Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F210 I1 R1 4928 385.2 6658600 97.5% 6492135 231.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 612,263 3 bp→TGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583612,2630AT99.7% 841.8 / NA 350intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (161/188);  minor base G (1/0);  total (162/188)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583612,2640CG99.4% 817.2 / ‑6.6 350intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (1/0);  major base G (160/186);  minor base T (1/1);  total (162/188)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
*CP042583612,2650TA98.9% 823.6 / NA 350intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/1);  major base A (161/182);  minor base G (1/3);  total (162/188)
Fisher's exact test for biased strand distribution p-value = 6.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 350 total aligned reads displayed.

GGAGAACGCCTTTTTCATTTCTCCCCAGATAGGCAATGGTCAGATAATACGCGGTAGGATTTTTCAGTGTCAGTTGGTTGCCCTGCTGGCTGACCTGTAACTGCAGTTCGACCTTTTCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTACTGGCTTGTTTCACAACCCGCACCTGGCTTGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCA  >  CP042583/611983‑612540
                                                                                                                                                                                                                                                                                          |||                                                                                                                                                                                                                                                                                   
       gCCTTTTTCATTTCTCCCCAGATAGGCAATGGTCAGATAATACGCGGTAGGATTTTTCAGTGTCAGTTGGTTGCCCTGCTGGCTGACCTGTAACTGCAGTTCGACCTTTTCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTcaca                                                                                                                                                                                                                                                                        <  1:604819/289‑1 (MQ=255)
         cTTTTTCATTTCTCCCCAGATAGGCAATGGTCAGATAATACGCGGTAGGATTTTTCAGTGTCAGTTGGTTGCCCTGCTGGCTGACCTGTAACTGCAGTTCGACCTTTTCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCGGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAATTTTCGCGCATATTTTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTTGT‑GTTGAGGCTGGTTTCACaa                                                                                                                                                                                                                                                                       >  2:2859489/1‑289 (MQ=255)
                    ctcCCCAGGTAGGCAATGGTCAGATAATACGCGGTAGTATTTTTCAGGGTCAGTGGGTTGCCCTGCTGGCTGACCTGTAACTGCAGTTCGAACTTTTCTCCTGCTGTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATTGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTgcg                                                                                                                                                                                                                                                           <  2:1297958/289‑3 (MQ=255)
                            aTAGGCAATGGTCAGATAATACGCGGTAGGATTTTTCAGTGTCAGTTGGTTGCCCTGCTGGCTGACCTGTAACTGCAGTTCGACCTTTTCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTG‑GTGTGTTGAGGCTTGTTTACCAACCCGGCCCTGCGATGTCGCt                                                                                                                                                                                                                                                   >  2:568760/1‑289 (MQ=255)
                               ggCAATGGTCAGATAATACGCGGTAGGATTTTTCAGTGTCAGTTGGTTGCCCTGCTGGCTGACCTGTAACTGCAGTTCGACCTTTTCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCt                                                                                                                                                                                                                                                   >  2:347460/1‑286 (MQ=255)
                                          gATAATACGCGGTAGGATTTTTCAGTGTCAGTTGGTTGCCCTGCTGGCTGACCTGTAACTGCAGTTCGACCTTTTCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACg                                                                                                                                                                                                                                     >  1:35565/1‑289 (MQ=255)
                                                aCGCGGTAGGATTTTTCAGTGTCAGTTGGTTGCCCTGCTGGCTGACCTGTAACTGCAGTTCGACCTTTTCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAAt                                                                                                                                                                                                                               <  1:2422977/289‑1 (MQ=255)
                                                 cgcgGTAGGATTTTTCAGTGTCAGTTGGTTGCCCTGCTGGCTGACCTGTAACTGCAGTTCGACCTTTTCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATa                                                                                                                                                                                                                              >  1:1761394/1‑289 (MQ=255)
                                                 cgcgGTAGGATTTTTCAGTGTCAGTTGGTTGCCCTGCTGGCTGACCTGTAACTGCAGTTCGACCTTTTCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATa                                                                                                                                                                                                                              <  2:2941399/289‑1 (MQ=255)
                                                 cgcgGTAGGATTTTTCAGTGTCAGTTGGTTGCCCTGCTGGCTGACCTGTAACTGCAGTTCGACCTTTTCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATa                                                                                                                                                                                                                              >  1:975355/1‑289 (MQ=255)
                                                     gTAGGATTTTTCAGTGTCAGTTGGTTGCCCTGCTGGCTGACCTGTAACTGCAGTTCGACCTTTTCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTcaca                                                                                                                                                                                                                                                                        <  1:77359/243‑1 (MQ=255)
                                                     gTAGGATTTTTCAGTGTCAGTTGGTTGCCCTGCTGGCTGACCTGTAACTGCAGTTCGACCTTTTCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAATCCGCACCTGCGATGTCGCTTTCGGTTTCAGATGTTGAATAgggg                                                                                                                                                                                                                          >  2:524171/1‑289 (MQ=255)
                                                     gTAGGATTTTTCAGTGTCAGTTGGTTGCCCTGCTGGCTGACCTGTAACTGCAGTTCGACCTTTTCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAgggg                                                                                                                                                                                                                          >  2:1707546/1‑289 (MQ=255)
                                                      tAGGATTTTTCAGTGTCAGTTGGTTGCCCTGCTGGCTGACCTGTAACTGCAGTTCGACCTTTTCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAACTAGCGTTTCGCGGTCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCATCTGCGCTCTCGCTTTCGGTTCCATAAGTTGAATAgtggg                                                                                                                                                                                                                         >  2:1905697/1‑289 (MQ=255)
                                                      tAGGATTTTTCAGTGTCAGTTGGTTGCCCTGCTGGCTGACCTGTAACTGCAGTTCGACCTTTTCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑CTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGTTGTGGCTTTCGGTTCCAGTCGTTGAATAggggg                                                                                                                                                                                                                         >  2:413452/1‑289 (MQ=255)
                                                         gATTTTTCAGTGTCAGTTGGTTGCCCTGCTGGCTGACCTGTAACTGCAGTTCGACCTTTTCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCttt                                                                                                                                                                                                                                                 <  2:2669494/262‑1 (MQ=255)
                                                         gATTTTTCAGTGTCAGTTGGTTGCCCTGCTGGCTGACCTGTAACTGCAGTTCGACCTTTTCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCaa                                                                                                                                                                                                                      >  2:476543/1‑289 (MQ=255)
                                                                     tCAGTTGGTTGCCCTGCTGGCTGACCTGTAACTGCAGTTCGACCTTTTCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTcc                                                                                                                                                                                                                                          <  2:2988801/257‑1 (MQ=255)
                                                                             ttgCCCTGCTGGCTGACCTGTAACTGCAGTTCGACCTTTTCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCg                                                                                                                                                                                                   <  1:524171/288‑1 (MQ=255)
                                                                               gCCCTGCTGGCTGACCTGTAACTGCAGTTCGACCTTTTCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTca                                                                                                                                                                                                                                                                          >  1:876350/1‑215 (MQ=255)
