Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F210 I1 R1 4928 368.7 6392010 97.5% 6232209 231.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 564,965 3 bp→TGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583564,9650AT99.7% 834.7 / NA 349intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (156/192);  minor base G (0/1);  total (156/193)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583564,9660CG99.7% 833.1 / NA 349intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base G (156/192);  total (156/193)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583564,9670TA99.7% 832.9 / NA 349intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (156/192);  minor base G (0/1);  total (156/193)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 349 total aligned reads displayed.

CCATGTCTGAAACTGGCTCTCTTCCGGTAACCCGGAATTATCTTCACATGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCACTTTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTACTGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACTTTCTCAAAGCCCAGCATTTCAAACAGCGCGTCGTACAGCGGGCGCAGATACGGGTCTACTT  >  CP042583/564693‑565239
                                                                                                                                                                                                                                                                                |||                                                                                                                                                                                                                                                                                
 taTGTCTGAAACTTGCTCGCGTCCGGTAACCCGGAATTATCTTCCACTGCCAGTTGTTATTCTAGGAGCACCTGTGTCATTTTTGTTCCTTTTCTTCGCTCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCGTCATAGGCGTTAACGATACGCTCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTAcgcg                                                                                                                                                                                                                                                                    <  2:70792/287‑1 (MQ=255)
 cATGTCTGAACCTGGCTCTCTTCCGGTAACCCGGAATTATCTTCACATGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTAcgcg                                                                                                                                                                                                                                                                    <  1:2198666/288‑1 (MQ=255)
   tGTCTGAAACTGGCTCTCTTCCGGTAACCCGGAATTATCTTCACATGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTAcg                                                                                                                                                                                                                                                                      >  1:3049507/1‑284 (MQ=255)
        gAAACTGGCTCTCTTCCGGTAACCCGGAATTATCTTCACATGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCg                                                                                                                                                                                                                                                            >  1:3171554/1‑289 (MQ=255)
          aaatgtacctctTCCTGTAACCCGGAATTATCTTCACATGCCATTTTTAACTCGATGATCACCTTACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGTCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGAt                                                                                                                                                                                                                                                          <  2:2941766/281‑1 (MQ=255)
          aaCTGGCTCTCTTCCGGTAACCCGGAATTATCTTCACATGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGAt                                                                                                                                                                                                                                                          <  1:720229/289‑1 (MQ=255)
               gCTCTCTTCCGGTAACCCGGAATTATCTTCACATGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGt                                                                                                                                                                                                                                                              >  2:1705694/1‑280 (MQ=255)
               gCTCTCTTCCGGTAACCCGGAATTATCTTCACATGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCtgtg                                                                                                                                                                                                                                                     >  2:582312/1‑289 (MQ=255)
                            aaCCCGGAATTATCTTCACATGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGt                                                                                                                                                                                                                                        <  1:582312/289‑1 (MQ=255)
                             aCCCGGAATTATCTTCACATGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCg                                                                                                                                                                                                                                             <  1:1819512/283‑1 (MQ=255)
                                        tCTTCACATGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCttt                                                                                                                                                                                                                            >  1:774886/1‑289 (MQ=255)
                                        tCTTCACATGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCttt                                                                                                                                                                                                                            <  2:1057956/289‑1 (MQ=255)
                                             acaTGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTAtt                                                                                                                                                                                                                                                                         <  1:1624757/239‑1 (MQ=255)
                                             acaTGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTAtt                                                                                                                                                                                                                                                                         >  2:1624757/1‑239 (MQ=255)
                                                  ccAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGAAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAgg                                                                                                                                                                                                                                                               <  1:415511/244‑1 (MQ=255)
                                                        gTAAATCGTGGATCCCCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACTCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATa                                                                                                                                                                                                            <  1:1752826/289‑1 (MQ=255)
                                                        gTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATa                                                                                                                                                                                                            <  2:2462731/289‑1 (MQ=255)
                                                        gTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATa                                                                                                                                                                                                            <  1:64188/289‑1 (MQ=255)
                                                            aTCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACggg                                                                                                                                                                                                        <  1:448351/289‑1 (MQ=255)
                                                                aGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAg                                                                                                                                                                                                    >  2:334893/1‑289 (MQ=255)
                                                                  gATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAgg                                                                                                                                                                                                   <  2:432444/288‑1 (MQ=255)
                                                                  gATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAgg                                                                                                                                                                                                   <  1:1490182/288‑1 (MQ=255)
                                                                   aTCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGa                                                                                                                                                                                                  <  1:1566811/288‑1 (MQ=255)
                                                                            cTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCa                                                                                                                                                                                         >  1:1097714/1‑288 (MQ=255)
