Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F210 I1 R1 4929 268.4 3907572 96.3% 3762991 273.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,923,397 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,923,3970GC99.6% 622.7 / NA 264intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (146/117);  minor base T (1/0);  total (147/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,923,3980CG99.2% 623.2 / NA 264intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base G (145/117);  total (147/117)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,923,3990TA99.6% 629.6 / NA 264intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (146/117);  minor base C (1/0);  total (147/117)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 264 total aligned reads displayed.

ATACCACCACCGACGTTGTGCGCACAGTTCCACACACTCACAATGCCGCCACGTTCTTTCTGGCTCCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTGCTCGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTGCTGCGATCGCTTACGCTACCCATGATGAATTTGCTAAATCCATAAGCAATGCTGAT‑CCCGCTAAGGGCAAAACCTAAATCACCGCGACTGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCTTATTTATGATTAACAGC  >  CP042583/1923122‑1923675
                                                                                                                                                                                                                                                                                   |||                                                                                                                                                                                                                                                                                     
atcccacccccGACGTTGTGCGAACAGTTCCCAACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTCCGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAg                                                                                                                                                                                                                                                                            <  2:1182251/286‑1 (MQ=255)
         ccGACGTGCAGCGCACAATTCCACACGGACACAAGGCCGCCCCGTTCTTTCTGCGACCACCAGTGCACCATAGGACGACCACACGGCGGCCACCCCAACCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAAtcgaga                                                                                                                                                                                                                                                                   <  2:1361161/289‑6 (MQ=255)
            aCGTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAAtcgagatc                                                                                                                                                                                                                                                                 >  2:369756/1‑281 (MQ=255)
              gTTGTGCGCACAGTTCCACACTGACACAATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGTCCATGGCACAAAGCCCATAAACAACATCACTGCCACCGCCAGCATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCCATCCGATACCGAACCCATGATGAATTTCGAAAAACCATAAGCAATCAAGAT‑ccc                                                                                                                                                                                                                                                              >  1:1440200/1‑288 (MQ=255)
                              cacactgacacaATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAAAT‑CCACCAAAGGGCAAAACCTa                                                                                                                                                                                                                                             >  2:403134/9‑289 (MQ=255)
                                     acacaATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACACAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAAGT‑CCCCGAAAGGGCAAAACCAAAATCAc                                                                                                                                                                                                                                       >  2:352900/2‑288 (MQ=255)
                                     acacaATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGCATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCAc                                                                                                                                                                                                                                       >  2:1226861/2‑288 (MQ=255)
                                     acacaATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCCGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCCAAAATCCATAAGCAAACGAAAA‑CCCCGAAAGGGCAAAACCAAAATCAc                                                                                                                                                                                                                                       >  2:1766724/2‑288 (MQ=255)
                                     acacaATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCAcc                                                                                                                                                                                                                                      >  1:1388661/2‑289 (MQ=255)
                                     acacaATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCAc                                                                                                                                                                                                                                       >  1:829171/2‑288 (MQ=255)
                                     acacaATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAC‑CCCCGAAAGGGCAAAACCTAAATCAc                                                                                                                                                                                                                                       >  1:1345055/2‑288 (MQ=255)
                                     acacaATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGACTCGAGCGATCCGATACCGAACACATGATGAACTTCGAAAATCCATAAGCAATCACGATACCCGC‑ACAGGCAAAACCTaaacaac                                                                                                                                                                                                                                       >  2:1682393/2‑284 (MQ=255)
                                     acacaATGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCCGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCAc                                                                                                                                                                                                                                       >  2:1134754/2‑288 (MQ=255)
                                          aTGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAAGCCATAAGCAATCGAGAT‑ACCCGAAAGGGCAAAACCTAAATCACcgagtg                                                                                                                                                                                                                                 >  1:1225119/1‑285 (MQ=255)
                                           tGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACcgcgtga                                                                                                                                                                                                                                >  1:53283/1‑286 (MQ=255)
                                           tGCCGCCACGTTCTTTCTGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGAAACCGAACCCATGATGAATTTCCAAAATCCATAAGCAATGGAGAG‑CCCCAAAAAGGCAAAAACTAAATAAcctccgaa                                                                                                                                                                                                                                >  2:1038004/1‑283 (MQ=255)
                                                      tctttctGCGACCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCt                                                                                                                                                                                                                     <  1:568109/289‑1 (MQ=255)
                                                        tttctgcgaccaccaGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCa                                                                                                                                                                                                                                                    >  2:1576067/10‑256 (MQ=255)
                                                         ttctgcgagcaccaGTGAACCGAAGTACGACCACACGGCGGACCACCCATCCCCTGGAACCAACCACAGAGGAAAAACAGTAAAAAAAACACCGCAATGCGCGACGTCGGCCATGGAACAAAGCCCATAAAAAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCaa                                                                                                                                                                                                                  <  2:1914709/280‑1 (MQ=255)
                                                         ttctgcgaccaccaGTGCACCATATTACGACCACCCGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGCCGTCGCCCATGGCACAAAGCCCATAAACAACATCCCTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCaa                                                                                                                                                                                                                  <  1:1682393/281‑1 (MQ=255)
                                                         ttctgcgaccaccaGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCaa                                                                                                                                                                                                                  <  1:171082/281‑1 (MQ=255)
