Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A4 F210 I1 R1 4930 262.8 3829204 96.6% 3699011 272.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,967,867 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,967,8670GC99.6% 526.8 / ‑3.7 226intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (121/104);  minor base . (1/0);  total (122/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01
*CP0425831,967,8680CG99.1% 521.1 / NA 226intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base G (120/104);  total (122/104)
Fisher's exact test for biased strand distribution p-value = 5.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01
*CP0425831,967,8690TA99.6% 526.2 / ‑4.8 226intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (120/104);  minor base G (1/0);  total (122/104)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01

Only 100 of 226 total aligned reads displayed.

CAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAAGCTCG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACTTCATACCGGAATATTCAATGTGGTAACCATCCGCCAGTTCCTGCTCGGCTTCCGGCTGGTCAAACGGGTGACGGTGACATACCGCCACGCCCGCGATGGCAAAGGTAATAAA  >  CP042583/1967589‑1968145
                                                                                                                                                                                                                                                                                      |||                                                                                                                                                                                                                                                                                     
cAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGaa                                                                                                                                                                                                                                                                              <  1:866130/289‑1 (MQ=255)
        tACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATg                                                                                                                                                                                                                                                                      >  2:1229328/1‑289 (MQ=255)
          cGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGaa                                                                                                                                                                                                                                                                    <  2:949490/289‑1 (MQ=255)
            cccACGTTAACGGGGGGGCGGATAGACCGGCTCTTCCGGGTACATTGGCGTTTGGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAAca                                                                                                                                                                                                                                                                  <  2:1822586/288‑1 (MQ=255)
             ccACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAAca                                                                                                                                                                                                                                                                  <  1:27487/288‑1 (MQ=255)
             ccACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAAca                                                                                                                                                                                                                                                                  <  1:186562/288‑1 (MQ=255)
                         ggcggcAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGTATCAAAATGAACATCATCATAAAGa                                                                                                                                                                                                                                                      <  2:231/288‑1 (MQ=255)
                           cggcAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAAca                                                                                                                                                                                                                                                                  >  2:371928/1‑274 (MQ=255)
                           cggcAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAAca                                                                                                                                                                                                                                                                  <  1:371928/274‑1 (MQ=255)
                                   aGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGACAGGCAGATTTTCCAGCCGAAGGACCTTACCTGGTCATAACGCGGACGCGGTAACGACC‑CACGAAGCAACATGAACATCATCATAATGAACGCGGGTTTc                                                                                                                                                                                                                                           >  1:1901401/1‑289 (MQ=255)
                                       cGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAgcg                                                                                                                                                                                                                                       <  2:1471555/289‑1 (MQ=255)
                                       cGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGAAAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAgcg                                                                                                                                                                                                                                       <  1:1134866/289‑1 (MQ=255)
                                        ggCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCCAAATCAAAATCATCATAAAGAACGCGGTTTTCAgcgc                                                                                                                                                                                                                                      >  2:149159/1‑289 (MQ=255)
                                          ctctTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGAGCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGTTAA‑CGCGCCACGAATCAAAATGAACATCATCAAAAAGAAAGCCGtaataaaccgcg                                                                                                                                                                                                                                    >  2:1802657/1‑278 (MQ=255)
                                               ccGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGTTTTCCCAGCCGAAGGACATCACCTGGTGCTACCGCGGACGCGGTAACCACG‑CAACAATCAAAGTAAACAACAGATAAAAGCACCAGGTTTTCAATGCCAACCaa                                                                                                                                                                                                                               >  2:728946/1‑288 (MQ=255)
                                                      cATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATg                                                                                                                                                                                                                        >  1:976251/1‑289 (MQ=255)
                                                       aTTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATg                                                                                                                                                                                                                        >  1:1729188/1‑288 (MQ=255)
                                                       aTTCGCGTTTCGCGTTTGGCGAACCGGTGCAGGCCGATCATCCAGTTACTCCGAACCTGGTTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATgg                                                                                                                                                                                                                       <  2:848199/289‑1 (MQ=255)
                                                         tcgcgtttcgcgttTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCg                                                                                                                                                                                                                     >  2:799334/1‑289 (MQ=255)
                                                           gcgtttcgcgtttGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGt                                                                                                                                                                                                                   <  1:712687/289‑1 (MQ=255)
                                                                tcgcgtttGGGAAAGCCGGGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTaacaa                                                                                                                                                                                                              <  2:1156307/289‑1 (MQ=255)
                                                                 cgcgtttGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTaacaac                                                                                                                                                                                                             >  1:632186/1‑289 (MQ=255)
                                                                     tttGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACggg                                                                                                                                                                                                          <  2:1729188/288‑1 (MQ=255)
                                                                              cgcgTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTgccagc                                                                                                                                                                                                <  1:757376/289‑1 (MQ=255)
                                                                                 gTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTgccagcca                                                                                                                                                                                              <  2:1165925/288‑1 (MQ=255)
                                                                                         cGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAAc                                                                                                                                                                                     <  1:411714/289‑1 (MQ=255)
                                                                                           atcatcCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTgccagc                                                                                                                                                                                                <  2:280658/276‑1 (MQ=255)
                                                                                           atcatcCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGAAG‑CACGAATCAAAAGGAACAATATCATCAATAACGCGGTTTTCAGCGCGAAAAAGATGAATGGCGGTAAACAAGGGCCTCTGCAGCGAACGAAGAACAg                                                                                                                                                                                   >  2:501660/1‑289 (MQ=255)
                                                                                           atcatcCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACA‑TACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGc                                                                                                                                                                                  >  1:1418024/1‑289 (MQ=255)
                                                                                            tcatcCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCCGCCACCGAAGAACAGc                                                                                                                                                                                  >  1:1160044/1‑289 (MQ=255)
                                                                                             catcCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGGCCGCCACCGAAGAACAGCg                                                                                                                                                                                 >  1:727792/1‑289 (MQ=255)
                                                                                               tcCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCa                                                                                                                                                                                                                                                                           >  2:1338710/1‑197 (MQ=255)
                                                                                               tcCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCa                                                                                                                                                                                                                                                                           <  1:1338710/197‑1 (MQ=255)
                                                                                               tcCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTgccagc                                                                                                                                                                                                <  2:67247/272‑1 (MQ=255)
                                                                                               tcCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTgccagc                                                                                                                                                                                                >  1:67247/1‑272 (MQ=255)
                                                                                                  aGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCCCCGAAAAACACGGTCAc                                                                                                                                                                             >  2:775304/1‑288 (MQ=255)
                                                                                                   gATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCa                                                                                                                                                                           >  1:152975/1‑289 (MQ=255)
                                                                                                    gaattccGAACCGGGGTGCCGAAACCGACGAACAATTCTTTTAAGGTCATGGTATTATTGCCCCTTATGGCGCCTGCCAAAGAATGACAGCCGCCGTTAACAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTCACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCAt                                                                                                                                                                          <  2:1581122/283‑1 (MQ=255)
                                                                                                     tactacGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCAt                                                                                                                                                                          <  1:63583/288‑1 (MQ=255)
                                                                                                     tactacGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCAt                                                                                                                                                                          <  1:402359/288‑1 (MQ=255)
