Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A3 F210 I1 R1 4927 250.2 4002508 96.7% 3870425 266.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 833,892 3 bp→TCA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583833,8920AT99.5% 475.8 / NA 201intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (92/108);  minor base G (1/0);  total (93/108)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583833,8930GC99.5% 475.3 / NA 201intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (92/108);  minor base A (1/0);  total (93/108)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583833,8940TA99.5% 472.0 / NA 201intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base A (92/108);  minor base C (1/0);  total (93/108)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 201 total aligned reads displayed.

GGAAAACTTTGGTATAACCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGATGGCATTCCAGTGTTGCTGGAAACGGAAGCCCGCGTGCTGACTGCTGATGAGAGTAAAAGTTGATGTAAACTGCTGATGAGAGTAAATCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAAAGTGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGAGTGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGAACCGATGATCCCTGCGACAATCATTCGTCAGGTTGCTGATAACCTCGCTCAGCGTCAGGTGGGTATGGCGACTCTGGCGGTGCCAATCCACAATGCG  >  CP042583/833612‑834173
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                       
ttgaaatcattgcctgcccggttcgcaacggaaaactttggtataaccaggaaaaacaagagcttatttgcaaactggacaacctcgctttcccgctgcgtgatggcattccagtgttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGcc                                                                                                                                                                                                                                                                                   <  2:14205‑M2/128‑1 (MQ=255)
ttgaaatcattgcctgccaggtttgcaacggaaaactttggtataaccaggaaaaacatgagcttatttgcaaactgggcaacctcgctttcccgctgcgtgatggcattccagtgttgctggaaacggaagcccgcgtgctgactgcggatgggagtaaaTCATGGGTTTTGTGGTCATTATGCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGcc                                                                                                                                                                                                                                                                                   <  1:1300358‑M2/128‑1 (MQ=255)
  gaaatcattgcctgcccggtttgcaacggaaaactttggtataaccaggaaaaacaagagcttatttgcaaactggacaacctcgctttcccgctgcgtgatggcattccagtgttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGa                                                                                                                                                                                                                                                                                 <  1:1205646‑M2/130‑1 (MQ=255)
  gaaatcattgcctgcccggtttgcaacggaaaactttggtataaccaggaaaaacaagagcttatttgcaaactggacaacctcgctttcccgctgcgtgatggcattccagtgttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGCGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGa                                                                                                                                                                                                                                                                                 >  2:210323‑M2/160‑289 (MQ=255)
     atcattgcctgcccggtttgcaacggaaaactttggtataaccaggaaaaacaagagcttatttgcaaactggacaacctcgctttcccgctgcgtgatggcattccagtgttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAgc                                                                                                                                                                                                                                                                               >  1:959739‑M2/157‑288 (MQ=255)
       cattgcctgcccggtttgcaacggaaaactttggtataaccaggaaaaacaagagcttatttgcaaactggacaacctcgctttcccgctgcgtgatggcattccagtgttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGca                                                                                                                                                                                                                                                                            <  1:1441799‑M2/135‑1 (MQ=255)
           gcctgcccggtttgcaacggaaaactttggtataaccaggaaaaacaagagcttatttgcaaactggacaacctcgctttcccgctgcgtgatggcattccagtgttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGcatcat                                                                                                                                                                                                                                                                        <  1:1080478‑M2/139‑1 (MQ=255)
                cccggtttgcaacggaaaactttggtataaccaggaaaaacaagagcttatttgcaaactggacaacctcgctttcccgctgcgtgatggcattccagtgttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTg                                                                                                                                                                                                                                                                    >  1:1778916‑M2/146‑288 (MQ=255)
                     tttgcaacggaaaactttggtataaccaggaaaaacaagagcttatttgcaaactggacaacctcgctttcccgctgcgtgatggcattccagtgttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAAcc                                                                                                                                                                                                                                                              <  2:948010‑M2/149‑1 (MQ=255)
                        gcaaaggaaaactttgttataaccgggaaaaacaagagcttatttgcaaactggacaacctcgctttcccgctgcgtgatggcattccagtgttgctggaaacggaagcccgcgtgctgactgctaatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGAt                                                                                                                                                                                                                                                           <  2:855398‑M2/152‑1 (MQ=255)
                                          ataaccaggaaaaacaagagcttatttgcaaactggacaacctcgctttcccgctgcgtgatggcattccagtgttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCcg                                                                                                                                                                                                                                          <  1:1013908‑M2/169‑1 (MQ=255)
                                          ataaccaggaaaaacaagagcttatttgcaaactggacaacctcgctttcccgctgcgtgatggcattccagtgttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCcg                                                                                                                                                                                                                                          <  2:105883‑M2/169‑1 (MQ=255)
                                          ataaccaggaaaaacaagagcttatttgcaaactggacaacctcgctttcccgctgcgtgatggcattccagtgttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCcg                                                                                                                                                                                                                                          <  1:1799205‑M2/169‑1 (MQ=255)
                                           taaccaggaaaaacaagagcttatttgcaaactggacaacctcgctttcccgctgcgtgatggcattccagtgttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCcgcg                                                                                                                                                                                                                                        <  1:1495732‑M2/171‑1 (MQ=255)
                                              ccaggaaaaacaagagcttatttgcaaactggacaacctcgctttcccgctgcgtgatggcattccagtgttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCg                                                                                                                                                                                                                                     >  1:249785‑M2/116‑289 (MQ=255)
                                                        caagagcttatttgcaaactggacaacctcgctttcccgctgcgtgatggcattccagtgttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGc                                                                                                                                                                                                                           <  1:933397‑M2/184‑1 (MQ=255)
                                                        caagagcttatttgcaaactggacaacctcgctttcccgctgcgtgatggcattccagtgttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGc                                                                                                                                                                                                                           <  1:1515975‑M2/184‑1 (MQ=255)
                                                        caagagcttatttgcaaactggacaacatcgctttcccgctgcgtgatggcattccagtgttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCTACGCGTCTGCCCGGTAAACCATTGGTGGATTTTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCCCGCGGGAATCAGGTGACGAGCGCATTATCGTGGCAACCGAACAGGAGGATGTTGCCAGAGCCGTGGAAGcc                                                                                                                                                                                                                             >  2:1263723‑M2/106‑287 (MQ=255)
                                                             ggtttgttgcaaaatggacaacctccctttccggctgcgggatagcattgcagtgttgctggaaacggaggccggcgtgctgactgctgatgggagtaaaTCATGAGTTTAGTGATCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATTTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGc                                                                                                                                                                                                                       <  2:922647‑M2/188‑1 (MQ=255)