                                                         ttctgagaccaccgGTGCACCATAGAACGACCCCACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCaa                                                                                                                                                                                                                  <  2:829171/281‑1 (MQ=255)
                                                          tctgcgaccaccaGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTg                                                                                                                                                                                                                       <  1:72568/276‑1 (MQ=255)
                                                                    ccaGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCCCAACCAGATAAGGc                                                                                                                                                                                                       >  2:624019/1‑289 (MQ=255)
                                                                          cACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGc                                                                                                                                                                                                  <  1:434735/288‑1 (MQ=255)
                                                                               aaGTACGACCACACCGCCGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAgcgc                                                                                                                                                                                            <  2:53283/288‑1 (MQ=255)
                                                                                aGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCa                                                                                                                                                                                           <  1:1622014/289‑1 (MQ=255)
                                                                                  tACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCTCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGTCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCTCaaa                                                                                                                                                                                         >  1:1691943/1‑289 (MQ=255)
                                                                                       ccACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCt                                                                                                                                                                                                                     <  1:1298483/256‑1 (MQ=255)
                                                                                       ccACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCt                                                                                                                                                                                                                     >  2:1298483/1‑256 (MQ=255)
                                                                                       ccACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCt                                                                                                                                                                                    >  1:1920582/1‑289 (MQ=255)
                                                                                          cacGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTAc                                                                                                                                                                                 >  1:657143/1‑289 (MQ=255)
                                                                                          cacGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGCTCTAAc                                                                                                                                                                                 >  1:628464/1‑289 (MQ=255)
                                                                                           acGGCGGCCCCCCCACCCCCTGGAGAAAACCGAAAAGGAAAAACAGTAAAAACAACACCGCAAAGCGCGACGTCGCCCATGGCACAAAGACCATAAAAAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACg                                                                                                                                                                                <  2:1388661/289‑1 (MQ=255)
                                                                                             ggcggcCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGaa                                                                                                                                                                              <  1:1317825/289‑1 (MQ=255)
                                                                                               cggcCACCCCAGCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGATCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGCCAGGAAAACGCGCGGATTCCAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGAGAGAATCCCTGATCAACCCGATAAGGCATAGCAAGCGCAAAAGTCTTACAAAcc                                                                                                                                                                            >  2:51098/1‑289 (MQ=255)
                                                                                                 gcCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCaa                                                                                                                                                                          >  1:1561476/1‑289 (MQ=255)
                                                                                                    aCCCCCTCCCCTGAAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATa                                                                                                                                                                       <  1:608490/289‑1 (MQ=255)
                                                                                                     ccccATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAg                                                                                                                                                                      >  1:316474/1‑289 (MQ=255)
                                                                                                     ccccATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAg                                                                                                                                                                      >  1:594974/1‑289 (MQ=255)
                                                                                                              cggaaacacaccagagagGAAAAACAGAACAAACACCACCACAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCa                                                                                                                                                             <  2:1210808/275‑1 (MQ=255)
                                                                                                              ccggAACAAACCGCAGGGGACAAAAGGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGAACAAAGCCCATAAACACAAGCACTGCCGCCGCCAGAATAAAACCTGCGGGCAGGAAAACGGGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCa                                                                                                                                                             <  2:57368/287‑1 (MQ=255)
                                                                                                              cTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCa                                                                                                                                                             <  1:325208/289‑1 (MQ=255)
                                                                                                              cTGGAACCAACCGCAGAAGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCa                                                                                                                                                             <  1:1038004/289‑1 (MQ=255)
                                                                                                               tGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCaa                                                                                                                                                                                                                  <  1:51749/235‑1 (MQ=255)
                                                                                                                ggAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATa                                                                                                                                                           >  1:334970/1‑289 (MQ=255)
                                                                                                                ggAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCCCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCCAATAGTAAGCCGCATa                                                                                                                                                           >  1:154207/1‑289 (MQ=255)
                                                                                                                  aaccaaccGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAg                                                                                                                                                          <  1:1797959/288‑1 (MQ=255)
