Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F210 I1 R1 4928 368.7 6392010 97.5% 6232209 231.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 702,072 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583702,0720GC99.7% 728.6 / NA 306intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base C (160/144);  total (160/146)
Fisher's exact test for biased strand distribution p-value = 4.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583702,0730CG99.4% 720.5 / NA 306intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (2/0);  new base G (158/146);  total (160/146)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583702,0740TA99.7% 727.8 / NA 306intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (159/146);  minor base C (1/0);  total (160/146)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 306 total aligned reads displayed.

GGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGGC‑TTAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATACTGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGATTGCGATTAACTCGATACCCCAGTTTGGCATCGGCGACCCAGTAACCTTCGGTAAACGCTGGCGTTCCCACCGCG  >  CP042583/701792‑702347
                                                                                                                                                                                                                                                                                        ||||                                                                                                                                                                                                                                                                                 
    gCGTCCTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGcc                                                                                                                                                                                                                                                                          <  1:2536869/288‑1 (MQ=255)
        aCTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGa                                                                                                                                                                                                                                                                      <  2:620736/288‑1 (MQ=255)
        aCTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGa                                                                                                                                                                                                                                                                      <  1:664091/288‑1 (MQ=255)
            cgcCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCgg                                                                                                                                                                                                                                                                        >  1:373432/1‑282 (MQ=255)
            cgcCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAAtgtg                                                                                                                                                                                                                                                                 >  2:12285/1‑289 (MQ=255)
            cgcCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACCCCGGGAAtgtg                                                                                                                                                                                                                                                                 >  2:198114/1‑289 (MQ=255)
                 tCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTa                                                                                                                                                                                                                                                              >  1:2122886/1‑287 (MQ=255)
                 tCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTAca                                                                                                                                                                                                                                                            <  1:2675021/289‑1 (MQ=255)
                 tCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGGGGTAca                                                                                                                                                                                                                                                            >  1:1044719/1‑289 (MQ=255)
                 tCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATCCGGCTTGTTCCAGTTGTTCGCGTAAACTCGCGAAGTCC‑CTGGCCGATACCACGCCGGGCATGTGGtcat                                                                                                                                                                                                                                                            >  2:2945704/1‑286 (MQ=255)
                    acaTGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTAcatca                                                                                                                                                                                                                                                         >  1:915317/1‑289 (MQ=255)
                      aTGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTAcatcat                                                                                                                                                                                                                                                        >  1:1453051/1‑288 (MQ=255)
                           aaCCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGcc                                                                                                                                                                                                                                                                          <  2:2433571/265‑1 (MQ=255)
                           aaCCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGcc                                                                                                                                                                                                                                                                          >  1:2433571/1‑265 (MQ=255)
                           aaCCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGACGGACGTCCTGTGTCG‑ATAACACCCCGGGAATGTGGTACACCCTACACtt                                                                                                                                                                                                                                                  >  2:2881451/1‑289 (MQ=255)
                            aCCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAAtgtg                                                                                                                                                                                                                                                                 >  1:2018566/1‑273 (MQ=255)
                                tAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAAt                                                                                                                                                                                                                                                                    >  1:845556/1‑266 (MQ=255)
                                   gTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACAGCATACAATTTCTCCaaa                                                                                                                                                                                                                                          >  2:1622427/1‑289 (MQ=255)
                                      tCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAg                                                                                                                                                                                                                                       >  2:2635792/1‑289 (MQ=255)
                                       cATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGt                                                                                                                                                                                                                                      <  1:1622427/289‑1 (MQ=255)
                                              cTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTa                                                                                                                                                                                                                                                              >  1:411865/1‑258 (MQ=255)
                                              cTGAGAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTa                                                                                                                                                                                                                                                              <  2:411865/258‑1 (MQ=255)
                                                 aTAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTgc                                                                                                                                                                                                                             >  1:739698/1‑288 (MQ=255)
                                                  tAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTgcg                                                                                                                                                                                                                            <  1:12285/288‑1 (MQ=255)
