Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F210 I1 R1 4928 368.7 6392010 97.5% 6232209 231.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,806,959 3 bp→TCG intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,806,9590AT98.8% 817.0 / NA 346intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (1/0);  major base T (164/176);  minor base G (3/1);  total (169/177)
Fisher's exact test for biased strand distribution p-value = 3.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,806,9600GC99.4% 810.2 / ‑2.6 346intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (1/0);  major base C (167/176);  minor base T (1/1);  total (169/177)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.15e-01
*CP0425831,806,9610CG98.8% 801.8 / NA 346intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (4/0);  new base G (163/177);  total (169/177)
Fisher's exact test for biased strand distribution p-value = 5.45e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 346 total aligned reads displayed.

ATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCAGTAGC‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑AGCAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGTGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGC  >  CP042583/1806679‑1807238
                                                                                                                                                                                                                                                                                          |||                                                                                                                                                                                                                                                                                     
atGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAAtttt                                                                                                                                                                                                                                                                                 >  2:1064686/1‑289 (MQ=255)
     aTTCAGATCACATATAATGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGt                                                                                                                                                                                                                                                                            >  1:2065114/1‑289 (MQ=255)
     aTTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCACCGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCCCTGGCGGCGGCCCGTCCCCGTACGCCATGT‑TCCAATTTTAATGt                                                                                                                                                                                                                                                                            >  2:2780658/1‑289 (MQ=255)
               caTATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTACAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAAt                                                                                                                                                                                                                                                                              >  2:561515/1‑277 (MQ=255)
                    aaaGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTgcgcg                                                                                                                                                                                                                                                             <  1:690023/289‑1 (MQ=255)
                    aaaGCCACAACGGGTTCGTAAAATGTTAACCCATTACATGATTATGAGGCAACGCCATGCATCCCCGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGAAAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAGGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGAAGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCAGTG‑TCGAATTTTAATGTTGGCGCAATTgcgcg                                                                                                                                                                                                                                                             <  2:1535043/289‑1 (MQ=255)
                           caACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGtgtg                                                                                                                                                                                                                                                      >  1:295121/1‑289 (MQ=255)
                                   tCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTgcgcgc                                                                                                                                                                                                                                                            <  1:1752660/275‑1 (MQ=255)
                                     gTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAACCCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTgcg                                                                                                                                                                                                                                                               <  1:3024472/270‑1 (MQ=255)
                                           tGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTgcgcgc                                                                                                                                                                                                                                                            <  1:1107983/267‑1 (MQ=255)
                                             ttATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTTTGACGGGCATCTGGTCtt                                                                                                                                                                                                                                      >  2:1998886/1‑287 (MQ=255)
                                             ttATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTc                                                                                                                                                                                                                                    >  1:950217/1‑289 (MQ=255)
                                             ttATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTc                                                                                                                                                                                                                                    >  2:2961347/1‑289 (MQ=255)
                                                     ttACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTgcgcg                                                                                                                                                                                                                                                             <  1:1139473/256‑1 (MQ=255)
                                                     ttACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGa                                                                                                                                                                                                                                                >  2:22285/1‑269 (MQ=255)
                                                     ttACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCaa                                                                                                                                                                                                                            >  2:251581/1‑289 (MQ=255)
                                                       aCATGATTATGAGGCAACGCCATGCATGCACGTTTTCAAACCGCTTTTGCCCATCTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACCCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAata                                                                                                                                                                                                                          <  2:1599193/289‑1 (MQ=255)
                                                       aCATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAata                                                                                                                                                                                                                          <  1:404737/289‑1 (MQ=255)
                                                       aCATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAACCTGCACTGGAACCTATTCTCGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCCCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCaa                                                                                                                                                                                                                            >  2:1969256/1‑287 (MQ=255)
                                                            aTTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAg                                                                                                                                                                                                                                                    >  1:2133908/1‑258 (MQ=255)
                                                                tGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTgcgcg                                                                                                                                                                                                                                                             <  2:710357/245‑1 (MQ=255)
                                                                tGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTa                                                                                                                                                                                                                 >  1:1292733/1‑289 (MQ=255)
                                                                 gAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTAt                                                                                                                                                                                                                <  1:2808946/289‑1 (MQ=255)
