Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F210 I1 R1 4929 268.4 3907572 96.3% 3762991 273.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,958,324 3 bp→TCA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,958,3240AT99.2% 613.0 / NA 257intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (135/120);  minor base G (0/2);  total (135/122)
Fisher's exact test for biased strand distribution p-value = 2.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,958,3250GC99.6% 599.8 / NA 257intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (134/122);  minor base A (1/0);  total (135/122)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01
*CP0425831,958,3260TA99.2% 599.5 / NA 257intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (133/122);  minor base C (2/0);  total (135/122)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 257 total aligned reads displayed.

AGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACAGTGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTAGTGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAAAGTCTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCGGGAGTGACCTT  >  CP042583/1958047‑1958604
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                                         
agacagaTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACg                                                                                                                                                                                                                                                                                   <  1:312393/288‑1 (MQ=255)
 gacagaTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGcc                                                                                                                                                                                                                                                                                 >  1:353083/1‑289 (MQ=255)
 gacagaTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGc                                                                                                                                                                                                                                                                                  >  2:449547/1‑288 (MQ=255)
       ttGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGa                                                                                                                                                                                                                                                                               <  1:1020333/285‑1 (MQ=255)
       ttGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGa                                                                                                                                                                                                                                                                               <  1:1349293/285‑1 (MQ=255)
           aTTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGata                                                                                                                                                                                                                                                                        <  1:1738732/288‑1 (MQ=255)
                       tGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTg                                                                                                                                                                                                                                                           <  1:627712/289‑1 (MQ=255)
                         gCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTggg                                                                                                                                                                                                                                                         >  1:1455217/1‑289 (MQ=255)
                          cTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTggg                                                                                                                                                                                                                                                         <  1:1594754/288‑1 (MQ=255)
                                         gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTgg                                                                                                                                                                                                                                       >  1:438042/1‑289 (MQ=255)
                                         gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTgg                                                                                                                                                                                                                                       >  1:1821660/1‑289 (MQ=255)
                                         gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTgg                                                                                                                                                                                                                                       >  1:935131/1‑289 (MQ=255)
                                          tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATaaa                                                                                                                                                                                                                                                                <  2:409085/265‑1 (MQ=255)
                                          tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTgg                                                                                                                                                                                                                                       <  2:1821660/288‑1 (MQ=255)
                                          tgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATAAATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑AAACTTTggg                                                                                                                                                                                                                                      >  1:786939/1‑289 (MQ=255)
                                           tttATAACTTTGTGATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGc                                                                                                                                                                                                                                     <  1:1331082/288‑1 (MQ=255)
                                           gtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCGACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTggg                                                                                                                                                                                                                                      <  2:247295/288‑1 (MQ=255)
                                             tataACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTg                                                                                                                                                                                                                                   >  1:280094/1‑289 (MQ=255)
                                             tataACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTg                                                                                                                                                                                                                                   >  1:210517/1‑289 (MQ=255)
                                               taACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGAc                                                                                                                                                                                                                                 >  1:547982/1‑289 (MQ=255)
                                                    ttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGAc                                                                                                                                                                                                                                                                              >  1:929904/1‑241 (MQ=255)
                                                    ttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGc                                                                                                                                                                                                                             >  2:1770045/1‑288 (MQ=255)
                                                    ttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGc                                                                                                                                                                                                                             >  1:344331/1‑288 (MQ=255)
                                                    ttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGAAAGGc                                                                                                                                                                                                                             >  1:698454/1‑288 (MQ=255)
