Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	807,115	T→C	Q56R (CAA→CGA)	ybhC ←	acyl‑CoA thioesterase, lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	807,115	0	T	C	100.0%	101.4 / NA	28	Q56R (CAA→CGA)	ybhC	acyl‑CoA thioesterase, lipoprotein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (12/16); total (12/16)

CGCATCTACCGCCGCCTGAATCGTGGTATGCGTTACACCTTGAGTGCCCGCCGGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGTGTCAGGGATGCAAAATAGTGTTGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAGGTACCAGGCGCGGTTTGATCAGAAGGACGTTGATCGGGCGGGGTTGAGCTACAGGCGGTCAGCGTCACGCCAAAAGCCAATGCCAGCGCCA  >  NC_000913/806973‑807256

cGCATCTACCGCCGCCTGAATCGTGGTATGCGTTACACCTTGAGTGCCCGCCGGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGTGTCAGGGATGCAAAATAGTGTCGAGCATCg                                                                                                                                       <  1:543220/151‑1 (MQ=255)

  cATCTACCGCCGCCTGAATCGTGGTATGCGTTACACCTTGAGTGCCCGCCGGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGTGTCAGGGATGCAAAATAGTGTCGAGCATCGaa                                                                                                                                     <  1:608289/151‑1 (MQ=255)

         cgccgcCTGAATCGTGGTATGCGTTACACCTTGAGTGCCCGCCGGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGTGTCAGGGATGCAAAATAGTGTCGAGCATCGAAATTCTgc                                                                                                                              <  2:442978/151‑1 (MQ=255)

            cgccTGAATGGTGGTATGCGTTACTCCTTGAGTGGCCGCCGTGCCGACAAGAAAGTCAGGTTGCTCAGGCAGGTTAATCGGGTAAGGATTCCACGCTGCAGCACCTGGTGTCAGGGATGCAAAATAGTGTCGAGCATCGAAATTCTgcgc                                                                                                                            <  2:439084/150‑1 (MQ=255)

                             gCGTTACACCTTGAGTGCCCGCCGGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGTGTCAGGGATGCAAAATAGTGTCGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGaa                                                                                                           <  1:465782/150‑1 (MQ=255)

                              cGTTACACCTTGAGTGCCCGCCGGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGTGTCAGGGATGCAAAATAGTGTCGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATc                                                                                                         <  2:58246/151‑1 (MQ=255)

                                        tGAGTGCCCGCCGGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGTGTCAGGGATGCAAAATAGTGTCGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCgaga                                                                                                 <  1:611413/149‑1 (MQ=255)

                                         gAGTGCCCGCCGGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGTGTCAGGGATGCAAAATAGTGTCGAGCATCGAAATTCTgcgc                                                                                                                            <  1:423287/121‑1 (MQ=255)

                                         gAGTGCCCGCCGGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGTGTCAGGGATGCAAAATAGTGTCGAGCATCGAAATTCTgcgc                                                                                                                            >  2:423280/1‑121 (MQ=255)

                                              cccGCCGGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGTGTCAGGGATGCAAAATAGTGTCGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAGGTAc                                                                                         >  1:387860/1‑151 (MQ=255)

                                                    gggCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGTGTCAGGGATGCAAAATAGTGTCGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAGGTACCAGgcg                                                                                   <  1:608128/151‑1 (MQ=255)

                                                        cGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGTGTCAGGGATGCAAAATAGTGTCGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAGGTACCAGGCGCgg                                                                                <  1:125500/150‑1 (MQ=255)

                                                        cGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGTGTCAGGGATGCAAAATAGTGTCGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAGGTACCAGGCGCgg                                                                                >  2:125497/1‑150 (MQ=255)

                                                            aacaaAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGTGTCAGGGATGCAAAATAGTGTCGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAGGTACCAGGCGCGGTTTg                                                                            >  2:233398/1‑150 (MQ=255)

                                                                 aGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGTGTCAGGGATGCAAAATAGTGTCGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAGGTACCAGGCGCGGTTTGATc                                                                         >  2:317251/1‑148 (MQ=255)

                                                                                         tCGGGGAAGGATTCCACGCTGCAGCACCTGGTGTCAGGGATGCAAAATAGTGTCGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAGGTACCAGGCGCGGTTTGATCAGAAGGACGTTGATCGGGCGGGGTTg                                               >  2:471268/1‑150 (MQ=255)