                                                                                                                   acacaccGCAGAAGAAAAACAGTACAACAATAACGCGAATGCACGACGGCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGcc                                                                                                                                                        <  2:1614648/285‑1 (MQ=255)
                                                                                                                    ccaaccGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCa                                                                                                                                                       <  1:368626/289‑1 (MQ=255)
                                                                                                                     caaccGCAGAGGAACAACAGTACAAACATCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCaa                                                                                                                                                      >  1:261877/1‑289 (MQ=255)
                                                                                                                            aggggAACAAAAGTACAACCAAGACCGACAGGCTCGACGTCCACCATGGCACAAAGCCCATAAACCACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAatat                                                                                                                                               <  2:1726722/286‑1 (MQ=255)
                                                                                                                                                 tCACCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAgcg                                                                                                                          <  1:1214372/289‑1 (MQ=255)
                                                                                                                                                   aCCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCa                                                                                                                        >  2:319631/1‑289 (MQ=255)
                                                                                                                                                   aCCGCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCa                                                                                                                        >  2:1174375/1‑289 (MQ=255)
                                                                                                                                                      gCAATGCTCGACGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATc                                                                                                                     <  1:1592012/289‑1 (MQ=255)
                                                                                                                                                            ctcgacgtcgCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACcgcgtg                                                                                                                                                                                                                                 <  2:1877312/170‑3 (MQ=255)
                                                                                                                                                            cgggagctagCCCCTGGCAACAAACCCGTAAATAGAATCACGGACGCCGCCCGAATCTAAACTGCGGCCCGGAAAACTCGCGGATGCGAGTGGTGCGATACCGAACCCACGATGAATTTCGAAGATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATcgacg                                                                                                                 <  2:1554763/278‑1 (MQ=255)
                                                                                                                                                             tcgacgtcgCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGAt                                                                                                               <  2:388620/284‑1 (MQ=255)
                                                                                                                                                             tcgacggcgCCCATGGGACAAAGCCCATAAACAACATCACTGCCGGCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGAGTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGAt                                                                                                               <  2:742526/281‑1 (MQ=255)
                                                                                                                                                                    cgCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGGCggc                                                                                                       >  1:879539/1‑288 (MQ=255)
                                                                                                                                                                      cccATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATAACCCCAGGAAAATTTGCCAGCGCAAACTACGATAAATCGgatc                                                                                                     >  2:687102/1‑289 (MQ=255)
                                                                                                                                                                              caaaaGCCCATAAACAACATCCCTGCCGCCGCCAGAATCAAACCGGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCcg                                                                                             <  2:594974/287‑1 (MQ=255)
                                                                                                                                                                                 aaGCCCATAAACAACATCACAGGCGGCGCCAGAAGCAAACCTGCGGGCAGGAAAACGCGCGGATTAGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCgg                                                                                          <  2:1454557/289‑1 (MQ=255)
                                                                                                                                                                                     ccATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAgg                                                                                      <  2:261877/289‑1 (MQ=255)
                                                                                                                                                                                                aTCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCtt                                                                           >  1:482032/1‑289 (MQ=255)
                                                                                                                                                                                                    cTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGACCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGCATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCCATCTCCGCGGAAGGTAcgcgcgacgtgcg                                                                       >  2:1362152/1‑282 (MQ=255)
                                                                                                                                                                                                     tGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTa                                                                                                                                                                    <  1:1479816/195‑1 (MQ=255)
                                                                                                                                                                                                     tGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTtgtgt                                                                      >  1:250241/1‑289 (MQ=255)
                                                                                                                                                                                                           cgccAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGgcgc                                                                 <  1:385212/288‑1 (MQ=255)
                                                                                                                                                                                                           cgccAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAAGCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGgcgc                                                                 <  2:809408/288‑1 (MQ=255)
                                                                                                                                                                                                                         cTGCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACt                                                  <  1:1063316/289‑1 (MQ=255)
                                                                                                                                                                                                                         cTGCGGGCAGGAAAACGCGCGGAATCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACt                                                  <  2:1497682/289‑1 (MQ=255)
                                                                                                                                                                                                                           gCGGGCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATACGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCACACGACGATACGTCGGCACGATCTCCGCGGCAGGTAAGCCCCGTTTGTGGGGCGCTTGTGTAACAATACTCa                                                >  2:1092574/1‑289 (MQ=255)
