Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	797,155	T→C	V201A (GTA→GCA)	modC →	molybdate ABC transporter ATPase; chlorate resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	797,155	0	T	C	93.5%	99.8 / ‑1.0	31	V201A (GTA→GCA)	modC	molybdate ABC transporter ATPase; chlorate resistance protein
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (13/16); total (14/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

GCTGGCGTCACTGGATATTCCGCGTAAACGCGAACTGTTGCCTTATCTGCAACGGCTGACACGGGAAATCAACATTCCGATGTTGTATGTCAGCCATTCGCTGGATGAGATCCTCCATCTGGCAGACAGAGTGATGGTACTGGAAAACGGTCAGGTGAAAGCCTTTGGCGCGCTGGAGGAAGTGTGGGGCAGTAGCGTGATGAATCCGTGGCTGCCGAAAGAGCAACAAAGTAGCATTCTGAAAGTGACGGTGCTTGAGCATCATCCGCATTACGCGA  >  NC_000913/797018‑797295

gCTGGCGTCACTGGATATTCCGCGTAAACGCGAACTGTTGCCTTATCTGCAACGGCTGACACGGGAAATCAACATTCCGATGTTGTATGTCAGCCATTCGCTGGATGAGATCCTCCATCTGGCAGACAGAGTGATGGCACTGGAAAACGGt                                                                                                                                 >  1:5700/1‑151 (MQ=255)

   ggCGTCACTGGATATTCCGCGTAAACGCGAACTGTTGCCTTATCTGCAACGGCTGACACGGGAAATCAACATTCCGATGTTGTATGTCAGCCATTCGCTGGATGAGATCCTCCATCTGGCAGACAGAGTGATGGCACTGGAAAACGGTc                                                                                                                                >  2:510505/1‑149 (MQ=255)

      gTCACTGGATATTCCGCGTAAACGCGAACTGTTGCCTTATCTGCAACGGCTGACACGGGAAATCAACATTCCGATGTTGTATGTCAGCCATTCGCTGGATGAGATCCTCCATCTGGCAGACAGAGTGATGGCACTGGAAAACGGTCAGGTg                                                                                                                           <  2:3150/151‑1 (MQ=255)

      gTCACTGGATATTCCGCGTAAACGCGAACTGTTGCCTTATCTGCAACGGCTGACACGGGAAATCAACATTCCGATGTTGTATGTCAGCCATTCGCTGGATGAGATCCTCCATCTGGCAGACAGAGTGATGGCACTGGAAAACGGTCAGGTg                                                                                                                           >  1:280399/1‑151 (MQ=255)

            ggATATTCCGCGTAAACGCGAACTGTTGCCTTATCTGCAACGGCTGACACGGGAAATCAACATTCCGATGTTGTATGTCAGCCATTCGCTGGATGAGATCCTCCATCTGGCAGACAGAGTGATGGCACTGGAAAACGGTCAGGTGAAAGcc                                                                                                                     >  2:357398/1‑151 (MQ=255)

                atTCCGCGTAAACGCGAACTGTTGCCTTATCTGCAACGGCTGACACGGGAAATCAACATTCCGATGTTGTATGTCAGCCATTCGCTGGATGAGATCCTCCATCTGGCAGACAGAGTGATGGTACTGGAAAACGGTCAGGTGAAAGCCttt                                                                                                                  <  2:23508/150‑1 (MQ=255)

                           aCGCGAACTGTTGCCTTATCTGCAACGGCTGACACGGGAAATCAACATTCCGATGTTGTATGTCAGCCATTCGCTGGATGAGATCCTCCATCTGGCAGACAGAGTGATGGCACTGGAAAACGGTCAGGTGAAAGCCTTTGGCGCGCTgg                                                                                                        >  1:588762/1‑149 (MQ=255)

                             gcgAACTGTTGCCTTATCTGCAACGGCTGACACGGGAAATCAACATTCCGATGTTGTATGTCAGCCATTCGCTGGATGAGATCCTCCATCTGGCAGACAGAGTGATGGCACTGGAAAACGGTCAGGTGAAAGCCTTTGGCGCGCTggagg                                                                                                     >  1:132684/1‑150 (MQ=255)

                               gAACTGTTGCCTTATCTGCAACGGCTGACACGGGAAATCAACATTCCGATGTTGTATGTCAGCCATTCGCTGGATGAGATCCTCCATCTGGCAGACAGAGTGATGGCACTGGAAAACGGTCAGGTGAAAGCCTTTGGCGCGCTGGAGGAAg                                                                                                  <  2:379090/151‑1 (MQ=255)

                                aaCTGTTGCCTTATCTGCAACGGCTGACACGGGAAATCAACATTCCGATGTTGTATGTCAGCCATTCGCTGGATGAGATCCTCCATCTGGCAGACAGAGTGATGGCACTGGAAAACGGTCAGGTGAAAGCCTTTGGCGCGCTGGAGGAAgt                                                                                                 >  2:97439/1‑151 (MQ=255)