                                                                                            gggAAGGATTCCACGCTGCAGCACCTGGTGTCAGGGATGCAAAATAGTGTCGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAGGTACCAGGCGCGGTTTGATCAGAAGGACGTTGATCGGGCGGGGTTGAGCt                                           <  1:58673/151‑1 (MQ=255)

                                                                                             ggAAGGATTCCACGCTGCAGCACCTGGTGTCAGGGATGCAAAATAGTGTCGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAGGTACCAGGCGCGGTTTGATCAGAAGGACGTTGATCGGGCGGGGTTGAGCTa                                          <  2:54465/151‑1 (MQ=255)

                                                                                             ggAAGGATTCCACGCTGCAGCACCTGGTGTCAGGGATGCAAAATAGTGTCGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAGGTACCAGGCGCGGTTTGATCAGAAGGACGTTGATCGGGCGGGGTTGAGCTa                                          <  2:76024/151‑1 (MQ=255)

                                                                                                  gATTCCACGCTGCAGCACCTGGTGTCAGGGATGCAAAATAGTGTCGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAGGTACCAGGCGCGGTTTGATCAGAAGGACGTTGATCGGGCGGGGTTGAGCTACAgg                                      >  2:452087/1‑150 (MQ=255)

                                                                                                      ccACGCTGCAGCACCTGGTGTCAGGGATGCAAAATAGTGTCGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAGGTACCAGGCGCGGTTTGATCAGAAGGACGTTGATCGGGCGGGGTTGAGCTACAGGCGGTc                                 <  2:63235/151‑1 (MQ=255)

                                                                                                       cACGCTGCAGCACCTGGTGTCAGGGATGCAAAATAGTGTCGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAGGTACCAGGCGCGGTTTGATCAGAAGGACGTTGATCGGGCGGGGTTGAGCTACAGGCGGTCa                                <  2:298634/151‑1 (MQ=255)

                                                                                                              cagcaCCTGGTGTCAGGGATGCAAAATAGTGTCGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAGGTACCAGGCGCGGTTTGATCAGAAGGACGTTGATCGGGCGGGGTTGAGCTACAGGCGGTCAGCGTc                           >  2:189871/1‑149 (MQ=255)

                                                                                                                gcaCCTGGTGTCAGGGATGCAAAATAGTGTCGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAGGTACCAGGCGCGGTTTGATCAGAAGGACGTTGATCGGGCGGGGTTGAGCTACAGGCGGTCAGCGTCACGc                       >  1:594565/1‑151 (MQ=255)

                                                                                                                                    aaaaTAGTGTCGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAGGTACCAGGCGCGGTTTGATCAGAAGGACGTTGATCGGGCGGGGTTGAGCTACAGGCGGTCAGCGTCACGCCAAAAGCCAATGCCAGCGcc   >  1:445036/1‑151 (MQ=255)

                                                                                                                                     aaaTAGTGTCGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAGGTACCAGGCGCGGTTTGATCAGAAGGACGTTGATCGGGCGGGGTTGAGCTACAGGCGGTCAGCGTCACGCCAAAAGCCAATGCCAGCGCCa  <  1:420590/151‑1 (MQ=255)

                                                                                                                                       aTAGTGTCGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAGGTACCAGGCGCGGTTTGATCAGAAGGACGTTGATCGGGCGGGGTTGAGCTACAGGCGGTCAGCGTCACGCCCAAAGCCAATGCCAGCGcc   >  1:15409/1‑148 (MQ=255)

                                                                                                                                       aTAGTGTCGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAGGTACCAGGCGCGGTTTGATCAGAAGGACGTTGATCGGGCGGGGTTGAGCTACAGGCGGTCAGCGTCACGCCAAAAGCCAATGCCAGCGcc   >  1:520087/1‑148 (MQ=255)

CGCATCTACCGCCGCCTGAATCGTGGTATGCGTTACACCTTGAGTGCCCGCCGGGCCGACAACAAAGTCAGGTTGCGCAGGCAGGGTAATCGGGGAAGGATTCCACGCTGCAGCACCTGGTGTCAGGGATGCAAAATAGTGTTGAGCATCGAAATTCTGCGCTTCTTTTGCCGACAGAATCGGGCGAGAAGAGGTACCAGGCGCGGTTTGATCAGAAGGACGTTGATCGGGCGGGGTTGAGCTACAGGCGGTCAGCGTCACGCCAAAAGCCAATGCCAGCGCCA  >  NC_000913/806973‑807256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A