Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A0 F0 I1 R1 4926 296.4 4517418 96.8% 4372860 264.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,927,503 3 bp→TCA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,927,5030AT98.6% 822.4 / NA 359intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (2/3);  new base T (174/175);  total (180/179)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,927,5040GC100.0% 851.8 / NA 359intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (180/179);  total (180/179)
*CP0425831,927,5050TA99.4% 845.3 / NA 359intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/1);  major base A (179/176);  minor base C (1/1);  total (180/179)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 359 total aligned reads displayed.

GGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGAGCATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCAGTACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGGCTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGT  >  CP042583/1927222‑1927781
                                                                                                                                                                                                                                                                                         |||                                                                                                                                                                                                                                                                                    
       cAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACAttt                                                                                                                                                                                                                                                                          >  1:431362/1‑289 (MQ=255)
           gTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGcc                                                                                                                                                                                                                                                                       >  2:582905/1‑288 (MQ=255)
           gTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGcc                                                                                                                                                                                                                                                                       <  1:1164238/288‑1 (MQ=255)
                  cTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGcgcg                                                                                                                                                                                                                                                                >  2:894501/1‑288 (MQ=255)
                       caagcaCAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAAt                                                                                                                                                                                                                                                          >  1:798194/1‑289 (MQ=255)
                       caagcaCAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCAGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGATGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGAAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAAt                                                                                                                                                                                                                                                          >  1:1702367/1‑289 (MQ=255)
                        aagcaCAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGcgacga                                                                                                                                                                                                                                                            <  1:1637192/286‑1 (MQ=255)
                             caATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCgg                                                                                                                                                                                                                                                    <  2:1282774/289‑1 (MQ=255)
                             caATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCgg                                                                                                                                                                                                                                                    <  1:402845/289‑1 (MQ=255)
                               atatAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGcc                                                                                                                                                                                                                                                                       >  2:298567/1‑268 (MQ=255)
                               atatAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGaa                                                                                                                                                                                                                                                  >  1:1278173/1‑289 (MQ=255)
                                      gACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGa                                                                                                                                                                                                                                           >  1:1133829/1‑289 (MQ=255)
                                        ccAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGa                                                                                                                                                                                                                                           <  2:431362/287‑1 (MQ=255)
                                        ccAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAAt                                                                                                                                                                                                                                         >  1:186540/1‑289 (MQ=255)
                                        ccAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCCCCTCAACCTGTACATTTGCCCTGCCCGACGAATAACCGGAAGTGCTGAAt                                                                                                                                                                                                                                         >  2:1811524/1‑289 (MQ=255)
                                          aGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGAAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGCAGGGCTGAAAGt                                                                                                                                                                                                                                       >  2:2187556/1‑289 (MQ=255)
                                           ggTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGACAACCAGGAAGTGCTGAATGTc                                                                                                                                                                                                                                      >  1:1483052/1‑289 (MQ=255)
                                            gTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGcgac                                                                                                                                                                                                                                                              <  2:1203845/264‑1 (MQ=255)
                                            gTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCg                                                                                                                                                                                                                                     >  2:1887339/1‑289 (MQ=255)
                                                  ttctttcACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGacaaca                                                                                                                                                                                                                               <  2:1953053/289‑1 (MQ=255)
                                                        cACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCt                                                                                                                                                                                                                         <  1:918980/289‑1 (MQ=255)
                                                                            cAAACATCCGAATTTTGATAAAGATTTAATTAAAGTGCTCAACCAAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGAAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTg                                                                                                                                                                                                     <  2:113066/289‑1 (MQ=255)
                                                                             aaCCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCg                                                                                                                                                                                                          <  2:186472/283‑1 (MQ=255)