                                                         tAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGcc                                                                                                                                                                                                                                                                          >  1:320811/1‑235 (MQ=255)
                                                                gcgcaggaaaaGGGTACGACGCAAGGCCGCGTTAAAGATTAAATCATGTTGGTTAACCCCGTTCAGCACCTCATTTTGCAGGGCGGCTGATAACGTGCTGCAGGTGTCGACCTGTTGAATGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTGCGCGAAAACTAGCGACTTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAAt                                                                                                                                                                                                              <  2:1292674/280‑1 (MQ=255)
                                                                     tGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGCATGTGGTACGTCATACAATTTCCCCAAAAAGTGGGGCCTGCCCCCCACATCTGAAACCGaaa                                                                                                                                                                                                        >  2:43391/1‑289 (MQ=255)
                                                                      ggAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGaaa                                                                                                                                                                                                        <  1:1319325/288‑1 (MQ=255)
                                                                            ggTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCatat                                                                                                                                                                                                 >  1:623458/1‑289 (MQ=255)
                                                                               aCGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATAttt                                                                                                                                                                                               >  1:2942632/1‑287 (MQ=255)
                                                                                 gcgGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCt                                                                                                                                                                                            <  1:141186/289‑1 (MQ=255)
                                                                                  cgGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAAt                                                                                                                                                                                                              <  2:1178811/270‑1 (MQ=255)
                                                                                  cgGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAAt                                                                                                                                                                                                              >  1:1178811/1‑270 (MQ=255)
                                                                                      aaGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGc                                                                                                                                                                                                                                 >  1:1762971/1‑247 (MQ=255)
                                                                                      aaGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACACCCGAATCAGAAATCAATATTTGCTGTTAg                                                                                                                                                                                       >  2:1811783/1‑289 (MQ=255)
                                                                                        tgCCACGGCAAGGATTAAAGCATCTTGGTCAACCGCGCTCAGCACCACACTTTGCAGACCGTCGTAAAACGAGCCTCGGTCGTCTACCCGTCGATCGCTCTTAACTAGCACACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCGCCACATCTGAACCAGACATGCATATTGGCTGGGAGCa                                                                                                                                                                                     >  2:1265052/2‑289 (MQ=255)
                                                                                           cgcgGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCt                                                                                                                                                                                            <  1:2290013/279‑1 (MQ=255)
                                                                                             cgGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCTGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCGGAATCAGAAATGCATATTGGCTGTGAGCaacaag                                                                                                                                                                                >  2:1885254/1‑289 (MQ=255)
                                                                                             cgGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCaagaag                                                                                                                                                                                >  2:3163430/1‑289 (MQ=255)
                                                                                                             aTGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGcc                                                                                                                                                                  <  1:1252424/287‑1 (MQ=255)
                                                                                                             aTGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGcc                                                                                                                                                                  <  1:2381218/287‑1 (MQ=255)
                                                                                                               gatGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCggg                                                                                                                                                              >  2:649984/3‑289 (MQ=255)
                                                                                                               gTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAACAGCATATTGGCTGTGAGCACGAAGATTCTTGGCTCGCCCggg                                                                                                                                                              >  2:2583203/1‑289 (MQ=255)
                                                                                                               gTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCCCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTTTGAGCAAAAAGGTTCTTGGCCCGCCCggg                                                                                                                                                              >  2:3086288/1‑289 (MQ=255)
                                                                                                                ttGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCg                                                                                                                                                                <  1:1472678/286‑1 (MQ=255)
                                                                                                                      aaCCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCcaca                                                                                                                                                                                                                     <  2:3010354/227‑1 (MQ=255)
                                                                                                                        ccGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGa                                                                                                                                                                                                                                                                      >  1:1134243/1‑176 (MQ=255)
                                                                                                                              tCAGCCCCTGATTGTGCAGGGGGGCGTATGACGTGCTGCGGGTGTCGGCCTGTGGATTGTTCTTAACTTGCGCGCCTTGTGCGCGGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGGGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCg                                                                                                                                               <  2:1408521/289‑1 (MQ=255)
                                                                                                                              tCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTgg                                                                                                                                                                                                                                    <  1:1000081/204‑1 (MQ=255)
                                                                                                                              tCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTgg                                                                                                                                                                                                                                    >  2:1000081/1‑204 (MQ=255)
