Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F210 I1 R1 4927 250.2 4002508 96.7% 3870425 266.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 564,965 3 bp→TGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583564,9650AT99.1% 504.6 / NA 213intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (89/122);  minor base G (2/0);  total (91/122)
Fisher's exact test for biased strand distribution p-value = 1.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
*CP042583564,9660CG100.0% 504.1 / NA 213intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (91/122);  total (91/122)
*CP042583564,9670TA99.5% 498.8 / NA 213intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base A (90/122);  total (91/122)
Fisher's exact test for biased strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 213 total aligned reads displayed.

CATTCAGCCATGTCTGAAACTGGCTCTCTTCCGGTAACCCGGAATTATCTTCACATGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCACTTTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTACTGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACTTTCTCAAAGCCCAGCATTTCAAACAGCGCGTCGTACAGCGGGCGCAGATACGGGTCTACTTTCTG  >  CP042583/564686‑565243
                                                                                                                                                                                                                                                                                       |||                                                                                                                                                                                                                                                                                    
cATTCAGCCATGTCTGAAACTGGCTCTCTTCCGGTAACCCGGAATTATCTTCACATGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCTTGACGTAAGCCGGTTTTAGTa                                                                                                                                                                                                                                                                               >  2:348855/1‑289 (MQ=255)
     aGCCATGTCTGAAACTGGCTCTCTTCCGGTAACCCGGAATTATCTTCACATGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGCACTCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTAc                                                                                                                                                                                                                                                                           >  2:1273160/1‑288 (MQ=255)
         aTGTCTTAAACTGGCTCTCTTCCGGTAACCCGGAATTATCTTCACATGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGc                                                                                                                                                                                                                                                                      <  1:1632047/289‑1 (MQ=255)
               gacaCTGGCTCTCTTTCGTTAACCCATATTTTTTTTCACTGACCGTTTGTAAATCGCGGACCACCTGACTCATTTTTGTTCCTGTTCTTCCCGCTTTCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCg                                                                                                                                                                                                                                                                <  2:692445/286‑1 (MQ=255)
                 aaCTGGCTCTCTTCCGGTAACCCGGAATTATCTTCACATGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGa                                                                                                                                                                                                                                                               >  1:1793230/1‑288 (MQ=255)
                 aaCTGGCTCTCTTCCGGTAACCCGGAATTATCTTCACATGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGAt                                                                                                                                                                                                                                                              <  1:225348/289‑1 (MQ=255)
                 aaCTGGCTCTCTTCCGGTAACCCGGAATTATCTTCACATGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGAt                                                                                                                                                                                                                                                              <  2:839949/289‑1 (MQ=255)
                  aCTGGCTCTCTTCCGGTAACCCGGAATTATCTTCACATGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATc                                                                                                                                                                                                                                                             <  1:1329376/289‑1 (MQ=255)
                      gCTCTCTTCCGGTAACCCGGAATTATCTTCACATGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCtgtg                                                                                                                                                                                                                                                         >  2:1544938/1‑289 (MQ=255)
                      gCTCTCTTCCGGTAACCCGGAATTATCTTCACATGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCtgtg                                                                                                                                                                                                                                                         >  2:277655/1‑289 (MQ=255)
                      gCTCTCTTCCGGTAACCCGGAATTATCTTCACATGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCtgtg                                                                                                                                                                                                                                                         >  1:1497485/1‑289 (MQ=255)
                              ccGGTAACCCGGAATTATCTTCACATGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTc                                                                                                                                                                                                                                                  >  2:626780/1‑288 (MQ=255)
                                ggTAACCCGGAATTATCTTCACATGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGcc                                                                                                                                                                                                                                               >  1:659133/1‑289 (MQ=255)
                                  tAACCCGGAATTATCTTCACATGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCg                                                                                                                                                                                                                                              <  2:1855446/288‑1 (MQ=255)
                                      ccGGAATTATCTTCACATGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGAt                                                                                                                                                                                                                                         >  1:1462949/1‑289 (MQ=255)
                                          aaTTATCTTCACATGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAAc                                                                                                                                                                                                                                      <  2:659133/288‑1 (MQ=255)
                                               tCTTCACATGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCttt                                                                                                                                                                                                                                <  2:1454216/289‑1 (MQ=255)
                                                cTTCACATGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTAcgcg                                                                                                                                                                                                                                                                        <  2:728238/248‑1 (MQ=255)
                                                 ttCACATGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCttttg                                                                                                                                                                                                                              <  2:1262286/289‑3 (MQ=255)
                                                     caTGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCttttgagtt                                                                                                                                                                                                                          <  1:1963476/289‑7 (MQ=255)
                                                      aTGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGACGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGTTGATAACCGCttttgagtta                                                                                                                                                                                                                         >  1:1904317/1‑282 (MQ=255)
                                                       tGCCACTTGTAAATCGGGGATCACCTGCCTCATTTTTGTTCCTGTTCTTCGCTCTTGCGTCCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGATAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCttttgagttaa                                                                                                                                                                                                                        <  2:229648/289‑9 (MQ=255)