                                                                  aGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTAt                                                                                                                                                                                                                <  2:1863939/288‑1 (MQ=255)
                                                                     cAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTgg                                                                                                                                                                                                             <  1:2898808/288‑1 (MQ=255)
                                                                        cGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTgcgcgc                                                                                                                                                                                                                                                            >  1:1809070/1‑238 (MQ=255)
                                                                        cGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTgcgcgc                                                                                                                                                                                                                                                            <  2:1809070/238‑1 (MQ=255)
                                                                             tGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAata                                                                                                                                                                                                                          >  2:646309/1‑267 (MQ=255)
                                                                                   cACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATg                                                                                                                                                                                                                        <  2:3092400/263‑1 (MQ=255)
                                                                                    aCGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCaa                                                                                                                                                                                             >  2:1194578/1‑289 (MQ=255)
                                                                                    aCGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCCAATTTTAATGTTGGCGCCATTCCGCGCGGCTTGAGCGGAACCTGGTATTTCGGTGCCAATATGCCACTTATTGGTGCGACAATCAAGCaa                                                                                                                                                                                             >  2:2590307/1‑289 (MQ=255)
                                                                                        tttCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCCTTG‑TCCAATTTTAATGTTGGCGCAAGTGCGCGCGGTGTCAGGGGAACGTGGGATTTCGGTGCCAATATGGAATTTAGTGGTGCTTAAATCCAGCAAAACg                                                                                                                                                                                         >  2:2362126/1‑289 (MQ=255)
                                                                                         ttCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGt                                                                                                                                                                                        <  1:2362126/289‑1 (MQ=255)
                                                                                                 gCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCg                                                                                                                                                                                >  1:531207/1‑289 (MQ=255)
                                                                                                  cTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGa                                                                                                                                                                               >  1:662155/1‑289 (MQ=255)
                                                                                                              ttGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGAt                                                                                                                                                                   <  1:1340836/289‑1 (MQ=255)
                                                                                                                  ggATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGc                                                                                                                                                               <  1:2232265/289‑1 (MQ=255)
                                                                                                                    ttAACTTGCAATCTGCACTGGAACCTATTCTGGCAAACAAGTACTTCCCCGGTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGcc                                                                                                                                                              <  2:2672748/287‑1 (MQ=255)
                                                                                                                    aTAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGcc                                                                                                                                                              <  1:2709020/288‑1 (MQ=255)
                                                                                                                    aTAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCACACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAg                                                                                                                                                                                                                                                    <  1:1881608/202‑1 (MQ=255)
                                                                                                                    aTAACGTGAAAGCTGCACTGTAACATATTCTGGCAGACAAGTACTTCTCCGCTTTGTTGACCGGGGAGAAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGAGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGcc                                                                                                                                                              <  2:2433159/288‑1 (MQ=255)
                                                                                                                       aCTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACg                                                                                                                                                           >  2:1330805/1‑288 (MQ=255)
                                                                                                                                cTGCACTGGAACCTAGTCTGGCGGACAATTACTTCCCCGCTTTGTTGACGGGGGAGCAAGTGTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAGGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTTCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAg                                                                                                                                                 <  2:231443/289‑1 (MQ=255)
                                                                                                                                  gCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGc                                                                                                                                                                                                                               >  1:12314/1‑209 (MQ=255)
                                                                                                                                  gCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGc                                                                                                                                                                                                                               <  2:12314/209‑1 (MQ=255)
                                                                                                                                   cACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAg                                                                                                                                                                                                                                                    <  2:778223/187‑1 (MQ=255)
                                                                                                                                   cACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAg                                                                                                                                                                                                                                                    >  1:778223/1‑187 (MQ=255)
                                                                                                                                   cACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACACTGCCTCAAACCGTTCATTCCGGACAAAGCGCGCTAAGCCACGCCTGGTGGAgtgg                                                                                                                                              >  2:674008/1‑289 (MQ=255)
                                                                                                                                   cACTGGAACCTATCCTGGCAGACACATATTTCCCGGGTTTGTTGACCGGGGGGCAAGTCTCATCG‑CTGAAGAGGGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAgtg                                                                                                                                               <  2:1475912/288‑1 (MQ=255)
                                                                                                                                        gAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAg                                                                                                                                                                                                                                                    <  2:945799/182‑1 (MQ=255)