                                                                                                                              tCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCg                                                                                                                                               <  1:2635792/289‑1 (MQ=255)
                                                                                                                                     cTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGccc                                                                                                                                                                                                                         >  2:250275/1‑208 (MQ=255)
                                                                                                                                       cATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGa                                                                                                                                                                                                                                                                      <  1:1899278/161‑1 (MQ=255)
                                                                                                                                               aGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACAt                                                                                                                                                                                                                    >  1:76240/1‑203 (MQ=255)
                                                                                                                                               aGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGAttgaggc                                                                                                                              >  1:2596692/1‑283 (MQ=255)
                                                                                                                                               aGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGAttgagg                                                                                                                               <  2:2026462/288‑6 (MQ=255)
                                                                                                                                                  gcggcgTATAACGTGCGTCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGAttgaggc                                                                                                                              <  1:2190005/286‑7 (MQ=255)
                                                                                                                                                   cggcgTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGAc                                                                                                                           <  1:2792766/288‑1 (MQ=255)
                                                                                                                                                       gTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAAt                                                                                                                      <  2:206858/289‑1 (MQ=255)
                                                                                                                                                       gTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAAt                                                                                                                      <  1:481966/289‑1 (MQ=255)
                                                                                                                                                        tataACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAAt                                                                                                                      <  1:1811783/288‑1 (MQ=255)
                                                                                                                                                         ataACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCg                                                                                                                    <  1:3086288/289‑1 (MQ=255)
                                                                                                                                                            aCGGGCTGCGGGTGTCGACCTGTTGATTGTTCTTAGCTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCAGTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTa                                                                                                                 <  2:1511260/289‑1 (MQ=255)
                                                                                                                                                                  tgcGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCt                                                                                                                                                                                            <  1:539930/208‑1 (MQ=255)
                                                                                                                                                                  tgcGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGTGAGCCGCCCTTGTTGATTGCGGCGACGTAACCGGTATCAAAc                                                                                                           >  2:895390/1‑289 (MQ=255)
                                                                                                                                                                  tgcGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAAc                                                                                                           >  2:511660/1‑289 (MQ=255)
                                                                                                                                                                    cGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAAGTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTGGTTGAGTGAGGCGACGGAAGCGGTATCAAACAg                                                                                                         >  2:2719121/1‑289 (MQ=255)
                                                                                                                                                                      ggTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCaaga                                                                                                                                                                                  <  1:1097736/214‑1 (MQ=255)
                                                                                                                                                                                            ttAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCg                                                                                                                                               >  1:2237772/1‑227 (MQ=255)
                                                                                                                                                                                            ttAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAAc                                                                                                           >  2:2240500/1‑263 (MQ=255)
                                                                                                                                                                                              aaCTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCg                                                                                <  1:298516/288‑1 (MQ=255)
                                                                                                                                                                                              aaCTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCg                                                                                >  2:298516/1‑288 (MQ=255)
                                                                                                                                                                                                 ttGCGCACCGTAGGGGCGGGGGGTGACGCGTCCATCCACCCATTCGGCTTGTGCCAGTTGTTCGCGAAAACGCGCGACGTCCTGTTGCG‑AAAGCACGCCGGGAATGTGGTACATCATTCAATTTCTCCAATGAGTGGTGCCTGCGCCCCACATCATAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGACGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGAt                                                                            <  2:358574/289‑1 (MQ=255)
                                                                                                                                                                                                  tGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGAtt                                                                           <  1:198114/289‑1 (MQ=255)
                                                                                                                                                                                                   gcgcACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTg                                                                                                                                                                                                                              >  1:718347/1‑141 (MQ=255)
                                                                                                                                                                                                   gcgcACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTg                                                                                                                                                                                                                              <  2:718347/141‑1 (MQ=255)
