Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F50 I1 R1
|
170 |
39.5 |
1266514 |
99.2% |
1256381 |
150.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
1,878,732 |
Δ2 bp |
coding (1018‑1019/1026 nt) |
yeaP → |
diguanylate cyclase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 1,878,731 | 0 | A | . | 100.0%
| 146.3
/ NA
| 31 | coding (1017/1026 nt) | yeaP | diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base . (10/21); total (10/21) |
| * | NC_000913 | 1,878,732 | 0 | C | . | 100.0%
| 146.3
/ NA
| 31 | coding (1018/1026 nt) | yeaP | diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (10/21); total (10/21) |
ATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCGGGAGCATAAAGAT > NC_000913/1878583‑1878875
||
aTTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTatt > 1:146367/1‑149 (MQ=255)
gTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTattcct < 1:404215/150‑6 (MQ=255)
gCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCT‑‑ATTCCTGAGTCGTATTcac < 2:611672/150‑1 (MQ=255)
ggCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCT‑‑ATTCCTGAGGCGTATTCACATCCTTTTGa < 2:213998/151‑1 (MQ=255)
ggCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCT‑‑ATTCCTGAGGCGTATTCACATCCTTTTGa < 2:199581/151‑1 (MQ=255)
agtagAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCT‑‑ATTCCTGAGGCGTATTCACATCCTTTTGATTgg > 2:621412/1‑150 (MQ=255)
agtagAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCT‑‑ATTCCTGAGGCGTATTCACATCCTTTTGATTgg < 1:244681/150‑1 (MQ=255)
agtagAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCT‑‑ATTCCTGAGGCGTATTCACATCCTTTTGATTGGt > 2:177208/1‑151 (MQ=255)
gTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCT‑‑ATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACa < 2:606782/151‑1 (MQ=255)
gTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCT‑‑ATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACa < 2:309907/151‑1 (MQ=255)
cGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCT‑‑ATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACAt < 1:152038/150‑1 (MQ=255)
tCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCT‑‑ATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCg < 2:493415/150‑1 (MQ=255)
acCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCT‑‑ATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGtatta < 1:60167/151‑1 (MQ=255)
gCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCT‑‑ATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTAttttt < 1:177213/150‑1 (MQ=255)
cAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCT‑‑ATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTcc > 2:344671/1‑151 (MQ=255)
aGACAGTTCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACTCCTTTTGTCGCGCATCCAGCGCT‑‑ATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGTTATTAttttt < 2:285432/148‑1 (MQ=255)
gATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCT‑‑ATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCgg > 1:141438/1‑151 (MQ=255)
atTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCT‑‑ATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTc < 1:288976/150‑1 (MQ=255)
aTCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCT‑‑ATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGc < 1:104230/151‑1 (MQ=255)
tCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCT‑‑ATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGc > 1:312854/1‑150 (MQ=255)
cAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCT‑‑ATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCAt < 1:344678/151‑1 (MQ=255)
gaAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCT‑‑ATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGttt > 1:143759/1‑151 (MQ=255)
acaAACAGAAAACACCTTTTGTCGCGCATCCAGCGCT‑‑ATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTgg < 1:236467/151‑1 (MQ=255)
aCAGAAAACACCTTTTGTCGCGCATCCAGCGCT‑‑ATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTggcggc < 2:297762/151‑1 (MQ=255)
cAGAAAACCCCTTTTGTCGCGCATCCAGCGCT‑‑ATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCt < 1:71777/151‑1 (MQ=255)
acCTTTTGTCGCGCATCCAGCGCT‑‑ATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGcc > 1:89918/1‑151 (MQ=255)
tttGTCGCGCATCCAGCGCT‑‑ATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCgg > 1:124395/1‑151 (MQ=255)
tGTCGCGCATCCAGCGCT‑‑ATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCggg > 1:331303/1‑150 (MQ=255)
tCGCGCATCCAGCGCT‑‑ATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCGGGAGc > 2:562789/1‑151 (MQ=255)
cATCCAGCGCT‑‑ATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCGGGAGCATaaa < 1:437680/151‑1 (MQ=255)
ccAGCGCT‑‑ATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCGGGAGCATAAAGAt < 1:268136/151‑1 (MQ=255)
ccAGCGCT‑‑ATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCGGGAGCATAAAGAt < 1:159865/151‑1 (MQ=255)
ccAGCGCT‑‑ATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCGGGAGCATAAAGAt < 2:70548/151‑1 (MQ=255)
||
ATTTGTATTATCCCGGTGCCAGTCTTGGCATAGTAGAAGTCGATCCTGAAACAACCGATGCAGACAGTGCCCTGCATGCTGCCGATATTGCGATGTATCAGGAGAAAAAACACAAACAGAAAACACCTTTTGTCGCGCATCCAGCGCTACATTCCTGAGGCGTATTCACATCCTTTTGATTGGTGATAACATGCGAATCGGTATTATTTTTCCGGTTGTAATCTTCATTACAGCGGTCGTATTTTTAGCATGGTTTTTTATTGGCGGCTATGCTGCCCCGGGAGCATAAAGAT > NC_000913/1878583‑1878875
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