                                                                                                     tactacGAACCTGGGTGCCGAAACCTACTAAAAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCAt                                                                                                                                                                          <  1:1020474/288‑1 (MQ=255)
                                                                                                     tacgacGAGCCTGGGTGCCGAAACCTACTAACAAGTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCAt                                                                                                                                                                          <  2:1133355/288‑1 (MQ=255)
                                                                                                          cGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAAca                                                                                                                                                                                                                                                                  <  2:1057076/195‑1 (MQ=255)
                                                                                                          cGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAAca                                                                                                                                                                                                                                                                  >  1:1057076/1‑195 (MQ=255)
                                                                                                          cGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGc                                                                                                                                                                    >  1:432978/1‑289 (MQ=255)
                                                                                                          cGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCCTCCACAACAATGc                                                                                                                                                                    >  2:1045128/1‑289 (MQ=255)
                                                                                                              ccTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCTTAACGCGGACGCGGTAACGACG‑CACGAATCAAAAGGAACATCATCATAAAGAACCCTGTTTTCAGCGCGAACCAGAGGAATGGCGGTAACAACGGGCCTGGCCAGCAACCTAAGAACAGCGTCCCCCGCAATgaacag                                                                                                                                                                >  2:1699655/1‑284 (MQ=255)
                                                                                                              ccTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCagag                                                                                                                                                                >  1:1296675/1‑289 (MQ=255)
                                                                                                                     gCCGCAACCTCCTAACAATTCTTTTAAGTTCTGGGTCTTATTGCCCCTTAGTGCGCCGGCCAGGGAATGACAGCCGCCGATACCAGCAAGTTGATCAGCGTCGGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCAGAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATAATCATAAAGAACGCGGTTTTCATCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTca                                                                                                                                                         <  2:408698/289‑1 (MQ=255)
                                                                                                                      ccGAAACCTACTAACAATTCTGTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTca                                                                                                                                                         <  2:632186/288‑1 (MQ=255)
                                                                                                                       cGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAGAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTcaca                                                                                                                                                       >  1:1026290/1‑289 (MQ=255)
                                                                                                                       cGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACTATCAATGCAGAGATGGTcaca                                                                                                                                                       <  1:149159/289‑1 (MQ=255)
                                                                                                                        tgaaactactaATAATTCTTTTCAGGTACTGGTCTTGTTGCCCCGTATTGCGGCTGCCAGGAAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACaa                                                                                                                                                      <  2:1777358/284‑1 (MQ=255)
                                                                                                                         aaaCCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTgccagc                                                                                                                                                                                                >  1:156093/1‑246 (MQ=255)
                                                                                                                                       ttCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCAACAATGCAGAAGTGGTCAACATCCCGATGTACCCa                                                                                                                                        >  2:898681/1‑288 (MQ=255)
                                                                                                                                                    aTGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCCTCACCATCAATGCAGAGATCGTCACAATCCCGAAGGAACCACCCACCAAGAAcca                                                                                                                          >  2:1140949/1‑287 (MQ=255)
                                                                                                                                                     tGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGa                                                                                                                         <  1:785801/289‑1 (MQ=255)
                                                                                                                                                            aTTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACt                                                                                                                  >  1:503989/1‑289 (MQ=255)
                                                                                                                                                                  ccTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGCCGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGAGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGCACAGCGTCACCATCAAGGCAGAGATGGTCACAACCCCGAAGTACTCACCCACGAAGCACAAACCGAACTTCATa                                                                                                             >  2:1093826/1‑288 (MQ=255)
                                                                                                                                                                  ccTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTgccagc                                                                                                                                                                                                <  2:1902048/205‑1 (MQ=255)
                                                                                                                                                                  ccTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGt                                                                                                                                                           <  1:1281106/242‑1 (MQ=255)
