Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F210 I1 R1 4929 268.4 3907572 96.3% 3762991 273.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,952,920 3 bp→TCA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,952,9200AT99.7% 749.1 / NA 313intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (170/142);  minor base G (0/1);  total (170/143)
Fisher's exact test for biased strand distribution p-value = 4.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,952,9210GC99.0% 723.6 / NA 313intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base C (168/139);  minor base A (0/3);  total (170/143)
Fisher's exact test for biased strand distribution p-value = 9.50e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,952,9220TA99.4% 735.4 / NA 313intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (169/140);  minor base G (0/2);  total (170/143)
Fisher's exact test for biased strand distribution p-value = 2.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 313 total aligned reads displayed.

TCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGATAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACGCTTAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTAGTGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATGAGTGGCATTATCCAACCCTTAACGATTTATGGTCCGCAAGGTATCCGTGAA  >  CP042583/1952642‑1953192
                                                                                                                                                                                                                                                                                       |||                                                                                                                                                                                                                                                                               
tCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGATAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCCCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGgtgt                                                                                                                                                                                                                                                                         >  2:18382/1‑289 (MQ=255)
        gCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGATAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAAccc                                                                                                                                                                                                                                                                 >  1:1356022/1‑289 (MQ=255)
                    tcatTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGATAAGGGCAATTCTGCTCAACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATg                                                                                                                                                                                                                                                     >  1:329643/1‑289 (MQ=255)
                          ttCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGATAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTTATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACg                                                                                                                                                                                                                                                >  1:1408113/1‑288 (MQ=255)
                          ttCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGATAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACg                                                                                                                                                                                                                                                >  1:481463/1‑288 (MQ=255)
                               aCCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGATAAGGGCAATTCTGTTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTTATGAACTCATGGAATTAATTTTTTTAGGTAATTCAGCCGGTTTGCCAACCAGCACGCTCAATGTccacgcagca                                                                                                                                                                                                                                          >  2:1519107/1‑280 (MQ=255)
                                      atgaggCAATTATCCCCCGTCAAGTCATCACCATGGATATCCTGATAAGGGCAATTTCGATCGACAACGAAAACCGCGCAACGCAGTTGTAATATAGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTGCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTGTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTTCTTAAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCt                                                                                                                                                                                                                                     <  2:1389332/283‑1 (MQ=255)
                                              aTTATCCCCCGTCAAATCATCACCATCGATATCCTGATAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCa                                                                                                                                                                                                                            <  2:1915461/288‑1 (MQ=255)
                                                 aTCCCCCATCAAATCAACACCCACGATATCCTGATAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACAt                                                                                                                                                                                                                        <  2:281715/289‑1 (MQ=255)
                                                         tCAAATCATCACCATCGATATCCTGATAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCa                                                                                                                                                                                                                                                              <  2:137028/243‑1 (MQ=255)
                                                         tCAAATCATCACCATCGATATCCTGATAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCa                                                                                                                                                                                                                                                              >  1:137028/1‑243 (MQ=255)
                                                              tcatcaCCATCGAAATCCTGATAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCg                                                                                                                                                                                                           <  1:1004091/289‑1 (MQ=255)
                                                                   aCCATCGATATCCTGATAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACtt                                                                                                                                                                                                      >  2:1229635/1‑289 (MQ=255)
                                                                    ccATCGATATCCTGATAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACttt                                                                                                                                                                                                     <  1:1520717/289‑1 (MQ=255)
                                                                    ccATCGATACCCTGATAAGGGCAATTCCGCTAGACAACCAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACttt                                                                                                                                                                                                     <  2:1264739/289‑1 (MQ=255)
                                                                       tCGATATCCTGATAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGgt                                                                                                                                                                                                  >  1:1427470/1‑289 (MQ=255)
                                                                              ccTGATAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGa                                                                                                                                                                                           <  2:25734/289‑1 (MQ=255)
                                                                                 gATAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACt                                                                                                                                                                                         >  2:1129900/1‑288 (MQ=255)
                                                                                  aTAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCTGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCAGACCCAGAGCGGACTTTGGTTGTTTGACTGc                                                                                                                                                                                       >  2:1885456/1‑289 (MQ=255)
                                                                                  aTAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAAc                                                                                                                                                                                                                                 <  1:1065977/247‑1 (MQ=255)
                                                                                  aTAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGc                                                                                                                                                                                       >  2:1877063/1‑289 (MQ=255)