                                                                                                                                                                                                   gcgcACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGTTTCTTGTCTCGCCCGGGTGATAACGGTAGCCGCACTGGTTTATTGAGGCGACGTCAGCGGTATCAAACAGCTTGTAACCGTTTAGCTGGAGGtgtacatt                                                                           >  2:591127/1‑281 (MQ=255)
                                                                                                                                                                                                    cgcACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAGTCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGc                                                                                                                                                                   >  2:1084617/1‑199 (MQ=255)
                                                                                                                                                                                                    cgcACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGATTGc                                                                         >  2:781794/1‑289 (MQ=255)
                                                                                                                                                                                                           tgtgCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGt                                                                                                                  >  1:2098679/1‑241 (MQ=255)
                                                                                                                                                                                                           tgtgCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCa                                                                                                              <  1:1944438/245‑1 (MQ=255)
                                                                                                                                                                                                           tgtgCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCa                                                                                                              >  2:1944438/1‑245 (MQ=255)
                                                                                                                                                                                                                gcCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGCCGAGATTGCGATTAACTCGa                                                              <  1:2054184/288‑1 (MQ=255)
                                                                                                                                                                                                                  cGGGGGTGAACCGTCCATCCACCCCTTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGATTGCGATTAACTCGATAc                                                           <  2:739698/289‑1 (MQ=255)
                                                                                                                                                                                                                               tccatccaCCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGaaa                                                                                                                                                                                                        <  2:814177/135‑1 (MQ=255)
                                                                                                                                                                                                                               tccatccaCCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGaaa                                                                                                                                                                                                        >  1:814177/1‑135 (MQ=255)
                                                                                                                                                                                                                               tccatccaCCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAAt                                                                                                                      <  2:845362/217‑1 (MQ=255)
                                                                                                                                                                                                                               tccatccaCCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACg                                                                                               <  1:112343/240‑1 (MQ=255)
                                                                                                                                                                                                                               tccatccaCCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACg                                                                                               >  2:112343/1‑240 (MQ=255)
                                                                                                                                                                                                                               tccatccaCCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCgaga                                                                             >  1:1074739/1‑258 (MQ=255)
                                                                                                                                                                                                                               tccatccaCCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGAt                                                                            <  2:1366229/259‑1 (MQ=255)
                                                                                                                                                                                                                               tccatccaCCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGAt                                                                            >  1:1366229/1‑259 (MQ=255)
                                                                                                                                                                                                                                           ttCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGATTGCGATTAACTCGATACCCTCGTTTGGctccgcagacccctt                                  >  2:936024/1‑275 (MQ=255)
                                                                                                                                                                                                                                                  tGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCtgt                                                                                                                                                                                          <  2:2216996/130‑1 (MQ=255)
                                                                                                                                                                                                                                                     tCCAGTTGTTCGCGAAAACGAGCGACGTACTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGATTGCGATTAACTCGATACCCCAGTTTGGCATCGGCGACCCAGTAACCTTCGGt                        <  2:2110651/289‑1 (MQ=255)
                                                                                                                                                                                                                                                         gttgttCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAACCGTTTAGCTGGAAGTCGAGATTGCGATTAACTCGATCCCCAAGTTTGGCATCGGCGACCCAGGAACCCTCGGTAAAc                    >  2:1148600/1‑289 (MQ=255)
                                                                                                                                                                                                                                                         gttgttCGCGAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGAt                                                                            >  2:2792843/1‑233 (MQ=255)
                                                                                                                                                                                                                                                               cgcgAAAACGAGCGACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGATTGCGATTAACTCGATACCCCAGTTTGGc                                                <  2:1739187/255‑1 (MQ=255)
                                                                                                                                                                                                                                                                            gACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGATTGCGATTAACTCGATACCCCAGTTTGGCATCGGCGACCCAGTAACCTTCGGTAAACGCTGGCGTTCCCACcgcg  <  1:3052846/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                            gACGTCCTGTGGCG‑ATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGATTGCGATTAACTCGATACCCCAGTTTGGCATCGGCGACCCAGTAACCTTCGGTAAACGCTGGCGTTCCCACcgcg  <  1:2997474/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                        ||||                                                                                                                                                                                                                                                                                 
GGCTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGGC‑TTAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTGCGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATACTGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGATTGCGATTAACTCGATACCCCAGTTTGGCATCGGCGACCCAGTAACCTTCGGTAAACGCTGGCGTTCCCACCGCG  >  CP042583/701792‑702347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A