Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,029,884	C→T	R29H (CGC→CAC)	rlmI ←	23S rRNA m(5)C1962 methyltransferase, SAM‑dependent

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,029,884	0	C	T	100.0%	107.1 / NA	28	R29H (CGC→CAC)	rlmI	23S rRNA m(5)C1962 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (14/14); total (14/14)

AAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTGTCGATGCAAAGAAATGGGCGTCATTGTCCGGAGTTTTCCATGGATTTG  >  NC_000913/1029737‑1030031

aaaGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGTggg                                                                                                                                                  >  2:291885/1‑151 (MQ=255)

   gCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGTGGGCaa                                                                                                                                               <  2:610817/151‑1 (MQ=255)

        gTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGTGGGCAACGGcc                                                                                                                                          <  1:410403/151‑1 (MQ=255)

          cGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGTGGGCAACGGcccc                                                                                                                                        <  1:339247/151‑1 (MQ=255)

                aCTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGTGGGCAACGGCCCCGGaaa                                                                                                                                   >  2:56393/1‑150 (MQ=255)

                    aGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGTGGGCAACGGCCCCGGAAAAGAcc                                                                                                                              >  2:448633/1‑151 (MQ=255)

                                       aCGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGTGGGCAACGGCCCCGGAAAAGACCCACGGATGGCGACGAAGt                                                                                                            >  1:590652/1‑150 (MQ=255)

                                          cgcgcCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGTGGGCAACGGCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATg                                                                                                        <  2:21566/151‑1 (MQ=255)

                                            cgcCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGTGGGCAACGGCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGAt                                                                                                      >  1:383347/1‑151 (MQ=255)

                                              cccGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGCTAACCCTTTTCCCTGATGCTCAACAATATCGATGGTTTCACCGCGGCTGGCTTTACCTTCCATGTGGGCAACGGCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGAt                                                                                                      <  2:105591/149‑1 (MQ=255)

                                                                 cGAATAAGCGCCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGTGGGCAACGGCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAac                                                                                 >  1:440370/1‑151 (MQ=255)

                                                                 cGAATAAGCGCCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGTGGGCAACGGCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAac                                                                                 >  1:534015/1‑151 (MQ=255)

                                                                                 gCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGTGGGCAACGGCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCa                                                                 <  2:442344/151‑1 (MQ=255)

                                                                                          tttCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGTGGGCAACGGCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTAc                                                         <  1:392403/150‑1 (MQ=255)

                                                                                                tgatgaTCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGTGGGCAACGGCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTg                                                  >  2:584145/1‑151 (MQ=255)

                                                                                                 gatgatCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGTGGGCAACGGCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCt                                                   <  2:25837/149‑1 (MQ=255)

                                                                                                 gatgatCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGTGGGCAACGGCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTg                                                  <  2:84014/150‑1 (MQ=255)

                                                                                                       caacaaTATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGTGGGCAACGGCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTGTCGATGc                                           >  2:442512/1‑151 (MQ=255)

                                                                                                          caaTATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGTGGGCAACGGCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTGTCGATGCaaa                                        <  1:148606/151‑1 (MQ=255)

                                                                                                          caaTATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGTGGGCAACGGCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTGTCGATGCaa                                         >  2:446116/1‑150 (MQ=255)

                                                                                                                   tGGTTTCACCGAGGCTGGCTTTACCTTCCATGTGGGCAACGGCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTGTCGATGCAAAGAAATGGGc                               >  2:417842/1‑151 (MQ=255)

                                                                                                                   tGGTTTCACCGAGGCTGGCTTTACCTTCCATGTGGGCAACGGCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTGTCGATGCAAAGAAATGGGc                               >  1:497837/1‑151 (MQ=255)

                                                                                                                    ggTTTCACCGAGGCTGGCTTTACCTTCCATGTGGGCAACGGCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTGTCGATGCAAAGAAATGGGCg                              <  2:47956/151‑1 (MQ=255)

                                                                                                                         cACCGAGGCTGGCTTTACCTTCCATGTGGGCAACGGCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTGTCGATGCAAAGAAATGGGCGTCAtt                         >  2:486789/1‑151 (MQ=255)

                                                                                                                          aCCGAGGCTGGCTTTACCTTCCATGTGGGCAACGGCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTGTCGATGCAAAGAAATGGGCGTCATTg                        >  1:211402/1‑151 (MQ=255)

                                                                                                                                        tACCTTCCATGTGGGCAACGGCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTGTCGATGCAAAGAAATGGGCGTCATTGTCCGGAGTTTTcc           <  1:388820/150‑1 (MQ=255)

                                                                                                                                              ccATGTGGGCAACGGCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTGTCGATGCAAAGAAATGGGCGTCATTGTCCGGAGTTTTCCATGGAtt    <  2:497827/151‑1 (MQ=255)

                                                                                                                                                aTGTGGGCAACGGCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTGTCGATGCAAAGAAATGGGCGTCATTGTCCGGAGTTTTCCATGGATTTg  <  2:38857/151‑1 (MQ=255)

AAAGCAATGTCGATAGACTCAGACGGGTCAAACGTCCAGACGCGCGCCCGGATTTGCGAAGCTGGCGAATAAGCGCCGCGTGCTAACCATTTTCCCTGATGATCAACAATATCGATGGTTTCACCGAGGCTGGCTTTACCTTCCATGCGGGCAACGGCCCCGGAAAAGACCCACGGATGGCGACGAAGTAATGATTTTTCGCGCCCTTTGGCTAACACTAAACGTACACTCATAATTTACTTTTCTGTCGATGCAAAGAAATGGGCGTCATTGTCCGGAGTTTTCCATGGATTTG  >  NC_000913/1029737‑1030031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A