                                                                                                                                              aTTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGa                                                                                                                                                                                                        >  1:2982468/1‑220 (MQ=255)
                                                                                                                                               ttCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAg                                                                                                                                                                                                                                                    >  2:1142711/1‑175 (MQ=255)
                                                                                                                                                  tGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTc                                                                                                                                                                                      <  1:3067509/234‑1 (MQ=255)
                                                                                                                                                    gCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCa                                                                                                                              >  1:2592190/1‑288 (MQ=255)
                                                                                                                                                       gACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGgcg                                                                                                                                                                                                                                                                       <  1:3195559/148‑1 (MQ=255)
                                                                                                                                                         cAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCa                                                                                                                          <  2:2541338/287‑1 (MQ=255)
                                                                                                                                                               cTTCCCCTGTTTGTTGACCTGGAAGCAAGTC‑TCAGCGCTGAAAGGCGAAGCGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTGCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGtt                                                                                                                  <  2:1292733/289‑1 (MQ=255)
                                                                                                                                                               cTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGtt                                                                                                                  <  1:1982384/289‑1 (MQ=255)
                                                                                                                                                               cTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGtt                                                                                                                  <  1:714710/289‑1 (MQ=255)
                                                                                                                                                               cTTCCCCGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGtt                                                                                                                  <  1:480519/289‑1 (MQ=255)
                                                                                                                                                                     cGCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTAGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTac                                                                                                            <  1:1360875/289‑1 (MQ=255)
                                                                                                                                                                      gCTTTGTTGACCGGGGAGCAAGTCTCATCG‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTaca                                                                                                           <  1:2419696/289‑1 (MQ=255)
                                                                                                                                                                                       accaaggctcatcgcTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCACAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGTGGTCACt                                                                                            <  2:350130/274‑1 (MQ=255)
                                                                                                                                                                                          aagtctcatcgcTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGTGGTCACTGcc                                                                                         <  1:1605420/277‑1 (MQ=255)
                                                                                                                                                                                           agtctcatcgcTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGTGGTCACTGCCGt                                                                                       <  2:205636/279‑1 (MQ=255)
                                                                                                                                                                                            gtctcatcgcTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGTGGTCACTGCCGt                                                                                       >  1:1315723/10‑288 (MQ=255)
                                                                                                                                                                                                catcgcTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGTGGTCACTGCCGTCAGtt                                                                                  >  1:1929286/6‑289 (MQ=255)
                                                                                                                                                                                                       gAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGgtg                                                                                                                                                                                                                                                        <  2:425384/116‑1 (MQ=255)
                                                                                                                                                                                                       gAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAAcc                                                                                                                                                                                                                                             >  1:572611/1‑127 (MQ=255)
                                                                                                                                                                                                             cgcAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAata                                                                                                                                                                                                                          >  2:3185001/1‑140 (MQ=255)
                                                                                                                                                                                                             cgcAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGGTAACTACACCCATTGTGGTCACTTACGTCAGTTTATGAAtgaactg                                                                     >  2:72952/1‑289 (MQ=255)
                                                                                                                                                                                                               cAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCAt                                                                                                                         >  1:2572230/1‑235 (MQ=255)
                                                                                                                                                                                                               cAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGTGGTCACTGCCGTCAGTTTATGAAtgaactgaa                                                                   >  2:1355080/1‑289 (MQ=255)
                                                                                                                                                                                                                   ggggCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGTGGTCACTGCCGTCAGTTTATGAATGAACTGAACAg                                                                <  2:2886522/288‑1 (MQ=255)
                                                                                                                                                                                                                       cTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGaa                                                                                                                                                                              <  1:103962/174‑1 (MQ=255)
                                                                                                                                                                                                                       cTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGTGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGt                                                            <  2:1929286/288‑1 (MQ=255)