                                                    ttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTAGGATTTTTT‑‑ACACTTTGGGCTGACAGGc                                                                                                                                                                                                                             >  1:984266/1‑288 (MQ=255)
                                                    ttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGAGACATGCACATTTTATTTTCCCTCATCTGGTTTAAGATGCGAAACATGAAATTCCACGGAGGAAAAATAAATGGGAATGATTGGCTTTTTTGCAGAAATTGACTCAGAAAAGTTTAACCAAATTATTGAGTTTCATGAAAAACCTTTGAGGGAGAATATAAATGGAACGCCTTCAGGAGATCGCGGACTGGATATGGAGAAAAGCTGGGATTTTTT‑‑ACACTTTgggtggtgatgg                                                                                                                                                                                                                             >  2:1342006/1‑279 (MQ=255)
                                                     tGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCCTCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAAAAAATGGGAATGCTTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAAATGCTTGAGTCTACTGAAAAACCTTTGAAGGATACACACCATGACACGCTTTCAGGATTACGCCAACTGGATATTGATAACAGCTGTGATTTTTT‑‑ACCCTTTTGTCTTACCGGCa                                                                                                                                                                                                                            >  2:1796378/1‑288 (MQ=255)
                                                      gTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGAc                                                                                                                                                                                                                                                                              <  1:1079217/239‑1 (MQ=255)
                                                       ttATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACct                                                                                                                                                                                                                         <  2:693565/289‑1 (MQ=255)
                                                       ttATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACct                                                                                                                                                                                                                         <  2:173607/289‑1 (MQ=255)
                                                                 gTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTTTTTTAAGATGCGAAAACCGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTTATTCTACTGAAAAACCTTTGATGGCTAATATACATTACACGCTTTCCGGTTTACGCCCACCGGTTAATGATACAAGGCGGGATTTTTT‑‑AACTTTTGGGCTTACAGGCACCTCTGGCTTTTg                                                                                                                                                                                                               >  2:1918502/1‑289 (MQ=255)
                                                                   aaaTAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGAt                                                                                                                                                                                                             <  2:1133374/288‑1 (MQ=255)
                                                                       aataaAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAACAATAAATGGGAATGATTGGCTATTTTGCAGAACTTTACTCCGAAAAGATTAACCAACTGCTTGAGTCTACTTAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGGCTGGATATTTATTAACGCTGGGATTTTTT‑‑ACAATTTGGGTGGACAGGACCCTCT‑GCTTTTGATCCcg                                                                                                                                                                                                         >  2:732136/1‑288 (MQ=255)
                                                                       aataaAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCcgc                                                                                                                                                                                                        >  1:1210587/1‑289 (MQ=255)
                                                                       aataaAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCcgc                                                                                                                                                                                                        >  1:81102/1‑289 (MQ=255)
                                                                         taaAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTTACACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCcgc                                                                                                                                                                                                        >  1:491403/1‑289 (MQ=255)
                                                                         taaAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAAAATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTTCTTGAGTCTACTGAAAAACCTTTGATGGATAATATAAATGACACGGTTTCAGGATTACCACGACTGGATATTGATAAACGCAGGGATTTTTT‑‑AAACTTTGGGCGTACAGGCAACTCT‑GCTTTTGCTCTcgcca                                                                                                                                                                                                      >  2:481148/1‑287 (MQ=255)
                                                                               tttCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATg                                                                                                                                                                                                <  1:299339/289‑1 (MQ=255)
                                                                               tttCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATg                                                                                                                                                                                                <  1:1770045/289‑1 (MQ=255)
                                                                                                    aaaGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGATGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATGGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATCACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTcgtgccgtgc                                                                                                                                                                           <  2:1691203/289‑1 (MQ=255)
                                                                                                    aaaGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTcgtgccgtgc                                                                                                                                                                           <  2:1574848/289‑1 (MQ=255)
                                                                                                       gCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTg                                                                                                                                                                        >  2:835592/1‑289 (MQ=255)
                                                                                                              gCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAaca                                                                                                                                                                 <  2:796652/289‑1 (MQ=255)
                                                                                                              gCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAaca                                                                                                                                                                 <  1:1674824/289‑1 (MQ=255)
                                                                                                                     ttattttCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTc                                                                                                                                                          <  2:547982/289‑1 (MQ=255)
                                                                                                                              cTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACct                                                                                                                                                                                                                         >  1:1888854/1‑218 (MQ=255)
                                                                                                                                      ttttAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAaca                                                                                                                                                                 <  2:748403/265‑1 (MQ=255)
                                                                                                                                      ttttAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATgg                                                                                                                                         <  2:935131/289‑1 (MQ=255)