                                                                                  tCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCTCGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAAc                                                                                                                                                                                                >  2:1470291/1‑288 (MQ=255)
                                                                                  tCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGa                                                                                                                                                                                                                                    <  1:1233941/252‑1 (MQ=255)
                                                                                                aaGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGgcg                                                                                                                                                                                 <  2:1337508/289‑1 (MQ=255)
                                                                                                            aaGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGTATTGCGCTATCATATCGAACTGGCAACCCGCCGGCTGTGGCCCACGCgggtaca                                                                                                                                                                     >  2:1035542/1‑285 (MQ=255)
                                                                                                                 cTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCa                                                                                                                                                                >  2:2144820/1‑289 (MQ=255)
                                                                                                                 cTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCa                                                                                                                                                                >  1:246278/1‑289 (MQ=255)
                                                                                                                  tCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCaa                                                                                                                                                               <  1:1007770/289‑1 (MQ=255)
                                                                                                                  tCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCa                                                                                                                                                                <  1:750876/288‑1 (MQ=255)
                                                                                                                   cAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCaaa                                                                                                                                                              >  1:1419497/1‑289 (MQ=255)
                                                                                                                      cGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCAGCTGGCTGTGGCGCAAGCTGGACGAAGGCaaa                                                                                                                                                              >  2:139278/1‑286 (MQ=255)
                                                                                                                         aaTGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGtt                                                                                                                                                         <  1:2144820/288‑1 (MQ=255)
                                                                                                                                  cGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGaaa                                                                                                                                               >  2:1481192/1‑289 (MQ=255)
                                                                                                                                       tACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAAcc                                                                                                                                             >  1:1532841/1‑286 (MQ=255)
                                                                                                                                       tACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAAcc                                                                                                                                             >  1:151005/1‑286 (MQ=255)
                                                                                                                                        aCAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAAccgct                                                                                                                                          <  2:533335/288‑1 (MQ=255)
                                                                                                                                         cAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGc                                                                                                                                                                                                                                              >  2:97409/1‑187 (MQ=255)
                                                                                                                                         cAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGc                                                                                                                                                                                                                                              <  1:97409/187‑1 (MQ=255)
                                                                                                                                              gCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAAccgctgccgctg                                                                                                                                   >  1:2119996/1‑289 (MQ=255)
                                                                                                                                               cTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGGGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGa                                                                                                                                  >  1:1486885/1‑289 (MQ=255)
                                                                                                                                               cTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGcgacga                                                                                                                                                                                                                                                            >  2:2059489/1‑167 (MQ=255)
                                                                                                                                               cTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAAccc                                                                                                                                                                                              <  2:152923/229‑1 (MQ=255)
                                                                                                                                               cTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCgct                                                                                                                                                                                           <  2:1687062/232‑1 (MQ=255)
                                                                                                                                               cTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAACACGCTGCCGCTGa                                                                                                                                  >  1:2079508/1‑289 (MQ=255)
                                                                                                                                               cTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAAccgct                                                                                                                                          >  2:402840/1‑281 (MQ=255)
                                                                                                                                               cTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGa                                                                                                                                  >  2:1499463/1‑289 (MQ=255)
                                                                                                                                               cTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGa                                                                                                                                  >  1:1713625/1‑289 (MQ=255)
                                                                                                                                               cTCAGCAAAGAAAAATGCTACGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGa                                                                                                                                                                                                                                    <  2:258753/191‑1 (MQ=255)