                                                                                       ggCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGa                                                                                                                                                                                  >  2:1437660/1‑289 (MQ=255)
                                                                                        gCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGa                                                                                                                                                                                  <  1:1633098/288‑1 (MQ=255)
                                                                                            ttCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGc                                                                                                                                                                              >  1:268230/1‑288 (MQ=255)
                                                                                            ttCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCa                                                                                                                                                                             <  1:1762817/289‑1 (MQ=255)
                                                                                                gCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCa                                                                                                                                                                         <  2:1356022/289‑1 (MQ=255)
                                                                                                   cGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCa                                                                                                                                                                      <  2:569788/289‑1 (MQ=255)
                                                                                                      caacaaACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTAACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCa                                                                                                                                                                   >  2:387129/1‑289 (MQ=255)
                                                                                                       acaaaacaCCGCGCACCGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAg                                                                                                                                                                  <  2:1051074/286‑1 (MQ=255)
                                                                                                              acCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGc                                                                                                                                                           >  1:1778903/1‑289 (MQ=255)
                                                                                                                cgcgCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCAt                                                                                                                                                         >  1:1285302/1‑289 (MQ=255)
                                                                                                                cgcgCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCAt                                                                                                                                                         >  2:867942/1‑289 (MQ=255)
                                                                                                                cgcgCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCAt                                                                                                                                                         >  1:454346/1‑289 (MQ=255)
                                                                                                                cgcgCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCAt                                                                                                                                                         >  1:961251/1‑289 (MQ=255)
                                                                                                                  cgcAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATa                                                                                                                                                        >  2:1794746/1‑288 (MQ=255)
                                                                                                                                aataaGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCACAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTg                                                                                                                                         <  1:900201/289‑1 (MQ=255)
                                                                                                                                 ataaGGCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGAACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTgg                                                                                                                                        >  2:714164/1‑289 (MQ=255)
                                                                                                                                    acgccgaaaGCTGAGAAACCGATAATGCCGAGCGATGCCGTTCTTGCCATTTAATCATAACGATCTCCTTTTTGA‑TGTATGCCTTATACTAGCCGGAATATATTTAGAAACGTGATGAACTCATGGCATTAATTTTTTTAGGTACTTCAGCAGACATGCCAACCCGCACGCGCAATGTCACGGCAATATTGCGTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGaaa                                                                                                                                     >  2:235241/7‑289 (MQ=255)
                                                                                                                                      gCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGCAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCCACATCCGACCCACAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTTCATACCGCCTTGTACCTTGGaaa                                                                                                                                     >  2:356536/1‑287 (MQ=255)
                                                                                                                                      gCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGTGGTTAAGGCACCCAGCAACAGCTAATGCATACCGCCTTTAAACCTGGaaa                                                                                                                                     >  2:1565429/1‑287 (MQ=255)
                                                                                                                                      gCATAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGaaa                                                                                                                                     >  2:634459/1‑287 (MQ=255)
                                                                                                                                         tAAAGCTGGGAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCATCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGa                                                                                                                                       <  2:1225411/282‑1 (MQ=255)
                                                                                                                                                gggAAACCGATAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCaa                                                                                                                                                                                                                                                                    >  2:909053/1‑150 (MQ=255)
                                                                                                                                                          tAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGt                                                                                                              <  2:779610/289‑1 (MQ=255)
                                                                                                                                                                 gagTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTc                                                                                                       >  1:250038/1‑289 (MQ=255)
                                                                                                                                                                             tCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGatc                                                                                              <  2:961251/286‑1 (MQ=255)
                                                                                                                                                                                tGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCttt                                                                                        >  1:843049/1‑289 (MQ=255)
                                                                                                                                                                                 gCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCagag                                                                                                                                                                                                             >  1:1866690/1‑172 (MQ=255)
                                                                                                                                                                                     ttCAATACTATTGCTCTCCTTTTGGA‑TGTATGCCGTATAATAACCGGAATATTTTTTGAAGCGGGATTAACTCATGGAATTAATTTTTTTAGGTACTTCCGCCGGTGTGCCAACCCGCACGCGACATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGt                                                                                   <  2:843049/289‑1 (MQ=255)