                                                                               gCCCTGCTGGCTGACCTGTAACTGCAGTTCGACCTTTTCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCg                                                                                                                                                                                                   >  1:2555124/1‑286 (MQ=255)
                                                                                  ctgctgGCTGACCTGTAACTGCAGTTCGACCTTTTCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTtc                                                                                                                                                                                             <  1:699870/289‑1 (MQ=255)
                                                                                       ggctgACCTGTAACTGCAGTTCGACCTTTTCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTcc                                                                                                                                                                                                                                          >  1:1355129/1‑239 (MQ=255)
                                                                                               tGTAACTGCAGTTCGACCTTTTCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCAt                                                                                                                                                                                >  1:1010654/1‑289 (MQ=255)
                                                                                                  aaCTGCAGTTCGACCTTTTCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCAtttt                                                                                                                                                                             <  2:2019258/289‑1 (MQ=255)
                                                                                                        aGTTCGACCTTTTCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCAtttt                                                                                                                                                                             >  1:397295/1‑283 (MQ=255)
                                                                                                        aGTTCGACCTTTTCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATc                                                                                                                                                                        <  2:2537103/288‑1 (MQ=255)
                                                                                                           tCGACCTTTTCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGGGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAg                                                                                                                                                                                                                                        <  1:1931094/221‑1 (MQ=255)
                                                                                                           tCGACCTTTTCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTccc                                                                                                                                                                                         <  2:1172525/268‑1 (MQ=255)
                                                                                                            cGACCTTTTCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGa                                                                                                                                                                   >  1:2620723/1‑289 (MQ=255)
                                                                                                                 ttttCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAg                                                                                                                                                              <  2:1474134/289‑1 (MQ=255)
                                                                                                                 ttttCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGAGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAg                                                                                                                                                              <  2:3217280/289‑1 (MQ=255)
                                                                                                                        cTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGTACCTGCGATGTCGCTTTCGGTTCCAGACGt                                                                                                                                                                                                                                    <  1:477063/212‑1 (MQ=255)
                                                                                                                          gCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCttt                                                                                                                                                                                                                                                 >  1:560149/1‑197 (MQ=255)
                                                                                                                           cTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAgg                                                                                                                                                    >  2:231726/1‑289 (MQ=255)
                                                                                                                               cTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGt                                                                                                                                                >  2:1511388/1‑289 (MQ=255)
                                                                                                                                    gTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGt                                                                                                                                            <  1:457209/288‑1 (MQ=255)
                                                                                                                                     tAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATcc                                                                                                                                                                       <  1:2247066/260‑1 (MQ=255)
                                                                                                                                       aTGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACt                                                                                                                                        <  2:573089/289‑1 (MQ=255)
                                                                                                                                        tGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTACTTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTTAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCCGGACATAGCAAGATCCGGCAGTTTGTCCCtt                                                                                                                                       >  2:3055693/1‑289 (MQ=255)
                                                                                                                                        tGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAAt                                                                                                                                                                 <  2:1836692/263‑1 (MQ=255)
                                                                                                                                                gTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCAt                                                                                                                                                                                                     <  2:1970401/219‑1 (MQ=255)
                                                                                                                                                gTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTc                                                                                                                               >  1:2046943/1‑289 (MQ=255)
                                                                                                                                                gTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCCCCCCTTTTTCATTTTCTACTCAGGAATAAGCAATATAAGGCAGTTTGTCACTTTGGCTTTc                                                                                                                               >  2:1790209/1‑289 (MQ=255)
                                                                                                                                                  cGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATcc                                                                                                                                                                       <  1:2974936/247‑1 (MQ=255)
                                                                                                                                                  cGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCCGGTTGTCCCTTTGGTTTTCgt                                                                                                                             >  2:1759085/1‑288 (MQ=255)
                                                                                                                                                         aCAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGCAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCa                                                                                                                       <  1:2186603/288‑1 (MQ=255)
