Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,040,356	T→C	A293A (GCT→GCC)	yedY →	membrane‑anchored, periplasmic TMAO, DMSO reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,040,356	0	T	C	100.0%	130.5 / NA	38	A293A (GCT→GCC)	yedY	membrane‑anchored, periplasmic TMAO, DMSO reductase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (18/20); total (18/20)

CTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGCCCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACA  >  NC_000913/2040215‑2040502

cTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCCACCGAACg                                                                                                                                            >  2:176231/1‑150 (MQ=255)

 tttAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCCACCGAACGAt                                                                                                                                          >  2:167178/1‑151 (MQ=255)

   tAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCCACCGAACGAttt                                                                                                                                        >  2:276751/1‑151 (MQ=255)

     aaGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCCACCGAACGATTTAt                                                                                                                                      >  1:374238/1‑151 (MQ=255)

                 cGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCCACCGAACGATTTATTGGTTCAggcg                                                                                                                           <  2:183875/150‑1 (MQ=255)

                 cGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCCACCGAACGATTTATTGGTTCAggcg                                                                                                                           <  1:500250/150‑1 (MQ=255)

                   aTAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCCACCGAACGATTTATTGGTTCAGGCGGCa                                                                                                                        <  1:395577/151‑1 (MQ=255)

                        cAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCCACCGAACGATTTATTGGTTCAGGCGGCATCCTc                                                                                                                   >  2:76901/1‑151 (MQ=255)

                           tATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCCACCGAACGATTTATTGGTTCAGGCGGCATCCTCGAt                                                                                                                >  2:139635/1‑151 (MQ=255)

                                  cTGACCCGCGAGCGTCCGCCGACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCCACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAgc                                                                                                         >  1:103309/1‑151 (MQ=255)

                                   tGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCCACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAgcg                                                                                                        >  2:445698/1‑151 (MQ=255)

                                    gACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCCACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAgcgc                                                                                                       >  2:76296/1‑151 (MQ=255)

                                             gCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCCACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACg                                                                                              <  1:42116/151‑1 (MQ=255)

                                               gTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCCACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGc                                                                                             <  2:508877/150‑1 (MQ=255)

                                               gTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCCACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCt                                                                                            >  2:235922/1‑151 (MQ=255)

                                                           ccTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCCACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATg                                                                                 <  2:400951/150‑1 (MQ=255)

                                                               gAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCCACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTAc                                                                            <  2:135397/151‑1 (MQ=255)

                                                               gAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCCACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTAc                                                                            <  2:504212/151‑1 (MQ=255)

                                                                 aTCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCCACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTAcgc                                                                          >  2:306309/1‑151 (MQ=255)

                                                                         gcgcCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCCACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGCCCAgg                                                                  <  2:219154/151‑1 (MQ=255)

                                                                                aCGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCCACCGAATGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGCCCAGGTGGCATc                                                           <  2:551073/151‑1 (MQ=255)

                                                                                aCGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCCACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGCCCAGGTGGCATc                                                           <  1:351579/151‑1 (MQ=255)

                                                                                aCGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCCACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGCCCAGGTGGCATc                                                           >  2:282302/1‑151 (MQ=255)

                                                                                 cGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCCACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGCCCAGGTGGCATCg                                                          >  2:73725/1‑151 (MQ=255)

                                                                                  gAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCCACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGCCCAGGTGGCATCGc                                                         >  1:385036/1‑151 (MQ=255)

                                                                                               aCGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCCACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGCCCAGGTGGCATCGCTGTATCGTGGcc                                             <  1:329328/150‑1 (MQ=255)

                                                                                                 gCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCCACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGCCCAGGTGGCATCGCTGTATCGTGGCCTgg                                          >  2:79995/1‑151 (MQ=255)

                                                                                                  ccAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCCACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGCCCAGGTGGCATCGCTGTATCGTGGCCTGGa                                         <  2:619821/151‑1 (MQ=255)

                                                                                                    aaCGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCCACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGCCCAGGTGGCATCGCTGTATCGTGGCCTGGAtt                                       <  1:590165/151‑1 (MQ=255)

                                                                                                      cGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCCACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGCCCAGGTGGCATCGCTGTATCGTGGCCTGGATTTg                                     >  2:287513/1‑151 (MQ=255)

                                                                                                             ccGTATGTTGATCACCCGCGCTGGTCACAGGCCACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGCCCAGGTGGCATCGCTGTACCGTGGCCTGGATTTGCGGgag                               <  2:346122/150‑1 (MQ=255)

                                                                                                              cGTATGTTGATCACCCGCGCTGGTCACAGGCCACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGCCCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGaa                             <  1:382023/151‑1 (MQ=255)

                                                                                                                 aTGTTGATCACCCGCGCTGGTCACAGGCCACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGCCCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAAtt                           <  1:567422/150‑1 (MQ=255)

                                                                                                                     tGATCACCCGCGCTGGTCACAGGCCACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGCCCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTa                       >  2:543406/1‑150 (MQ=255)

                                                                                                                             cgcgCTGGTCACAGGCCACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGCCCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCt              >  2:73999/1‑151 (MQ=255)

                                                                                                                                   ggTCACAGGCCACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGCCCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACa          <  1:522207/149‑1 (MQ=255)

                                                                                                                                         aGGCCACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGCCCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACa  <  2:373275/151‑1 (MQ=255)

                                                                                                                                         aGGCCACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGCCCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACa  <  2:549515/151‑1 (MQ=255)

CTTTAAAGGGATTAAATCGATAGTCAGTATTAAGCTGACCCGCGAGCGTCCGCCAACCACCTGGAATCTGGCAGCGCCTGACGAATACGGTTTTTACGCCAACGTTAATCCGTATGTTGATCACCCGCGCTGGTCACAGGCTACCGAACGATTTATTGGTTCAGGCGGCATCCTCGATGTACAGCGCCAGCCAACGCTACTGTTTAATGGTTACGCCGCCCAGGTGGCATCGCTGTATCGTGGCCTGGATTTGCGGGAGAATTTCTAAATGCGTCTGACAGCAAAACA  >  NC_000913/2040215‑2040502

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A