                                                             ttGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCg                                                                                                                                                                                                                                                 <  2:815298/258‑1 (MQ=255)
                                                               gTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATa                                                                                                                                                                                                                <  1:1001545/289‑1 (MQ=255)
                                                               gTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATa                                                                                                                                                                                                                <  2:1772763/289‑1 (MQ=255)
                                                               gTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATa                                                                                                                                                                                                                <  2:176286/289‑1 (MQ=255)
                                                               gTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATa                                                                                                                                                                                                                <  1:1544938/289‑1 (MQ=255)
                                                               gTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCATGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATa                                                                                                                                                                                                                <  1:942638/289‑1 (MQ=255)
                                                                tAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACCCGGAAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAACCCggaac                                                                                                                                                                                                               >  2:767027/1‑285 (MQ=255)
                                                                tAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTAAGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAATAGCTTTTGAGTTAAAACCgcttc                                                                                                                                                                                                               >  2:1119438/1‑285 (MQ=255)
                                                                     cGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTc                                                                                                                                                                                                          >  1:281940/1‑289 (MQ=255)
                                                                       aGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAg                                                                                                                                                                                                        >  2:516133/1‑289 (MQ=255)
                                                                       aGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAg                                                                                                                                                                                                        >  1:382546/1‑289 (MQ=255)
                                                                        ggATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATATGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAgg                                                                                                                                                                                                       >  2:866973/1‑289 (MQ=255)
                                                                          aTCACCTGATTCAGTGTTGTTCCTGTTGTGCGGGCTTGCGTGCTGCGGCCAGCGCCGGTGTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGa                                                                                                                                                                                                      <  2:306310/288‑1 (MQ=255)
                                                                          aTCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGa                                                                                                                                                                                                      <  1:516133/288‑1 (MQ=255)
                                                                                   cTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGAt                                                                                                                                                                                                                                                              <  2:1820297/223‑1 (MQ=255)
                                                                                   cTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGAt                                                                                                                                                                                                                                                              >  1:567748/1‑223 (MQ=255)
                                                                                   cTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGAt                                                                                                                                                                                                                                                              <  2:567748/223‑1 (MQ=255)
                                                                                             ttCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGAtg                                                                                                                                                                                  >  1:1048216/1‑289 (MQ=255)
                                                                                              tCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGTCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGACCAGCTTGATCTGTTCGAtgg                                                                                                                                                                                 >  2:1361844/1‑289 (MQ=255)
                                                                                              tCCTGTTCTGCGCGCTTGCGTTCTGGTGCCAGCGCCGCTTTTCGTTTTGGTGCGGCTTCTGCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGAtgg                                                                                                                                                                                 <  1:796220/289‑1 (MQ=255)
                                                                                                cTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGAtggtg                                                                                                                                                                               <  1:1566887/289‑1 (MQ=255)
                                                                                                     cttcGCGGTTGCGTTCTGCTGCCGTGGCCGTTTTTCGTTTTTGTTCTGCTTGTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCGTAGGGTGACGCACCATGTCTTCGCTGTGGAAGAAGTTAAAGCTGCTCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGATCATCTTCATCTGTTCGATGGTGGTGtt                                                                                                                                                                          <  2:1904317/289‑1 (MQ=255)
                                                                                                       tcGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGgtgt                                                                                                                                                                           >  1:820116/1‑286 (MQ=255)
                                                                                                        cgcgCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTc                                                                                                                                                                                      <  1:1583933/274‑1 (MQ=255)
                                                                                                           gcTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGct                                                                                                                                                                    <  1:1780549/289‑1 (MQ=255)
                                                                                                               gCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGa                                                                                                                                                                                    <  1:425278/269‑1 (MQ=255)
                                                                                                                    ctgctgCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCgag                                                                                                                                                           <  1:1104567/289‑1 (MQ=255)
