Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4926 296.4 4517418 96.8% 4372860 264.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 619,917 3 bp→TCG intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583619,9170AT100.0% 614.7 / NA 259intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (122/137);  total (122/137)
*CP042583619,9180GC99.2% 610.5 / NA 259intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base C (120/136);  minor base T (2/0);  total (122/137)
Fisher's exact test for biased strand distribution p-value = 2.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583619,9190CG100.0% 616.9 / NA 259intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (122/137);  total (122/137)

Only 100 of 259 total aligned reads displayed.

AGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTAAGCGTACACGGCGCTAACCGTCTGGGCGGCAACAGCCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCAGCGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAG  >  CP042583/619637‑620195
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                    
aGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTacac                                                                                                                                                                                                                                                                                <  1:468905/289‑1 (MQ=255)
aGGTCGCGGCTGTGATGCTCCGTGGGGGCCACACGCGAAACTGACACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTacac                                                                                                                                                                                                                                                                                <  2:1146515/289‑1 (MQ=255)
aGGTCGCGGCGGGGAGGGTCCGTGGGGGCAAAACGGGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGACGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTacac                                                                                                                                                                                                                                                                                <  2:1985612/289‑1 (MQ=255)
  gTCGGGGCTGGGATGGTCCGAGGGGGCCAAACGCGAAGCTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGGCTGCCGGGGATCCTGGGGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTacac                                                                                                                                                                                                                                                                                <  2:1712721/287‑1 (MQ=255)
                    cGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGggcgg                                                                                                                                                                                                                                                            >  2:1405453/1‑289 (MQ=255)
                      tGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCa                                                                                                                                                                                                                                                          >  1:1572540/1‑289 (MQ=255)
                      tGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGGTTATCGGTACACGGGGCTAACCGTCTGGGCGGCa                                                                                                                                                                                                                                                          >  2:1514290/1‑289 (MQ=255)
                       gggggCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCa                                                                                                                                                                                                                                                          <  1:1223409/288‑1 (MQ=255)
                                acGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAActcgctgct                                                                                                                                                                                                                                                <  2:1421819/289‑9 (MQ=255)
                                acGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAActcgct                                                                                                                                                                                                                                                   >  2:257359/1‑281 (MQ=255)
                                   cgAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAActcgctg                                                                                                                                                                                                                                                  >  1:948002/1‑278 (MQ=255)
                                   cgAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAActcgctg                                                                                                                                                                                                                                                  <  2:948002/284‑7 (MQ=255)
                                        ctgaaactCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCtgg                                                                                                                                                                                                                                        <  1:1675904/289‑1 (MQ=255)
                                                  ctCACCGGTGTAAAGACGTCTGCGAATCCACTCTGCCGGGGACCCTGGAGCTTTCCGTTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTgg                                                                                                                                                                                                                              <  2:1098889/288‑1 (MQ=255)
                                                  aTCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTgg                                                                                                                                                                                                                              >  1:2186610/1‑289 (MQ=255)
                                                               agaagTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAgg                                                                                                                                                                                                                  >  1:854389/1‑288 (MQ=255)
                                                                    ttCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGtctgca                                                                                                                                                                                                            >  1:917614/1‑289 (MQ=255)
                                                                    ttCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGtctgca                                                                                                                                                                                                            >  2:1079915/1‑289 (MQ=255)
                                                                     tctcGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGtctgc                                                                                                                                                                                                             <  1:1405453/287‑1 (MQ=255)
                                                                           aTCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCaa                                                                                                                                                                                                     <  1:567227/289‑1 (MQ=255)
                                                                           aTCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCaa                                                                                                                                                                                                     <  2:917614/289‑1 (MQ=255)
                                                                                 tCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCt                                                                                                                                                                                               >  2:1282934/1‑289 (MQ=255)
                                                                                          tATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGa                                                                                                                                                                                       <  1:1651923/288‑1 (MQ=255)
                                                                                           agccTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGc                                                                                                                                                                                     <  1:1245110/287‑1 (MQ=255)
                                                                                           aTCCTGGAGCTTTCCCGTACCTTCGCTCCCGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGc                                                                                                                                                                                     <  2:683607/289‑1 (MQ=255)
