Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F210 I1 R1	4928	368.7	6392010	97.5%	6232209	231.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP042583	1,949,345	3 bp→CGA	intergenic (–/–)	– / –	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP042583	1,949,345	0	G	C	98.9%	615.8 / NA	268	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base G (1/2); new base C (131/131); total (133/135)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP042583	1,949,346	0	C	G	98.1%	607.6 / NA	268	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base C (2/3); new base G (131/131); total (133/135)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*	CP042583	1,949,347	0	T	A	99.2%	629.0 / ‑5.4	268	intergenic (–/–)	–/–	–/–
Reads supporting (aligned to +/- strand): ref base T (1/0); major base A (132/133); minor base G (0/2); total (133/135)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 268 total aligned reads displayed.

GTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGGCTGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTGCTAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGGCTAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATG‑CAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATC  >  CP042583/1949066‑1949627

|||

gTTTAGCGGAATAAGATAGAAACGCTCACCCTCGCTTTGCGGCGTGGGCAAAAGCGGGAAAATTTGCCCGCCGTTGTGGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCa                                                                                                                                                                                                                                                                                      <  1:1065276/289‑1 (MQ=255)

   ttGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAACGCGCatca                                                                                                                                                                                                                                                                                   >  2:454743/1‑289 (MQ=255)

       ggCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCatcaatc                                                                                                                                                                                                                                                                                <  1:2583879/288‑1 (MQ=255)

        gCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGTGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCatcaatca                                                                                                                                                                                                                                                                               <  1:1193272/288‑1 (MQ=255)

           tCAGATAGAAACGCTCACGTTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACg                                                                                                                                                                                                                                                                           <  1:3107104/289‑1 (MQ=255)

             aGATAGAAACGCTCCCGGTCGCTTTGCGGGGTTGGCAACCGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTa                                                                                                                                                                                                                                                                         <  2:3132189/289‑1 (MQ=255)

                        ctcacgcgcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCTGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTTGCCCCACGGTTGCTGGACACCGGGTAACCTGCCGGAAGTTGCCAAATCGCCTCACTCAGACTTACCACCAGGCta                                                                                                                                                                                                                                                              >  2:2484480/1‑288 (MQ=255)

                         tcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGt                                                                                                                                                                                                                                                             >  2:1609412/1‑289 (MQ=255)

                          cacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCatcaatca                                                                                                                                                                                                                                                                               <  1:2509377/270‑1 (MQ=255)

                                           gTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGcc                                                                                                                                                                                                                                           <  2:2154431/289‑1 (MQ=255)

                                            ttGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGcc                                                                                                                                                                                                                                           >  2:128755/1‑288 (MQ=255)

                                                caacagcgagAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTg                                                                                                                                                                                                                                       <  1:1462901/279‑1 (MQ=255)

                                                      cgagAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGc                                                                                                                                                                                                                                >  2:823208/4‑289 (MQ=255)

                                                         gAAAATGTGCCCGCCGTTGTTCTTCACCACAAGTAATACCAGCGGCGCGGAAACCTGACGCAATAACGCAAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAg                                                                                                                                                                                                                              <  2:2066102/288‑1 (MQ=255)

                                                                                 caccacAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACg                                                                                                                                                                                                                                                                           >  1:1099260/1‑219 (MQ=255)

                                                                                   ccacAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCCC‑ATAAGCGATGCCCCAGtt                                                                                                                                                                                                   >  2:1389729/1‑288 (MQ=255)

                                                                                    cacaATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTgc                                                                                                                                                                                                 >  1:1898792/1‑289 (MQ=255)

                                                                                              aCCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCaa                                                                                                                                                                                       >  2:269617/1‑289 (MQ=255)

                                                                                                   cggcgCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGACGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCCGTTCATGCAGATAGTCGC‑AGAGGCGATGCGCCAGTTGCCCTTCGCCAAACGCa                                                                                                                                                                                  >  2:535777/1‑289 (MQ=255)

                                                                                                            aaccacaccgtaTAACGCCGGCGCTTTGAGGTCGTAAAGTGCGGAGAGCTCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCACGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCAcgg                                                                                                                                                                            <  2:3030074/275‑1 (MQ=255)

                                                                                                            aaCCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCAcggcg                                                                                                                                                                          <  2:321136/288‑1 (MQ=255)

                                                                                                                 gACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAAt                                                                                                                                                                    >  2:1090064/1‑289 (MQ=255)

                                                                                                                     cAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAAcc                                                                                                                                                                >  1:2778345/1‑289 (MQ=255)

                                                                                                                       aTAACGCTAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTaccac                                                                                                                                                                                                                                                                     <  2:2034891/187‑1 (MQ=255)