                                                                                                                                                                  ccTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGt                                                                                                                                                           >  2:1281106/1‑242 (MQ=255)
                                                                                                                                                                  ccTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACTTCATa                                                                                                             >  1:853685/1‑288 (MQ=255)
                                                                                                                                                                  ccTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGGACTCACCCACGAAAAACAAACCGAACCTCATa                                                                                                             >  2:696181/1‑288 (MQ=255)
                                                                                                                                                                  ccTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACAACATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCATTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACCCAACCACCAAGAAAAGACCGAACGTCATa                                                                                                             >  2:334690/1‑288 (MQ=255)
                                                                                                                                                                    ttATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACTTCATACCg                                                                                                          <  1:472508/289‑1 (MQ=255)
                                                                                                                                                                      aTTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACTTCATACCGGa                                                                                                        <  2:515042/289‑1 (MQ=255)
                                                                                                                                                                          cgcCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGa                                                                                                                                                                                                                                                      >  1:196120/1‑143 (MQ=255)
                                                                                                                                                                          cgcCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGa                                                                                                                                                                                                                                                      <  2:196120/143‑1 (MQ=255)
                                                                                                                                                                           gcCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACTTCATACCGGAATAtt                                                                                                   <  1:1480076/289‑1 (MQ=255)
                                                                                                                                                                            ccTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGGTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACTTCATACCGGAATATTc                                                                                                  >  1:898944/1‑289 (MQ=255)
                                                                                                                                                                            ccTGCCAGAGAAGGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACTTCATACCGGAATATTc                                                                                                  <  1:1774334/289‑1 (MQ=255)
                                                                                                                                                                             cTGCCAGAGAATGACAGCCGTCGTTACCAGCAAATTGATCAGCGGCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑AACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACTGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACTTCATACCGGAATATTCa                                                                                                 <  2:973163/289‑1 (MQ=255)
                                                                                                                                                                             cTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAGAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACTTCATACCGGAATATTCa                                                                                                 <  2:1026290/289‑1 (MQ=255)
                                                                                                                                                                             cTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACTTCATACCGGAATATTCa                                                                                                 <  2:1623583/289‑1 (MQ=255)
                                                                                                                                                                               gCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCgg                                                                                                                                                                                                                    <  1:834938/172‑1 (MQ=255)
                                                                                                                                                                               gCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCgg                                                                                                                                                                                                                    >  2:834938/1‑172 (MQ=255)
                                                                                                                                                                               gCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACTTCATACCGGAATATTCAAt                                                                                               >  1:1615381/1‑289 (MQ=255)
                                                                                                                                                                               gCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACTTCATACCGGAATATTCAAt                                                                                               >  1:326637/1‑289 (MQ=255)
                                                                                                                                                                               gCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGAACGAACTTCATACCGGAATATTCGAt                                                                                               >  1:552815/1‑289 (MQ=255)
                                                                                                                                                                                              ccgccgTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACtt                                                                                                                 <  2:928206/256‑1 (MQ=255)
                                                                                                                                                                                              ccgccgTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACtt                                                                                                                 >  1:928206/1‑256 (MQ=255)