                                                                                                                                                                                                                          gACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGTGGTCACTGCCGTCAGTTTATGAATGAACTGAACAg                                                                >  2:2915497/1‑281 (MQ=255)
                                                                                                                                                                                                                            cGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGTGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGa                                                       <  1:2522203/288‑1 (MQ=255)
                                                                                                                                                                                                                               aGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGaa                                                                                                                                                                              <  1:3108707/166‑1 (MQ=255)
                                                                                                                                                                                                                               aGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGaaaa                                                                                                                                        <  2:85508/204‑1 (MQ=255)
                                                                                                                                                                                                                               aGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGTGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCt                                                    >  1:1421109/1‑288 (MQ=255)
                                                                                                                                                                                                                                  cgcgCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGTGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCg                                                 <  2:1421109/288‑1 (MQ=255)
                                                                                                                                                                                                                                    cgcTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGTGGTCACTGCCGTCAGTTTatga                                                                             >  2:3004151/1‑258 (MQ=255)
                                                                                                                                                                                                                                      cTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGc                                                                                                                               >  1:327449/1‑206 (MQ=255)
                                                                                                                                                                                                                                      cTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGc                                                                                                                               <  2:327449/206‑1 (MQ=255)
                                                                                                                                                                                                                                            ttCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGTGGTc                                                                                               <  2:1111764/232‑1 (MQ=255)
                                                                                                                                                                                                                                                           cTGGCGGCGGCCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTgg                                                                                                                                                      >  1:2341174/1‑162 (MQ=255)
                                                                                                                                                                                                                                                               cggcggcCTGTGCGCGTACGCCATTGTAGC‑ATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGAGCTTGCAGCCATCACCGTTAACTACACGCCTTGTGGTCACTGCCGTCAGTTTATGAATGAACGGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACAcgc                   >  1:3171112/1‑289 (MQ=255)
                                                                                                                                                                                                                                                               cggcggcCTGTGCGCGTACGCCATTG‑TCGAATTTTAATTTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGTGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACAcg                    <  1:72952/288‑1 (MQ=255)
                                                                                                                                                                                                                                                               cggcggcCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGTGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTTCGTATTCATCTGCCGGGCCGCGGGGCCCAcg                    >  2:3128547/1‑288 (MQ=255)
                                                                                                                                                                                                                                                               cggcggcCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGTGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACAcg                    <  1:1511154/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                ggcggcCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTgg                                                                                                                                                      >  2:211295/1‑157 (MQ=255)
                                                                                                                                                                                                                                                                 gcggcCTGTGCGCGTACGCCATTT‑GCGAATTTTAATGTTGGCGCAATGGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGTGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACAcgcg                  <  1:1194956/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                 gcggcCTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGTGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACAcgcg                  <  2:482281/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                      cTGTGCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGTGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGt            <  1:2016039/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                        gtgCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGTGGTCACTGCCGTCAGTTTatga                                                                             <  1:2257206/222‑1 (MQ=255)
                                                                                                                                                                                                                                                                        gtgCGCGTACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGTGGTCACTGCCGTCAGTTTatga                                                                             >  2:2257206/1‑222 (MQ=255)
                                                                                                                                                                                                                                                                               tACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGTGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCt    >  1:2514737/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                               tACGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCCATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACCCCTTGTGGTCACTGCCGTCAGTTTATGAATGAAATGAACAGCGGTCCGGATCCGCGTAGTCATCTGCCGGGTCGCGAGGCCAAGCCCCTGCGTGACCAtgc    >  2:1707792/1‑286 (MQ=255)
                                                                                                                                                                                                                                                                                aCGCCATTG‑TCGAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGTGGTc                                                                                               >  1:811531/1‑196 (MQ=255)
                                                                                                                                                                                                                                                                                          |||                                                                                                                                                                                                                                                                                     
ATGAGATTCAGATCACATATAAAGCCACAACGGGTTCGTAAACTGTTATCCCATTACATGATTATGAGGCAACGCCATGCATCCACGTTTTCAAACCGCTTTTGCCCAACTTGCGGATAACTTGCAATCTGCACTGGAACCTATTCTGGCAGACAAGTACTTCCCCGCTTTGTTGACCGGGGAGCAAGTCAGTAGC‑CTGAAGAGCGCAACGGGGCTGGACGAAGACGCGCTGGCATTCGCACTACTTCCGCTGGCGGCGGCCTGTGCGCGTACGCCATTG‑AGCAATTTTAATGTTGGCGCAATTGCGCGCGGTGTGAGCGGAACCTGGTATTTCGGTGCCAATATGGAATTTATTGGTGCGACAATGCAGCAAACCGTTCATGCCGAACAAAGCGCGATCAGCCACGCCTGGTTGAGTGGTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGTGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGC  >  CP042583/1806679‑1807238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A