Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	964,634	T→C	R105R (CGT→CGC)	ycaI →	ComEC family inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	964,634	0	T	C	100.0%	150.7 / NA	40	R105R (CGT→CGC)	ycaI	ComEC family inner membrane protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (18/22); total (18/22)

GTTTGGGGCATATTATCAGCAAAGCAAATTTTGTGGGCAGGAGAAACCTTAACTGGCGCGACGCAGGATGCAATTGTTGAGATCACTGCAACTGACGGCATGACCACTCATTACGGTCAAATTACTCATCTACAAGGTCGACGTATATTCCCTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGCAAGCGGTTTGTGCTGGACAACAATGGTCAATGAAACTCAAAGTTCGTGCAGTTCATGGCCAACTTAATGATGGCGGCTTTGATAGCCAGCGTTAT  >  NC_000913/964491‑964775

gTTTGGGGCATATTATCAGCAAAGCAAATTTTGTGGGCAGGAGAAACCTTAACTGGCGCGACGCAGGATGCAATTGTTGAGATCACTGCAACTGACGGCATGACCACTCATTACGGTCAAATTACTCATCTACAAGGTCGACGCATATTcc                                                                                                                                        >  1:501155/1‑151 (MQ=255)

  ttGGGGCATATTATCAGCAAAGCAAATTTTGTGGGCAGGAGAAACCTTAACTGGCGCGACGCAGGATGCAATTGTTGAGATCACTGCAACTGACGGCATGACCACTCATTACGGTCAAATTACTCATCTACAAGGTCGACGCATATTCCCt                                                                                                                                      >  2:125901/1‑151 (MQ=255)

   tGGGGCATATTATCAGCAAAGCAAATTTTGTGGGCAGGAGAAACCTTAACTGGCGCGACGCAGGATGCAATTGTTGAGATCACTGCAACTGACGGCATGACCACTCATTACGGTCAAATTACTCATCTACAAGGTCGACGCATATTCCCTg                                                                                                                                     >  1:51058/1‑151 (MQ=255)

    ggggCATATTATCAGCAAAGCAAATTTTGTGGGCAGGAGAAACCTTAACTGGCGCGACGCAGGATGCAATTGTTGAGATCACTGCAACTGACGGCATGACCACTCATTACGGTCAAATTACTCATCTACAAGGTCGACGCATATTCCCTg                                                                                                                                     <  2:160635/150‑1 (MQ=255)

     gggCATATTATCAGCAAAGCAAATTTTGTGGGCAGGAGAAACCTTAACTGGCGCGACGCAGGATGCAATTGTTGAGATCACTGCAACTGACGGCATGACCACTCATTACGGTCAAATTACTCATCTACAAGGTCGACGCATATTCCCTGCg                                                                                                                                   <  2:197380/151‑1 (MQ=255)

                  gcaaagcaaaTTTTGTGGGCAGGAGAAACCTTAACTGGCGCGACGCAGGATGCAATTGTTGAGATCACTGCAACTGACGGCATGACCACTCATTACGGTCAAATTACTCATCTACAAGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGa                                                                                                                      <  1:110751/151‑1 (MQ=255)

                        caaaTTTTGTGGGCAGGAGAAACCTTAACTGGCGCGACGCAGGATGCAATTGTTGAGATCACTGCAACTGACGGCATGACCACTCATTACGGTCAAATTACTCATCTACAAGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATg                                                                                                                >  1:19998/1‑151 (MQ=255)

                         aaaTTTTGTGGGCAGGAGAAACCTTAACTGGCGCGACGCAGGATGCAATTGTTGAGATCACTGCAACTGACGGCATGACCACTCATTACGGTCAAATTACTCATCTACAAGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATgg                                                                                                               <  2:187625/151‑1 (MQ=255)

                          aaTTTTGTGGGCAGGAGAAACCTTAACTGGCGCGACGCAGGATGCAATTGTTGAGATCACTGCAACTGACGGCATGACCACTCATTACGGTCAAATTACTCATCTACAAGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATGGc                                                                                                              >  2:191798/1‑151 (MQ=255)

                             tttGTGGGCAGGAGAAACCTTAACTGGCGCGACGCAGGATGCAATTGTTGAGATCACTGCAACTGACGGCATGACCACTCATTACGGTCAAATTACTCATCTACAAGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATGGCGa                                                                                                            >  1:632830/1‑150 (MQ=255)

                                       ggAGAAACCTTAACTGGCGCGACGCAGGATGCAATTGTTGAGATCACTGCAACTGACGGCATGACCACTCATTACGGTCAAATTACTCATCTACAAGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGc                                                                                                 <  2:170383/151‑1 (MQ=255)

