Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F210 I1 R1 4928 340.1 5480506 96.9% 5310610 252.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 702,072 3 bp→CGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583702,0720GC99.1% 748.0 / NA 318intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base C (169/146);  total (170/148)
Fisher's exact test for biased strand distribution p-value = 6.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583702,0730CG99.4% 745.2 / NA 318intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base G (169/147);  total (170/148)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583702,0740TA99.1% 745.3 / NA 318intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (1/0);  major base A (165/147);  minor base C (3/0);  total (170/148)
Fisher's exact test for biased strand distribution p-value = 2.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 318 total aligned reads displayed.

CTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGGCTTAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATACTGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGATTGCGATTAACTCGATACCCCAGTTTGGCATCGGCGACCCAGTAACCTTCGGTAAACGCTGGCGTTCCCACCGCGCCGT  >  CP042583/701794‑702351
                                                                                                                                                                                                                                                                                      |||                                                                                                                                                                                                                                                                                      
 ttcGTACTGCGCCATCCCCATGTGAGCCATAGGTTTAATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTAGGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGTCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCTACGTCCTGTGGCGATAACACGcc                                                                                                                                                                                                                                                                               <  2:985552/287‑1 (MQ=255)
 tGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGcc                                                                                                                                                                                                                                                                               >  2:1345874/1‑289 (MQ=255)
    gTACTGCGCCATCCACAGGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCgg                                                                                                                                                                                                                                                                             <  1:756875/288‑1 (MQ=255)
     tACTGCGCCATCCAAATGGAATCCATAGGTTTCATTGTTCTGAAAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGa                                                                                                                                                                                                                                                                           <  2:669749/289‑1 (MQ=255)
     tACTGCGCCAGCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGa                                                                                                                                                                                                                                                                           <  1:503366/289‑1 (MQ=255)
      gcTGCGCCATCCACATGGAAACAATAGGTTTAATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGa                                                                                                                                                                                                                                                                           <  2:2418360/287‑1 (MQ=255)
      atctcctcATCCACATGAAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAATAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGTCGTATAACGTGCTGCGCGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGa                                                                                                                                                                                                                                                                           <  1:881976/281‑1 (MQ=255)
      aCTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGa                                                                                                                                                                                                                                                                           <  1:856204/288‑1 (MQ=255)
          cgcCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTTGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGa                                                                                                                                                                                                                                                                           <  1:2325824/284‑1 (MQ=255)
          cgcCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACCCCGGGa                                                                                                                                                                                                                                                                           >  2:2325824/1‑284 (MQ=255)
             cATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTa                                                                                                                                                                                                                                                                   <  1:2720623/289‑1 (MQ=255)
               tcaccaTGGAAACGCTAGGTTTCATTGCTCTGAGAGCGATTAAACCGCGGCGTGGAAAGGGTCCGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTAca                                                                                                                                                                                                                                                                 <  2:2486383/285‑1 (MQ=255)
                          acaaTAGGTTTGATGGGTCTGGTAGCGATTAAACAGCGGGGGGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAAttt                                                                                                                                                                                                                                                      <  2:1219765/286‑1 (MQ=255)
                                  tttCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCaa                                                                                                                                                                                                                                                <  1:990832/287‑1 (MQ=255)
                                      aTTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTg                                                                                                                                                                                                                                          >  1:1682228/1‑289 (MQ=255)
                                      aTTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTg                                                                                                                                                                                                                                          >  1:2131000/1‑289 (MQ=255)
                                           tCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGc                                                                                                                                                                                                                                      <  1:699829/288‑1 (MQ=255)
                                               aTAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGa                                                                                                                                                                                                                                                                           >  2:1251592/1‑247 (MQ=255)
                                               aTAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGa                                                                                                                                                                                                                                                                           >  2:1162145/1‑247 (MQ=255)
                                               aTAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGa                                                                                                                                                                                                                                                                           <  1:1162145/247‑1 (MQ=255)
                                               aTAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGa                                                                                                                                                                                                                                                                           <  1:1251592/247‑1 (MQ=255)
                                               aTAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAAtg                                                                                                                                                                                                                                                                        >  2:1302759/1‑250 (MQ=255)
                                               aTAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGc                                                                                                                                                                                                                                      >  2:1560293/1‑284 (MQ=255)
