Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F210 I1 R1 4928 368.7 6392010 97.5% 6232209 231.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 656,113 3 bp→TGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583656,1130AT99.3% 965.4 / NA 408intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/1);  major base T (208/195);  minor base G (0/3);  total (208/200)
Fisher's exact test for biased strand distribution p-value = 1.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583656,1140CG99.5% 964.5 / NA 408intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (205/200);  minor base T (2/0);  total (208/200)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583656,1150TA99.8% 975.1 / ‑5.0 408intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (207/199);  minor base G (0/1);  total (208/200)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01

Only 100 of 408 total aligned reads displayed.

AGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCACTAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAAGCTGGCGAGTCGCTCAGCCAGCTGCTGACGACTGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCGCTCATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGTT  >  CP042583/655835‑656393
                                                                                                                                                                                                                                                                                      |||                                                                                                                                                                                                                                                                                      
  cacaATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTGAGCCAGCTGCTGACGTGAGGCCACATcc                                                                                                                                                                                                                                                                              >  1:314132/1‑289 (MQ=255)
   acaATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATc                                                                                                                                                                                                                                                                               <  1:1379680/287‑1 (MQ=255)
       tGCGCGGTTCGTTGGCGGGTCAGGCGTGACGTGCTGAAGGCTCATCCGGCTCCGGAAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGCTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGAACGCCAGCAAACTGGACAACCTCAGGCATCTCATCGAACAGATTGAGCACCAGTAGCAGAGTAATACCGGACTGACATAACGAGCCGAGACACTCCACCGGCGTCTGACGTGCGGCAACAACCAGGca                                                                                                                                                                                                                                                                         >  2:1437616/1‑288 (MQ=255)
       tGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGcc                                                                                                                                                                                                                                                                         >  2:252583/1‑289 (MQ=255)
       tGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGcc                                                                                                                                                                                                                                                                         >  1:304888/1‑289 (MQ=255)
       tGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGc                                                                                                                                                                                                                                                                          >  1:1518154/1‑288 (MQ=255)
                 gTTGGGGGTTGAGGGGTGACGTTGTCAAGGTTCCTCCGGCTCTGGCAGTTGGCAACCTTTAAGCTGTTACCTCTGGGCCAGTTTCGGGACGGGTGCTTTGTGGGACAGTTCCTCATTTGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGGCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACgg                                                                                                                                                                                                                                                                <  2:2031831/288‑1 (MQ=255)
                        ggTAAGGCGTGACGTGCTGAAGGTTCATCTGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGTTCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATCCCGGACTGATGTAACGAGGCGAGTCGCTCAGCCACCTGCTGACGTTATGCAACATCCAGGCCATCGaagcgcccaacaa                                                                                                                                                                                                                                                        >  2:383643/1‑278 (MQ=255)
                                        ctgaaGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCCGACGTTAGGCAACATCCAGGCCATCGACCGCCCCATCAAGAATcacaaaatcac                                                                                                                                                                                                                                         >  2:1681938/5‑279 (MQ=255)
                                                tCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACCTCCAGGCCATCGAACGGCCCCTCCAGAAACAACCACTCAGgccaccaca                                                                                                                                                                                                                                >  2:3085206/1‑282 (MQ=255)
                                                tCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCACCGAACGGCGCATCAAGAATCAACAAGTCAGgctcccaca                                                                                                                                                                                                                                >  2:2228691/1‑284 (MQ=255)
                                                           cgggAGTTGCACACTTTTAAGCTATTTACTATGCGCCAGTTGCGGTACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGa                                                                                                                                                                                                                     <  2:2152457/289‑1 (MQ=255)
                                                                            cAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCCCCGACATCAGCGCCTGACACACCAGGGttttta                                                                                                                                                                                                     >  2:2697150/1‑287 (MQ=255)
                                                                                cTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGgctccgacatca                                                                                                                                                                                                                             <  1:412051/260‑9 (MQ=255)
                                                                                   ttCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATc                                                                                                                                                                                                                                                                               <  1:2269125/207‑1 (MQ=255)
                                                                                   ttCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCa                                                                                                                                                                                                                                                                        <  2:548871/214‑1 (MQ=255)
                                                                                   ttCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGtctc                                                                                                                                                                                              >  1:1125597/1‑288 (MQ=255)
                                                                                   ttCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTGTTTCGCGtctc                                                                                                                                                                                              >  1:2024709/1‑288 (MQ=255)
                                                                                   ttCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCACATCAAGAAGCAACAAGTCAGGCTCCGACATCAGCACCTGACACAGCAGGGgtttttgggcctc                                                                                                                                                                                              >  2:2763838/1‑280 (MQ=255)
                                                                                    tCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGc                                                                                                                                                                                            <  1:2228691/289‑1 (MQ=255)
                                                                                         aggggccgggtGCGGGCCGAGTGCTTGTTGGGGCGGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCGGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTCCGCGTCTCGCCAGTg                                                                                                                                                                                       <  2:2474488/279‑1 (MQ=255)
                                                                                          tGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCGGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACgg                                                                                                                                                                                                                                                                >  1:2101096/1‑215 (MQ=255)