                                                                                                                                       tttAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATgg                                                                                                                                         <  1:449547/288‑1 (MQ=255)
                                                                                                                                       tttAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGAAATTGATAAACGCAGGGATTTATT‑‑AGACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGGGAAGAATGCTCCGTGGAGTCGAGCCGTGTTTGTAGAACACAACCTGGAAGATGGGATAGaag                                                                                                                                          >  2:1891097/1‑285 (MQ=255)
                                                                                                                                           aGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACGCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATCTTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGGTGGCta                                                                                                                                      >  2:36273/1‑286 (MQ=255)
                                                                                                                                           aGATGCGAAAAATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGTTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCACGCGAAGAATGCTCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTcc                                                                                                                                    <  1:1271257/289‑1 (MQ=255)
                                                                                                                                                cGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCGTCGAAGATCGCATTGATGGCTTCCTTGGa                                                                                                                               >  2:1320779/1‑289 (MQ=255)
                                                                                                                                                cGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCAGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGTGCTAGATGGCTGCCTTGGa                                                                                                                               >  2:1495537/1‑289 (MQ=255)
                                                                                                                                                 gAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAATGTTCACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGACGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGa                                                                                                                               <  1:470220/288‑1 (MQ=255)
                                                                                                                                                       tGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCt                                                                                                                        <  1:27459/289‑1 (MQ=255)
                                                                                                                                                       tGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCt                                                                                                                        <  1:1490008/289‑1 (MQ=255)
                                                                                                                                                       tGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCt                                                                                                                        <  1:262280/289‑1 (MQ=255)
                                                                                                                                                               aaCGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAgg                                                                                                                                                                                                                              >  1:745822/1‑180 (MQ=255)
                                                                                                                                                                  ggaggaAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAg                                                                                                             >  2:1073599/1‑289 (MQ=255)
                                                                                                                                                                          aataaatGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCcgc                                                                                                     <  2:1455217/289‑1 (MQ=255)
                                                                                                                                                                                    aaTGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATcga                                                                                            <  2:201120/288‑1 (MQ=255)
                                                                                                                                                                                        aTTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATcgaccg                                                                                         >  1:1918437/1‑287 (MQ=255)
                                                                                                                                                                                         gtgtgtATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGGTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGAGATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATcgaccgac                                                                                       <  1:732136/283‑1 (MQ=255)
                                                                                                                                                                                                ttttGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTTCGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGaatca                                                                               <  1:1866635/289‑4 (MQ=255)
                                                                                                                                                                                                  ttGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGaatc                                                                                >  2:127919/1‑284 (MQ=255)
                                                                                                                                                                                                  ttGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATAATGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGaatc                                                                                >  1:558382/1‑284 (MQ=255)
                                                                                                                                                                                                   tGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGaatcactg                                                                            <  2:53537/289‑7 (MQ=255)
                                                                                                                                                                                                      aGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGaatcactgga                                                                          <  2:81102/288‑9 (MQ=255)
                                                                                                                                                                                                          attgactcagAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGaa                                                                     >  2:428943/10‑289 (MQ=255)
                                                                                                                                                                                                                tcagAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAAc                                                               <  1:1233147/286‑1 (MQ=255)
                                                                                                                                                                                                                      aaGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGAGGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTa                                                         <  1:1844352/289‑1 (MQ=255)
                                                                                                                                                                                                                                   tGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCCCCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCCAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCAt                                            >  1:208056/1‑289 (MQ=255)