                                                                                                                                                             tttAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAagag                                                                                                                                                                                                        <  2:1650557/203‑1 (MQ=255)
                                                                                                                                                                       cTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAgg                                                                                                                                                    <  2:2255250/245‑1 (MQ=255)
                                                                                                                                                                          tGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGAc                                                                                                                                                                                                                >  2:377593/1‑182 (MQ=255)
                                                                                                                                                                          tGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCAt                                                                                                     >  1:1492567/1‑289 (MQ=255)
                                                                                                                                                                          tGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCAt                                                                                                     <  1:1707546/289‑1 (MQ=255)
                                                                                                                                                                             aTAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGGAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTg                                                                                                   <  1:3053256/288‑1 (MQ=255)
                                                                                                                                                                                 cGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGAc                                                                                                                                                                                                                <  2:2727037/175‑1 (MQ=255)
                                                                                                                                                                                 cGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCAt                                                                                                     >  1:1631839/1‑282 (MQ=255)
                                                                                                                                                                                 cGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGtt                                                                                              >  1:2679088/1‑289 (MQ=255)
                                                                                                                                                                                  gACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGTCTGGTGGCTTTGTCTGTGGCGTTa                                                                                             >  2:1660622/1‑289 (MQ=255)
                                                                                                                                                                                  gACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTa                                                                                             <  1:231726/289‑1 (MQ=255)
                                                                                                                                                                                  gACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTa                                                                                             <  1:2335083/289‑1 (MQ=255)
                                                                                                                                                                                      tGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTa                                                                                             <  2:2095517/285‑1 (MQ=255)
                                                                                                                                                                                            aTCGCATTGTTGCTGCTTTTATTCGGGGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATACCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAAt                                                                                     <  2:270491/287‑1 (MQ=255)
                                                                                                                                                                                             tCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCttt                                                                                                                                                                                                                                                 <  2:2061644/130‑1 (MQ=255)
                                                                                                                                                                                              cGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCAt                                                                                                                                                                                                     <  1:2012255/173‑1 (MQ=255)
                                                                                                                                                                                              cGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCgt                                                                                 >  2:646019/1‑289 (MQ=255)
                                                                                                                                                                                                  tGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCCGGCTGGTGGCTTTGTCATTGGAGTTAAACACAATGTGTGTGc                                                                             >  2:2625894/1‑289 (MQ=255)
                                                                                                                                                                                                   ggTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATcc                                                                                                                                                                       >  1:146032/1‑198 (MQ=255)
                                                                                                                                                                                                     tCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAgggg                                                                                                                                                                                                                          <  2:2403255/145‑1 (MQ=255)
                                                                                                                                                                                                     tCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGAc                                                                                                                                                                                                                <  2:212571/155‑1 (MQ=255)
                                                                                                                                                                                                     tCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCAtttt                                                                                                                                                                             <  2:2484760/190‑1 (MQ=255)
                                                                                                                                                                                                     tCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCAtttt                                                                                                                                                                             >  1:2484760/1‑190 (MQ=255)
                                                                                                                                                                                                     tCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGa                                                                           <  1:2383347/288‑1 (MQ=255)
                                                                                                                                                                                                      cGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGAta                                                                         >  1:270491/1‑288 (MQ=255)
                                                                                                                                                                                                      cGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGAt                                                                          <  1:2662191/288‑1 (MQ=255)
