Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F210 I1 R1 4928 340.1 5480506 96.9% 5310610 252.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 656,113 3 bp→TGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583656,1130AT98.8% 751.7 / NA 320intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (155/161);  minor base G (0/4);  total (155/165)
Fisher's exact test for biased strand distribution p-value = 1.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583656,1140CG99.7% 753.7 / NA 320intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base G (154/165);  total (155/165)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583656,1150TA99.7% 764.1 / NA 320intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (154/165);  minor base C (1/0);  total (155/165)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 320 total aligned reads displayed.

CAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCACTAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAAGCTGGCGAGTCGCTCAGCCAGCTGCTGACGACTGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCGCTCATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGAC  >  CP042583/655834‑656388
                                                                                                                                                                                                                                                                                       |||                                                                                                                                                                                                                                                                                  
cAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGCCGCTCAACCAGCTTCTGACGTGAGGCAACa                                                                                                                                                                                                                                                                             >  2:2704907/1‑289 (MQ=255)
      aaTGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAgg                                                                                                                                                                                                                                                                       <  2:1288475/289‑1 (MQ=255)
       aggggtggttggttGGCGGGTAAGGCGTGACGGGCTGAAGGTCCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGGGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGc                                                                                                                                                                                                                                                                      <  2:1081059/283‑1 (MQ=255)
        tGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAgg                                                                                                                                                                                                                                                                       >  2:2003535/1‑287 (MQ=255)
        tGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGcc                                                                                                                                                                                                                                                                     >  1:1595757/1‑289 (MQ=255)
        tGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGcc                                                                                                                                                                                                                                                                     >  1:1404215/1‑289 (MQ=255)
        tGCGCGGCTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGcc                                                                                                                                                                                                                                                                     >  1:78436/1‑289 (MQ=255)
             ggTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGa                                                                                                                                                                                                                                                                >  1:1373671/1‑289 (MQ=255)
             ggTTCGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGATGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTGGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCAGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGAGTGAGCACCAGTACCAGAGTACTACCAGACTGATGTAACGAGGCGAGTCGCGCAGCCAGCTGCTGAGGTGCGGCAACATCCAGGCCATCGa                                                                                                                                                                                                                                                                >  1:1117646/1‑289 (MQ=255)
                 cGTTGGCGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACgg                                                                                                                                                                                                                                                            <  1:1221399/289‑1 (MQ=255)
                       cGGGTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCCCAGCCAGCTGCTGACGTGAGGCAACACCCAGGCCATCGAACGGCtcacc                                                                                                                                                                                                                                                      >  2:519356/1‑287 (MQ=255)
                         ggTAAGGCGTGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTCAGGCAACATCCAGGCCATCGAACGGCTCACCaa                                                                                                                                                                                                                                                    >  1:587620/1‑289 (MQ=255)
                                  tGACGTGCTGAAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATcaac                                                                                                                                                                                                                                            <  2:2231795/288‑1 (MQ=255)
                                    acgtgctgaaGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATcaaca‑a                                                                                                                                                                                                                                         >  1:753464/10‑288 (MQ=255)
                                     cgtgctgaaGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGt                                                                                                                                                                                                                                       <  1:493861/281‑1 (MQ=255)
                                         ctgaaGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCAAGGCCATGCAACGGCCCCTCAAGAATCAAAA‑AGTCAg                                                                                                                                                                                                                                    >  2:116433/5‑288 (MQ=255)
                                            aaGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCCCACCAAGAATCAACA‑AGGCAGgct                                                                                                                                                                                                                                 >  2:1637064/2‑288 (MQ=255)
                                                   aTCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGAACTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCAATCGAACGGCTCATCAAGAATCAACA‑AGTCAGgctccgacacc                                                                                                                                                                                                                         >  1:893446/1‑282 (MQ=255)
                                                                            tCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGttttt                                                                                                                                                                                                 >  1:2199106/1‑288 (MQ=255)
                                                                                  ttgtCACTAGGGGCCAGTTGTGCGAGGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGtct                                                                                                                                                                                          <  2:1229649/286‑1 (MQ=255)
                                                                                    ttCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGGTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGCTTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCAGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGtccc                                                                                                                                                                                         >  1:843933/1‑286 (MQ=255)
                                                                                              gcCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGACGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGCCGCGTCGCTCAGCCAGCTGCTGACGTGAGGCAACACCCAGGCCATCGACCGGCTCATCAAGAA‑CAACACATTCAGGCTCCCACCTTAGCGGCTGACACAGCAGGGACTTACGCATCTcccctgccgaaa                                                                                                                                                                              >  2:2594417/1‑281 (MQ=255)