                                                               ggcgTGGAAAGGGTACGCGGCACGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGAACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTTTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAAt                                                                                                                                                                                                                  <  1:944885/287‑1 (MQ=255)
                                                                               gcgGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCt                                                                                                                                                                                                <  1:1997659/289‑1 (MQ=255)
                                                                                    aaGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGa                                                                                                                                                                                                                                                                           >  1:541454/1‑210 (MQ=255)
                                                                                      ggCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAgaa                                                                                                                                                                                         >  1:1798080/1‑287 (MQ=255)
                                                                                                             gTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCggg                                                                                                                                                                  >  1:2225278/1‑289 (MQ=255)
                                                                                                             gTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCggg                                                                                                                                                                  >  1:1641674/1‑289 (MQ=255)
                                                                                                             gTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCggg                                                                                                                                                                  >  1:2648529/1‑289 (MQ=255)
                                                                                                             gTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGCCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCggg                                                                                                                                                                  >  2:892972/1‑289 (MQ=255)
                                                                                                             gTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGT‑‑‑‑‑GTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATa                                                                                                                                                             >  2:816999/1‑289 (MQ=255)
                                                                                                                   tAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATaa                                                                                                                                                            <  1:1927768/289‑1 (MQ=255)
                                                                                                                    aaCCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAAGGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTC‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGAAATAAGGTTCTTGGCTCGCCCGGTTggaaat                                                                                                                                                           >  2:734214/1‑284 (MQ=255)
                                                                                                                        gcgTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTTAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTa                                                                                                                                                       >  2:1596330/1‑289 (MQ=255)
                                                                                                                         cgTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCCCATCTGAATCAGAAATGCAAATTGGCTGTGACCAAGAAGGTTCTTGGCCCGCCCGGGTGTAAACGGtg                                                                                                                                                       >  2:1460451/1‑287 (MQ=255)
                                                                                                                         cgTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCGGAATCAGAAATGCATATTGGATGTGAGCAAGAAGGTTCTTGGCTAGCCGGGGTGATAACggga                                                                                                                                                       >  2:1175561/1‑286 (MQ=255)
                                                                                                                           ttCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGcc                                                                                                                                                                                                                                                                               >  1:1873615/1‑167 (MQ=255)
                                                                                                                           ttCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGcc                                                                                                                                                                                                                                                                               <  2:1873615/167‑1 (MQ=255)
                                                                                                                           ttCAGCACCCCATTTTGCAGGCCGGCGTATAACGTGCTGCGGGTGTCGCCCTGTTGATGGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGCCGCGTCCATCCACCCATTCGGCTTGTTCCAGTTTTTCGCGAAAACGAGCGACGTCCGTTGGCGATAACCCGCCGGGAATGGGTTAAATCATACAATTCCCCCAAAAA‑TGGGGGCTGCCGCCCACCACCCGAATCAGACATGCAAATTGGTTGTTGCAAAGAGGGTCCTGGCCCCGCCCGGGTgtaaccggaagcg                                                                                                                                                    >  2:44656/1‑277 (MQ=255)
                                                                                                                                  ccTCAGTTTGCAGGGCGGCGTATAACGTGTTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCttg                                                                                                                                             <  2:2649489/289‑1 (MQ=255)
                                                                                                                                     cATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCg                                                                                                                                                   <  1:2619980/280‑1 (MQ=255)
                                                                                                                                       ttttGCAGGGGGCCGAATAAGGTGCGGCGGGTGGCGACTTGTTGATTGTTCTTAACTTGCGCACCTGGTGGGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGAt                                                                                                                                        <  2:1798080/289‑1 (MQ=255)
                                                                                                                                          tGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGc                                                                                                                                                                                                 >  1:476890/1‑229 (MQ=255)
                                                                                                                                          tGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGc                                                                                                                                                                                                 <  2:476890/229‑1 (MQ=255)
                                                                                                                                                gcggcgTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGAc                                                                                                                               >  1:408674/1‑289 (MQ=255)
                                                                                                                                                  ggcgTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACg                                                                                                                              <  2:500209/288‑1 (MQ=255)
                                                                                                                                                    ggTATAACGTGCGGCGGGTGTCTGCCTGTTGATTTTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTa                                                                                                                            <  2:1924583/287‑1 (MQ=255)
                                                                                                                                                     gTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAAt                                                                                                                          <  1:1172683/289‑1 (MQ=255)
                                                                                                                                                      tataACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCAACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAAt                                                                                                                          <  2:330418/288‑1 (MQ=255)
