Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A2 F210 I1 R1 4928 368.7 6392010 97.5% 6232209 231.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 1,807,379 3 bp→TCG intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0425831,807,3790AT100.0% 848.4 / NA 357intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (178/179);  total (178/179)
*CP0425831,807,3800GC99.4% 832.5 / NA 357intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base C (176/176);  total (178/179)
Fisher's exact test for biased strand distribution p-value = 4.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP0425831,807,3810CG98.3% 821.6 / NA 357intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (6/0);  new base G (172/178);  total (178/179)
Fisher's exact test for biased strand distribution p-value = 2.99e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 357 total aligned reads displayed.

GTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGTGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTAGCCACATGCCTTACAGTAAGAGCCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAGCGCGCGGTTCTGGCAGAAAAAGCCGATGCGCCGTTGATTCAGTGGGATGCCACCTCCGCAACGCTGAAAGCTCTCGGCTGTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCG  >  CP042583/1807098‑1807659
                                                                                                                                                                                                                                                                                         |||                                                                                                                                                                                                                                                                                      
           cTTGCAGCCATCACCGTTAACTACACGCCTTGTGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTcgcg                                                                                                                                                                                                                                                                         >  2:1752291/1‑288 (MQ=255)
             tGCAGCCATCACCGTTAACTACACGCCTTGTGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTg                                                                                                                                                                                                                                                                      <  1:3184313/289‑1 (MQ=255)
             ggCAGCGATCAACGTGTAGTGCACGCCTTGTGGTCACTGCGGGCCGGTTATGAATGAACTGAACAGCGGTCTGGAGCTGCGTATTCATCTGCCGGGACGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCGTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGAACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTg                                                                                                                                                                                                                                                                      <  2:2818080/288‑1 (MQ=255)
                    aTCACCGTTAACTACACGCCTTGTGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTa                                                                                                                                                                                                                                                               >  1:1010437/1‑289 (MQ=255)
                        ccGGTAACTACCAGCCTTGTGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCCGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCTCCAAGCGGTGTCGCGCTGGAATGTAAAGa                                                                                                                                                                                                                                                           <  1:853653/289‑1 (MQ=255)
                                   acGCCTTGTGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACCTGCCTTACAGTAAGTCGCCAATCTGGGTCGCGCTGGAATTTAAAGGAGGTCGTAttt                                                                                                                                                                                                                                                >  2:2594394/1‑289 (MQ=255)
                                   acGCCTTGTGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGa                                                                                                                                                                                                                                                                    <  1:304553/269‑1 (MQ=255)
                                     gCCTTGTGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGt                                                                                                                                                                                                                                                       >  1:430686/1‑280 (MQ=255)
                                       cTTGTGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTg                                                                                                                                                                                                                                                                      >  1:377793/1‑263 (MQ=255)
                                       cTTGTGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTg                                                                                                                                                                                                                                                                      <  2:377793/263‑1 (MQ=255)
                                          gtgGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGGGGTGGCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGtt                                                                                                                                                                                                                                          >  2:775003/1‑287 (MQ=255)
                                                 cTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTTATGCGCTTTCGCAGGCAGCGATTGCGGCGGCAAACCGCTCGCACAAGCCTTACAGTAAGTCGCCAAGCGGATGCGCGCTGGAATGTAAAGACGGGCGGATTTTCCGCGGCAGcgc                                                                                                                                                                                                                                   >  2:220334/1‑286 (MQ=255)
                                                     cGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTg                                                                                                                                                                                                                              >  1:1317950/1‑289 (MQ=255)
                                                          gTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGa                                                                                                                                                                                                                                                           >  2:90936/1‑255 (MQ=255)
                                                          gTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGa                                                                                                                                                                                                                                                           <  1:90936/255‑1 (MQ=255)
                                                            ttATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAAcgc                                                                                                                                                                                                                       <  1:1937082/289‑1 (MQ=255)
                                                              atgaatgaACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTCAAACGCAGCTGATGGCCGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCTGCGGCCAACCGTCCGCACATGCCTTAAAGCAAGTCGCCAAGCGGTGTCGCGCTGGAAGGTCAAGACTGCCGTATTTTCAGTGGCAGGTACCCGGAAAAcgccg                                                                                                                                                                                                                     >  2:497411/1‑289 (MQ=255)
                                                              atgaatgaACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAAcgccg                                                                                                                                                                                                                     <  1:1372201/289‑1 (MQ=255)