                                                                                              gcCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGa                                                                                                                                                                                                                <  1:1706610/255‑1 (MQ=255)
                                                                                              gcCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGa                                                                                                                                                                                                                >  2:1706610/1‑255 (MQ=255)
                                                                                              gcCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGaaa                                                                                                                                                                              <  1:1851611/289‑1 (MQ=255)
                                                                                               ccAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGaaa                                                                                                                                                                              <  2:777474/288‑1 (MQ=255)
                                                                                                   ttGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGCTGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGCAGCAACA‑AGTCAGGCTCCGACACCAGCGCCTGACACAGCAGGGATTTTCGCGTCGGGCCAGGGGCAAGGTAt                                                                                                                                                                         >  2:983779/1‑289 (MQ=255)
                                                                                                   ttGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATAAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGaa                                                                                                                                                                               >  2:2159718/1‑283 (MQ=255)
                                                                                                   ttGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATAAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGaa                                                                                                                                                                               <  1:2159718/283‑1 (MQ=255)
                                                                                                          aagaGTGCTAGTTGGAGCGGTTCCCATATAGAGCACGTGTACGCGAAGGAGAATGCCGCCAGACAGCCAGCAAACTGGAGAAACTGCGGGATCTCATGCAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAg                                                                                                                                                                   <  2:361100/286‑1 (MQ=255)
                                                                                                                        gAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGt                                                                                                                                                                                            >  1:224055/1‑249 (MQ=255)
                                                                                                                         cgaaGTTCCTCGTAAGCGCCAGTTTCCGCTAACGTGCACTCCGGCAGCACGCCAGCAAACTGGACAACCGCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGGGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAggg                                                                                                                                                   <  2:1020862/286‑1 (MQ=255)
                                                                                                                         aGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGaca                                                                                                                                                                                                              >  2:638544/1‑230 (MQ=255)
                                                                                                                         aGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAggg                                                                                                                                                   >  2:2442845/1‑289 (MQ=255)
                                                                                                                              ttCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTa                                                                                                                                              >  1:642681/1‑289 (MQ=255)
                                                                                                                               tCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCgg                                                                                                                                                <  1:2564754/286‑1 (MQ=255)
                                                                                                                                   ctTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATAcc                                                                                                                                         >  1:1203625/1‑289 (MQ=255)
                                                                                                                                        gcgcCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGaa                                                                                                                                                                                                                                                               <  1:2076703/167‑1 (MQ=255)
                                                                                                                                        gcgcCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTc                                                                                                                                                                                                                                      >  1:1508038/1‑191 (MQ=255)
                                                                                                                                        gcgcCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCgag                                                                                                                                                        >  1:1039201/1‑269 (MQ=255)
                                                                                                                                        gcgcCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCgag                                                                                                                                                        <  2:1039201/269‑1 (MQ=255)
                                                                                                                                        gcgcCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGa                                                                                                                                       >  1:1431189/1‑286 (MQ=255)
                                                                                                                                         cgcCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAAct                                                                                                                                    >  2:1567106/1‑288 (MQ=255)
                                                                                                                                               tttCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTgcgc                                                                                                                             <  2:374912/289‑1 (MQ=255)
                                                                                                                                               tttCCGCTAACGGGCAATCCGCCCGAACGCCAGCAAACTGGACAAACTCCGGGATGTCATCGAAGCGATTGAGCACCAGTACCAGGGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTgcgc                                                                                                                             <  2:1896396/289‑1 (MQ=255)
                                                                                                                                                        aCGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCgaggag                                                                                                                                                     >  2:820636/1‑256 (MQ=255)
                                                                                                                                                         cGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTtgcatg                                                                                                                   <  1:2452220/289‑1 (MQ=255)
                                                                                                                                                            gCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTtgcatgca                                                                                                                 <  1:2379151/288‑1 (MQ=255)
                                                                                                                                                             cAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAAc                                                                                                               >  1:681300/1‑289 (MQ=255)
                                                                                                                                                              aaTCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAAcg                                                                                                              <  1:1811593/289‑1 (MQ=255)
