Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F50 I1 R1	170	39.5	1266514	99.2%	1256381	150.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,049,101	T→C	G1388G (GGT→GGC)	yeeJ →	putative adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,049,101	0	T	C	100.0%	124.8 / NA	35	G1388G (GGT→GGC)	yeeJ	putative adhesin
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (15/20); total (15/20)

TAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAA  >  NC_000913/2048969‑2049246

tAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCaggag                                                                                                                                    >  1:188826/1‑148 (MQ=255)

  gCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAAc                                                                                                                               <  2:157427/151‑1 (MQ=255)

      aTCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTc                                                                                                                           >  1:272549/1‑151 (MQ=255)

            tAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGtt                                                                                                                     >  2:397287/1‑151 (MQ=255)

                        tGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGa                                                                                                          >  1:105780/1‑150 (MQ=255)

                          aTGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAgg                                                                                                       <  1:15906/151‑1 (MQ=255)

                                  aCGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGAcc                                                                                                <  1:27725/150‑1 (MQ=255)

                                     gCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCa                                                                                            <  2:69018/151‑1 (MQ=255)

                                          tctcACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGtaat                                                                                       >  2:13698/1‑151 (MQ=255)

                                             cACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGtaa                                                                                        <  2:471489/147‑1 (MQ=255)

                                                  tGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCt                                                                                 <  2:58308/149‑1 (MQ=255)

                                                    cTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCtatat                                                                             >  1:376613/1‑151 (MQ=255)

                                                       cAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCtatatata                                                                          <  2:141399/151‑1 (MQ=255)

                                                          gCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATAtacta                                                                       <  2:252674/151‑1 (MQ=255)

                                                                   gATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCac                                                               <  1:397294/150‑1 (MQ=255)

                                                                    aTACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCacga                                                             <  1:311999/151‑1 (MQ=255)

                                                                     tACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCacgac                                                            >  1:445606/1‑151 (MQ=255)

                                                                     tACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCacgac                                                            >  2:167143/1‑151 (MQ=255)

                                                                          tctcCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCa                                                        >  1:178135/1‑150 (MQ=255)

                                                                             ccGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAAt                                                      <  2:207252/149‑1 (MQ=255)

                                                                                   gCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGc                                              <  2:253853/151‑1 (MQ=255)

                                                                                                    tataTTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACa                              >  2:102228/1‑150 (MQ=255)

                                                                                                      tatTGAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACaaa                            >  2:23072/1‑150 (MQ=255)

                                                                                                          gAGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCg                       <  2:525758/151‑1 (MQ=255)

                                                                                                           aGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCgg                      >  2:195780/1‑151 (MQ=255)

                                                                                                           aGGTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCgg                      <  1:548913/151‑1 (MQ=255)

                                                                                                             gTGAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCgggg                    <  1:449422/151‑1 (MQ=255)

                                                                                                               gAAGGATAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCGGGGt                   <  2:376606/150‑1 (MQ=255)

                                                                                                                    aTAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCGGGGTTTATCa             <  1:597084/151‑1 (MQ=255)

                                                                                                                     tAATTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCGGGGTTTATCa             >  1:301945/1‑150 (MQ=255)

                                                                                                                      aaTTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCGGGGTTTATCAAt           >  1:599472/1‑151 (MQ=255)

                                                                                                                       aTTACGGCAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCGGGGTTTATCAAtt          <  1:143324/151‑1 (MQ=255)

                                                                                                                              cAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCGGGGTTTATCAATTGACGGc    >  1:611546/1‑150 (MQ=255)

                                                                                                                              cAACGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCa   <  1:32096/151‑1 (MQ=255)

                                                                                                                               aaCGGCGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCaa  <  1:361321/151‑1 (MQ=255)

TAGCACATCAATTAATGTCAACGCTGATGCGTCTACGGCACATCTCACCTTGCTACAGGCACTTTTTGATACAGTCTCCGCAGGCGAGACAACCAGTCTGTATATTGAGGTGAAGGATAATTACGGCAACGGTGTCCCCCAGCAGGAGGTAACCCTCAGCGTTTCACCAAGTGAAGGCGTGACCCCCAGTAATAACGCTATATATACTACCAACCACGACGGCAATTTTTACGCAAGCTTTACCGCTACAAAAGCCGGGGTTTATCAATTGACGGCAA  >  NC_000913/2048969‑2049246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A