                                                                                             ccTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCa                                                                                                                                                                                    <  1:214/288‑1 (MQ=255)
                                                                                                  aGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGt                                                                                                                                                                                                                 <  1:788800/254‑1 (MQ=255)
                                                                                                     tttCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCAc                                                                                                                                                                            <  1:1039760/288‑1 (MQ=255)
                                                                                                      ttCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCtgg                                                                                                                                                                                                                                        <  1:1069028/227‑1 (MQ=255)
                                                                                                      ttCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGAATGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGCCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCGGCAAGAGTCTATCGCAGAGCCGGgcgcccg                                                                                                                                                                           >  2:1741309/1‑285 (MQ=255)
                                                                                                      ttCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTCGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACt                                                                                                                                                                           >  1:1295701/1‑288 (MQ=255)
                                                                                                       tCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTgc                                                                                                                                                                         >  1:1893213/1‑289 (MQ=255)
                                                                                                       tCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCACCTGCAAGAGTCTATCGCCGAGCa                                                                                                                                                                                    >  1:495795/1‑278 (MQ=255)
                                                                                                        cccGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTgcg                                                                                                                                                                        <  1:1732706/289‑1 (MQ=255)
                                                                                                        cccGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTgcg                                                                                                                                                                        <  2:47268/289‑1 (MQ=255)
                                                                                                             tccgtCGCTGACGTGGGGCCGGGGAAAGAGCCGATTCCGGGTATCCCTACGTGTAACTACATGATGGGCGGTATGCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAGGGCGAAGATGTGGTTGTGCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATg                                                                                                                                                                   <  2:2126518/285‑1 (MQ=255)
                                                                                                                  cGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAg                                                                                                                                                               >  1:1001522/1‑288 (MQ=255)
                                                                                                                  cGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGc                                                                                                                                                              <  2:341986/289‑1 (MQ=255)
                                                                                                                       aCGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTc                                                                                                                                                         <  1:762354/289‑1 (MQ=255)
                                                                                                                         gTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTg                                                                                                                                                       >  1:1198001/1‑289 (MQ=255)
                                                                                                                          ccGAGCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGGGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTg                                                                                                                                                       <  2:580133/287‑1 (MQ=255)
                                                                                                                                 ggTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGGGAAATCGCTTGTGTATCGGTCCACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCGTGGGTGGCGCGGCAGGTCTGCATCCGCACGAGTCGAGCGCCGGGCAGGGCGCACGGCGCGGGGCAACGGGGTCGGATGTTGaa                                                                                                                                               >  2:762354/1‑289 (MQ=255)
                                                                                                                                   tGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAg                                                                                                                                              <  1:554134/288‑1 (MQ=255)
                                                                                                                                   tGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAg                                                                                                                                              <  2:791409/288‑1 (MQ=255)
                                                                                                                                   tGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAg                                                                                                                                              <  1:2168373/288‑1 (MQ=255)
                                                                                                                                   tGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACAGGATGGGCGGGATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTGGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAg                                                                                                                                              <  2:1602814/288‑1 (MQ=255)
                                                                                                                                          gCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTg                                                                                                                                      >  2:2178591/1‑289 (MQ=255)
                                                                                                                                             gATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTcg                                                                                                                                                                                                                           >  2:1201856/1‑201 (MQ=255)
                                                                                                                                             gATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAAACGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGa                                                                                                                                    >  1:253638/1‑288 (MQ=255)
                                                                                                                                                     ttATCCCGACCTGTAACTACCTGATGGGTGGGAGTCCGACCAAAGTTACCGGTCAGGCACTGGCTGGGAAGGGGAAAGGCGAAGATGTGGTTGTTACGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGa                                                                                                                            <  2:860610/288‑1 (MQ=255)
                                                                                                                                                        tCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTcgcgcg                                                                                                                                                                                                                       >  2:1575484/1‑194 (MQ=255)