                                                                                                                       aTAACGCTAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTaccac                                                                                                                                                                                                                                                                     >  1:2034891/1‑187 (MQ=255)

                                                                                                                        tAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGcc                                                                                                                                                             <  1:2527866/289‑1 (MQ=255)

                                                                                                                        tAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGc                                                                                                                                                              <  2:95731/288‑1 (MQ=255)

                                                                                                                             ccAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCAt                                                                                                                                                        >  1:1102050/1‑289 (MQ=255)

                                                                                                                                gcgcGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACcaacaa                                                                                                                                                                                                                                                    <  2:2864071/195‑1 (MQ=255)

                                                                                                                                gcgcGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACcaacaa                                                                                                                                                                                                                                                    >  1:2864071/1‑195 (MQ=255)

                                                                                                                                gcgcGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTg                                                                                                                                                      >  1:1479638/1‑288 (MQ=255)

                                                                                                                                     ttGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCCGCCGGAAGTTGCGAAAGCGCatcaatc                                                                                                                                                                                                                                                                                <  2:1595187/162‑1 (MQ=255)

                                                                                                                                     ttGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCCGCCGGAAGTTGCGAAAGCGCatcaatc                                                                                                                                                                                                                                                                                >  1:1595187/1‑162 (MQ=255)

                                                                                                                                      tGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCACCACGGCGGGCAATAACCGCCTGCATTGCCTG‑tt                                                                                                                                               >  1:629638/1‑288 (MQ=255)

                                                                                                                                        agaTCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCaaa                                                                                                                                                                                      <  1:1732713/248‑1 (MQ=255)

                                                                                                                                          aTCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGcc                                                                                                                                                                                         >  1:96864/1‑243 (MQ=255)

                                                                                                                                                     cGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑Aga                                                                                                                                                                                                                  >  2:2667250/1‑207 (MQ=255)

                                                                                                                                                     cGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑Aga                                                                                                                                                                                                                  <  1:2667250/207‑1 (MQ=255)

                                                                                                                                                     cGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCAcgg                                                                                                                                                                            >  1:1086852/1‑245 (MQ=255)

                                                                                                                                                     cGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGgcgc                                                                                                                                    >  1:2562706/1‑284 (MQ=255)

                                                                                                                                                     cGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGgcgc                                                                                                                                    <  2:2562706/284‑1 (MQ=255)

                                                                                                                                                     cGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACAGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGAt                                                                                                                               >  1:239792/1‑289 (MQ=255)

                                                                                                                                                           gaTCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCaa                                                                                                                          <  2:257977/288‑1 (MQ=255)

                                                                                                                                                           gaTCGCCCACAATCGCCAGCGTCGGGTTGCGGGTTGCCCGCTGAAGGCCGGCGGCGGTCGAAAGCAGCGCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCaa                                                                                                                          <  2:2520636/288‑1 (MQ=255)

                                                                                                                                                            aTCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCCCCAGTTGCGCTTCGCCAACAGCAACACGGGGGGGAATAACCGCCTGCATTGCCCG‑TTCCGCCAGGTGCGGGGATTCAAc                                                                                                                         >  1:332203/1‑288 (MQ=255)

                                                                                                                                                             tCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGc                                                                                                                       <  2:1773638/289‑1 (MQ=255)

                                                                                                                                                                    caATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGc                                                                                                                                                              <  2:558105/244‑1 (MQ=255)

                                                                                                                                                                    caATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGc                                                                                                                                                              >  1:558105/1‑244 (MQ=255)

                                                                                                                                                                    caATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAgg                                                                                                                 >  1:2374220/1‑288 (MQ=255)

                                                                                                                                                                      aTCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTaccacc                                                                                                                                                                                                                                                                    >  1:410057/1‑141 (MQ=255)

                                                                                                                                                                          ccAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCg                                                                                                          >  1:2904861/1‑289 (MQ=255)

                                                                                                                                                                           cAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCCCTTCGCCAAACGCAACACGGCGGGCAATAACCGCCTGCATTCCCTT‑GTTCGCCCGGCGCGGGATTTCCACCCACCCAGGCCGGccg                                                                                                         >  2:2378436/1‑287 (MQ=255)

                                                                                                                                                                               gTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGcc                                                                                                                                                                                                                                           <  1:1164973/157‑1 (MQ=255)

                                                                                                                                                                                 cGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTgcg                                                                                                                                                                                                <  1:1254434/197‑1 (MQ=255)

                                                                                                                                                                                 cGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTgcg                                                                                                                                                                                                >  2:1254434/1‑197 (MQ=255)

                                                                                                                                                                                   gTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACAAACAAAAAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGgcgc                                                                                                                                    <  1:3044629/254‑1 (MQ=255)