                                                                                                                                                                                                                             gggCAGGAAAACGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCaa                                               <  2:250241/288‑1 (MQ=255)
                                                                                                                                                                                                                                     aaaCGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAg                                      <  1:1766724/289‑1 (MQ=255)
                                                                                                                                                                                                                                      aaCGCGCGGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGc                                     <  1:977755/289‑1 (MQ=255)
                                                                                                                                                                                                                                        cgcgcgGATTCGAGCGATCCGATACCGAACCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGcc                                    >  1:178813/1‑288 (MQ=255)
                                                                                                                                                                                                                                                         tccgataccgaaCCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGc                                     <  1:1368986/259‑1 (MQ=255)
                                                                                                                                                                                                                                                         tccgataccgaaCCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGc                                     >  2:1368986/12‑270 (MQ=255)
                                                                                                                                                                                                                                                           cgataccgaaCCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCttat                <  2:178076/280‑1 (MQ=255)
                                                                                                                                                                                                                                                                   aaCCCATGATGAATTTCGCAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCGTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCTTATTTATGAtt        >  1:1949944/2‑289 (MQ=255)
                                                                                                                                                                                                                                                                   aaCCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAg                                                                                                            <  2:220381/188‑1 (MQ=255)
                                                                                                                                                                                                                                                                   aaCCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAg                                                                                                            >  1:220381/2‑189 (MQ=255)
                                                                                                                                                                                                                                                                   aaCCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGgcg                                                                  <  2:1070890/230‑1 (MQ=255)
                                                                                                                                                                                                                                                                   aaCCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACa                                             >  1:1749035/2‑252 (MQ=255)
                                                                                                                                                                                                                                                                   aaCCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTc                    <  1:797812/276‑1 (MQ=255)
                                                                                                                                                                                                                                                                   aaCCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCTTATTTATGact        >  1:1498163/2‑287 (MQ=255)
                                                                                                                                                                                                                                                                   aaCCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCTTATTTATGAtt        >  2:170223/2‑289 (MQ=255)
                                                                                                                                                                                                                                                                   aaCCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCTTATTTATGAtt        >  1:669559/2‑289 (MQ=255)
                                                                                                                                                                                                                                                                   aaCCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCTTATTTATGAtt        >  2:589062/2‑289 (MQ=255)
                                                                                                                                                                                                                                                                   aaCCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCTTATTTATGAtt        >  1:1229872/2‑289 (MQ=255)
                                                                                                                                                                                                                                                                   aaCCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCTTATTTATGAtt        >  2:718752/2‑289 (MQ=255)
                                                                                                                                                                                                                                                                   aaCCCATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGACGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCTTATTTATGAtt        >  1:1157107/2‑289 (MQ=255)
                                                                                                                                                                                                                                                                     cccATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCTTATTTATGATTaa      <  1:1174375/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                       cATGATGAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCTTATTTATGATTAACa    <  1:1104246/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                        atgatgAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCg                         <  1:445950/267‑1 (MQ=255)
                                                                                                                                                                                                                                                                        atgatgAATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTTGTCTTATTTATGATTAACa    >  2:935633/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                          gatgaATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAg                                      >  2:378893/1‑252 (MQ=255)
                                                                                                                                                                                                                                                                          gatgaATTTCGAAAATCCATAAGCAATCGAGAT‑CCCCGAAAGGGCAAAACCTAAATCACCGCGTGAGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAg                                      <  1:378893/252‑1 (MQ=255)
                                                                                                                                                                                                                                                                                   |||                                                                                                                                                                                                                                                                                     
ATACCACCACCGACGTTGTGCGCACAGTTCCACACACTCACAATGCCGCCACGTTCTTTCTGGCTCCACCAGTGCACCATAGTACGACCACACGGCGGCCACCCCATCCCCTGGAACCAACCGCAGAGGAACAACAGTACAAACATCACCGCAATGCTGCTCGTCGCCCATGGCACAAAGCCCATAAACAACATCACTGCCGCCGCCAGAATCAAACCTGCGGGCAGGAAAACGCGCGGATTGCTGCGATCGCTTACGCTACCCATGATGAATTTGCTAAATCCATAAGCAATGCTGAT‑CCCGCTAAGGGCAAAACCTAAATCACCGCGACTGAATCCCTGCTCAACCAGATAAGGCATAGCAAGCGCAAAGTTCTTACGAACCAAATAGTAAGCCGCATAGCCAAAGAATATCCCCAGGAAAATTTGCCAGCGCAATCGACGATAAGTCGGATCGATCTCCGCGGCAGGTAAGCGCGCTTTGTGTGGCGCTGGTTTAAAAATACTCAACATTGATAGCCTCCGTGGCCCGTGGTCTTATTTATGATTAACAGC  >  CP042583/1923122‑1923675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A