                                                                                                                                                             tgctgcGTGTGTCGACCTGTTGATTGTTCTTCACTTGCGCACCTTGTGCGCCGGTGGTGCCGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTATGGCGATACCACGCCGGGGCTGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCCCATCTGAATCAGAATTGCATATTGGCTGTGCGCAAGACGCTTCTTGGATCGCCCGGGTGATAACGGCAGCCGCTCTTGTTGATTGCGCCGAGGTAATCGTTATcc                                                                                                                  >  2:386059/1‑288 (MQ=255)
                                                                                                                                                              gctgcGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGa                                                                                                                                                                                                                                                                           >  1:2024883/1‑136 (MQ=255)
                                                                                                                                                              gctgcGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGa                                                                                                                                                                                                                                                                           <  2:2024883/136‑1 (MQ=255)
                                                                                                                                                              gctgcGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCaa                                                                                                                 >  1:1000587/1‑289 (MQ=255)
                                                                                                                                                              gctgcGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCaa                                                                                                                 >  1:380030/1‑289 (MQ=255)
                                                                                                                                                                tgcGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCttg                                                                                                                                             <  1:1909222/259‑1 (MQ=255)
                                                                                                                                                                tgcGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCttg                                                                                                                                             >  2:1909222/1‑259 (MQ=255)
                                                                                                                                                                tgcGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCaaa                                                                                                                <  2:1682228/288‑1 (MQ=255)
                                                                                                                                                                tgcGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAAc                                                                                                               >  1:2580641/1‑289 (MQ=255)
                                                                                                                                                                tgcGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAAc                                                                                                               >  1:1504297/1‑289 (MQ=255)
                                                                                                                                                                tgcGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAAc                                                                                                               <  1:1345874/289‑1 (MQ=255)
                                                                                                                                                                  cGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAg                                                                                                             >  2:1633049/1‑289 (MQ=255)
                                                                                                                                                                         cGACCTGTTGTTTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTaa                                                                                                      <  2:2585513/289‑1 (MQ=255)
                                                                                                                                                                              tGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGtt                                                                                                 <  1:2506619/289‑1 (MQ=255)
                                                                                                                                                                                       ttCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGACGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTTAGGCGACGTAATCGGTCACAAACAGGTTGTAACCGTTTAGCTGGaa                                                                                        >  2:1444316/1‑289 (MQ=255)
                                                                                                                                                                                          ttAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGa                                                                                                                                                                                                              >  2:882023/1‑168 (MQ=255)
                                                                                                                                                                                          ttAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCAACATCTGATTCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTGGGCTCGCAGGGTTGATAACGGTAGCGGCTCTTGTTGATTGAGGCGACGTAACGGGTATAAAACAGGTTGTAAAAGTTGAGCTGGAAGta                                                                                     >  1:1454198/1‑288 (MQ=255)
                                                                                                                                                                                            aaCTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACCTCTGAATCAGa                                                                                                                                                                                                              >  1:1063399/1‑166 (MQ=255)
                                                                                                                                                                                            aaCTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACCTCTGAATCAGa                                                                                                                                                                                                              <  2:1063399/166‑1 (MQ=255)
                                                                                                                                                                                                 gcgcACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGt                                                                                                                                                        <  1:1539341/215‑1 (MQ=255)
                                                                                                                                                                                                 gcgcACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGCGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCgagagt                                                                               >  1:2082164/1‑286 (MQ=255)
                                                                                                                                                                                                 gcgcACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTACACGTTTAGCTGGAAGTCGAGAtt                                                                               >  2:164950/1‑288 (MQ=255)
                                                                                                                                                                                                  cgcACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGATTGc                                                                             >  1:1573715/1‑289 (MQ=255)
                                                                                                                                                                                                        tggtcgccaggtgatGAGGCTTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGATTGCGATTaa                                                                       <  2:139461/280‑1 (MQ=255)
                                                                                                                                                                                                         tgtgCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAg                                                                                                                                                                                           >  2:954941/1‑172 (MQ=255)
                                                                                                                                                                                                           tgCGCCGGTGGAGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTGCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGAAATGTGGTACATCCTACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGATTGCGATTAACt                                                                     <  1:64039/288‑1 (MQ=255)