                                                                                                                                                                                                                                       tGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAg                                        <  1:835592/289‑1 (MQ=255)
                                                                                                                                                                                                                                        gAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGc                                       <  1:532314/289‑1 (MQ=255)
                                                                                                                                                                                                                                           tCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGa                                     <  2:1210587/288‑1 (MQ=255)
                                                                                                                                                                                                                                            cTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCTGACTGGATAATGATAAACGCTGGGATTGTTG‑‑ACACGTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGAGCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGCTGGCATAGATTGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACt                                   <  2:714788/289‑1 (MQ=255)
                                                                                                                                                                                                                                               cTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGa                                     >  2:645088/1‑284 (MQ=255)
                                                                                                                                                                                                                                               cTGAAAAACCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGAGCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTGaa                                >  2:926213/1‑289 (MQ=255)
                                                                                                                                                                                                                                                    aaaCCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCTGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTAACTGaaa                           >  2:1406021/1‑289 (MQ=255)
                                                                                                                                                                                                                                                    aaaCCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAAc                                                               >  2:281198/1‑253 (MQ=255)
                                                                                                                                                                                                                                                    aaaCCTTTGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGaaa                           >  1:1379886/1‑289 (MQ=255)
                                                                                                                                                                                                                                                       ccTTTGATGGATAATATACAGTACACGCTTTCACGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGTTCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATgg                        <  2:1874427/289‑1 (MQ=255)
                                                                                                                                                                                                                                                         tttGATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGGTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAACCAGTTTTCACTTAAGCGACCTAATGAAATGgat                      >  2:1666503/1‑288 (MQ=255)
                                                                                                                                                                                                                                                          ttGATGGATAATATACATGACACGCTTTCAGGATTATGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGgaga                     >  1:681319/1‑289 (MQ=255)
                                                                                                                                                                                                                                                            gATGGATAATATACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGAtt                   >  1:969429/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                 ataataTACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATc               <  1:525736/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                  taataTACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGGATCACTGGATCGAAATGAACTGCGTAAACAGTTTCCCATTAAGCGACTCAATGAAATGGAGATTTATCCg             >  2:1755179/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                  taataTACATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCg             >  2:338111/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                     tataCATGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGATGGGATTTGTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTCGCCGCGACAATCGACCGACTGGAATCACTGGATCGATATGAACTGCTTAAACAGTTTTCCATTAAGCGa                                     <  2:318097/262‑1 (MQ=255)
                                                                                                                                                                                                                                                                          aTGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTTGGCTGACAGGAACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCGGGAGTGAc     <  2:786939/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                          aTGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCCCTGGATCGAAATTAACTGCGTAAACAGTTTTCCATTAAGCGCCTCAATGAAATGGAGATTTATCCGGGAGtta      >  2:1378928/1‑286 (MQ=255)
                                                                                                                                                                                                                                                                          aTGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCGGGAGTGAc     <  2:35111/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                           tGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACACAATGAAATGGCGATTTATCCGGGAGTgc      >  2:1301863/1‑286 (MQ=255)
                                                                                                                                                                                                                                                                           tGACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAAAAGTTTTCCATGAAGCGACCAAATGAAATGGAGATTTATCCGGGAGTGAc     >  2:525736/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                            gACACGCTTTCAGGATTACGCCGACTGGATCTTGAAAACGGCTGGGATTTTTT‑‑ACACTTTGGTCTGACATGCACCTCT‑GCTTTAGATCCCGCGAAGAATGCTACGCTGAGGCGTGCCGTGCTTGGTGAACACAGCCTCGAAAATGGCATAGATGGCTTCCTTGGTTTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAAATGCGTAAACAGTTTTCCATTAAGCGAACCAATGAAATGGCGTTTTATACGGGAGTGACCt   >  2:890685/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                            gACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTaaa                                                       >  2:279768/1‑237 (MQ=255)
                                                                                                                                                                                                                                                                            gACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACt                                   >  2:378804/1‑257 (MQ=255)
                                                                                                                                                                                                                                                                            gACACGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACt                                   <  1:378804/257‑1 (MQ=255)
                                                                                                                                                                                                                                                                             acacGCTTTCAGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAATCACTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCGGGAGTGACCtt  >  1:1240639/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                                         
AGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACAGTGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGATGGATAATATACATGACACGCTTAGTGGATTACGCCGACTGGATATTGATAAACGCTGGGATTTTTT‑‑ACACTTTGGGCTGACAGGCACCTCT‑GCTTTTGATCCCGCGAAGAATGATCCGCTGAGTCGTGCCGTGCTTGGTGAACACAGCCTCGAAGATGGCATAGATGGCTTCCTTGGATTGACCTGGAATCAGGAGTTAGCCGCGACAATCGACCGACTGGAAAGTCTGGATCGAAATGAACTGCGTAAACAGTTTTCCATTAAGCGACTCAATGAAATGGAGATTTATCCGGGAGTGACCTT  >  CP042583/1958047‑1958604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A