                                                                                                                                                                                     gtGCCGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGcc                                                                                                <  1:152992/287‑1 (MQ=255)

                                                                                                                                                                                        ccGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGAt                                                                                            >  1:2226385/1‑289 (MQ=255)

                                                                                                                                                                                         cgtgtGCCCGCTGAACGCCGGCGGCGGTCGAAAGCATCCCGTCGATACCGCTGGCCCCACTGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATg                                                                                           <  2:1479638/285‑1 (MQ=255)

                                                                                                                                                                                         cGCTTGCCCGCTGAACGCCGGCGGCGGTCGAAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATg                                                                                           <  1:2004936/289‑1 (MQ=255)

                                                                                                                                                                                                                    tcgaaaGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATG‑CAGCTCCAGCCAGTCGGCAATATTgg                                                                <  1:3029799/284‑1 (MQ=255)

                                                                                                                                                                                                                    tcgaaaGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATG‑CAGCTCCAGCCAGTCGGCAATATTgg                                                                <  2:1898792/284‑1 (MQ=255)

                                                                                                                                                                                                                           cagcCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATG‑CAGCTCCAGCCAGTCGGCAATATTGGCAATTaa                                                         >  1:141009/1‑288 (MQ=255)

                                                                                                                                                                                                                              cccGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCAcggcg                                                                                                                                                                          >  1:2615654/1‑174 (MQ=255)

                                                                                                                                                                                                                              cccGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCAcggcg                                                                                                                                                                          <  2:2615654/174‑1 (MQ=255)

                                                                                                                                                                                                                              cccGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAAt                                                                                                                                                                    <  1:849957/180‑1 (MQ=255)

                                                                                                                                                                                                                              cccGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAAt                                                                                                                                                                    >  2:849957/1‑180 (MQ=255)

                                                                                                                                                                                                                                cGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATG‑CAGCTCCAGCCAGTCGGCaa                                                                      >  1:3195008/1‑270 (MQ=255)

                                                                                                                                                                                                                                 gTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATG‑CAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGt                                                  <  1:2484480/289‑1 (MQ=255)

                                                                                                                                                                                                                                  tCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCAcgg                                                                                                                                                                            >  2:1178854/1‑168 (MQ=255)

                                                                                                                                                                                                                                  tCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑t                                                                                                                                                <  1:1630189/195‑1 (MQ=255)

                                                                                                                                                                                                                                  tCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACCCACCAGGGCTGGCGTTTTTCTGCCGGATG‑CAGGCCCCGCCAGTCGGCCATATTGGCAATTAAGCGAccg                                                  >  2:2961230/1‑286 (MQ=255)

                                                                                                                                                                                                                                     aTACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGTGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATG‑CAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACCTCcgc                                              >  1:127670/1‑289 (MQ=255)

                                                                                                                                                                                                                                      tACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATG‑CAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCcgc                                              >  2:462712/1‑288 (MQ=255)

                                                                                                                                                                                                                                         cGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATG‑CAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATg                                          <  1:269617/289‑1 (MQ=255)

                                                                                                                                                                                                                                         cGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATG‑CAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATg                                          <  1:941634/289‑1 (MQ=255)

                                                                                                                                                                                                                                         cGCTGGCCCCACGGTTGATGTACACCGTGTAACCTGCCGGAAGTTGAGAAAGCGCATCAATGAGACGTACCACCAGGCTGTTACCAACAAACAAGTGCCCCTGTGCAGGCAGATAGTCGC‑AGAGGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGACTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATG‑CAGCTCCAGCCAGTCGGCAATATGGGCAATTAAGCGACGTCCGCGATg                                          <  1:322642/289‑1 (MQ=255)

                                                                                                                                                                                                                                           cTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCCCCAGGGCTGGCGTTTTTCTTCCGGATG‑CAGCTCCAGCCAGTCGGCAATATTGGCAATTAAACGACGTCCGCGAgggg                                        >  2:1939136/1‑288 (MQ=255)

                                                                                                                                                                                                                                              gCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATG‑CAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGgtgt                                      >  1:1003574/1‑288 (MQ=255)

                                                                                                                                                                                                                                              gCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATG‑CAGCCCCAGCCAGTCGGCAAGCTTGGCAATTAAGCGAGCTCCGCGATGgtgt                                      >  2:1456188/1‑288 (MQ=255)

                                                                                                                                                                                                                                               ccccccGGTTCCTGTACACCGGGTAACCTGCCGAAAGTTGCAAAAGCGCATCAAGCAGCCGTACCCCCATGATGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATG‑CAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGtgtg                                     <  2:332231/288‑1 (MQ=255)