                                                                                                                                                                                      tCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGt                                                                                                              <  1:62147/261‑1 (MQ=255)
                                                                                                                                                                                      tCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGt                                                                                                              >  2:62147/1‑261 (MQ=255)
                                                                                                                                                                                      tCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGtt                                                                                  >  2:1023657/1‑289 (MQ=255)
                                                                                                                                                                                         aTCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTAc                                                                               <  1:1187827/289‑1 (MQ=255)
                                                                                                                                                                                          tCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATAATAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGACAat                                                                                                                                                                                                                                           >  2:1420105/1‑133 (MQ=255)
                                                                                                                                                                                              aaTGCGTTCCTTTTGGAGTGT‑TGCCTTATACAAGACGGAATATTTTTTGAAACGGTATGAACTCATGGAAGTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAAAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGc                                                                          <  2:1586942/289‑1 (MQ=255)
                                                                                                                                                                                                    ctcCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCtt                                                                        >  2:800696/1‑285 (MQ=255)
                                                                                                                                                                                                    ctcCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAACGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGt                                                                                                              <  2:530003/247‑1 (MQ=255)
                                                                                                                                                                                                    ctcCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCAGACCCAGAGCGGACTTTGGTTGTTTGATCGCGGTGAAGGCACCCAGCACCAGCTACTGCATACCGCCTTTAACCCTGGAAACCGGGACAAGCATTTTTA‑CCGTCACCTTCATGGCCATCATCTTTTTGGTTTCCCCGGCtt                                                                        >  2:233754/1‑285 (MQ=255)
                                                                                                                                                                                                     tcCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCtgt                                                                   >  1:1042403/1‑289 (MQ=255)
                                                                                                                                                                                                     tcCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCtgt                                                                   >  1:535391/1‑289 (MQ=255)
                                                                                                                                                                                                      ccTTTTTGA‑TGTATGCCTTATAATAGCCTGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCtgtg                                                                  <  2:150610/289‑1 (MQ=255)
                                                                                                                                                                                                         tttAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAg                                                               <  1:1125609/289‑1 (MQ=255)
                                                                                                                                                                                                          ttAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGt                                                              <  1:1226564/289‑1 (MQ=255)
                                                                                                                                                                                                                tGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTc                                                         <  2:118913/289‑1 (MQ=255)
                                                                                                                                                                                                                  tATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGTCGATCATCTTTTTGGTTTACCCGGCTGGCTGTGCAGGCGTTCtt                                                       >  2:153017/1‑288 (MQ=255)
                                                                                                                                                                                                                  tATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTa                                                       >  1:1314632/1‑289 (MQ=255)
                                                                                                                                                                                                                  tATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTa                                                       >  2:498554/1‑289 (MQ=255)
                                                                                                                                                                                                                  tATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTa                                                       >  2:439619/1‑289 (MQ=255)
                                                                                                                                                                                                                  tATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTa                                                       >  1:1879833/1‑289 (MQ=255)
                                                                                                                                                                                                                  tATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTa                                                       >  1:229774/1‑289 (MQ=255)
                                                                                                                                                                                                                  tATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTGTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGTCTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGt                                                                                                              <  2:1540604/234‑1 (MQ=255)
                                                                                                                                                                                                                    tGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTAc                                                                               >  2:1200228/1‑263 (MQ=255)
                                                                                                                                                                                                                    tGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTAc                                                                               <  1:1200228/263‑1 (MQ=255)
                                                                                                                                                                                                                     gCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATgt                                                    <  2:1314632/289‑2 (MQ=255)
                                                                                                                                                                                                                         tataCTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATgtcag                                                 >  1:554129/1‑284 (MQ=255)
                                                                                                                                                                                                                           taatggCCGGATTATTTGTAGAACAGTGATACACCGATGGCATTAATTTTTTTAGGTCAGTCAGCCAGTGTGCGAACCAGCACGCGCAATGTCACGGCAATATTGCTTAGCCTGCAACACCCGCCCCAGAGCGGACTTTGGTTGTTTGACTGTGGTGAAGGTCACAAGCATCAGCTACTGCAGACCGACTTTAACCCTGGAGAACTGGACAAG‑ATTTGTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATgtcaggca                                              <  2:1100513/284‑8 (MQ=255)