                                                                                                                                                              aaTCCGCCAACACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAAcg                                                                                                              <  1:2598719/289‑1 (MQ=255)
                                                                                                                                                                  cgccagcacgccagcaAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTtgcatgca                                                                                                                 <  1:1052078/282‑1 (MQ=255)
                                                                                                                                                                    ccagcacgccagcaAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCCCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGc                                                                                                        <  1:1353320/289‑1 (MQ=255)
                                                                                                                                                                              agcaACATGGACAAGCACCGGGATCTCATCGAAGCGATTGAGCACCAGGACCAGAGAAATCCCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTTACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTGGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTTCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACt                                                                                              <  2:2146537/289‑1 (MQ=255)
                                                                                                                                                                                        aCAAACTCCGGTGACTCATCGAAGCGATTGAGCACCAAGCACAGAGTAATACCGGACGGAGGTAACGAGGCGAGTCGCTCAGCGAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGa                                                                                     <  2:2113619/288‑1 (MQ=255)
                                                                                                                                                                                        aCAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGa                                                                                     <  1:2704907/288‑1 (MQ=255)
                                                                                                                                                                                           aaCTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGa                                                                                 >  1:462585/1‑289 (MQ=255)
                                                                                                                                                                                            aCTCCGGGATCTCATCGAAGCGATTGAGCACCAGTAACAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGATGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGAt                                                                                <  1:2019237/289‑1 (MQ=255)
                                                                                                                                                                                              tCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGAtct                                                                              <  2:712475/289‑1 (MQ=255)
                                                                                                                                                                                              tCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGAtct                                                                              <  1:2410943/289‑1 (MQ=255)
                                                                                                                                                                                                     tatcATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGGGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGt                                                                       <  1:2616981/287‑1 (MQ=255)
                                                                                                                                                                                                          tCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGAATGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGAt                                                                                <  1:40868/275‑1 (MQ=255)
                                                                                                                                                                                                              aGCGCTTGAGCCCCATTACCAGAGTAATGCCTGAATGATGTAACGAGGCTGGTCGCGCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGGACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTc                                                               <  2:2666479/288‑1 (MQ=255)
                                                                                                                                                                                                              aGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTc                                                               <  1:2357287/288‑1 (MQ=255)
                                                                                                                                                                                                                   ttGAGCACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGtt                                                                                                                        >  2:1965450/1‑226 (MQ=255)
                                                                                                                                                                                                                        cACCAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATc                                                     <  2:1356873/288‑1 (MQ=255)
                                                                                                                                                                                                                           cAGTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTTTCATCTTc                                                  >  1:1260284/1‑288 (MQ=255)
                                                                                                                                                                                                                             gTACCAGAGTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAAcg                                                                                                              <  1:620177/226‑1 (MQ=255)
                                                                                                                                                                                                                                  agagTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAggg                                           >  1:992158/1‑288 (MQ=255)
                                                                                                                                                                                                                                     gTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCt                                                                                                   >  2:224466/1‑229 (MQ=255)
                                                                                                                                                                                                                                     gTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAggg                                           >  2:2538359/1‑285 (MQ=255)
                                                                                                                                                                                                                                     gTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAggg                                           <  1:2538359/285‑1 (MQ=255)
                                                                                                                                                                                                                                     gTAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTGTCATGCAACGCGGGGCATCCTTTACTTCATCCTGAATGATTCCAGCCGGAGTGCGTCCGGGGTcttt                                                     >  2:1499801/1‑272 (MQ=255)
                                                                                                                                                                                                                                      tAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGt                                                                                                          <  1:1756097/221‑1 (MQ=255)
                                                                                                                                                                                                                                      tAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGt                                                                                                          >  2:1756097/1‑221 (MQ=255)
                                                                                                                                                                                                                                      tAATACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGa                                       <  1:2669441/288‑1 (MQ=255)
                                                                                                                                                                                                                                       aaTACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAg                                      <  2:456225/288‑1 (MQ=255)