                                                                                           gcgcCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGa                                                                                                                                                                                     <  1:2697150/289‑1 (MQ=255)
                                                                                             gcCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGgctccgacatca                                                                                                                                                                                                                             >  2:3173863/1‑239 (MQ=255)
                                                                                             gcCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGgctccgacat                                                                                                                                                                                                                               <  1:1885549/245‑7 (MQ=255)
                                                                                             gcCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGaaa                                                                                                                                                                                   <  1:2495775/289‑1 (MQ=255)
                                                                                              ccAGTTGCGAGACGAGTGCTTGTTGGAGCCGTTCCTCTTTAGCGCCAGTTGCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGGGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGaaa                                                                                                                                                                                   <  2:2916022/288‑1 (MQ=255)
                                                                                                       cgacgaGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGaa                                                                                                                                                                                                                                                                   <  2:1383435/199‑1 (MQ=255)
                                                                                                                   gttgGAGCAGTTCCTC‑TTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGCATTTCGCGTCTAGCCAGTGGAAAGGTATTTAAAGCGTCGGTAgagg                                                                                                                                                            >  1:1447162/1‑289 (MQ=255)
                                                                                                                      ggacacgTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCgaggag                                                                                                                                                          <  2:306688/283‑1 (MQ=255)
                                                                                                                      ggAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCgaggag                                                                                                                                                          <  1:1174445/289‑1 (MQ=255)
                                                                                                                              tCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCgg                                                                                                                                                     <  1:2557790/286‑1 (MQ=255)
                                                                                                                               ccTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGc                                                                                                                                                                                            >  1:1347776/1‑246 (MQ=255)
                                                                                                                               ccTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCGCGCACGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAAt                                                                                                                                                 >  2:1639081/1‑289 (MQ=255)
                                                                                                                                  ctTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCCCCTGACACAGCAGGGTTTTTCGCGTCCCGCCAGTGGAAAGGGATTTAAAGCGTCGGTCGGGGAGGGCGGTAATAc                                                                                                                                               >  2:816037/1‑288 (MQ=255)
                                                                                                                                     tAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCgaggag                                                                                                                                                          <  2:338401/274‑1 (MQ=255)
                                                                                                                                     tAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCgaggag                                                                                                                                                          >  1:338401/1‑274 (MQ=255)
                                                                                                                                      aGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAAc                                                                                                                                          <  1:555968/289‑1 (MQ=255)
                                                                                                                                       gcgcCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTa                                                                                                                                                                           <  1:711981/255‑1 (MQ=255)
                                                                                                                                               ttCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTgcgc                                                                                                                                  <  1:1727226/288‑1 (MQ=255)
                                                                                                                                                  cGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGGGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGGACTGCTGCGCCAGt                                                                                                                              >  1:2043689/1‑289 (MQ=255)
                                                                                                                                                      gaccgtgaaaccGCAAGAACGCAAGCAAACGTGGTAAACGTAGGGAACTCATAGAAGGGGTTGAGGACAAGGACCAGGGTAAGACCGGAAGGATGTAACGAGGCGGGTCGATCAGCCAGCTGCGGACGGGAGGCAACAGCCAGGCCATCGAACGGCTCATCAAGAATCAACGAGTCAGGGTCCGACATCAGGGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTtgc                                                                                                                           <  2:2624456/278‑1 (MQ=255)
                                                                                                                                                        cGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGGAATAACGAACTGCTGCCCCAGTtgcaa                                                                                                                         >  2:1198406/1‑287 (MQ=255)
                                                                                                                                                           gCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTtgcatgca                                                                                                                      <  1:1821441/288‑1 (MQ=255)
                                                                                                                                                             aaTCCGCCAGCACGGCAGCAAAAAGGACAAACTCCGGGATCGCATCGAAGCGAATGCGCACCAGGAACGGAGTTATGCCGGACTGATGTAAGGAGGCGGATCGCTCAGCCGGCTGCTAAGGAGAGGCAACATCCAGGCCATCCAACGGCTCATCAAGAATAAACAAGTCAGCCTCCGACATCAGGGCCTGACACAGCAGGGTTTTTGGCCTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAAcg                                                                                                                   <  2:314132/289‑1 (MQ=255)
                                                                                                                                                             aaTCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAAcg                                                                                                                   <  2:3041047/289‑1 (MQ=255)
                                                                                                                                                               tCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGgctccgacatca                                                                                                                                                                                                                             >  1:1012393/1‑173 (MQ=255)
                                                                                                                                                                 cgccagcacgccagcaAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGt                                                                                                               <  1:308420/289‑1 (MQ=255)
                                                                                                                                                                    cagcacgccagcaAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGc                                                                                                             <  1:2763838/288‑1 (MQ=255)