                                        gagaAACCTTAACTGGCGCGACGCAGGATGCAATTGTTGAGATCACTGCAACTGACGGCATGACCACTCATTACGGTCAAATTACTCATCTACAAGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGCa                                                                                                <  1:281175/151‑1 (MQ=255)

                                           aaaCCTTAACTGGCGCGACGCAGGATGCAATTGTTGAGATCACTGCAACTGACGGCATGACCACTCATTACGGTCAAATTACTCATCTACAAGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGCAAGc                                                                                             <  2:341108/151‑1 (MQ=255)

                                            aaCCTTAACTGGCGCGACGCAGGATGCAATTGTTGAGATCACTGCAACTGACGGCATGACCACTCATTACGGTCAAATTACTCATCTATAAGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGCAAGCg                                                                                            >  1:277216/1‑151 (MQ=255)

                                                  aaCTGGCGCGACGCAGGATGCAATTGTTGAGATCACTGCAACTGACGGCATGACCACTCATTACGGTCAAATTACTCATCTACAAGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGCAAGCGGTTtgt                                                                                      >  2:537029/1‑151 (MQ=255)

                                                          cgACGCAGGATGCAATTGTTGAGATCACTGCAACTGACGGCATGACCACTCATTACGGTCAAATTACTCATCTACAAGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGCAAGCGGTTTGTGCTGGaca                                                                              <  2:400308/151‑1 (MQ=255)

                                                            aCGCAGGATGCAATTGTTGAGATCACTGCAACTGACGGCATGACCACTCATTACGGTCAAATTACTCATCTACAAGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGCAAGCGGTTTGTGCTGGacaac                                                                            >  1:279064/1‑151 (MQ=255)

                                                              gCAGGATGCAATTGTTGAGATCACTGCAACTGACGGCATGACCACTCATTACGGTCAAATTACTCATCTACAAGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGCAAGCGGTTTGTGCTGGacaaca                                                                           >  2:86845/1‑150 (MQ=255)

                                                               cAGGATGCAATTGTTGAGATCACTGCAACTGACGGCATGACCACTCATTACGGTCAAATTACTCATCTACAAGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGCAAGCGGTTTGTGCTGGACAACAAt                                                                         >  2:418741/1‑151 (MQ=255)

                                                                 ggATGCAATTGTTGAGATCACTGCAACTGACGGCATGACCACTCATTACGGTCAAATTACTCATCTACAAGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGCAAg                                                                                              <  1:614593/128‑1 (MQ=255)

                                                                 ggATGCAATTGTTGAGATCACTGCAACTGACGGCATGACCACTCATTACGGTCAAATTACTCATCTACAAGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGCAAg                                                                                              >  2:614576/1‑128 (MQ=255)

                                                                     gCAATTGTTGAGATCACTGCAACTGACGGCATGACCACTCATTACGGTCAAATTACTCATCTACAAGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGCAAGCGGTTTGTGCTGGACAACAATGGTCa                                                                    <  1:22744/150‑1 (MQ=255)

                                                                                   cACTGCAACTGACGGCATGACCACTCATTACGGTCAAATTACTCATCTACAAGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGCAAGCGGTTTGTGCTGGACAACAATGGTCAATGAAACTCAAAGtt                                                     <  1:412420/151‑1 (MQ=255)

                                                                                    aCTGCAACTGACGGCATGACCACTCATTACGGTCAAATTACTCATCTACAAGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGCAAGCGGTTTGTGCTGGACAACAATGGTCAATGAAACTCAAAGTTc                                                    <  1:305903/151‑1 (MQ=255)

                                                                                       gCAACTGACGGCATGACCACTCATTACGGTCAAATTACTCATCTACAAGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGCAAGCGGTTTGTGCTGGACAACAATGGTCAATGAAACTCAAAGTTCg                                                   <  1:499674/149‑1 (MQ=255)

                                                                                         aaCTGACGGCATGACCACTCATTACGGTCAAATTACTCATCTACAAGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGCAAGCGGTTTGTGCTGGACAACAATGGTCAATGAAACTCAAAGTTCGTGc                                                >  2:67726/1‑150 (MQ=255)

                                                                                               cGGCATGACCACTCATTACGGTCAAATTACTCATCTACAAGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGCAAGCGGTTTGTGCTGGACAACAATGGTCAATGAAACTCAAAGTTCGTGCAGTTCAt                                         >  1:576671/1‑151 (MQ=255)