                                                                                                                                                                                                                 ggtggtGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAAt                                                                                                                          <  1:1467835/229‑1 (MQ=255)
                                                                                                                                                                                                                 ggtggtGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGATTGCGATTAACTCGATAc                                                               >  1:2408832/1‑288 (MQ=255)
                                                                                                                                                                                                                 ggtggtGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCCGGAAGTCGAGATTGCGATTaaaccgaaac                                                               >  2:2711682/1‑280 (MQ=255)
                                                                                                                                                                                                                  gtggtgACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGATTGCGATTAACTCGATAcc                                                              >  1:353049/1‑288 (MQ=255)
                                                                                                                                                                                                                             tccatccaCCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAAt                                                                                                                                                                                                                  >  1:1107818/1‑129 (MQ=255)
                                                                                                                                                                                                                             tccatccaCCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAAt                                                                                                                                                                                                                  <  2:1107818/129‑1 (MQ=255)
                                                                                                                                                                                                                             tccatccaCCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCaaa                                                                                                                <  1:315807/227‑1 (MQ=255)
                                                                                                                                                                                                                             tccatccaCCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGATTGCGATTAACTCGATACCCCAGTTTGGc                                                    <  2:281945/287‑1 (MQ=255)
                                                                                                                                                                                                                             tccatccaCCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGATTGCGATTAACTCGATACCCCAGTTTGGCa                                                   >  1:1953505/1‑288 (MQ=255)
                                                                                                                                                                                                                                        aTTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATCACGGTAGCCGCTCTTGTTTATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAATGCGGGATTGCGATTAACTCGGAAACACAGTTTTGCAACGGCGACCAAg                                       >  2:2635019/1‑289 (MQ=255)
                                                                                                                                                                                                                                        aTTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAg                                                                                                                                                      >  2:218462/1‑178 (MQ=255)
                                                                                                                                                                                                                                        aTTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGGATCAAACCGGTTGTCAAAGTTTAGCTGTGAGTCGAAATTGCGATTAAATCGATACCCCAGTTTGGCATCGGCGACCCAg                                       >  2:2154289/1‑289 (MQ=255)
                                                                                                                                                                                                                                              cTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCaaa                                                                                                                <  2:1529073/210‑1 (MQ=255)
                                                                                                                                                                                                                                                tGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGATTGCGATTAACTCGATACCCCAGTTTGGCATCGGCGACCCAGTAACCTTc                               >  2:2078255/1‑289 (MQ=255)
                                                                                                                                                                                                                                                   tCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGATTGCGATTAACTCGATACCCCAGTTTGGCATCGGCGACCCAGTAACCTTCGGt                            <  1:2451824/289‑1 (MQ=255)
                                                                                                                                                                                                                                                   tCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGATTGCGATTAACTCGATACCCCAGTTTGGCATCGGCGACCCAGTAACCTTCGGt                            <  2:2122028/289‑1 (MQ=255)
                                                                                                                                                                                                                                                          gttCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGATTGCGATTAACTCGATACCCCAGTTTGGCATCGGCGACCCAGTAACCTTCGGTAAACGCt                     <  1:1633049/289‑1 (MQ=255)
                                                                                                                                                                                                                                                            tCGCGAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTc                                                                                     >  2:530099/1‑223 (MQ=255)
                                                                                                                                                                                                                                                             cgcgAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGATCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGTTAGCCGCTCTTGTTGTTTGAGTCGACGTAATCGGGATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGATTGCGTGTAACTAGCTACCCCAGTTTGGCCTCGGCTCCCCAGTAACCTTCGGTacactcgtgg                  >  2:1740866/1‑283 (MQ=255)
                                                                                                                                                                                                                                                             cgcgAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGATTGCGATTAACTCGATACCCCAGTTTGGCATCGGCGACCCAGTAACCTTCGGTAAACGCTgg                   >  2:640056/1‑288 (MQ=255)
                                                                                                                                                                                                                                                             cgcgAAAACGAGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGATTGCGATTAACTCGATACCCCAGTTTGGCATCGGCGACCCAGTAACCTTCGGTAAACGCTGGc                  >  1:1730700/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                       aGCGACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGAttgag                                                                                                                                    >  1:1305750/1‑161 (MQ=255)
                                                                                                                                                                                                                                                                          gACGTCCTGTGGCGATAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGATTGCGATTAACTCGATACCCCAGTTTGGCATCGGCGACCCAGTAACCTTCGGTAAACGCTGGCGTTCCCACcgcg      <  1:892972/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                          gACGTCCGGGGGGGAGAACACGCCGGGAATGTGGTACATCAGACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTAAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATTGAGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGATTGCGATTAACTCGATACCCCAGTTTGGCATCGGCGACCCAGTAACCTTCGGTAAACGCTGGCGTTCCCACcgcg      <  2:1004040/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                                      |||                                                                                                                                                                                                                                                                                      
CTGCGTACTGCGCCATCCACATGGAAACCATAGGTTTCATTGTTCTGATAGCGATTAAACAGCGGCGTGGAAAGGGTACGCGGCAAGGCCGCGGCAAAGAATAAAGCATGTTGGTTAACCGCGTTCAGCACCTCATTTTGCAGGGCGGCGTATAACGTGCTGCGGGTGTCGACCTGTTGATTGTTCTTAACTTGCGCACCTTGTGCGCCGGTGGTGACGCGTCCATCCACCCATTCGGCTTGTTCCAGTTGTTCGCGAAAACGAGCGACGTCCTGTGGGCTTAACACGCCGGGAATGTGGTACATCATACAATTTCTCCAAAAAGTGGGGCCTG‑CGCCCCACATCTGAATCAGAAATGCATATTGGCTGTGAGCAAGAAGGTTCTTGGCTCGCCCGGGTGATAACGGTAGCCGCTCTTGTTGATACTGGCGACGTAATCGGTATCAAACAGGTTGTAAACGTTTAGCTGGAAGTCGAGATTGCGATTAACTCGATACCCCAGTTTGGCATCGGCGACCCAGTAACCTTCGGTAAACGCTGGCGTTCCCACCGCGCCGT  >  CP042583/701794‑702351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A