Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A13 F103 I0 R1
|
1593 |
59.4 |
2889406 |
94.0% |
2716041 |
140.5 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_002947 |
5,986,861 |
C→T |
100% |
intergenic (+141/‑58) |
PP_5245 → / → kefB‑III |
AraC family transcriptional regulator/glutathione‑regulated potassium/H+ antiporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_002947 | 5,986,861 | 0 | C | T | 90.2%
| 133.5
/ 5.8
| 52 | intergenic (+141/‑58) | PP_5245/kefB‑III | AraC family transcriptional regulator/glutathione‑regulated potassium/H+ antiporter |
| Reads supporting (aligned to +/- strand): ref base C (4/1); new base T (25/21); total (29/23) |
| Fisher's exact test for biased strand distribution p-value = 3.75e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.34e-01 |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
TTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCGTCCCCCTGGCCAAGCGCCTG > NC_002947/5986717‑5987002
|
ttAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGaa < 2:190991/149‑1 (MQ=255)
ccTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTc < 3:3762/149‑1 (MQ=255)
ttcttcGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCa > 7:64211/1‑148 (MQ=255)
tcttcGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGCGGCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCa > 1:281264/1‑148 (MQ=255)
gcgGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGtt < 6:115686/149‑1 (MQ=255)
gcgGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGAACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGtt < 7:193303/149‑1 (MQ=255)
ccGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTc > 1:237828/1‑127 (MQ=255)
ccGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTc < 2:237828/127‑1 (MQ=255)
tCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCg > 8:52090/1‑149 (MQ=255)
tCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCg > 3:124148/1‑149 (MQ=255)
ccACAGAGATAGCACATGCCTTGAGCCCAGGTAAGC‑GCCGTCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCg < 3:262419/147‑1 (MQ=255)
ccACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCgg < 4:166151/148‑1 (MQ=255)
ccACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGc > 2:112171/1‑149 (MQ=255)
acaGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAg > 3:347603/1‑149 (MQ=255)
tAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATgg < 4:347603/149‑1 (MQ=255)
gCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGa < 8:64211/148‑1 (MQ=255)
gCACAGGCCTTGACCCCAGGTAAGC‑GCCGGGACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTCCCCCGGCAGCTCATGGa < 3:277962/148‑1 (MQ=255)
acaGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTa > 6:244363/1‑149 (MQ=255)
acaGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTa < 2:217976/149‑1 (MQ=255)
gCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAg > 7:279848/1‑149 (MQ=255)
ttGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCACTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGcc > 6:201054/1‑148 (MQ=255)
aGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCcaca > 3:69927/1‑149 (MQ=255)
aCCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTc < 6:99863/149‑1 (MQ=255)
gATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTg > 2:89679/1‑148 (MQ=255)
aTTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGCCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGc > 2:292793/1‑148 (MQ=255)
ttttaaCTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGc < 7:340674/132‑1 (MQ=255)
ttGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCACGGCAGATCATGGAGTACGAGCCAATGCAACACGAAGGc > 8:340674/1‑137 (MQ=255)
gTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCaaa < 5:244363/148‑1 (MQ=255)
ctctGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACcgcg > 8:128448/1‑149 (MQ=255)
ctctGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACcgcg > 1:309877/1‑149 (MQ=255)
gacaATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTcc > 5:164483/1‑149 (MQ=255)
gacaATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCATCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAAt > 8:236933/1‑108 (MQ=255)
gacaATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCATCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAAt < 7:236933/108‑1 (MQ=255)
aaTTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTGGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTc > 6:265391/1‑145 (MQ=255)
aaTTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTTGGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTc < 5:265391/145‑1 (MQ=255)
aaTTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCATAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGc > 5:168634/1‑149 (MQ=255)
tttttCTTCCGGTCTGATGATTGTCTGACGCCACTCTACCCCTTAGAATTGTCCGTCATACTGTTCGAAGGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCtt < 6:168634/149‑1 (MQ=255)
ttcCCGTCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACAGCGGTGATCTTCCTGCTTgccgcc > 6:325248/1‑149 (MQ=255)
ttcCCGTCAGATGATTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTgccgcc > 3:321719/1‑149 (MQ=255)
tCAGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTc < 2:309877/149‑1 (MQ=255)
aGATGATTGTCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGc < 8:279848/149‑1 (MQ=255)
aGATGATTGCCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGc < 1:292793/149‑1 (MQ=255)
gatgatTGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGcc > 4:319994/1‑149 (MQ=255)
atgatTGTCTGACGCCACACTACCCCTTAGAATTGTACGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTATGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCg > 7:228344/1‑149 (MQ=255)
ttGACTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCACTGCAGCTCATGGAGTACGACCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTACTCCTTGACGCCGTCCTCGCAGTccc > 6:217862/1‑149 (MQ=255)
tCTGACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCGTCCCCCt > 8:285333/1‑149 (MQ=255)
gACGCCACACTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCGTCCCCCTGGc > 2:56024/1‑149 (MQ=255)
aCGCCACACTACCCCTGAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCGTCCCCCTGGcc < 5:217862/149‑1 (MQ=255)
acTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGc < 6:261194/65‑1 (MQ=255)
acTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGc > 5:261194/1‑65 (MQ=255)
cTACCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCGTCCCCCTGGCCAAGCGCCt < 6:164483/149‑1 (MQ=255)
aCCCCTTAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCGTCCCCCTGGCCAAGCGCCTg > 5:160986/1‑148 (MQ=255)
|
TTAGCGCTGCCTTCTTCGCGGGCGCGCCCGCTCCCACAGAGATAGCACAGGCCTTGAGCCCAGGTAAGC‑GCCGGCACCGATTGTCACTCTGGACAGGACAATTTTTCTTCCCGTCAGATGATTGTCTGACGCCACACTACCCCTCAGAATTGTCCGTCAAACTGTTCGAACGCTGCCCCGGCAGCTCATGGAGTACGAGCCAATGCCACACGAAGGCAGCCTTCTGCAAACCGCGGTGATCTTCCTGCTTGCCGCCGTCCTCGCCGTCCCCCTGGCCAAGCGCCTG > NC_002947/5986717‑5987002
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