                                                                                               cGGCATGACCACTCATTACGGTCAAATTACTCATCTACAAGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGCAAGCGGTTTGTGCTGGACAACAATGGTCAATGAAACTCAAAGTTCGTGCAGTTCAt                                         >  1:322853/1‑151 (MQ=255)

                                                                                                     gACCACTCATTACGGTCAAATTACTCATCTACAAGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGCAAGCGGTTTGTGCTGGACAACAATGGTCAATGAAACTCAAAGTTCGTGCAGTTCATGGCCa                                    <  1:67726/150‑1 (MQ=255)

                                                                                                            cATTACGGTCAAATTACTCATCTACAAGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGCAAGCGGTTTGTGCTGGACAACAATGGTCAATGAAACTCAAAGTTCGTGCAGTTCATGGCCAACTTAatg                            <  2:197416/151‑1 (MQ=255)

                                                                                                            cATTACGGTCAAATTACTCATCTACAAGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGCAAGCGGTTTGTGCTGGACAACAATGGTCAATGAAACTCAAAGTTCGTGCAGTTCATGGCCAACTTAat                             <  2:54728/150‑1 (MQ=255)

                                                                                                                  ggTCAAATTACTCATCTACAAGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGCAAGCGGTTTGTGCTGGACAACAATGGTCAATGAAACTCAAAGTTCGTGCAGTTCATGGCCAACTTAATGATggcg                      <  1:191803/151‑1 (MQ=255)

                                                                                                                    tCAAATTACGCATCTACAAGGTCGACGCATATTCCGTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGCAAGCGGTTTGTGCTGGACAACAATGGTCAATGAAACTCAAAGTTCGTGCAGTTCATGGCCAACTTAATGATggcg                      <  2:95888/149‑1 (MQ=255)

                                                                                                                      aaaTTACTCATCTACAAGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGCAAGCGGTTTGTGCTGGACAACAATGGTCAATGAAACTCAAAGTTCGTGCAGTTCATGGCCAACTTAATGATGGCGGCtt                  <  2:109413/151‑1 (MQ=255)

                                                                                                                           aCTCATCTACAAGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGCAAGCGGTTTGTGCTGGACAACAATGGTCAATGAAACTCAAAGTTCGTGCAGTTCATGGCCAACTTAATGATGGCGGCTTTGATa             <  1:215855/151‑1 (MQ=255)

                                                                                                                                 cTACAAGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGCAAGCGGTTTGTGCTGGACAACAATGGTCAATGAAACTCAAAGTTCGTGCAGTTCATGGCCAACTTAATGATGGCGGCTTTGATAGCCAGc       >  1:535468/1‑151 (MQ=255)

                                                                                                                                   aCAAGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGCAAGCGGTTTGTGCTGGACAACAATGGTCAATGAAACTCAAAGTTCGTGCAGTTCATGGCCAACTTAATGATGGCGGCTTTGATAGCCAGCg      <  1:175747/150‑1 (MQ=255)

                                                                                                                                   aCAAGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGCAAGCGGTTTGTGCTGGACAACAATGGTCAATGAAACTCAAAGTTCGTGCAGTTCATGGCCAACTTAATGATGGCGGCTTTGATAGCCAGCGt     >  1:266692/1‑151 (MQ=255)

                                                                                                                                      aGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGCAAGCGGTTTGTGCTGGACAACAATGGTCAATGAAACTCAAAGTTCGTGCAGTTCATGGCCAACTTAATGATGGCGGCTTTGATAGCCAGCGTTAt  >  2:359664/1‑151 (MQ=255)

                                                                                                                                      aGGTCGACGCATATTCCCTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGCAAGCGGTTTGTGCTAGACAACAATGGTCAATGAAACTCAAAGTTCGTGCAGTTCATGGCCAACTTAATGATGGCGGCTTTGATAGCCAGCGTTAt  <  1:480805/151‑1 (MQ=255)

GTTTGGGGCATATTATCAGCAAAGCAAATTTTGTGGGCAGGAGAAACCTTAACTGGCGCGACGCAGGATGCAATTGTTGAGATCACTGCAACTGACGGCATGACCACTCATTACGGTCAAATTACTCATCTACAAGGTCGACGTATATTCCCTGCGTCAGGTCTCGTGATGTATGGCGAATATCTTCCGCAAGCGGTTTGTGCTGGACAACAATGGTCAATGAAACTCAAAGTTCGTGCAGTTCATGGCCAACTTAATGATGGCGGCTTTGATAGCCAGCGTTAT  >  NC_000913/964491‑964775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A