                                                                                                                                                        tCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGt                                                                                                                                                                                                                 >  1:809055/1‑200 (MQ=255)
                                                                                                                                                              ccTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGCTCGCCTGAACCGc                                                                                                                       >  2:1917857/1‑284 (MQ=255)
                                                                                                                                                                 gTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAAct                                                                                                                                                                                                                                                       <  1:2236695/153‑2 (MQ=255)
                                                                                                                                                                  tCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAg                                                                                                                                              <  2:612020/257‑1 (MQ=255)
                                                                                                                                                                  tCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAg                                                                                                                                              >  1:612020/1‑257 (MQ=255)
                                                                                                                                                                   aaCTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGGGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACaat                                                                                                              <  1:1453982/287‑1 (MQ=255)
                                                                                                                                                                       aaaggtgggggggTATTCCAGCCCAAGTTGCCGGTCAGGCACTGACTGTGAATGGGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCg                                                                                                         <  2:1068482/282‑1 (MQ=255)
                                                                                                                                                                        cATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATAGCCTGAACCGCTGGAACAATAATCGt                                                                                                        >  1:1531001/1‑289 (MQ=255)
                                                                                                                                                                         atgatgGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTa                                                                                                       >  2:1309749/1‑289 (MQ=255)
                                                                                                                                                                          tgatgGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTaa                                                                                                      <  1:868920/289‑1 (MQ=255)
                                                                                                                                                                          tgatgGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTaa                                                                                                      <  2:559217/289‑1 (MQ=255)
                                                                                                                                                                            atgGGCGGTATTCCGACCAAAGTTACCGGTCAGGCGCTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGGTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACg                                                                                                    >  2:409226/1‑289 (MQ=255)
                                                                                                                                                                            atgGGCGGTATTCCGAACAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTATGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGGCTATCGGCGAGCAGGGCGCAGTGCGCCATGCCAGGCACGCTGGTGTTGAAGCGTGTCTGGATCGGCTGAACCGCAGGGAAactaatgcttaag                                                                                                    >  2:1323233/1‑282 (MQ=255)
                                                                                                                                                                                gCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGa                                                                                                >  1:309009/1‑289 (MQ=255)
                                                                                                                                                                                gCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGa                                                                                                >  2:465136/1‑289 (MQ=255)
                                                                                                                                                                                 cGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAATGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGa                                                                                                <  1:1741309/288‑1 (MQ=255)
                                                                                                                                                                                    tATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGa                                                                                                                                    <  1:2087542/249‑1 (MQ=255)
                                                                                                                                                                                    tATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGa                                                                                                                                    >  2:2087542/1‑249 (MQ=255)
                                                                                                                                                                                    tATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGa                                                                                                                                    <  1:1144637/249‑1 (MQ=255)
                                                                                                                                                                                           aCCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCtgg                                                                                                                                                                                                                                        >  2:1162847/1‑142 (MQ=255)
                                                                                                                                                                                           aCCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTgg                                                                                     >  2:1037048/1‑289 (MQ=255)
                                                                                                                                                                                              aaaGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGa                                                                                  >  1:386063/1‑289 (MQ=255)
                                                                                                                                                                                               aggTTACGGGTCAGGCACGGAGTGTGAATGAGAAGGGCGAGGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCGGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGAt                                                                                 <  2:1893213/287‑1 (MQ=255)
                                                                                                                                                                                                          cAGGCACTGACTGTGAATGAGGAAGGCGAAGATGTGGTGTTTCCGTGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTCTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAgcg                                                                       <  2:1852165/288‑1 (MQ=255)
                                                                                                                                                                                                          cAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGa                                                                                                >  1:133809/1‑263 (MQ=255)
                                                                                                                                                                                                           aGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTgcg                                                                                                                                                                        <  1:99794/190‑1 (MQ=255)