                                                                                                                                                 cAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTg                                                                                                                                                                                                                                                                         <  1:1782160/152‑1 (MQ=255)
                                                                                                                                                 cAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGaaa                                                                                                                                <  1:524006/289‑1 (MQ=255)
                                                                                                                                                      aaGAGAAATGCTGCGGCGTACCGCTACTCGCCAACGGCTTTAACGATAAAGCACGCAAACAGGAAATTACGAATGTAGCGTCGATCAGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAGCCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGt                                                                                                                           <  2:1779132/289‑1 (MQ=255)
                                                                                                                                                      aaGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGt                                                                                                                           <  2:610351/289‑1 (MQ=255)
                                                                                                                                                      aaGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGt                                                                                                                           <  1:773348/289‑1 (MQ=255)
                                                                                                                                                      aaGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGt                                                                                                                           <  1:1774063/289‑1 (MQ=255)
                                                                                                                                                       aGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGtt                                                                                                                          >  1:84843/1‑289 (MQ=255)
                                                                                                                                                         aaaaaTGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTa                                                                                                                        >  2:557350/1‑289 (MQ=255)
                                                                                                                                                              tgctgcGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATcac                                                                                                                    >  1:26279/1‑288 (MQ=255)
                                                                                                                                                              tgctgcGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGAGCATATCGAACTGGCAACCCGCTGGCTGTGGCGGCAGCTGGACGAAGGCAAAAAGTTACCGCTGAAACCGCTGCCGCTAAAAGTGGTTTATccca                                                                                                                   >  1:1029513/1‑289 (MQ=255)
                                                                                                                                                                                       aCGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATggg                                                                                          <  2:84843/289‑1 (MQ=255)
                                                                                                                                                                                            tttACCGATAAAGCTCGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTgg                                                                                      <  1:1470291/288‑1 (MQ=255)
                                                                                                                                                                                            tttACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTgg                                                                                      <  1:303257/288‑1 (MQ=255)
                                                                                                                                                                                            tttACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGa                                                                                     <  1:2245506/289‑1 (MQ=255)
                                                                                                                                                                                                   aTAAAGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTa                                                                              <  2:493580/289‑1 (MQ=255)
                                                                                                                                                                                                     aaaGCACGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTac                                                                             >  1:504799/1‑288 (MQ=255)
                                                                                                                                                                                                           cGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGTCAACCCGCTGGCTGTGGCGCAAGCTGGa                                                                                                                                                                        >  1:108624/1‑191 (MQ=255)
                                                                                                                                                                                                           cGCAAACAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGa                                                                                                                                                                        <  2:108624/191‑1 (MQ=255)
                                                                                                                                                                                                                aCAGGCAATTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTg                                                                 <  1:557350/289‑1 (MQ=255)
                                                                                                                                                                                                                      aaTTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGt                                                           >  2:517686/1‑289 (MQ=255)
                                                                                                                                                                                                                      aaTTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCCCTACACCCTGGAGCTGTTGCGt                                                           >  1:997378/1‑289 (MQ=255)
                                                                                                                                                                                                                       aTTACGAATGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTa                                                          <  1:638400/289‑1 (MQ=255)
                                                                                                                                                                                                                              aTGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATcc                                                    <  2:671468/288‑1 (MQ=255)
                                                                                                                                                                                                                              aTGTAGAGTCGATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATcc                                                    <  2:91125/288‑1 (MQ=255)
                                                                                                                                                                                                                                    agtcgaTCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAAccgct                                                                                                                                          <  1:1966488/191‑1 (MQ=255)
                                                                                                                                                                                                                                    agtcgaTCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGa                                                                                     <  1:140464/244‑1 (MQ=255)
                                                                                                                                                                                                                                     gtcgaTCCGCGAAGCGGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGGCt                                             <  2:1419497/284‑1 (MQ=255)