                                                                                                                                                                                                       gctgctTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCg                                                                        >  2:2687038/1‑289 (MQ=255)
                                                                                                                                                                                                         tgctTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGtttt                                                                                                                                <  1:3110550/231‑1 (MQ=255)
                                                                                                                                                                                                         tgctTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGc                                                                                                                        >  2:1883636/1‑239 (MQ=255)
                                                                                                                                                                                                         tgctTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGc                                                                                                                        <  1:1883636/239‑1 (MQ=255)
                                                                                                                                                                                                               tATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCAtttt                                                                                                                                                                             <  2:882138/180‑1 (MQ=255)
                                                                                                                                                                                                               tATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCa                                                                 >  1:576588/1‑288 (MQ=255)
                                                                                                                                                                                                                aTCCGGAGCGGGAGGAATTTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCAc                                                                <  1:2859489/289‑1 (MQ=255)
                                                                                                                                                                                                                 tCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCAcc                                                               >  2:1124388/1‑288 (MQ=255)
                                                                                                                                                                                                                     gAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGa                                                                                                                                                                   >  1:3138640/1‑184 (MQ=255)
                                                                                                                                                                                                                         gggAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTg                                                      <  1:2103959/289‑1 (MQ=255)
                                                                                                                                                                                                                          ggaggaA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTg                                                      <  2:1016915/288‑1 (MQ=255)
                                                                                                                                                                                                                                a‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAAccc                                                <  1:1054578/288‑1 (MQ=255)
                                                                                                                                                                                                                                      tggattatTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACATGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTg                                          <  2:1435203/284‑1 (MQ=255)
                                                                                                                                                                                                                                      gcgcATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTg                                          >  1:2686082/1‑289 (MQ=255)
                                                                                                                                                                                                                                      gcgcATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTg                                          >  1:1827660/1‑289 (MQ=255)
                                                                                                                                                                                                                                               gtaagaaaaTAGCGTTTCGCGATCGTCAGGTAATTGTGTG‑GTTGTGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCTGTTCCAGACGTTGAATAGGGGGCAAAGCGAACAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTaac                                 <  2:3127926/284‑1 (MQ=255)
                                                                                                                                                                                                                                               gTAGAAAAATAGCGTTTCTCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTaac                                 <  2:760463/289‑1 (MQ=255)
                                                                                                                                                                                                                                               gTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTaac                                 <  2:843900/289‑1 (MQ=255)
                                                                                                                                                                                                                                                    aaaaTAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACAt                            >  1:657379/1‑289 (MQ=255)
                                                                                                                                                                                                                                                     aaaTAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACAtt                           <  1:2065200/289‑1 (MQ=255)
                                                                                                                                                                                                                                                        tAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGc                                                                                                                        <  1:121221/193‑1 (MQ=255)
                                                                                                                                                                                                                                                          gCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAgg                                                                                                                     >  2:2207065/1‑194 (MQ=255)
                                                                                                                                                                                                                                                              ttCGCGATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTaa                   <  1:3207095/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                 gcgATCGCCAGGTAATTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAATTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCt               <  2:2348863/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                         cAGGTAATTGGGTG‑GTTGCGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTaac                                 <  1:2915891/263‑1 (MQ=255)
                                                                                                                                                                                                                                                                              aaTTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCa  <  1:1662653/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                              aaTTGTGTG‑GTTGAGGCTTGTTTCACAACCCGCACCTGCGATGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCa  <  1:1532607/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                          |||                                                                                                                                                                                                                                                                                   
GGAGAACGCCTTTTTCATTTCTCCCCAGATAGGCAATGGTCAGATAATACGCGGTAGGATTTTTCAGTGTCAGTTGGTTGCCCTGCTGGCTGACCTGTAACTGCAGTTCGACCTTTTCTCCTGCTTTCTTGCGTAATGCAGCCGGTCGCCAGAACAATTTAATACGACTTTGAATAGCGACCTGAAGTATCGCATGGTCGCTGCTTTTATCCGGAGCGGGAGGAA‑TTTCGCGCATATTGTAGAAAAATAGCGTTTCGCGATCGCCAGGTAATTGTGTG‑GTACTGGCTTGTTTCACAACCCGCACCTGGCTTGTCGCTTTCGGTTCCAGACGTTGAATAGGGGGCAAAGCGACCAGAAGAGCATCGCTTTTCTCCCCTTTTTCATTTTCTATCCAGGAATAAGCAAGATAAGGCAGTTTGTCACTTTGGTTTTCGATACGCAGGCTGGTGGCTTTGTCATTGGCGTTAAACACAATGCGTGTGCGATCGGGTTGCACCGCAGCATTGCAACCCAAGCTGATGGTTAACAACATTAAGGGTAATCCTTTAATAAATGTCA  >  CP042583/611983‑612540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A