                                                                                                                               gccgcTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATaa                                                                                                                                                   <  2:130796/286‑1 (MQ=255)
                                                                                                                               gccgcTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATaa                                                                                                                                                   <  1:1383996/286‑1 (MQ=255)
                                                                                                                                      ttCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGtt                                                                                                                                         <  1:307621/289‑1 (MQ=255)
                                                                                                                                      ttCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGtt                                                                                                                                         <  1:209067/289‑1 (MQ=255)
                                                                                                                                           ttttGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCg                                                                                                                                    <  1:972716/289‑1 (MQ=255)
                                                                                                                                               gTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACg                                                                                                                                <  1:235450/289‑1 (MQ=255)
                                                                                                                                                 tcgctattctcagtggCTTCAGAGATGTCAACGATCCGCGCCATCACATGGTTCTGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTATTCGACATCGGCCAGCACTTCGATGGCGTGGCGTAAGCCTGATTTTGTATTACGCGGCAGGTCGATCTGTTTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTATTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGAc                                                                                                                              <  2:1786157/275‑1 (MQ=255)
                                                                                                                                                   ggCTTGTTCCCAGGCTTCATAGGCGTTAACGATACGCGCAACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGGTCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGGAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCa                                                                                                                            <  2:24024/289‑1 (MQ=255)
                                                                                                                                                   ggCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCa                                                                                                                            <  2:1579497/289‑1 (MQ=255)
                                                                                                                                                     cttcttCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCAc                                                                                                                           <  1:873371/288‑1 (MQ=255)
                                                                                                                                                     cttcttCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCAc                                                                                                                           <  1:1119438/288‑1 (MQ=255)
                                                                                                                                                               ggCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAAcgcg                                                                                                                                              <  2:681812/259‑1 (MQ=255)
                                                                                                                                                                           gTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGAc                                                                                                     <  1:626780/288‑1 (MQ=255)
                                                                                                                                                                              aaCGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGa                                                                                                                                                                                                      >  1:1954256/1‑188 (MQ=255)
                                                                                                                                                                              aaCGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCa                                                                                                 >  1:874275/1‑289 (MQ=255)
                                                                                                                                                                                          caccacAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTgcg                                                                                      >  2:938699/1‑288 (MQ=255)
                                                                                                                                                                                                 gggTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGAtggtgg                                                                                                                                                                              >  1:348147/1‑193 (MQ=255)
                                                                                                                                                                                                 gggTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCa                                                                              >  2:10369/1‑289 (MQ=255)
                                                                                                                                                                                                  ggTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGtt                                                                                                                                         >  2:441531/1‑229 (MQ=255)
                                                                                                                                                                                                  ggTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGtt                                                                                                                                         <  1:441531/229‑1 (MQ=255)
                                                                                                                                                                                                  ggTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAAt                                                                                >  2:597976/1‑286 (MQ=255)
                                                                                                                                                                                                  ggTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAg                                                                             >  2:784851/1‑289 (MQ=255)
                                                                                                                                                                                                  ggTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCAGTGCGACTTCCATAACGTTGCGCTCAATCAg                                                                             >  2:1849765/1‑289 (MQ=255)
                                                                                                                                                                                                  ggTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGCCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCACTCAg                                                                             >  1:1280097/1‑289 (MQ=255)
                                                                                                                                                                                                   gTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATc                                                                               <  1:1699343/286‑1 (MQ=255)
                                                                                                                                                                                                    tGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGtt                                                                                                                                         >  2:1218902/1‑227 (MQ=255)
                                                                                                                                                                                                    tGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGtt                                                                                                                                         <  1:1218902/227‑1 (MQ=255)
