Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F50 I2 R1	121	82.0	2593086	97.7%	2533445	150.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,527,897	A→G	T318A (ACC→GCC)	yfeH →	putative inorganic ion transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,527,897	0	A	G	98.6%	254.4 / ‑3.8	71	T318A (ACC→GCC)	yfeH	putative inorganic ion transporter
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (34/36); total (34/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.62e-01

AAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCAT  >  NC_000913/2527753‑2528044

aaaaaGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAAc                                                                                                                                               <  2:503385/151‑1 (MQ=255)

aaaaaGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAAc                                                                                                                                               <  2:65706/151‑1 (MQ=255)

aaaaaGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAAc                                                                                                                                               >  2:724313/1‑151 (MQ=255)

  aaaGAGTCTGGCAAATGGCATCCCGATGGCCCACATTCTGTTCCCCACATCGGTGATCGCTATGATGGTGCTGCCCCTGATGATTTTCCATTAGATCCAATTGATGGTCTGTGCGATGCTGGCCCGTCGATACCAACGCCCGGCCGCACAg                                                                                                                                             <  1:116607/151‑1 (MQ=255)

     gagTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTa                                                                                                                                          <  2:852094/151‑1 (MQ=255)

         cTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAgg                                                                                                                                      >  2:174394/1‑151 (MQ=255)

         cTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAgg                                                                                                                                      >  1:1146576/1‑151 (MQ=255)

          tGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGgc                                                                                                                                     >  2:1223840/1‑151 (MQ=255)

           ggCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAgg                                                                                                                                      <  2:86605/149‑1 (MQ=255)

           ggCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAgg                                                                                                                                      <  1:532312/149‑1 (MQ=255)

             cAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCgca                                                                                                                                  <  2:835648/151‑1 (MQ=255)

              aaaTGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCgcag                                                                                                                                 <  2:719343/151‑1 (MQ=255)

               aaTGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCgcagc                                                                                                                                >  1:764749/1‑151 (MQ=255)

                    cATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGaa                                                                                                                           <  1:1130641/151‑1 (MQ=255)

                      tCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGaa                                                                                                                           >  1:1263216/1‑149 (MQ=255)

                        ccGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAag                                                                                                                         >  1:506575/1‑149 (MQ=255)

                         cGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAagcag                                                                                                                      <  2:1172578/151‑1 (MQ=255)

                         cGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAagc                                                                                                                        >  2:1073836/1‑149 (MQ=255)

                                   cATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATaa                                                                                                             >  2:561843/1‑150 (MQ=255)

                                    aTTCTGTTCCACACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATa                                                                                                              >  1:1007043/1‑148 (MQ=255)

                                           tCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTaaa                                                                                                     <  2:580611/150‑1 (MQ=255)

                                            ccccACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAg                                                                                                    <  2:288479/150‑1 (MQ=255)

                                              ccACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCgg                                                                                                 >  1:562482/1‑151 (MQ=255)

                                                acaTCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGAc                                                                                               <  1:7650/151‑1 (MQ=255)

                                                  aTCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGc                                                                                             >  1:114352/1‑151 (MQ=255)

                                                      gTGAGCGGTATGATGGTGATGCCCCTGGTGATTTTCCATCAGATCCACTTGATGGTCTGTGCGGTGCGGGCGCGACGACACAAAAGCCAGGCCGGAGAGATTCAGGAGAAGCAGGAAAGCAGCGCCGATAAAACTTAAAGCGGTCg                                                                                              >  1:1066049/1‑146 (MQ=255)

                                                        gATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAgg                                                                                       <  2:859732/151‑1 (MQ=255)

                                                         aTCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAggg                                                                                      >  1:501471/1‑151 (MQ=255)

                                                            ggTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCt                                                                                   >  2:571444/1‑151 (MQ=255)

                                                               atgatgGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGa                                                                                <  1:152814/151‑1 (MQ=255)

                                                                   tgGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGAcc                                                                              >  1:712579/1‑149 (MQ=255)

                                                                    ggTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGt                                                                           >  1:326738/1‑151 (MQ=255)

                                                                    ggTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAg                                                                            <  2:1027970/150‑1 (MQ=255)

                                                                     gTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGtt                                                                          >  2:1032548/1‑151 (MQ=255)