                                                                                                                                                                                                                                      tcgaTCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACAccc                                                                         >  2:1800604/4‑259 (MQ=255)
                                                                                                                                                                                                                                         aTCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGcc                                                                                                                                                                                                                                                                       >  1:2187001/1‑66 (MQ=255)
                                                                                                                                                                                                                                         aTCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGa                                                                                                                                                                                                                                    >  2:445653/1‑101 (MQ=255)
                                                                                                                                                                                                                                         aTCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATc                                                                                                                      >  1:2010625/1‑211 (MQ=255)
                                                                                                                                                                                                                                         aTCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATc                                                                                                                      <  2:2010625/211‑1 (MQ=255)
                                                                                                                                                                                                                                         aTCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGaaaaa                                                                                               >  2:765771/1‑234 (MQ=255)
                                                                                                                                                                                                                                         aTCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCAGATGGAAAAAATGGGCTGGGCGCTCTACACCCTGGATCTGTTGCGTAACATCCCGGGGCTTggtt                                        >  2:203853/1‑286 (MQ=255)
                                                                                                                                                                                                                                            cggggaGCTGTGGAAGTAAAAGCCATTCCGGTGATTGCCACCTCCACAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGGCTTGAGTTaa                                     <  2:2249533/284‑1 (MQ=255)
                                                                                                                                                                                                                                            cgcgAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGGCTTGAGTTaa                                     <  2:2099924/289‑1 (MQ=255)
                                                                                                                                                                                                                                            cgcgAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGGCTTGAGTTaa                                     <  1:1916547/289‑1 (MQ=255)
                                                                                                                                                                                                                                                    tgtgGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGGCTTGAGTTaa                                     <  1:2055431/281‑1 (MQ=255)
                                                                                                                                                                                                                                                    tgtgGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGGCTTGAGTTAACGGTGc                               <  2:1264640/287‑1 (MQ=255)
                                                                                                                                                                                                                                                     gtgGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAGACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGGCTTGAGTTAACGGTGCTgg                            <  1:582905/289‑1 (MQ=255)
                                                                                                                                                                                                                                                     gtgGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGGCTTGAGTTAACGGTGCTgg                            <  1:1038991/289‑1 (MQ=255)
                                                                                                                                                                                                                                                           gTAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATca                                                                                                                     <  2:797249/194‑1 (MQ=255)
                                                                                                                                                                                                                                                            tAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGctct                                                                               >  1:324541/1‑231 (MQ=255)
                                                                                                                                                                                                                                                            tAAAAGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGctct                                                                               <  2:324541/231‑1 (MQ=255)
                                                                                                                                                                                                                                                              aaaGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTa                                                          <  1:1840562/250‑1 (MQ=255)
                                                                                                                                                                                                                                                              aaaGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTa                                                          >  2:1840562/1‑250 (MQ=255)
                                                                                                                                                                                                                                                                aGGCATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCg                                                            >  1:901264/1‑246 (MQ=255)
                                                                                                                                                                                                                                                                   cATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGGCTTGAGTTAACGGTGCTGGATTCCCAGtgctg               <  1:463988/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                   cATTCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGGCTTGAGTTAACGGTGCTGGATTCCCAGtgctg               <  1:1324985/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                      tCCGGTGATTGCCACCTCCTCAACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAg                                                                    >  2:1708455/1‑232 (MQ=255)
                                                                                                                                                                                                                                                                                         |||                                                                                                                                                                                                                                                                                    
GGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAACTACAACCATCCGCAGTTAGGTAAAGATTTAATTAAAGTGCTCAACGCAATGGGTACCGGTGTACAACTGCTCAGCAAAGAAAAATGCTGCGGCGTACCGCTAATCGCCAACGGCTTTACCGATAAAGCACGCAAACAGGCAATTACGAATGTAGAGAGCATCCGCGAAGCTGTGGGAGTAAAAGGCATTCCGGTGATTGCCACCTCCAGTACCTGTACATTTGCCCTGCGCGACGAATACCCGGAAGTGCTGAATGTCGACAACAAAGGCTTGCGCGATCATATCGAACTGGCAACCCGCTGGCTGTGGCGCAAGCTGGACGAAGGCAAAACGTTACCGCTGAAACCGCTGCCGCTGAAAGTGGTTTATCACACTCCGTGCCATATGGAAAAAATGGGCTGGACGCTCTACACCCTGGAGCTGTTGCGTAACATCCCGGGGCTTGAGTTAACGGTGCTGGATTCCCAGTGCTGCGGTATTGCGGGT  >  CP042583/1927222‑1927781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A