                                                                               aTTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGAt                                                                                                                                                                                                                                                          <  1:1445715/220‑1 (MQ=255)
                                                                               aTTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCg                                                                                                                                                                                                                                             >  1:535333/1‑233 (MQ=255)
                                                                                         cTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGAt                                                                                                                                                                                                                                                          <  1:3156677/210‑1 (MQ=255)
                                                                                         cTGTTCTGCGGGCTTGCGTCCTGCTGGCAGCGCCGCTTTTCGGTTGTGTTCGGCTTCTTCCCGGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTGCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGAtggtg                                                                                                                                                                           <  2:3171554/289‑1 (MQ=255)
                                                                                              tttgtctcTTGCGTTGTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGtt                                                                                                                                                                      <  2:77271/282‑1 (MQ=255)
                                                                                                      ttGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGctc                                                                                                                                                               >  1:2899232/1‑288 (MQ=255)
                                                                                                             ctgctgCCAGCTCCGCTTGTCGTTTTTGTTCCGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCAAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTGCGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCgag                                                                                                                                                       <  2:2163247/289‑1 (MQ=255)
                                                                                                                    cAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGa                                                                                                                                                                                                  >  1:2004808/1‑239 (MQ=255)
                                                                                                                     aGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCttt                                                                                                                                                                                                                            <  1:101297/212‑1 (MQ=255)
                                                                                                                     aGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCttt                                                                                                                                                                                                                            >  2:101297/1‑212 (MQ=255)
                                                                                                                          cgcTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAAcgcg                                                                                                                                          <  1:1533159/289‑1 (MQ=255)
                                                                                                                            cTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGa                                                                                                                                                                                                  <  2:240381/231‑1 (MQ=255)
                                                                                                                               ttggTTTTTGTTTGGCTTTCTCCCAGGCTTCCTAGGCGTTAGGGATACGGGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCGCTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGtt                                                                                                                                     <  2:2578329/286‑1 (MQ=255)
                                                                                                                                        gTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCCCAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACg                                                                                                                            <  2:2306720/289‑1 (MQ=255)
                                                                                                                                        gTTCCGCTTCTTCCCAGGCTTCATAGGCGTTCACGATACGCGCCACACCAGGGTGACGAACCACGTCTTCGATGTGGAAGAAGTTAGAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTGGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACg                                                                                                                            <  2:2235517/289‑1 (MQ=255)
                                                                                                                                          tgggtttcttcCCAGGCTTCATTGGCGTTAACGTTACGCACCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGAGCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGAc                                                                                                                          <  2:1271090/284‑1 (MQ=255)
                                                                                                                                          tCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGAc                                                                                                                          <  1:366910/289‑1 (MQ=255)
                                                                                                                                             gCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCAc                                                                                                                       >  1:1848796/1‑289 (MQ=255)
                                                                                                                                              cttcttCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCAc                                                                                                                       <  1:2807880/288‑1 (MQ=255)
                                                                                                                                                        ggCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGcc                                                                                                            >  1:1713098/1‑289 (MQ=255)
                                                                                                                                                            tCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGAt                                                                                                                                                                                                                                                          <  2:1608275/143‑1 (MQ=255)
                                                                                                                                                            tCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGtt                                                                                                                                     >  1:1268381/1‑260 (MQ=255)
                                                                                                                                                              aTAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGct                                                                                                                                                                <  1:1609197/231‑1 (MQ=255)
                                                                                                                                                                  gCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACAGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACg                                                                                                                            <  1:2619717/263‑1 (MQ=255)
                                                                                                                                                                    gTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACt                                                                                                >  2:1303155/1‑289 (MQ=255)
                                                                                                                                                                     ttAACGATACGGGCCACCACAGGGGGACGAACCCCGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACtt                                                                                               <  2:1430019/289‑1 (MQ=255)
                                                                                                                                                                     ttAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGtt                                                                                                                                                                                   <  1:2689060/205‑1 (MQ=255)