                                                               tgaatgaaCTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGgtg                                                                                                                                                                                                                                                                              <  2:1972071/231‑1 (MQ=255)
                                                                     aactgaacAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCa                                                                                                                                                                                                               >  2:970328/1‑288 (MQ=255)
                                                                      cctgaaaAGCGGTCTGGACCGGCGTCTGCAGCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGTGCTGGAGATTAAAACGCTGCGGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCAAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAAc                                                                                                                                                                                                             <  2:2871853/288‑1 (MQ=255)
                                                                        tgaacAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAAccc                                                                                                                                                                                                           <  1:673651/289‑1 (MQ=255)
                                                                               cGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGActct                                                                                                                                                                                                     >  1:776545/1‑288 (MQ=255)
                                                                               cGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGActct                                                                                                                                                                                                     >  1:1146715/1‑288 (MQ=255)
                                                                                ggTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGc                                                                                                                                                                                                   <  1:2638096/289‑1 (MQ=255)
                                                                                  tCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGcc                                                                                                                                                                                                  >  1:2117408/1‑288 (MQ=255)
                                                                                   gtgcagcttggTATTCATCTGACGGGCCGGGAGGCGCACGCGCTGCGTGACTATCTGCCAGCTGCGGTGAGGCCGAAGGATCTGGAAATTAAAACGCTGCTGATGGAGGAACAGGATCACGGCTATGCGCTGACGGGTGATGTGCTTTCTCAGGCAGCGATGGCGGCGGCAAACCGTTCGCACATGCCTTGCAGTAAGTTGCCAAGCGGTTTCGCGCTGGAATGTTAAGACGGTCGTATTTTCAGTTGCAGCTACGGTGAAAACGCCGCATTCAACCCGACTCTGCCa                                                                                                                                                                                                 <  2:670004/278‑1 (MQ=255)
                                                                                   cTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCa                                                                                                                                                                                                 <  1:1539973/288‑1 (MQ=255)
                                                                                    tGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCAcc                                                                                                                                                                                               >  1:2372963/1‑289 (MQ=255)
                                                                                           gCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTCTTTTCAGTGGCAGCCACGGCGAAGACGCCGCATTCAACCCGAACCTGCCACCGTTGccg                                                                                                                                                                                        >  2:594037/1‑287 (MQ=255)
                                                                                            cGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCAcc                                                                                                                                                                                               >  2:3099383/1‑281 (MQ=255)
                                                                                            cGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCGGAAAACGCCGCATTCAACCCGACCATGCCCCCGTTTCatg                                                                                                                                                                                       >  2:933000/1‑287 (MQ=255)
                                                                                             gTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAggg                                                                                                                                                                                      <  1:1853795/289‑1 (MQ=255)
                                                                                             gTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAggg                                                                                                                                                                                      <  1:755053/289‑1 (MQ=255)
                                                                                                      tGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCa                                                                                                                                                                                                 <  1:2818604/269‑1 (MQ=255)
                                                                                                      tGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCa                                                                                                                                                                                                 <  2:1625826/269‑1 (MQ=255)
                                                                                                        ccGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTg                                                                                                                                                                                                                                                                      <  2:11292/198‑1 (MQ=255)
                                                                                                            gCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCa                                                                                                                                                                                                 <  1:2013253/263‑1 (MQ=255)
                                                                                                             ccGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCt                                                                                                                                                                                                                                    <  2:1828988/227‑1 (MQ=255)
                                                                                                              ctcgAGGCACCCGCGTTGAGGGACTATCTGCAAGATTCCTTTGGGCCACAAGATCTGGAGATTAAAACGCTGCTGCTGGACGAACAGACTCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGa                                                                                                                                                                     <  2:1294996/287‑1 (MQ=255)