                                                                       gctgcCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGc                                                                        <  1:1184851/151‑1 (MQ=255)

                                                                         tgcCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGt                                                                      >  2:785779/1‑151 (MQ=255)

                                                                         tgcCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGt                                                                      >  2:1231309/1‑151 (MQ=255)

                                                                          gcCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTc                                                                     >  2:429974/1‑151 (MQ=255)

                                                                            cccTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTc                                                                     >  2:69284/1‑149 (MQ=255)

                                                                                 atgatTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCt                                                              <  1:803305/151‑1 (MQ=255)

                                                                                 atgatTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCt                                                              <  2:541787/151‑1 (MQ=255)

                                                                                  tgatTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTc                                                             >  1:543158/1‑151 (MQ=255)

                                                                                   gatTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCg                                                            <  1:269084/151‑1 (MQ=255)

                                                                                    atTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCgg                                                           <  2:944681/151‑1 (MQ=255)

                                                                                        tCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGttt                                                        <  2:818663/150‑1 (MQ=255)

                                                                                         ccATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGtttt                                                       <  2:424945/150‑1 (MQ=255)

                                                                                            tCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATc                                                   >  2:302695/1‑151 (MQ=255)

                                                                                             cAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATca                                                  <  1:724279/151‑1 (MQ=255)

                                                                                                aTCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATcagca                                               <  2:788443/151‑1 (MQ=255)

                                                                                                   cAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCg                                            >  2:987635/1‑151 (MQ=255)

                                                                                                   cAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCg                                            <  1:89362/151‑1 (MQ=255)

                                                                                                     aTTGATGGTCTGTGCGGTGCTGGCGCGTCGCTACAAACGCCAGGCCGAACAGTTACATGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGt                                           <  1:309214/150‑1 (MQ=255)

                                                                                                       tGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCTCCTCGGTTTTAATCAGCAGCGTGa                                         <  1:526056/150‑1 (MQ=255)

                                                                                                       tGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGAGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGAt                                        >  2:1141073/1‑151 (MQ=255)

                                                                                                         aTGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGAttt                                      >  1:298360/1‑151 (MQ=255)

                                                                                                         aTGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGAttt                                      >  2:214997/1‑151 (MQ=255)

                                                                                                             tCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCAt                                  >  1:27015/1‑151 (MQ=255)

                                                                                                                gtgCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCAtt                                 >  2:558317/1‑149 (MQ=255)

                                                                                                                gtgCGGTGCTGGCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCAtt                                 >  2:326069/1‑149 (MQ=255)

                                                                                                                          ggCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGa                       <  2:983064/149‑1 (MQ=255)

                                                                                                                          ggCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGa                       <  2:61928/149‑1 (MQ=255)

                                                                                                                          ggCGCGTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGa                       <  2:630943/149‑1 (MQ=255)

                                                                                                                              cgTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTcc                 >  1:264629/1‑151 (MQ=255)

                                                                                                                              cgTCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTcc                 <  2:564010/151‑1 (MQ=255)

                                                                                                                                tCGATACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCt               >  2:693713/1‑151 (MQ=255)

                                                                                                                                   aTACAAACGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGcc            <  2:1122554/151‑1 (MQ=255)

                                                                                                                                       aaaCGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGa        <  1:221200/151‑1 (MQ=255)

                                                                                                                                        aaCGCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGaa       <  2:246565/151‑1 (MQ=255)

                                                                                                                                           gCCAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACt     <  1:1123827/150‑1 (MQ=255)

                                                                                                                                            ccAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCa   <  2:441276/151‑1 (MQ=255)

                                                                                                                                             cAGGCCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCAt  >  2:171661/1‑151 (MQ=255)

AAAAAGAGTCTGGCAAATGGCATCCCGATGGCAAACATTCTGTTCCCCACATCGGTGATCGGTATGATGGTGCTGCCCCTGATGATTTTCCATCAGATCCAATTGATGGTCTGTGCGGTGCTGGCGCGTCGATACAAACGCCAGACCGAACAGTTACAGGCGCAGCAGGAAAGCAGCGCCGATAAAGCTTAAAGCGGACGCTTCAGGGGCTGGACCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCAT  >  NC_000913/2527753‑2528044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A