                                                                                                                                                                                                      aCGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAAt                                                                                               >  1:595469/1‑267 (MQ=255)
                                                                                                                                                                                                      aCGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCAGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTg                                                                                                         >  2:873893/1‑257 (MQ=255)
                                                                                                                                                                                                       cGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCgaga                                                                                                                                                          >  1:453704/1‑207 (MQ=255)
                                                                                                                                                                                                            cACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACt                                                                   >  1:1412314/1‑289 (MQ=255)
                                                                                                                                                                                                              cGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGAc                                                                                                                              >  1:53754/1‑228 (MQ=255)
                                                                                                                                                                                                                         tgGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGGATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTc                                                                                   >  2:507117/1‑260 (MQ=255)
                                                                                                                                                                                                                         tgGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACTTTCTCAAAGCCCa                                                      >  1:1826157/1‑289 (MQ=255)
                                                                                                                                                                                                                          ggAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACTTTCTCAAAGCCCa                                                      <  1:675922/288‑1 (MQ=255)
                                                                                                                                                                                                                                 gTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATggtggt                                                                                                                                                                             >  2:1676754/1‑162 (MQ=255)
                                                                                                                                                                                                                                 gTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCCCAATCAGTTTCTCGACTTTCTCAAAGCCCAGCATTTCa                                              >  1:1841225/1‑289 (MQ=255)
                                                                                                                                                                                                                                  ttAATGCTGTTCTTTTCGACGTCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACTTTCTCAAAGCCCAGCATTTCaa                                             <  2:1841225/289‑1 (MQ=255)
                                                                                                                                                                                                                                     aaGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACTTTCTCAAAGCCCAGCATTTCAAACa                                          >  2:1967590/1‑289 (MQ=255)
                                                                                                                                                                                                                                          gATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTGCAAAAACGTTGCACCCAATCAGTTTCTCGACTTTCTCAAAGCCCAGCATTTCAAACAgaccg                                     >  2:865914/1‑285 (MQ=255)
                                                                                                                                                                                                                                           aTCTCTTCGACATCGGCCCGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCCCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACTTTCCCAACGCCCAGCATTTCAAACAgccct                                     >  2:1106780/1‑285 (MQ=255)
                                                                                                                                                                                                                                            tctcTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGc                                                                                                                 >  1:1740851/1‑211 (MQ=255)
                                                                                                                                                                                                                                            tctcTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGc                                                                                                                 <  2:1740851/211‑1 (MQ=255)
                                                                                                                                                                                                                                                   gACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACTTTCTCAAAGCCCAGCATTTCAAACAGCGCGTCGTACAGc                            <  1:549485/289‑1 (MQ=255)
                                                                                                                                                                                                                                                          gCCAGCACTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGa                                                                                                                                                                                                      <  2:1151755/112‑1 (MQ=255)
                                                                                                                                                                                                                                                            cAGCACTTCGATGGCGTGACGTAAGACTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAACCCGAAACGGGTCAGGAACATCTTCATCTGTTCTATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACTTTCTCAAAGCCCAGCATTTCAAACAGCGCGTCGTACAGCGGGCGCAGa                   <  1:176519/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                aCTTCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACTTTCTCAAAGCCCAGCATTTCAAACAGCGCGTCGTACAGCGGGCGCAGATa                 <  1:10369/287‑1 (MQ=255)
                                                                                                                                                                                                                                                                  ttCGATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACTTTCTCAAAGCCCAGCATTTCAAACAGCGCGTCGTACAGCGGGCGCAGATACggg             >  1:698452/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                     gATGGCGTGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACTTTCTCAAAGCCCAGCATTTCAAACAGCGCGTCGTACAGCGGGCGCAGATACGGGTCt          <  1:1995840/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                            tGACGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACTTTCTCAAAGCCCAGCATTTCAAACa                                          >  1:776242/1‑250 (MQ=255)
                                                                                                                                                                                                                                                                              aCGTAAGCCTGATTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTTGAGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCCCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACTTTCTCAAAGCCCAGCATTTCAAACAGCGCGTCGTACAGCGGGCGCAGATACGGGACTACTTTCTg  >  1:1486427/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                                       |||                                                                                                                                                                                                                                                                                    
CATTCAGCCATGTCTGAAACTGGCTCTCTTCCGGTAACCCGGAATTATCTTCACATGCCAGTTGTAAATCGAGGATCACCTGACTCATTTTTGTTCCTGTTCTTCGCGCTTGCGTTCTGCTGCCAGCGCCGCTTTTCGTTTTTGTTCGGCTTCTTCCCAGGCTTCATAGGCGTTAACGATACGCGCCACCACAGGGTGACGAACCACGTCTTCGCTGTGGAAGAAGTTAAAGCTGATCTCTTCGACATCGGCCAGCACTTCGATGGCGTGACGTAAGCCACTTTTAGTATTACGCGGCAGGTCGATCTGTGTGACGTCGCCGGTGATAACCGCTTTACTGTTAAAACCGATACGGGTCAGGAACATCTTCATCTGTTCGATGGTGGTGTTCTGGCTCTCATCGAGAATGATAAACGCGTCGTTCAGCGTACGACCACGCATATAGGCCAGCGGTGCGACTTCAATAACGTTGCGCTCAATCAGTTTCTCGACTTTCTCAAAGCCCAGCATTTCAAACAGCGCGTCGTACAGCGGGCGCAGATACGGGTCTACTTTCTG  >  CP042583/564686‑565243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A