                                                                                                              cgcgAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGa                                                                                                                                                                     <  2:1156183/289‑1 (MQ=255)
                                                                                                                  aGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCCGTTg                                                                                                                                                                      >  2:35341/1‑284 (MQ=255)
                                                                                                                     cacaCGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGCTCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCa                                                                                                                                                               <  1:2060176/288‑1 (MQ=255)
                                                                                                                     cacaCGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAAtctc                                                                                                                                                                <  1:2227042/287‑1 (MQ=255)
                                                                                                                             cTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTcgcg                                                                                                                                                                                                                                                                         >  2:2076293/1‑174 (MQ=255)
                                                                                                                             cTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTACTTCTGTTGAATCTCAAGGGTTATg                                                                                                                                                      >  1:2315434/1‑289 (MQ=255)
                                                                                                                               gCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGAt                                                                                                                                                    >  1:257639/1‑289 (MQ=255)
                                                                                                                                  tGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGCTGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTa                                                                                                                                                  <  2:2297367/288‑1 (MQ=255)
                                                                                                                                  tGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGACTTCAACCCGACTCTGCCACCGTTGCAGGGACCGTTAATTCTGTTGAATCTCCAgctgctccttta                                                                                                                                                  >  2:1571295/1‑277 (MQ=255)
                                                                                                                                  gcgttataTGCCACAGGCCTTAGGGCCGGAAGATGTGGAGCTTACAACGCAGCTAATGGACGAACAGAATCACGGCTATGCGCTGCCGGGTGATGCGCTTCCTCGGGCAGCGATGGCGGCGGCAAACCGTTAGCACATGCCTTACAGTAATTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGTGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTa                                                                                                                                                  <  2:1203480/284‑1 (MQ=255)
                                                                                                                                   gACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTAcc                                                                                                                                                <  1:3073745/289‑1 (MQ=255)
                                                                                                                                   gACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTAcc                                                                                                                                                <  2:1010437/289‑1 (MQ=255)
                                                                                                                                        tCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTAc                                                                                                                                                                                                                                  <  2:2361846/202‑1 (MQ=255)
                                                                                                                                        tCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTCCAGGGAGCGTTAATTTTGTTGAAACTCAAGGGTTATGATTACCCGGat                                                                                                                                           >  2:3073745/1‑289 (MQ=255)
                                                                                                                                          tGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGata                                                                                                                                          >  1:2852211/1‑288 (MQ=255)
                                                                                                                                            ccAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGa                                                                                                                                                                                                         >  1:2423912/1‑223 (MQ=255)
                                                                                                                                             cAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCa                                                                                                                                      <  1:2479077/289‑1 (MQ=255)
                                                                                                                                               gATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGAGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAgc                                                                                                                                    >  2:1743524/1‑289 (MQ=255)
                                                                                                                                                  gCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAgcgc                                                                                                                                  >  2:2417395/1‑288 (MQ=255)
                                                                                                                                                   ccTTTGGGCCGAAAGATCGTGGGCTTAAAACGCTGCGGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATTCGATTTCTCAGGCAGCGATTGCGGCGGCAAACCGTGCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTTGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAgcgcg                                                                                                                                 <  2:2676259/288‑1 (MQ=255)
                                                                                                                                                    cTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAgcgcgc                                                                                                                                <  1:2885582/288‑1 (MQ=255)
                                                                                                                                                              aaaGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTTTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAGCGCGCGGTTCTGGCAg                                                                                                                     >  1:2740359/1‑289 (MQ=255)
                                                                                                                                                              aaaGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAggg                                                                                                                                                                                      <  1:1266546/224‑1 (MQ=255)