                                           tATCTGCAACGGCTGACACGGGAAATCAACATTCCGATGTTGTATGTCAGCCATTCGCTGGATGAGATCCTCCATCTGGCAGACAGAGTGATGGCACTGGAAAACGGTCAGGTGAAAGCCTTTGGCGCGCTGGAGGAAGTGTGGGGCAGt                                                                                       <  1:481105/150‑1 (MQ=255)

                                            aTCTGCAACGGCTGACACGGGAAATCAACATTCCGATGTTGTATGTCAGCCATTCGCTGGATGAGATCCTCCATCTGGCAGACAGAGTGATGGCACTGGAAAACGGTCAGGTGAAAGCCTTTGGCGCGCTGGAGGAAGTGTGGGGCAGTa                                                                                      <  2:294352/150‑1 (MQ=255)

                                                         gACACGGGAAATCAACATTCCGATGTTGTATGTCAGCCATTCGCTGGATGAGATCCTCCATCTGGCAGACAGAGTGATGGCACTGGAAAACGGTCAGGTGAAAGCCTTTGGCGCGCTGGAGGAAGTGTGGGGCAGTAGCGTGATGAATcc                                                                         <  1:452314/150‑1 (MQ=255)

                                                          acacGGGAAATCAACATTCCGATGTTGTATGTCAGCCATTCGCTGGATGAGATCCTCCATCTGGCAGACAGAGTGATGGCACTGGAAAACGGTCAGGTGAAAGCCTTTGGCGCGCTGGAGGAAGTGTGGGGCAGTAGCGTGATGAATCCGt                                                                       >  2:445119/1‑151 (MQ=255)

                                                           cacGGGAAATCAACATTCCGATGTTGTATGTCAGCCATTCGCTGGATGAGATCCTCCATCTGGCAGACAGAGTGATGGCACTGGAAAACGGTCAGGTGAAAGCCTTTGGCGCGCTGGAGGAAGTGTGGGGCAGTAGCGTGATGAATCCGTg                                                                      <  1:217124/151‑1 (MQ=255)

                                                              gggAAATCAACATTCCGATGTTGTATGTCAGCCATTCGCTGGATGAGATCCTCCATCTGGCAGACAGAGTGATGGCACTGGAAAACGGTCAGGTGAAAGCCTTTGGCGCGCTGGAGGAAGTGTGGGGCAGTAGCGTGATGAATCCGTGGCt                                                                   <  1:97439/151‑1 (MQ=255)

                                                              gggAAATCAACATTCCGATGTTGTATGTCAGCCATTCGCTGGATGAGATCCTCCATCTGGCAGACAGAGTGATGGCACTGGAAAACGGTCAGGTGAAAGCCTTTGGCGCGCTGGAGGAAGTGTGGGGCAGTAGCGTGATGAATCCGTGGCt                                                                   >  1:387623/1‑151 (MQ=255)

                                                                   aTCAACATTCCGATGTTGTATGTCAGCCATTCGCTGGATGAGATCCTCCATCTGGCAGACAGAGTGATGGCACTGGAAAACGGTCAGGTGAAAGCCTTTGGCGCGCTGGAGGAAGTGTGGGGCAGTAGCGTGATGAATCCGTGGCTGCCGa                                                              <  1:183195/151‑1 (MQ=255)

                                                                        cATTCCGATGTTGTATGTCAGCCATTCGCTGGATGAGATCCTCCATCTGGCAGACAGAGTGATGGCACTGGAAAACGGTCAGGTGAAAGCCTTTGGCGCGCTGGAGGAAGTGTGGGGCAGTAGCGTGATGAATCCGTGGCTGCCGAAagag                                                         <  2:247784/151‑1 (MQ=255)

                                                                             cGATGTTGTATGTCAGCCATTCGCTGGATGAGATCCTCCATCTGGCAGACAGAGTGATGGCACTGGAAAACGGTCAGGTGAAAGCCTTTGGCGCGCTGGAGGAAGTGTGGGGCAGTAGCGTGATGAATCCGTGGCTGCCGAAAGAGcaaca                                                    <  1:133606/151‑1 (MQ=255)

                                                                                      aTGTCAGCCATTCGCTGGATGAGATCCTCCATCTGGCAGACAGAGTGATGGTACTGGAAAACGGTCAGGTGAAAGCCTTTGGCGCGCTGGAGGAAGTGTGGGGCAGTAGCGTGATGAATCCGTGGCTGCCGAAAGAGCAACAAAGTAGCAt                                           >  2:544887/1‑151 (MQ=255)