                                                                                                                                                                    caacaccccagcaAACTGGACAAACTCCGGGATCTCATCGAGGCGCTTGAGCCCCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCa                                                                                                            <  2:1125597/286‑1 (MQ=255)
                                                                                                                                                                                aaaCTGGACAAAATCAGGGATCTCATAGAAGCGAATGAGCACCAGTACCAGAGGAATAACGGACTGAGGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACt                                                                                                   <  2:2193654/286‑1 (MQ=255)
                                                                                                                                                                                   cTGGAACAACTCCGGGAGCTCATCGAAGCGATTGGGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATcc                                                                                             <  2:1775988/289‑1 (MQ=255)
                                                                                                                                                                                           aCTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCa                                                                                                            <  2:695469/266‑1 (MQ=255)
                                                                                                                                                                                             tCCGGGTTCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATCCCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGAtct                                                                                   <  1:942875/289‑1 (MQ=255)
                                                                                                                                                                                             tCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGAtct                                                                                   <  1:466229/289‑1 (MQ=255)
                                                                                                                                                                                             tCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGAtct                                                                                   <  1:2850864/289‑1 (MQ=255)
                                                                                                                                                                                                gggATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCa                                                                                 >  1:3178422/1‑288 (MQ=255)
                                                                                                                                                                                                       cATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGtt                                                                                                                             >  1:1936514/1‑237 (MQ=255)
                                                                                                                                                                                                       cATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGtt                                                                                                                             <  2:1936514/237‑1 (MQ=255)
                                                                                                                                                                                                       cATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGt                                                                         >  1:1487175/1‑289 (MQ=255)
                                                                                                                                                                                                         tCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTTATCCTGAATTATCCCAGCCGTAGttc                                                                       >  2:1341049/1‑287 (MQ=255)
                                                                                                                                                                                                                   tGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTc                                                             >  1:859535/1‑289 (MQ=255)
                                                                                                                                                                                                                       gacaggTACCGGAGTAATACCGGCAGGAAGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATc                                                          <  2:2931543/283‑1 (MQ=255)
                                                                                                                                                                                                                            gTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGtt                                                                                                                             >  2:1366590/1‑216 (MQ=255)
                                                                                                                                                                                                                            gTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCt                                                                                                        <  1:2449725/237‑1 (MQ=255)
                                                                                                                                                                                                                            gTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGa                                                                                          >  2:88848/1‑251 (MQ=255)
                                                                                                                                                                                                                              aCCAGAGTAATACCGGACGGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCTGGTGTCATCTTCGc                                                     <  2:2081508/286‑1 (MQ=255)
                                                                                                                                                                                                                               ccAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCa                                                                                                            >  1:2334328/1‑230 (MQ=255)
                                                                                                                                                                                                                                    gTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGa                                            >  2:429591/1‑289 (MQ=255)
                                                                                                                                                                                                                                       aTACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCgaggag                                                                                                                                                          <  2:638427/176‑1 (MQ=255)
                                                                                                                                                                                                                                       aTACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGAtct                                                                                   >  1:1414748/1‑247 (MQ=255)
                                                                                                                                                                                                                                       aTACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATc                                                          <  1:1001055/272‑1 (MQ=255)
                                                                                                                                                                                                                                       aTACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAggg                                                >  1:2931543/1‑282 (MQ=255)
                                                                                                                                                                                                                                        tACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGa                                                                                                                                                                                     >  1:2222055/1‑148 (MQ=255)
                                                                                                                                                                                                                                        tACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGc                                                                                                             <  1:1679472/220‑1 (MQ=255)
                                                                                                                                                                                                                                        tACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAg                                           <  1:59846/286‑1 (MQ=255)
                                                                                                                                                                                                                                         aCCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCata                                       <  1:2469252/289‑1 (MQ=255)