                                                                                                                                                                   tCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAGCGCGCGGTTCTGGCAGaaaaa                                                                                                                <  1:1451631/289‑1 (MQ=255)
                                                                                                                                                                           ttAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTTAATCTCAAGGGTTATGATTACCCGGATATCCAGCGCGCGGTTCTGGCAGAAAAAGCCGATg                                                                                                         >  2:2992835/1‑288 (MQ=255)
                                                                                                                                                                            tAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTcgcg                                                                                                                                                                                                                                                                         >  1:581311/1‑127 (MQ=255)
                                                                                                                                                                            tAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCa                                                                                                                                                               <  1:365850/233‑1 (MQ=255)
                                                                                                                                                                             aaaaCGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAGCGCGCGGTTCTGGCAGAAAAAGCCGATgcgc                                                                                                      <  1:22690/289‑1 (MQ=255)
                                                                                                                                                                                    tgctgATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAAtctc                                                                                                                                                                <  2:105404/224‑1 (MQ=255)
                                                                                                                                                                                      ctgATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAgg                                                                                                                                                                                       <  1:2956484/199‑1 (MQ=255)
                                                                                                                                                                                              cGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCGACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAGCGCGCGGTTCTGGCAGAAAAAGCCGATGCGcc                                                                                                     <  2:2018500/273‑1 (MQ=255)
                                                                                                                                                                                                aaCAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGa                                                                                                                                                                                                                                                           >  1:536055/1‑121 (MQ=255)
                                                                                                                                                                                                aaCAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCCACCCTGAAAACGCCGCATTCAACCCGCCTCTGCCACCGTTGCAGGGACCGTTACTTCTGTTGAATCCCAAGGGTCATGATTACCCGGCTACCCAGCGCGCGGTTCTCGCCGAAACACCCCATCCGCCGTCGACTCACTGCGacc                                                                                    >  2:1427024/1‑286 (MQ=255)
                                                                                                                                                                                                        cACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAGCGCGCGGTTCTGGCAGAAAAAGCCGATGCGCCGTTGATTCAGTGGGATGCCACCTCCg                                                                           <  1:2541869/289‑1 (MQ=255)
                                                                                                                                                                                                          cGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAGCGCGCGGTTCTGGCAGAAAAAGCCGATGCGCCGTTGATTCAGTGGGATGCCACCTCCGCa                                                                         <  2:2538835/289‑1 (MQ=255)
                                                                                                                                                                                                             cTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAGCGCGCGGTTCTGGCAGAAAAAGCCGATGCGCCGTTGATTCa                                                                                            >  2:2248228/1‑267 (MQ=255)
                                                                                                                                                                                                                tGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTg                                                                                                                                                                      >  2:1506644/1‑190 (MQ=255)
                                                                                                                                                                                                                tGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCa                                                                                                                                                               <  1:2179892/197‑1 (MQ=255)
                                                                                                                                                                                                                tGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTa                                                                                                                                                  <  2:2954147/210‑1 (MQ=255)
                                                                                                                                                                                                                tGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAGCGCGCGGTTCTGGCAGAAAAAGCCGATGCGCCGTTGATTCAGTGGGATGCCCACCCCGCAACCCtt                                                                   >  2:2162140/1‑288 (MQ=255)
                                                                                                                                                                                                                 gcgcTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAGGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGTGTTATGATTACCCGGATATCCAGCGCGCGGTTCTGGCAGAAAAAGCCGATGCGCCGTTGATTCAGTGGGATGCCACCTCCGCAACGCTGa                                                                  <  1:497411/289‑1 (MQ=255)
                                                                                                                                                                                                                  cgcTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAGCGCGCGGTTCTGGCAGAAAAAGCCGATGCGCCGTTGATTCAGt                                                                                          <  1:1686317/264‑1 (MQ=255)
                                                                                                                                                                                                                     tGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAGCGCGCGGTTCTGGCAGAAAAAGCCGATGCGCCGTTGATTCAGTGGGATGCCACCTCCGCAACGCTGAAAg                                                               >  2:1888706/1‑288 (MQ=255)
