Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,423,878	T→C	F33L (TTT→CTT)	trkG →	Rac prophage; potassium transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,423,878	0	T	C	100.0%	149.1 / NA	39	F33L (TTT→CTT)	trkG	Rac prophage; potassium transporter subunit
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (19/20); total (19/20)

GTATAATCATAATATTGCAGCAAGGTGGTTATAATTGAAAGAATATTTAGATATGAATACATCTCATGTAAGAGTTGTTACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGTTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTGGCATTCAATTACGTACCCGTGATGGGTTTATTATAATTGTAATGTTTTGG  >  NC_000913/1423730‑1424021

gTATAATCATAATATTGCAGCAAGGTGGTTATAATTGAAAGAATATTTAGATATGAATACATCTCATGTAAGAGTTGTTACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGctt                                                                                                                                               <  1:479081/151‑1 (MQ=255)

       cATAATATTGCAGCAAGGTGGTTATAATTGAAAGAATATTTAGATATGAATACATCTCATGTAAGAGTTGTTACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGCTTTATaaa                                                                                                                                         <  1:174246/150‑1 (MQ=255)

                         tggtTATAATTGAAAGAATATTTAGATATGAATACATCTCATGTAAGAGTTGTTACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTgtttgttt                                                                                                                      >  1:277573/1‑151 (MQ=255)

                              ataATTGAAAGAATATTTAGATATGAATACATCTCATGTAAGAGTTGTTACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCtt                                                                                                                  <  1:260802/150‑1 (MQ=255)

                                        gAATATTTAGATATGAATACATCTCATGTAAGAGTTGTTACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCg                                                                                                       >  1:280275/1‑151 (MQ=255)

                                            atTTAGATATGAATACATCTCATGTAAGAGTTGTTACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTtat                                                                                                   >  1:169261/1‑151 (MQ=255)

                                            atTTAGATATGAATACATCTCATGTAAGAGTTGTTACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTtat                                                                                                   <  1:479259/151‑1 (MQ=255)

                                             tttAGATATGAATACATCTCATGTAAGAGTTGTTACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTtat                                                                                                   >  1:556190/1‑150 (MQ=255)

                                               tAGATATGAATACATCTCATGTAAGAGTTGTTACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTatat                                                                                                 <  1:289987/150‑1 (MQ=255)

                                                aGATATGAATACATCTCATGTAAGAGTTGTTACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATAtt                                                                                                <  1:44403/150‑1 (MQ=255)

                                                  atatGAATACATCTCATGTAAGAGTTGTTACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATAttttt                                                                                             >  2:137610/1‑151 (MQ=255)

                                                               tcATGTAAGAGTTGTTACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATtggtgg                                                                                 >  1:38297/1‑150 (MQ=255)

                                                                cATGTAAGAGTTGTTACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCg                                                                               <  2:168178/151‑1 (MQ=255)

                                                                cATGTAAGAGTTGTTACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCg                                                                               >  2:462445/1‑151 (MQ=255)

                                                                            gttACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGtat                                                                    >  2:92680/1‑150 (MQ=255)

                                                                             ttACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCAt                                                                                       <  2:299894/130‑1 (MQ=255)

                                                                             ttACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCAt                                                                                       >  1:299901/1‑130 (MQ=255)

                                                                             ttACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATAc                                                                  >  1:173713/1‑151 (MQ=255)

                                                                                 tCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACt                                                              >  1:585761/1‑151 (MQ=255)

                                                                                  cATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACt                                                              >  1:98604/1‑150 (MQ=255)

                                                                                   atatGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTaa                                                            <  1:554234/151‑1 (MQ=255)

                                                                                     atGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAg                                                           <  2:528790/150‑1 (MQ=255)

                                                                                          tgGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCt                                                     >  2:523018/1‑151 (MQ=255)

                                                                                                 cTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTGGCATTc                                              >  2:124566/1‑151 (MQ=255)

                                                                                                 cTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTGGCATTc                                              >  2:170776/1‑151 (MQ=255)

                                                                                                  tGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTGGCATTc                                              >  1:410534/1‑150 (MQ=255)

                                                                                                       tGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTGGCATTCAATTa                                         <  1:250914/150‑1 (MQ=255)

                                                                                                       tGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTGGCATTCAATTa                                         <  2:357686/150‑1 (MQ=255)

                                                                                                        ggCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGCTTTATAAAGAAAAAGGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTGGCATTCAATTACg                                       >  1:571207/1‑151 (MQ=255)

                                                                                                            ctATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTGGCATTCAATTACGTAcc                                   >  1:224198/1‑151 (MQ=255)

                                                                                                                     ttCAATGTTGCCACCAATGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTGGCATTCAATTACGTACCCGTGATgg                           <  2:77801/150‑1 (MQ=255)

                                                                                                                           gTTGCCACCAATGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTGGCATTCAATTACGTACCCGTGATGGGTttat                     <  2:10529/150‑1 (MQ=255)

                                                                                                                              gCCACCAATGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTGGCATTCAATTACGTACCCGTGATGGGTTtattat                  >  1:189966/1‑150 (MQ=255)

                                                                                                                               ccaccaATGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTCTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTGGCATTCAATTACGTACCCGTGATGGGTTTATTATaa                <  2:563570/151‑1 (MQ=255)

                                                                                                                                 accaATGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTGGCATTCAATTACGTACCCGTGATGGGTTTATTATAAtt              <  1:602377/151‑1 (MQ=255)

                                                                                                                                     aTGGTTGTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTGGCATTCAATTACGTACCCGTGATGGGTTTATTATAATTGTaa          <  2:302979/151‑1 (MQ=255)

                                                                                                                                           gTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTGGCATTCAATTACGTACCCGTGATGGGTTTATTATAATTGTAATGtttt    <  2:3422/151‑1 (MQ=255)

                                                                                                                                           gTAGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTGGCATTCAATTACGTACCCGTGATGGGTTTATTATAATTGTAATGtttt    <  1:244596/151‑1 (MQ=255)

                                                                                                                                             aGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTGGCATTCAATTACGTACCCGTGATGGGTTTATTATAATTGTAATGTTTTgg  <  2:73150/151‑1 (MQ=255)

                                                                                                                                             aGCATTGCTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTGGCATTCAATTACGTACCCGTGATGGGTTTATTATAATTGTAATGTTTTg   <  2:358122/150‑1 (MQ=255)

GTATAATCATAATATTGCAGCAAGGTGGTTATAATTGAAAGAATATTTAGATATGAATACATCTCATGTAAGAGTTGTTACTCATATGTGTGGGTTCCTGGTTTGGCTCTATAGTCTTTCAATGTTGCCACCAATGGTTGTAGCATTGTTTTATAAAGAAAAAAGCCTGTTTGTTTTCTTTATAACTTTCGTTATATTTTTTTGCATTGGTGGCGGAGCGTGGTATACAACTAAGAAATCTGGCATTCAATTACGTACCCGTGATGGGTTTATTATAATTGTAATGTTTTGG  >  NC_000913/1423730‑1424021

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A