                                                                                                                                                                                                                                               ccccACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCCCGGGATTTCAACGCACCCAGGCTGGCGGTTTTCTTCCGGTGC‑CAGCCCCTGCCAGTCGGCACTCTTGTCAAATACACAACGGCcgcggaagctgg                                     >  2:2942136/1‑280 (MQ=255)

                                                                                                                                                                                                                                                         gCTGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATG‑CAGCTCCAGCCAGTCGGCAATATTgg                                                                <  1:1311109/251‑1 (MQ=255)

                                                                                                                                                                                                                                                           tGTACACCGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATG‑CAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCg                        <  2:697628/289‑1 (MQ=255)

                                                                                                                                                                                                                                                                 ccGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATG‑CAGCTCCAGCCAGTCGGCAATATTGGCAATTAAgag                                                      <  1:2114018/253‑3 (MQ=255)

                                                                                                                                                                                                                                                                 ccGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATG‑CAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCg                                                      <  1:819787/253‑1 (MQ=255)

                                                                                                                                                                                                                                                                  cGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATG‑CAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCa                 >  1:1787230/1‑289 (MQ=255)

                                                                                                                                                                                                                                                                  cGGGTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATG‑CAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCa                 <  1:536270/289‑1 (MQ=255)

                                                                                                                                                                                                                                                                    ggTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGTCGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATG‑CAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGt                          >  1:2308891/1‑278 (MQ=255)

                                                                                                                                                                                                                                                                    ggTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGTCGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATG‑CAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGt                          <  2:2308891/278‑1 (MQ=255)

                                                                                                                                                                                                                                                                    ggTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATG‑CAGCTCCAGCCCGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGCGCCAGTCGCCCTTCAAt               >  2:978612/1‑289 (MQ=255)

                                                                                                                                                                                                                                                                    ggTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATG‑CAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAAt               >  1:1862030/1‑289 (MQ=255)

                                                                                                                                                                                                                                                                    ggTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATG‑CAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGGCCGCGAGTGTGTGCCGGAGCAAg                           >  1:705986/1‑277 (MQ=255)

                                                                                                                                                                                                                                                                     gTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAg                                                                                                                                                                                                     <  2:191503/108‑1 (MQ=255)

                                                                                                                                                                                                                                                                     gTAACCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAg                                                                                                                                                                                                     >  1:191503/1‑108 (MQ=255)

                                                                                                                                                                                                                                                                       aaCCTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCTTGTCAAGGAAGA‑AGTCGCAAGTTGCGATCCCCCGGTT‑CCCTTCCCCAAAAGGAACCCCGCCGGGAATTAACCCCCGCATTTCCTGTTTCCCCCGGGCCGGGGATTTAAAC‑CACCCAGGCCGGGGTTTTTCTTCCGGGTGCCGGCCCAAGCCGTCCGCCAATATGGGAATTTAACGAACGTCGGCG‑TGGTGTGGCGGGTCAAAGCGGCCTTCaaatgta           >  2:1081697/1‑284 (MQ=255)

                                                                                                                                                                                                                                                                         ccTGCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATG‑CAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGtcat          <  1:294568/289‑1 (MQ=255)

                                                                                                                                                                                                                                                                            gCCGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATG‑CAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGAc                                                    >  1:467689/1‑244 (MQ=255)

                                                                                                                                                                                                                                                                             ccGGAAGTTGCGAAAACGCATCAATCAGACTGACCACCAGGCTGTTACCAACAAACAATTGCCCCGGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATG‑CAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATcaa       <  2:1102050/288‑1 (MQ=255)

                                                                                                                                                                                                                                                                              cGGAAGTTGCGAAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATG‑CGGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGGTCAAGTCGCCCTTCCATGCCATccaca     >  2:548581/1‑289 (MQ=255)

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GTTTTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGGCTGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTTGCCGCTTGCCCGCTGAACGCCGGCGGCGGTGCTAAGCAGCCCGTCGATACCGCTGGCCCCACGGTTGCTGTACACCGGGTAACCTGCCGGAAGTTGGCTAAGCGCATCAATCAGACGTACCACCAGGCTGTTACCAACAAACAATTGCCCCTGTTCAGGCAGATAGTCGC‑AGATGCGATGCGCCAGTTGCGCTTCGCCAAACGCATCACGGCGGGCAATAACCGCCTGCATTGCCTG‑TTCCGCCAGGCGCGGGATTTCAACGCACCAGGGCTGGCGTTTTTCTGCCGGATG‑CAGCTCCAGCCAGTCGGCAATATTGGCAATTAAGCGACGTCCGCGATGGTGTGCCGGATCAAGTCGCCCTTCAATGTCATCAACAATC  >  CP042583/1949066‑1949627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A