                                                                                                                                                                                                                           taCTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATgtcaggca                                              <  1:867942/289‑8 (MQ=255)
                                                                                                                                                                                                                           taCTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATgtcaggca                                              <  1:1348023/289‑8 (MQ=255)
                                                                                                                                                                                                                            aCTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGATTGCGGTGAAGGCCCCCAGCCTCAGCTACTGCATACCGCCTTTAACCCTGGAAACCAGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATgtcaggcat                                             >  1:410708/1‑281 (MQ=255)
                                                                                                                                                                                                                                gCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCCTCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATGTCAGGCATTAt                                          >  2:1118853/1‑288 (MQ=255)
                                                                                                                                                                                                                                gCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTg                                                                       <  1:1160931/259‑1 (MQ=255)
                                                                                                                                                                                                                                gCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCtg                                                                    >  2:1724779/1‑262 (MQ=255)
                                                                                                                                                                                                                                gCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATGTCAGGCATTAt                                          >  1:1935796/1‑288 (MQ=255)
                                                                                                                                                                                                                                gCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATGTCAGGCATTAt                                          >  1:1717718/1‑288 (MQ=255)
                                                                                                                                                                                                                                gCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATGTCAGGCATTAt                                          >  1:1685836/1‑288 (MQ=255)
                                                                                                                                                                                                                                 ccGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATGTCAGGCATTATcc                                        >  1:154405/1‑289 (MQ=255)
                                                                                                                                                                                                                                  cGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATGTCAGGCATTATcc                                        <  2:551834/288‑1 (MQ=255)
                                                                                                                                                                                                                                  cGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATGTCAGGCATTATc                                         <  1:797660/287‑1 (MQ=255)
                                                                                                                                                                                                                                     aaTATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCg                                                                                                                                                                                                           <  2:889328/123‑1 (MQ=255)
                                                                                                                                                                                                                                     aaTATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATGTCAGGCATTATCCAAc                                     >  1:745094/1‑288 (MQ=255)
                                                                                                                                                                                                                                     aaTATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAAATGGACAAG‑ATTTTTATCAGTCAACTTCATGGCGATCATCTTGTTGGTTTACCCGGCTTGCGGTTCAGTCGTTCTATGTCAGGCATTCTCCAAc                                     >  2:1855262/1‑288 (MQ=255)
                                                                                                                                                                                                                                           ttttGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATGTCAGGCATTATCCAACCCTTAAc                              <  1:758303/289‑1 (MQ=255)
                                                                                                                                                                                                                                                      gatgaACTCATGGAATTAAGTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATGTCAGGCATTATCCAACCCTTAACGATTTATGGTc                   <  1:18382/289‑1 (MQ=255)
                                                                                                                                                                                                                                                          cacccaatgAATTAATTTTTTTAGGTACTTCAGCCGGGGTGCCAACCCGCACGCGGAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATGTCAGGCATTATCCAACCCTTAACGATTTATGGTCCGCa               <  2:1646158/281‑1 (MQ=255)
                                                                                                                                                                                                                                                          aaCTCATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATGTCAGGCATTATCCAACCCTTAACGATTTATGGTCCGCa               <  2:481463/289‑1 (MQ=255)
                                                                                                                                                                                                                                                              cATGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATGTCAGGCATTATCCAACCCTTAACGATTTATGGTCCGCAAGGt           <  1:634459/289‑1 (MQ=255)
                                                                                                                                                                                                                                                               aTGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATGTCAGGCATTATCCAACCCTTAACGATTTATGGTCCGCAAGGTa          >  1:1487352/1‑289 (MQ=255)
                                                                                                                                                                                                                                                               aTGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATGTCAGGCATTATCCAACCCTTAACGATTTATGGTCCGCAAGGTa          >  2:750206/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                tGGAATTAATTTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCAAGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGCCTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCCTACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATCTTTATCAGTCACCTTCATGGCGACCATCTTTTTGTTGTACCCGGCTTGTTGTGCCGTCGTTCTTTTTCAGGCATTATCCAACCCTTAACTATTTATGGTcccgcaggtt          >  2:1751673/1‑280 (MQ=255)
                                                                                                                                                                                                                                                                    attaattTTTTTAGGTACTTCAGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACACAGCATAAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTGTTTGGTGTACCCGGCTTGCTGTGCAGTCGTTCTATGTCAGGCATTATCACACCCTTAACGATTTATGGTCCGCAATGTACCCGt     >  2:1339846/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                       |||                                                                                                                                                                                                                                                                               
TCGCATACGCCACCAGTTCATCATTTTTCCAACCGAGAATATGGCGATTATCCCCCGTCAAATCATCACCATCGATATCCTGATAAGGGCAATTCTGCTCGACAACAAACACCGCGCAACGCAGTTGTAATAAGGCATAAAGCTGGGAAACGCTTAATTCAGAGTGATGCAGATCTTGCCATTCAATCATAATGCTCTCCTTTTAGA‑TGTATGCCTTATACTAGCCGGAATATTTTTTGAAACGTGATGAACTCATGGAATTAATTTTTTTAGGTACTAGTGCCGGTGTGCCAACCCGCACGCGCAATGTCACGGCAATATTGCTTAACCTGCAACATCCGACCCAGAGCGGACTTTGGTTGTTTGACTGCGGTGAAGGCACCCAGCATCAGCTACTGCATACCGCCTTTAACCCTGGAAAACTGGACAAG‑ATTTTTATCAGTCACCTTCATGGCGATCATCTTTTTGGTTTACCCGGCTTGCTGTGCAGTCGTTCTATGAGTGGCATTATCCAACCCTTAACGATTTATGGTCCGCAAGGTATCCGTGAA  >  CP042583/1952642‑1953192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A