                                                                                                                                                                     ttAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGtt                                                                                                                                                                                   >  2:2689060/1‑205 (MQ=255)
                                                                                                                                                                       aaCGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTaaaa                                                                                                                                                                                                                  >  1:2324283/1‑172 (MQ=255)
                                                                                                                                                                       aaCGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGct                                                                                                                                                                >  1:2382310/1‑222 (MQ=255)
                                                                                                                                                                            tACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGt                                                                                                                                                                                                       >  1:2772029/1‑178 (MQ=255)
                                                                                                                                                                            tACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGt                                                                                                                                                                                                       <  2:2772029/178‑1 (MQ=255)
                                                                                                                                                                            tACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAAc                                                                                                                                                                                                <  2:1978775/185‑1 (MQ=255)
                                                                                                                                                                            tACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACAGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATCAACGCGTCGCTCAGCGTACTACCACGCata                                                                                                                  >  2:2619717/1‑263 (MQ=255)
                                                                                                                                                                            tACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGATGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCata                                                                                                                  >  1:2948600/1‑263 (MQ=255)
                                                                                                                                                                             aCGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACg                                                                                       >  2:638569/1‑289 (MQ=255)
                                                                                                                                                                                         aGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATc                                                                           <  2:1325940/289‑1 (MQ=255)
                                                                                                                                                                                           ggTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGAt                                                                                                                                                                                                                                     <  2:2115517/133‑1 (MQ=255)
                                                                                                                                                                                           ggTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGAt                                                                                                                                                                                                                                     >  1:2115517/1‑133 (MQ=255)
                                                                                                                                                                                           ggTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAg                                                                         >  1:1051127/1‑289 (MQ=255)
                                                                                                                                                                                             tGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCa                                                                                                                        >  2:1182268/1‑240 (MQ=255)
                                                                                                                                                                                             tGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCAc                                                                                                                       >  2:3059173/1‑241 (MQ=255)
                                                                                                                                                                                             tGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGCCTTCACATACGCTGCGCTCAATCAGtt                                                                       >  2:476016/1‑289 (MQ=255)
                                                                                                                                                                                               aCGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCt                                                                                                                                                                    >  2:1549516/1‑194 (MQ=255)
                                                                                                                                                                                               aCGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGtt                                                                                                                                     <  1:1304183/225‑1 (MQ=255)
                                                                                                                                                                                               aCGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGtt                                                                       >  1:911721/1‑287 (MQ=255)
                                                                                                                                                                                                   aCCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCg                                                                  >  2:837488/1‑288 (MQ=255)
                                                                                                                                                                                                    acACGTTTTCGCTTTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCATTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGAc                                                                <  2:1657533/288‑1 (MQ=255)
                                                                                                                                                                                                     cACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCgag                                                                                                                                                       <  2:1477654/201‑1 (MQ=255)
                                                                                                                                                                                                       cGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATAc                                                                                                                                                                                                           >  1:1400542/1‑147 (MQ=255)
                                                                                                                                                                                                       cGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGCCtt                                                              >  1:2139837/1‑288 (MQ=255)
                                                                                                                                                                                                             cGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTAATCTGTTCGCTGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACACGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTCCGGCGTCAATAACGTTGCGCCCAATCAGTTTACCGACttgccgaaa                                                       >  2:515920/1‑282 (MQ=255)
