Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F50 I1 R1
|
170 |
39.5 |
1266514 |
99.2% |
1256381 |
150.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,696,861 |
T→C |
L64L (TTA→TTG) |
uidR ← |
transcriptional repressor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,696,861 | 0 | T | C | 92.6%
| 86.7
/ ‑0.8
| 27 | L64L (TTA→TTG) | uidR | transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (12/13); total (13/14) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGAT > NC_000913/1696726‑1697007
|
cATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGCAAGATAATCGCCTg > 2:368355/1‑150 (MQ=255)
aTTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGCAAGATAATCGCCTGAATc > 2:523887/1‑150 (MQ=255)
cAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGCAAGATAATCGCCTGAATCAAgg < 2:180933/150‑1 (MQ=255)
aaCCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGCAAGATAATCGCCTGAATCAAGGct > 2:511748/1‑151 (MQ=255)
aaCCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGCAAGATAATCGCCTGAATCAAGGct > 2:269877/1‑151 (MQ=255)
aaCCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGCAAGATAATCGCCTGAATCAAGGc < 1:388660/150‑1 (MQ=255)
ttGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGCAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGaa > 2:353579/1‑150 (MQ=255)
tGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGCAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGaaa < 1:133745/150‑1 (MQ=255)
aCAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGCAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATgg < 2:337870/151‑1 (MQ=255)
gACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGCAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGAttt > 2:597302/1‑150 (MQ=255)
cGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGCAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCAt < 1:137542/151‑1 (MQ=255)
gAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGCAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATc < 1:348758/151‑1 (MQ=255)
ccTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGCAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACt < 1:627505/149‑1 (MQ=255)
cTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGCAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACtgtg > 2:109424/1‑151 (MQ=255)
cAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGCAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACTGTGaa < 2:36657/150‑1 (MQ=255)
ggTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGCAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACTGTGAAATCCAt < 2:63826/151‑1 (MQ=255)
tCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGCAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACTGTGAAATCCAtttt > 1:133402/1‑151 (MQ=255)
ccGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACTGTGAAATCCATTTTCt < 1:16551/151‑1 (MQ=255)
cGGGCCAGCGCCCTCTCCTGGTCCTGCAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACTGTGAAATCCATTTTCTGaaaaaa < 2:133399/151‑1 (MQ=255)
gCCAGCGCCCTCTCCTGGTCCTGCAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAAtct < 1:561812/151‑1 (MQ=255)
ccAGCGCCCTCTCCTGGTCCTGCAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAAtctc < 1:597182/151‑1 (MQ=255)
aGCGCCCTCTCCTGGTCCTGCAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAAtctctc > 2:631434/1‑151 (MQ=255)
aGCGCCCTCTCCTGGTCCTGCAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAAtctctc > 2:387303/1‑151 (MQ=255)
cgcCCTCTCCTGGTCCTGCAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTg > 2:68235/1‑151 (MQ=255)
cgcCCTCTCCTGGTCCTGCAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTg > 1:279614/1‑151 (MQ=255)
ccTGGTCCTGCAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCAt < 1:589248/150‑1 (MQ=255)
ccTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGAt > 2:260539/1‑151 (MQ=255)
|
CATAATTTCAACCACCAGCGCCCGTTGTCCAAAGGCTTCATGGGTGAGAGAGACAATGGACTCGACCATATAGTCAACGAAATGAATCCCTTCAATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTTCATCGAGGCACTGTGAAATCCATTTTCTGAAAAAATCTCTCTGGCAGCATTGAGGAT > NC_000913/1696726‑1697007
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