                                                                                                                                                                                                                                             gACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATcaac                                                                                                                                                                                                                                                >  2:938855/1‑84 (MQ=255)
                                                                                                                                                                                                                                             gACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGa                                                                                                                                                                                                                     >  2:2392072/1‑111 (MQ=255)
                                                                                                                                                                                                                                             gACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGt                                                                     <  1:1698128/255‑1 (MQ=255)
                                                                                                                                                                                                                                             gACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCgg                                   >  1:1233194/1‑289 (MQ=255)
                                                                                                                                                                                                                                              aCTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCatat                                      <  1:1297137/285‑1 (MQ=255)
                                                                                                                                                                                                                                                  atgtaacgaggCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCt                                                                                                        <  1:1073698/206‑1 (MQ=255)
                                                                                                                                                                                                                                                  atgtaacgaggCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATc                                                                                              >  1:3031789/10‑225 (MQ=255)
                                                                                                                                                                                                                                                       acgaggCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCgt                                                                                                                                                                     >  1:649146/5‑149 (MQ=255)
                                                                                                                                                                                                                                                       acgaggCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGt                                                                                                               <  1:292023/199‑1 (MQ=255)
                                                                                                                                                                                                                                                        cgaggCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCa                                                                                                            <  2:1163350/202‑1 (MQ=255)
                                                                                                                                                                                                                                                        cgaggCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCt                                                                                                        <  2:170078/206‑1 (MQ=255)
                                                                                                                                                                                                                                                        cgaggCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCCCAGCCGTAGTTCGTCCGGTTGCATCTTCGCCTGGGCCGAGCATATCGGGGTTTTTcctg                        >  2:1297137/4‑287 (MQ=255)
                                                                                                                                                                                                                                                        cgaggCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCCCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGCAATACCGAACTGCTGCGCCAGTTTCATGCACCGCGGGGCATCCTTTACTTCATCTTGAATGATCTCAGACGTCGTCCGTCCTGTTTCACCTTCGCCAGGGCCTACCAACTCGGGTTTAtttc                          >  2:1052106/4‑285 (MQ=255)
                                                                                                                                                                                                                                                          aggCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGTAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGAGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTTCGTCCGGTGTCCTCTTCTCCACGGCTGAGCATATCGTTGTTATTCCGCt                       >  1:814628/2‑288 (MQ=255)
                                                                                                                                                                                                                                                          aggCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGgtg                                                               >  2:24562/2‑248 (MQ=255)
                                                                                                                                                                                                                                                            gCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCgt                                                                                                                                                                     >  2:2352781/1‑144 (MQ=255)
                                                                                                                                                                                                                                                            gCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAg                                           <  1:2376208/266‑1 (MQ=255)
                                                                                                                                                                                                                                                            gCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGcc                    >  1:263539/1‑289 (MQ=255)
                                                                                                                                                                                                                                                            gCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGc                     >  2:1509321/1‑288 (MQ=255)
                                                                                                                                                                                                                                                            gCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTAGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCCACGCGGTGCATCCTTTACTTCATCCGAAATGATATCAGCCGTAGTTCGTCCGGTGGCATCTTCGCCAGGGCCGAGCATATCGgtt                                 >  2:1865697/1‑275 (MQ=255)
                                                                                                                                                                                                                                                            gCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCGCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGc                                                                                                             <  2:2278829/200‑1 (MQ=255)
                                                                                                                                                                                                                                                             cGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCa                                                                                                            <  2:1007559/200‑1 (MQ=255)
                                                                                                                                                                                                                                                                             ctgctgACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGt   >  1:2184631/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                      |||                                                                                                                                                                                                                                                                                      
AGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCACTAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAAGCTGGCGAGTCGCTCAGCCAGCTGCTGACGACTGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACAAGTCAGGCTCGCTCATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGACGAGTT  >  CP042583/655835‑656393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A