Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F50 I1 R1 170 39.5 1266514 99.2% 1256381 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,358,589 A→G V86A (GTC→GCC)  rhmA ← 2‑keto‑3‑deoxy‑L‑rhamnonate aldolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,358,5890AG100.0% 101.9 / NA 30V86A (GTC→GCC) rhmA2‑keto‑3‑deoxy‑L‑rhamnonate aldolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/12);  total (18/12)

TCCCCAGCGCGCAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAA  >  NC_000913/2358447‑2358735
                                                                                                                                              |                                                                                                                                                  
tCCCCAGCGCGCAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGGCTTGttt                                                                                                                                             >  2:624229/1‑150 (MQ=255)
   ccAGCGCGCAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGGCTTGTTTAATc                                                                                                                                         <  1:437323/151‑1 (MQ=255)
            aGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGGCTTGTTTAATCAGCGGttt                                                                                                                                 >  1:371796/1‑150 (MQ=255)
              cccGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGGCTTGTTTAATCAGCGGTTTACt                                                                                                                              >  2:550556/1‑151 (MQ=255)
                     cacaCTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGGCTTGTTTAATCAGCGGTTTACTGCCTTcc                                                                                                                       >  2:363205/1‑151 (MQ=255)
                         cTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGGCTTGTTTAATCAGCGGTTTACTGCCTTCCAcc                                                                                                                    <  1:437929/150‑1 (MQ=255)
                              cccGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGGCTTGTTTAATCAGCGGTTTACTGCCTTCCACcggacgg                                                                                                              <  1:388240/151‑1 (MQ=255)
                              cccGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGACAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGGCTTGTTTAATCAGCGGTTTACTGCCTTCCACcggacgg                                                                                                              <  1:24036/151‑1 (MQ=255)
                                 gACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGGCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATc                                                                                                           >  1:46521/1‑151 (MQ=255)
                                    accacGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGGCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACg                                                                                                        >  2:560522/1‑151 (MQ=255)
                                      cacGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATGTCCAGGGCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACggg                                                                                                      <  2:142294/151‑1 (MQ=255)
                                        cGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGGCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGtt                                                                                                    >  1:271319/1‑151 (MQ=255)
                                                gTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGGCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCa                                                                                            <  1:253964/151‑1 (MQ=255)
                                                                      gACACCCCCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGGCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAg                                                                        <  2:210481/149‑1 (MQ=255)
                                                                         accaccTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGGCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCtgat                                                                   >  1:47273/1‑151 (MQ=255)
                                                                                            cGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGGCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAAt                                                   <  2:268749/148‑1 (MQ=255)
                                                                                            cGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGGCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAAt                                                   >  1:268756/1‑148 (MQ=255)
                                                                                             ggCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGGCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGgtg                                               >  2:257325/1‑151 (MQ=255)
                                                                                             ggCAGTATCGACCATCGCGATCAGTAGAGTTTGCGCGCCAATATCCAGGGCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCCCCTGTAGCTGATGATAAAGATCCTGAATGgt                                                >  2:309798/1‑150 (MQ=255)
                                                                                                 gTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGGCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTg                                           >  1:11648/1‑151 (MQ=255)
                                                                                                    tCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGGCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGgcg                                        >  1:372690/1‑151 (MQ=255)
                                                                                                        ccATCGGGATCAGTAGAGTTTGCGCGCCAATCTCCAGGGCTTGTTTAATCAGCGGTTTACTGCCTTCCCCCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTg                                    <  2:329931/151‑1 (MQ=255)
                                                                                                            cGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGGCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTcc                                >  2:295416/1‑151 (MQ=255)
                                                                                                             gggATCAGTAGAGTTTGCGCGCCAATATCCAGGGCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTccc                               >  1:199962/1‑151 (MQ=255)
                                                                                                               gATCAGTAGAGTTTGCGCGCCAATATCCAGGGCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCg                             <  1:463460/151‑1 (MQ=255)
                                                                                                                            tGCGCGCCAATATCCAGGGCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAAcc                >  2:360295/1‑151 (MQ=255)
                                                                                                                              cgcgcCAATATCCAGGGCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAAcc                >  1:72973/1‑149 (MQ=255)
                                                                                                                               gcgcCAATATCCAGGGCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGt             >  1:312253/1‑151 (MQ=255)
                                                                                                                                 gcCAATATCCAGGGCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTc            <  1:93571/150‑1 (MQ=255)
                                                                                                                                          cAGGGCTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGaa  <  2:3231/151‑1 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
TCCCCAGCGCGCAGCCCGTGCCACACTGGCCCCGACACCACGCTCACCGTAGGGAGGATAGCGCGTGGCAGACACCACCTGACGTGCCTGTTCGGCAGTATCGACCATCGGGATCAGTAGAGTTTGCGCGCCAATATCCAGGACTTGTTTAATCAGCGGTTTACTGCCTTCCACCGGACGGATCACGGGTTGGCTGGCATAGGGCGCTACCGCCTGTAGCTGATGATAAAGATCCTGAATGGTGTTTGGCGCGTGCTCCCCGTCAATCAGCAACCAGTCATAACCAGAA  >  NC_000913/2358447‑2358735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A