                                                                                                                                                                                                                  tgGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATa                                                                                                                                                                                                            >  2:1812769/1‑135 (MQ=255)
                                                                                                                                                                                                                  tgGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACTTTCTCAAAGCCCa                                                  >  2:1253285/1‑289 (MQ=255)
                                                                                                                                                                                                                   ggAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACTTTCTCAAAGCCCa                                                  <  1:2421293/288‑1 (MQ=255)
                                                                                                                                                                                                                   ggAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACTTTCTCAAAGCCCa                                                  <  2:2899232/288‑1 (MQ=255)
                                                                                                                                                                                                                     aagaagTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACTTTCTCAAAGCCCAGc                                                <  2:1097714/288‑1 (MQ=255)
                                                                                                                                                                                                                            tAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCtttt                                                                                                                                                                                                                           >  2:540236/1‑109 (MQ=255)
                                                                                                                                                                                                                              aaGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACTTTCTCAAAGCCCAGCATTTCAAACa                                      <  2:3142352/289‑1 (MQ=255)
                                                                                                                                                                                                                                  tGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACTTTCTCAAAGCCCAGCATTTCAAACAgcgc                                  <  2:1853362/289‑1 (MQ=255)
                                                                                                                                                                                                                                  tGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACTTTCTCAAAGCCCAGCATTTCAAACAgc                                    <  1:837488/287‑1 (MQ=255)
                                                                                                                                                                                                                                  tGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACTTTCTCAAAGCCCAGCATTTCAAACAgc                                    <  1:2474400/287‑1 (MQ=255)
                                                                                                                                                                                                                                    aTCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTa                                                                                                                              <  2:2000490/195‑1 (MQ=255)
                                                                                                                                                                                                                                    aTCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGAcc                                                                                                                         <  2:2068500/200‑1 (MQ=255)
                                                                                                                                                                                                                                    aTCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGAcc                                                                                                                         >  1:2068500/1‑200 (MQ=255)
                                                                                                                                                                                                                                     tctcTTCGACATCGGCCAGCACTTCGATGGCGTGACGTATGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACTTTCTCAAAGCCCAGCATTTCAAACAGCGcgtc                               >  1:2553221/1‑289 (MQ=255)
                                                                                                                                                                                                                                       tctTCGACATCGGCCAGCACTTCGGTGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACTTTCTCAAAGCCCa                                                  <  1:3073817/268‑1 (MQ=255)
                                                                                                                                                                                                                                              cATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACTTTCTCAAAGCCCAGc                                                >  1:1190760/1‑263 (MQ=255)
                                                                                                                                                                                                                                                tCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACTTTCTCAAAGCCCAGCATTTCAAACAGCGCGTCGTACAGCGGgc                    <  1:1989078/289‑1 (MQ=255)
                                                                                                                                                                                                                                                tCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACTTTCTCAAAGCCCAGCATTTCAAACAGCGCGTCGTACAGCGGgc                    <  2:911721/289‑1 (MQ=255)
                                                                                                                                                                                                                                                 cGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACTTTCTCAAAGCCCAGCATTTCAAACAGCGCGTCGTACAGCGGgc                    >  1:16499/1‑288 (MQ=255)
                                                                                                                                                                                                                                                   gCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGAACTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACTTTCTCAAAGCCCAGCATTTCAAACAGCGCGTCGTACAGCGGGCGCa                 >  1:1689853/1‑289 (MQ=255)
                                                                                                                                                                                                                                                       tcACTTTGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGTGCGAGCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACTTTCTCAAAGCCCAGCATTTCAAACAGCGCGTCGTACAGCGGGCGCAGATa             <  2:251534/288‑1 (MQ=255)
                                                                                                                                                                                                                                                        cACGTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCCGTGATAACCGCTTTTGAGGTAAAACCGATACGGGTCAGGAACATCTTCCTCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCCACTTCACTAACGTTGCGCTCAATCGGTTTCTCGACTTTCTCAAACCCCATCATTTCAACCACGGCCTACAACAGCGGgccgaggtag            >  2:2376256/1‑281 (MQ=255)
                                                                                                                                                                                                                                                           ttCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCAc                                                                                                                       >  1:687472/1‑179 (MQ=255)
                                                                                                                                                                                                                                                                  gCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACg                                                                                                                            <  1:1926614/167‑1 (MQ=255)
                                                                                                                                                                                                                                                                                |||                                                                                                                                                                                                                                                                                
CCATGTCTGAAACTGGCTCTCTTCCGGTAACCCGGAATTATCTTCACATGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCACTTTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTACTGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACTTTCTCAAAGCCCAGCATTTCAAACAGCGCGTCGTACAGCGGGCGCAGATACGGGTCTACTT  >  CP042583/564693‑565239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A