                                                                                          cAGCCATTCGCTGGATGAGATCCTCCATCTGGCAGACAGAGTGATGGCACTGGAAAACGGTCAGGTGAAAGCCTTTGGCGCGCTGGAGGAAGTGTGGGGCAGTAGCGTGATGAATCCGTGGCTGCCGAAAGAGCAACAAAGTAGCATTCt                                        <  1:548962/150‑1 (MQ=255)

                                                                                                  cGCTGGATGAGATCCTCCATCTGGCAGACAGAGTGATGGCACTGGAAAACGGTCAGGTGAAAGCCTTTGGCGCGCTGGAGGAAGTGTGGGGCAGTAGCGTGATGAATCCGTGGCTGCCGAAAGAGCAACAAAGTAGCATTCTGAAAGTGAc                               >  2:27061/1‑151 (MQ=255)

                                                                                                   gCTGGATGAGATCCTCCATCTGGCAGACAGAGTGATGGCACTGGAAAACGGTCAGGTGAAAGCCTTTGGCGCGCTGGAGGAAGTGTGGGGCAGTAGCGTGATGAATCCGTGGCTGCCGAAAGAGCAACAAAGTAGCATTCTGAAAGTGACg                              <  2:473494/151‑1 (MQ=255)

                                                                                                        aTGAGATCCTCCATCTGGCAGACAGAGTGATGGCACTGGAAAACGGTCAGGTGAAAGCCTTTGGCGCGCTGGAGGAAGTGTGGGGCAGTAGCGTGATGAATCCGTGGCTGCCGAAAGAGCAACAAAGTAGCATTCTGAAAGTGACGGTGCt                         <  1:452293/151‑1 (MQ=255)

                                                                                                         tGAGATCCTCCATCTGGCAGACAGAGTGATGGCACTGGAAAACGGTCAGGTGAAAGCCTTTGGCGCGCTGGAGGAAGTGTGGGGCAGTAGCGTGATGAATCCGTGGCTGCCGAAAGAGCAACAAAGTAGCATTCTGAAAGTGACGGTGCtt                        >  2:26473/1‑151 (MQ=255)

                                                                                                            gaTCCTCCATCTGGCAGACAGAGTGATGGCACTGGAAAACGGTCAGGTGAAAGCCTTTGGCGCGCTGGAGGAAGTGTGGGGCAGTAGCGTGATGAATCCGTGGCTGCCGAAAGAGCAACAAAGTAGCATTCTGAAAGTGACGGTGCTTGAg                     <  2:19034/151‑1 (MQ=255)

                                                                                                               cctccATCTGGCAGACAGAGTGATGGCACTGGAAAACGGTCAGGTGAAAGCCTTTGGCGCGCTGGAGGAAGTGTGGGGCAGTAGCGTGATGAATCCGTGGCTGCCGAAAGAGCAACAAAGTAGCATTCTGAAAGTGACGGTGCTTGAGcat                  >  1:321677/1‑151 (MQ=255)

                                                                                                                 tccATCTGGCAGACAGAGTGATGGCACTGGAAAACGGTCAGGTGAAAGCCTTTGGCGCGCTGGAGGAAGTGTGGGGCAGTAGCGTGATGAATCCGTGGCTGCCGAAAGAGCAACAAAGTAGCATTCTGAAAGTGACGGTGCTTGAGcatc                 >  2:546387/1‑150 (MQ=255)

                                                                                                                             acagaGTGATGGCACTGGAAAACGGTCAGGTGAAAGCCTTTGGCGCGCTGGAGGAAGTGTGGGGCAGTAGCGTGATGAATCCGTGGCTGCCGAAAGAGCAACAAAGTAGCATTCTGAAAGTGACGGTGCTTGAGCATCATCCGCATTAcgc    <  2:68532/151‑1 (MQ=255)

                                                                                                                               agagTGATGGCACTGGAAAACGGTCAGGTGAAAGCCTTTGGCGCGCTGGAGGAAGTGTGGGGCAGTAGCGTGATGAATCCGTGGCTGCCGAAAGAGCAACAAAGTAGCATTCTGAAAGTGACGGTGCTTGAGCATCATCCGCATTACGCGa  <  1:164556/151‑1 (MQ=255)

GCTGGCGTCACTGGATATTCCGCGTAAACGCGAACTGTTGCCTTATCTGCAACGGCTGACACGGGAAATCAACATTCCGATGTTGTATGTCAGCCATTCGCTGGATGAGATCCTCCATCTGGCAGACAGAGTGATGGTACTGGAAAACGGTCAGGTGAAAGCCTTTGGCGCGCTGGAGGAAGTGTGGGGCAGTAGCGTGATGAATCCGTGGCTGCCGAAAGAGCAACAAAGTAGCATTCTGAAAGTGACGGTGCTTGAGCATCATCCGCATTACGCGA  >  NC_000913/797018‑797295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A