Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A2 F50 I1 R1
|
170 |
39.5 |
1266514 |
99.2% |
1256381 |
150.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,227,340 |
(C)6→7 |
pseudogene (1270/1287 nt) |
mdtQ ← |
pseudogene; putative channel/filament proteins |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,227,334 | 1 | . | C | 96.7%
| 103.1
/ ‑2.9
| 30 | pseudogene (1276/1287 nt) | mdtQ | pseudogene; putative channel/filament proteins |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (15/14); total (16/14) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 |
TCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTA‑CCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGATGCCCGCGTTAAAGCGCGC > NC_000913/2227188‑2227478
|
tCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTA‑cccc > 2:91055/1‑151 (MQ=255)
aCCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTA‑cccccccg > 1:184437/1‑149 (MQ=255)
ccccTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTA‑cccccccgc > 2:451625/1‑148 (MQ=255)
gctgcGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCCGCCCAAc > 1:71531/1‑151 (MQ=255)
tgcGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCCGCCCAACGc > 1:403593/1‑151 (MQ=255)
cAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCCGCCCAACGCAcc > 1:628880/1‑149 (MQ=255)
gTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCCGCCCAACGCACCAGTg < 1:625749/151‑1 (MQ=255)
aCTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAg < 2:343192/151‑1 (MQ=255)
ttGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAgga < 1:257665/148‑2 (MQ=255)
tCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCAc > 1:433089/1‑151 (MQ=255)
ccccGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCAc < 1:407277/150‑1 (MQ=255)
ccGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGc < 1:142362/151‑1 (MQ=255)
ccGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGc > 1:93777/1‑151 (MQ=255)
ggTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACa < 2:306078/151‑1 (MQ=255)
taatCGCAAACTGTGCCATCGGGTTATCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGAGTTTGTA‑CCCCCCACCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCAt > 2:293528/1‑151 (MQ=255)
tCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTg > 1:619176/1‑151 (MQ=255)
cGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTg < 2:104941/150‑1 (MQ=255)
ccTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGc > 1:526901/1‑151 (MQ=255)
cATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGc > 2:485876/1‑150 (MQ=255)
gAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAgcgc > 1:378618/1‑151 (MQ=255)
gAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAgcgc > 2:53000/1‑151 (MQ=255)
ccACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCgggg < 1:4952/148‑1 (MQ=255)
ccACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGAtt < 2:85156/151‑1 (MQ=255)
tAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGc > 1:55348/1‑151 (MQ=255)
cGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTc < 2:503371/151‑1 (MQ=255)
aaTAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTg > 1:593798/1‑150 (MQ=255)
aGGCGCATATCATCAGCGTTTGTACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAatgat < 1:295625/151‑1 (MQ=255)
gcATATCATCAGCGTTTGTACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGATGccc < 2:218648/151‑1 (MQ=255)
atcatcAGCGTTTGTACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAAGCAACCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGATCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGATGCCCGCGt > 2:359886/1‑151 (MQ=255)
catcaGCGTTTGTACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGATGCCCGCGtt < 2:431639/150‑1 (MQ=255)
catcaGCGTTTGTACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGATGCCCGCGtt > 1:68066/1‑150 (MQ=255)
gTTTGTACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGATGCCCGCGTTAAAgcgcg < 1:464443/151‑1 (MQ=255)
tttGTACCCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGATGCCCGCGTTAAAgcgcgc > 1:470634/1‑151 (MQ=255)
|
TCAAACCCCTGCTGCGCCAGTAATAACGCGCACTCTTTGCCGATCCCCGAATCGGAGGCGGTAATAATCGCAACCTGTGCCATCGAGTTCTCCACTTAACGCTGAATAAACGTTAAGTATAGAAGGCGCATATCATCAGCGTTTGTA‑CCCCCCGCCCAACGCACCAGTGAGTTGAATGGAGGCATCCAGCCACTGCCCTTGCAATAACAGGCCATTGGCCCGCTCACGCAGCGCGGGGATTCTGGCTTCGCTGACGCGGGAACCAGCAATGATGCCCGCGTTAAAGCGCGC > NC_000913/2227188‑2227478
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