                                                                                                                                                                                                           cAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACTTCATACCGGAATATTCAATGTGGTAACCATCCGCCAGTTCCTGCTCg                                                                   >  2:1545172/1‑289 (MQ=255)
                                                                                                                                                                                                           cAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACTTCATACCGGAATATTCAATGTGGTAACCATCCGCCAGTTCCTGCTCg                                                                   >  1:1672922/1‑289 (MQ=255)
                                                                                                                                                                                                                       cgtcagcgGCAGGCAGTTTTTCCAGCCGCAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CTCGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACTTCATACCGGAATATTCAACGTGGTAACCATCCGCCAGTTCCTGCCCGCCTTCCGGCTgg                                                       >  2:636157/1‑289 (MQ=255)
                                                                                                                                                                                                                       cgtcagcgGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACTTCATACCGGAATATTCAATGTGGTAACCATCCGCCAGTTCCTGCTCGGCTTCCGGCTgg                                                       >  2:185066/1‑289 (MQ=255)
                                                                                                                                                                                                                       cgtcagcgGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCACGATGTACCCACCCACGAAGAACAGACCGAACTTCATACCGGACAATTCAATGTGGTAACCATCCGCCAGGTCATGCCCGGCTTCCGGCTgg                                                       >  2:658545/1‑289 (MQ=255)
                                                                                                                                                                                                                            gcgGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACTTCATACCGGAATATTCAATGTGGTAACCATCCGCCAGTTCCTGCTCGGCTTCCGGCTGGTCaaa                                                  >  2:509565/1‑289 (MQ=255)
                                                                                                                                                                                                                             cgGCAGGCAGAATTTCCAGCCGAAGGACATTACCTGGTCATAACGCCGACGCGGTACCGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTCACACCGGGCCTTCCCCGCCACCGAAGTACAGCATCACCATCAATCCAGAGATGGGCACAATCCCGTAGTACACACCCAACAAGAACACAACGAAGTTCACACCGGCATACTCATAGAGATCACCACCCGCCAGGTCCTGCTCGCCTCCCGGCttgcaaac                                                  >  2:1286174/1‑281 (MQ=255)
                                                                                                                                                                                                                              ggcaggcaGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCATGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACTTCATACCGGAATATTCAATGTGGTAACCATCCGCCAGTTCCTGCTCGGCTTCCGGCTGGTCaa                                                   <  1:1229328/286‑1 (MQ=255)
                                                                                                                                                                                                                                 aggcagATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACTTCATACCGGAATATTCAATGTGGTAACCATCCGCCAGTTCCTGCt                                                                     <  1:454777/265‑1 (MQ=255)
                                                                                                                                                                                                                                 aggcagATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACTTCATACCGGAATATTCAATGTGGTAACCATCCGCCAGTTCCTGCt                                                                     >  2:454777/1‑265 (MQ=255)
                                                                                                                                                                                                                                    cagATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACTTCATACCGGAATATTCAATGTGGTAACCATCCGCCAGTTCCTGCTCGGCTTCCGGCTGGTCAAACGggtga                                           <  1:1132966/288‑1 (MQ=255)
                                                                                                                                                                                                                                            ccAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACTTCATACCGGAATATTCAATGTGGTAACCATCCGCCAGTTCCTGCTCGGCTTCCGGCTGGTCAAACGGGTGACGGTGACAt                                  <  1:1093826/289‑1 (MQ=255)
                                                                                                                                                                                                                                                    aGGACATTACCTGGTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACTTCATACCGGAATATTCAATGTGGTAACCATCCGCCAGTTCCTGCTCGGCTTCCGGCTGGTCAAACGGGTGACGGTGACATACCGCCAc                          <  2:755484/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                ggTCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCACGATGTACTCACCCACGAAGAACAGACCGAACTTCATACCGGAATATTCAATGTGGTAACCATCCGCCAGTTCCTGCTCGGCTTCCGGCTGGTCAAACGGGTGACGGGGACATACCGCCACGCCCGCGATgg               >  1:1196851/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                  tCATAACGCGGACGCGGTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACTTCATACCGGAATATTCAATGTGGTAACCATCCGCCAGTTCCTGCTCGGCTTCCGGCTGGTCAAACGGGTGACGGTGACATACCGCCACGCCCGCGATGGCa             <  1:1178794/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                       acgcggacgcggTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTc                                                                                                                                                                               >  1:294805/1‑121 (MQ=255)
                                                                                                                                                                                                                                                                            gacgcggTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACTTCATACCGGAATATTCAATGTGGTAACCATCCGCCAGTTCCTGCTCGGCTTCCGGCTGGTCAAACGGGTGACGGTGACATACCGCCACGCCCGCGATGGCAAAGGTAATaaa  >  2:546358/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                            gacgcggTAACGACG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACTTCATACCGGAATATTCAATGTGGTAACCATCCGCCAGTTCCTGCTCGGCTTCCGGCTGGTCAAACGGGTGACGGTGACATACCGCCACGCCCGCGATGGCAAAGGTAATaaa  >  1:75184/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                      |||                                                                                                                                                                                                                                                                                     
CAGAACGATACGACCACGATAACGGGGCGGCAGATAGACCGGCTCTTCCGGGTACATTCGCGTTTCGCGTTTGGCGAACGCGTGCAGGCCGATCATCCAGATACTACGAACCTGGGTGCCGAAACCTACTAACAATTCTTTTAAGGTCATGGTCTTATTGCCCCTTATTGCGCCTGCCAGAGAATGACAGCCGCCGTTACCAGCAAGTTGATCAGCGTCAGCGGCAGGCAGATTTTCCAGCCGAAGGACATTACCTGGTCATAACGCGGACGCGGTAAGCTCG‑CACGAATCAAAATGAACATCATCATAAAGAACGCGGTTTTCAGCGCGAACCAGATGAATGGCGGTAACAACGGGCCTTGCCAGCCACCGAAGAACAGCGTCACCATCAATGCAGAGATGGTCACAATCCCGATGTACTCACCCACGAAGAACAGACCGAACTTCATACCGGAATATTCAATGTGGTAACCATCCGCCAGTTCCTGCTCGGCTTCCGGCTGGTCAAACGGGTGACGGTGACATACCGCCACGCCCGCGATGGCAAAGGTAATAAA  >  CP042583/1967589‑1968145

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A