                                                                                                                                                                                                                       aCGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTgg                                                                                                                                                                                                                                                                     >  1:938231/1‑88 (MQ=255)
                                                                                                                                                                                                                       aCGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAAc                                                                                                                                                                                                             >  1:1810258/1‑144 (MQ=255)
                                                                                                                                                                                                                          ggTGCTGCGCTTGGTGGGGGAGCGATTGCGCGGGCAAACCGGGCGCACATGCCTGACAGTAAGTCGCCAGGCGGGGTCGCGCTGGAATGTAAAGACGGTCGTATTTGCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCAAACGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAGCGCGCGGTTCTGGCAGAAAAAGCCGATGCGCCGTTGATTCAGTGGGATGCCACCTCCGCAACGCTGAAAGCtctc                                                          <  2:323085/288‑1 (MQ=255)
                                                                                                                                                                                                                             gATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAGCGCGCGGTTCTGGCAGAAAAAGCCGATGCGCCGTTGATTCAGTGGGATGCCACCTCCGCAACGCTGAAAGCTCTCGGc                                                       <  1:1888706/288‑1 (MQ=255)
                                                                                                                                                                                                                                 cgcTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCaa                                                                                                                                                              <  1:1614106/181‑1 (MQ=255)
                                                                                                                                                                                                                                 cgcTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAGCGCGCGGTTCTGGCAGaaaa                                                                                                                 >  2:832281/1‑226 (MQ=255)
                                                                                                                                                                                                                                 cgcTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAGCGCGCGGTTCTGGCAGAAAAAGCCGATGCGCCGTTGATTCAGTGGGATGc                                                                                   >  2:740294/1‑256 (MQ=255)
                                                                                                                                                                                                                                 cgcTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAGCGCGCGGTTCTGGCAGAAAAAGCCGATGCGCCGTTGATTCAGTGGGATGCCACCTCCGCAACGCTGAAAGCCCTCGGCTGTc                                                   >  1:1095480/1‑288 (MQ=255)
                                                                                                                                                                                                                                  gcTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAGCGCGCGGTTCTGGCAGAAAAAGCCGATGCGCCGTTGATTCAGTGGGATGCCACCTCCGCAACGCTGAAAGCTCTCGGCTGTca                                                  <  1:600935/288‑1 (MQ=255)
                                                                                                                                                                                                                                      tctcAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAGCGCGCGGTTCTGGCAGAAAAAGCCGATGCGCCGTTGATTCAGTgg                                                                                        >  2:556488/1‑246 (MQ=255)
                                                                                                                                                                                                                                         cAGGCAGCGATTGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAGCGCGCGGTTCTGGCAGAAAAAGCCGATGCGCCGTTGATTCAGTGGGATGCCACCTCCGCAACGCTGAAAGCTCTCGGc                                                       <  1:3093640/276‑1 (MQ=255)
                                                                                                                                                                                                                                                    tGCGGCGGCAAACCGTTCGCACATGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAGCGCGCGGTTCTGGCAGAAAAAGCCGATGCGCCGTTGATTCAGTGGGATGCCACCTCCGCAACGCTGAAAGCTCTCGGCTGTCACAGTATCGACCGAGTGCtt                               <  1:731737/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                      gcacaTGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAGCGCGCGGTTCTGGCAGAAAAAGCCGATGCGCCGTTGATTCAGTGGGATGCCACCTCCGCAACGCTGAAAGCTCTCGGCTGTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGc             <  1:1743524/288‑1 (MQ=255)
                                                                                                                                                                                                                                                                           tGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAgcgcg                                                                                                                                 >  2:405327/1‑168 (MQ=255)
                                                                                                                                                                                                                                                                           tGCCTTACAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAGCGCGCGGTTCTGGCAGAAAAAGCCGATGCGCCGTTGATTCAGTGGGATGCCACCTCCGCAACGCTGAAAGCTCTCGGCTGTCACAGTATCGACCGTGTGCTTCCCGCTTAAGCCTGGTGCCgggt        >  2:1346550/1‑287 (MQ=255)
                                                                                                                                                                                                                                                                                  cAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTa                                                                                                                                                  >  2:3061333/1‑144 (MQ=255)
                                                                                                                                                                                                                                                                                  cAGTAAGTCGCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAGCGCGCGGTTCTGGCAGAAAAAGCCGATGCGCCGTTGATTCAGTGGGATGCCACCTCCGCAACGCTGAAAGCTCTCGGCTGTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATgcgg    >  2:2475623/1‑286 (MQ=255)
                                                                                                                                                                                                                                                                                         |||                                                                                                                                                                                                                                                                                      
GTGAAAAAGCGCTTGCAGCCATCACCGTTAACTACACGCCTTGTGGTCACTGCCGTCAGTTTATGAATGAACTGAACAGCGGTCTGGATCTGCGTATTCATCTGCCGGGCCGCGAGGCACACGCGCTGCGTGACTATCTGCCAGATGCCTTTGGGCCGAAAGATCTGGAGATTAAAACGCTGCTGATGGACGAACAGGATCACGGCTATGCGCTGACGGGTGATGCGCTTTCTCAGGCAGCGATTGCGGCGGCAAACCGTAGCCACATGCCTTACAGTAAGAGCCCAAGCGGTGTCGCGCTGGAATGTAAAGACGGTCGTATTTTCAGTGGCAGCTACGCTGAAAACGCCGCATTCAACCCGACTCTGCCACCGTTGCAGGGAGCGTTAATTCTGTTGAATCTCAAGGGTTATGATTACCCGGATATCCAGCGCGCGGTTCTGGCAGAAAAAGCCGATGCGCCGTTGATTCAGTGGGATGCCACCTCCGCAACGCTGAAAGCTCTCGGCTGTCACAGTATCGACCGAGTGCTTCTCGCTTAAGCCTGGTGCCGGATGCGGCG  >  CP042583/1807098‑1807659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A