                                                                                                                                                                                                               actgactgTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCa                                                                  <  1:1309749/288‑1 (MQ=255)
                                                                                                                                                                                                                   actgTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTgg                                                                                                                                     <  2:2142651/217‑1 (MQ=255)
                                                                                                                                                                                                                      gtgGATGAGGAAGGCGATGTGGGGGTTGTTCTGGGACGGGTGGCCGTGGGTGGAATCGCTTGTGGATCGGTACACGGCGCTAGCCGTCTGGGCGGCAACTCGCTGCGGGACCTGGTGGTCTTTGGTCGCGCGGCAGGGCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAGCAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTa                                                          <  2:1664075/289‑1 (MQ=255)
                                                                                                                                                                                                                       tgAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAAc                                                                                                     >  2:2218039/1‑245 (MQ=255)
                                                                                                                                                                                                                       tgAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGAGTTTGAAGCGTCTCTGGATCGCCTTAACCGCTGGCACAATAATCGTAAAGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGCATGAAt                                                         >  1:722053/1‑289 (MQ=255)
                                                                                                                                                                                                                         aaTGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATgc                                                       <  1:663087/289‑1 (MQ=255)
                                                                                                                                                                                                                              gaAAGGAGAAGAGGGGGGGGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAAcc                                                                                                                                                                                                                                                                      >  1:245273/1‑77 (MQ=255)
                                                                                                                                                                                                                               aaaGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTgcg                                                                                                                                                                        >  1:1619893/1‑170 (MQ=255)
                                                                                                                                                                                                                               aaaGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTGTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAATAATGTATgcagcaga                                                 >  1:1823685/1‑287 (MQ=255)
                                                                                                                                                                                                                                 aGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATa                                                 >  2:702736/1‑287 (MQ=255)
                                                                                                                                                                                                                                   gCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTc                                                                                                                                                         <  1:191965/181‑1 (MQ=255)
                                                                                                                                                                                                                                   gCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACtt                                             >  1:1091357/1‑289 (MQ=255)
                                                                                                                                                                                                                                   gCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACt                                              >  1:1973813/1‑288 (MQ=255)
                                                                                                                                                                                                                                      aaGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTct                                           <  1:1770718/288‑2 (MQ=255)
                                                                                                                                                                                                                                       aGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCCGGAACAATAATCGTAACGGTGAAGATCCGGTCGCGATCCGTAAAGCGCTGCAAGAATGTATGAAGCATAACTTctcg                                         >  1:1064747/1‑286 (MQ=255)
                                                                                                                                                                                                                                          tgtgGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTctcggt                                       <  1:449222/288‑6 (MQ=255)
                                                                                                                                                                                                                                             ggTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTgg                                                                                                                    >  2:721291/1‑208 (MQ=255)
                                                                                                                                                                                                                                                   tCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGGAAAGCGCTGCAAGACTGTATGCAGCATAAATTCCCGGTTGTCCGTGaa                             >  2:1628514/1‑289 (MQ=255)
                                                                                                                                                                                                                                                      gggACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGt                          >  1:108566/1‑289 (MQ=255)
                                                                                                                                                                                                                                                      gggACTGTTTGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGt                          >  1:1164294/1‑289 (MQ=255)
                                                                                                                                                                                                                                                             tttGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATc                                                                                           >  1:1831006/1‑217 (MQ=255)
                                                                                                                                                                                                                                                             tttGCCGTTGGTGAAATCGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTa                                                          >  2:1769351/1‑250 (MQ=255)
                                                                                                                                                                                                                                                                              cGCTTGTGTATCGGTACACGGCGCTAACCGTCTGGGCGGCAACTCGCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCTCGGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAg  <  2:1216310/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                    
AGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGTCACTACATGATGGGCGGTATTCCGACCAAAGTTACCGGTCAGGCACTGACTGTGAATGAGAAAGGCGAAGATGTGGTTGTTCCGGGACTGTTTGCCGTTGGTGAAATCGCTTGTGTAAGCGTACACGGCGCTAACCGTCTGGGCGGCAACAGCCTGCTGGACCTGGTGGTCTTTGGTCGCGCGGCAGGTCTGCATCTGCAAGAGTCTATCGCCGAGCAGGGCGCACTGCGCGATGCCAGCGAGTCTGATGTTGAAGCGTCTCTGGATCGCCTGAACCGCTGGAACAATAATCGTAACGGTGAAGATCCGGTGGCGATCCGTAAAGCGCTGCAAGAATGTATGCAGCATAACTTCAGCGTCTTCCGTGAAGGTGATGCGATGGCGAAAGGGCTTGAG  >  CP042583/619637‑620195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A