTATCGCTGCCAGCTTCGGTATGGCTGCTGTtctgtctg                                 >  1:109439/1‑151 (MQ=255)

                                                                                                          gcGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGCTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAGGTCGTGACTTCGCCTTCGCTAAACGCTCCTTTGCTATCGCTGCCAGCTTCGGTATGGCTGCTGTTctgtctgt                                >  2:455395/2‑151 (MQ=255)

                                                                                                                     gTAGCGTCTGGTTATGTGACTGGCGCGATGCTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAGGTCGTGACTTCGCCTTCGCTAAACGCTCCTTTGCTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCt                       <  2:409397/149‑1 (MQ=255)

                                                                                                                       aGCGTCTGGTTATGTGACTGGCGCGATGCTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAGGTCGTGACTTCGCCTTCGCTAAACGCTCCTTTGCTATCGCTGCCAGCTTCGGTATg                                                 <  1:594846/121‑1 (MQ=255)

                                                                                                                       aGCGTCTGGTTATGTGACTGGCGCGATGCTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAGGTCGTGACTTCGCCTTCGCTAAACGCTCCTTTGCTATCGCTGCCAGCTTCGGTATg                                                 >  2:594831/1‑121 (MQ=255)

                                                                                                                          gTCTGGTTATGTGACTGGCGCGATGCTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAGGTCGTGACTTCGCCTTCGCTAAACGCTCCTTTGCTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGtt                         <  2:256356/142‑1 (MQ=255)

                                                                                                                          gTCTGGTTATGTGACTGGCGCGATGCTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAGGTCGTGACTTCGCCTTCGCTAAACGCTCCTTTGCTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGtt                         >  1:256363/1‑142 (MQ=255)

                                                                                                                              ggTTATGTGACTGGCGCGATGCTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAGGTCGTGACTTCGCCTTCGCTAAACGCTCCTTTGCTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGtgatga              <  2:566292/149‑1 (MQ=255)

                                                                                                                                 tATGTGACTGGCGCGATGCTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAGGTCGTGACTTCGCCTTCGCTAAACGCTCCTTTGCTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATcc          <  1:336149/150‑1 (MQ=255)

                                                                                                                                 tATGTGACTGGCGCGATGCTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAGGTCGTGACTTCGCCTTCGCTAAACGCTCCTTTGCTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCg         >  1:70299/1‑151 (MQ=255)

                                                                                                                                  aTGTGACTGGCGCGATGCTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAGGTCGTGACTTCGCCTTCGCTAAACGCTCCTTTGCTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCgg        <  1:45647/151‑1 (MQ=255)

                                                                                                                                      gACTGGCGCGATGCTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAGGTCGTGACTTCGCCTTCGCTAAACGCTCCTTTGCTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGGATCCGGCTAc    >  2:307667/1‑151 (MQ=255)

                                                                                                                                           gcgcGATGCTCATCCTCGGAATCAGCGCATGGTATATGCTGCAAGGTCGTGACTTCGCCTTCGCTAAACGCTCCTTTGCTGTCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGa  >  2:10081/1‑148 (MQ=255)

GGCTGGATGCAAAACCCAATCGCGTCCGATTTCAACTTTGAAACTATGCGTATGGAGATGGTGAGCTTCTCCGAGCTGGTGCTTAACCCGGTTGCTCAGGTGAAATTCGTTCACACTGTAGCGTCTGGTTATGTGACTGGCGCGATGTTCATCCTCGGTATCAGCGCATGGTATATGCTGAAAGGTCGTGACTTCGCCTTCGCTAAACGCTCCTTTGCTATCGCTGCCAGCTTCGGTATGGCTGCTGTTCTGTCTGTTATTGTTCTGGGTGATGAATCCGGCTACGA  >  NC_000913/771902‑772188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A