                                                                                                                                                                                                                                        aTACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGtt                                                                                                                        >  1:2140689/1‑205 (MQ=255)
                                                                                                                                                                                                                                        aTACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAAcg                                                                                                              >  1:1649050/1‑215 (MQ=255)
                                                                                                                                                                                                                                        aTACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGAtct                                                                              <  2:1828830/247‑1 (MQ=255)
                                                                                                                                                                                                                                        aTACCGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGAtct                                                                              >  1:1828830/1‑247 (MQ=255)
                                                                                                                                                                                                                                           ccGGACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCatat                                 <  1:825719/289‑1 (MQ=255)
                                                                                                                                                                                                                                              gACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCACGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGGATTTAAAGCGTCGGTCGAGGAGGGCGGCAATACCGAACTGCTGCGCCAGTTTCAGGCAACCCGGTGCATCCTTTACTTCCTCCGTAACTATCTCAGCCGCAGTGCGTCCGGGTTCATCTTTCCCAGTGCCCAgacaatcgc                              >  2:2380405/1‑281 (MQ=255)
                                                                                                                                                                                                                                              gACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAAcg                                                                                                              <  1:2331310/209‑1 (MQ=255)
                                                                                                                                                                                                                                              gACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAAcg                                                                                                              >  2:2331310/1‑209 (MQ=255)
                                                                                                                                                                                                                                              gACTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAAcg                                                                                                              <  1:1898402/209‑1 (MQ=255)
                                                                                                                                                                                                                                                cTGATGTAACGAGGCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGgtg                            >  1:2248918/1‑289 (MQ=255)
                                                                                                                                                                                                                                                   atgtaacgaggCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTgcgc                                                                                                                             >  2:946648/10‑189 (MQ=255)
                                                                                                                                                                                                                                                   atgtaacgaggCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATcc                                                                                        >  1:1969943/10‑226 (MQ=255)
                                                                                                                                                                                                                                                   atgtaacgaggCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGaa                                                                                    >  2:1422813/10‑230 (MQ=255)
                                                                                                                                                                                                                                                   atgtaacgaggCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGcc                                               >  1:1928871/10‑267 (MQ=255)
                                                                                                                                                                                                                                                   atgtaacgaggCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGcc                                               <  2:1928871/258‑1 (MQ=255)
                                                                                                                                                                                                                                                        acgaggCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGACTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGCGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCCTCCTTTACTTCATCCTGAATGACCTCAGCCTTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGACCATAACGGCGTTATTcc                     >  2:165415/5‑288 (MQ=255)
                                                                                                                                                                                                                                                         cgaggCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTcc                     >  1:557372/4‑287 (MQ=255)
                                                                                                                                                                                                                                                             gCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTgcgc                                                                                                                             <  2:867410/179‑1 (MQ=255)
                                                                                                                                                                                                                                                             gCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGc                <  1:2103292/288‑1 (MQ=255)
                                                                                                                                                                                                                                                             gCGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGCCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTAGTCcgccgc                >  1:355252/1‑288 (MQ=255)
                                                                                                                                                                                                                                                              cGAGTCGCTCAGCCAGCTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAAcg                                                                                                              <  2:2610531/193‑1 (MQ=255)
                                                                                                                                                                                                                                                                          ccagcTGCTGACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCCCTGCCATTCCTCGCTgtc  >  2:616379/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                              ctgctgACGTGAGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCCGACATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTAtt                       >  1:2177520/1‑264 (MQ=255)
                                                                                                                                                                                                                                                                                       |||                                                                                                                                                                                                                                                                                  
CAGCACAATGCGCGGTTCGTTGGCGGGTAAGGCGTGACGTGCACTAGGTTCATCCGGCTCCGGCAGTTGCACACCTTCAAGCTGTTCACTATGCGCCAGTTGCGCGACGAGTGCTTGTTGGAGCAGTTCCTCTTTAGCGCCAGTTTCCGCTAACGTGCAATCCGCCAGCACGCCAGCAAACTGGACAAACTCCGGGATCTCATCGAAGCGATTGAGCACCAGTACCAGAGTAATACCGGACTGATGTAAGCTGGCGAGTCGCTCAGCCAGCTGCTGACGACTGGCAACATCCAGGCCATCGAACGGCTCATCAAGAATCAACA‑AGTCAGGCTCGCTCATCAGCGCCTGACACAGCAGGGTTTTTCGCGTCTCGCCAGTGGAAAGGTATTTAAAGCGTCGGTCGAGGAGGGCGGTAATACCGAACTGCTGCGCCAGTTGCATGCAACGCGGTGCATCCTTTACTTCATCCTGAATGATCTCAGCCGTAGTGCGTCCGGTGTCATCTTCGCCAGGGCCGAGCATATCGGTGTTATTCCGCTGCCATTCGTCGCTGAC  >  CP042583/655834‑656388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A