                                                             gcttatttgcaaactggacaacctcgctttcccgctgcgtgatggcattccagtgttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCg                                                                                                                                                                                                                      >  1:346720‑M2/101‑289 (MQ=255)
                                                              cttatttgcaaactggacaacctcgctttcccgctgcgtgatggcattccagtgttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCg                                                                                                                                                                                                                      <  2:157190‑M2/189‑1 (MQ=255)
                                                               ttatttgcaaactggacaacctcgctttcccgctgcgtgatggcattccagtgttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGt                                                                                                                                                                                                                    <  2:1778916‑M2/191‑1 (MQ=255)
                                                               ttatttgcaaactggacaacctcgctttcccgctgcgtgatggcattccagtgttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGt                                                                                                                                                                                                                    <  2:293671‑M2/191‑1 (MQ=255)
                                                                tatttgcaaactggacaacctcgctttcccgctgcgtgatggcattccagtgttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTg                                                                                                                                                                                                                   <  2:336140‑M2/192‑1 (MQ=255)
                                                                 atttgcaaactggacaacctcgctttcccgctgcgtgatggcattccagtgttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGa                                                                                                                                                                                                                  <  1:485335‑M2/193‑1 (MQ=255)
                                                                   ttgcaaactggacaacctcgctttcccgctgcgtgatggcattccagtgttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAg                                                                                                                                                                                                                <  1:1887461‑M2/195‑1 (MQ=255)
                                                                   ttgcaaactggacaacctcgcttgccggctgcgggatggcattccagtgttggtggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGGTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAg                                                                                                                                                                                                                <  1:1358294‑M2/195‑1 (MQ=255)
                                                                                    tcgctttcccgcggcgggatggacttcaagtgttgcgggaaacggaagccagcttgcggcctgctgatgggactaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGcc                                                                                                                                                                                               <  2:1176499‑M2/212‑1 (MQ=255)
                                                                                     cgctttcccgctgcgtgatggcattccagtgttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCg                                                                                                                                                                                              >  1:704811‑M2/77‑289 (MQ=255)
                                                                                     cgctgtcccgctgcgtgatggcattccagtgttgcttgaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACGGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCg                                                                                                                                                                                              <  1:1461945‑M2/213‑1 (MQ=255)
                                                                                          tcccgtggcgtgatggcattccagtggtgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGatcat                                                                                                                                                                                         <  2:1883821‑M2/218‑1 (MQ=255)
                                                                                          tcccgctgcgtgatggcattccagtgttgctgggaacggaagcccccgtgctgactgctgatgggaggaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGAAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGatcat                                                                                                                                                                                         <  2:1214443‑M2/218‑1 (MQ=255)
                                                                                            ccgctgcgtgatggcattccagtgttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGt                                                                                                                                                                                                                    >  1:1129393‑M2/70‑260 (MQ=255)
                                                                                              gctgcgggttggcattccaggtttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCAGTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATcagt                                                                                                                                                                                     <  2:1220590‑M2/222‑2 (MQ=255)
                                                                                                ggcggtatggcattacagttgtgctggaaccggaagcgcgcgtgctgactgctgatgttgtgaaaTCATGAGTTTGGTGGTAATTATTCCCGCGCGCTACGCGTCCACGCGGCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGGGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCAtcagtc                                                                                                                                                                                    <  2:1493270‑M2/223‑3 (MQ=255)
                                                                                                      atggcattccagtgttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTgg                                                                                                                                                                                                                        >  2:1709576‑M2/60‑246 (MQ=255)
                                                                                                            ttccagtgttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCCACCGATCATGAGGATGTTGCCCGCGCCGTTTAAGCCGCCGGCGGGGAAGTATTTATGACgcgcgccgatcatcaggtgtcccttataca                                                                                                                                                                        >  2:349983‑M2/54‑274 (MQ=255)
                                                                                                            ttccagtgttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCg                                                                                                                                                                                              <  1:644575‑M2/213‑1 (MQ=255)
                                                                                                            ttccagtgttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGt                                                                                                                                                                       <  1:1992150‑M2/236‑1 (MQ=255)
                                                                                                                  tgttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGt                                                                                                                                                                                                                    >  1:693937‑M2/48‑238 (MQ=255)
                                                                                                                    ttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAAcc                                                                                                                                                                                                                                                              <  2:230993‑M2/149‑1 (MQ=255)
                                                                                                                    ttgctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAAcc                                                                                                                                                                                                                                                              >  1:230993‑M2/46‑194 (MQ=255)
                                                                                                                      gctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGACGCCGCTGGCGGGGAAGTATGTATGACGCGCGCCGACCATCAGTCAGGCACAGAACGTCTGGCggacg                                                                                                                                                             >  2:1487547‑M2/44‑287 (MQ=255)
                                                                                                                       ctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGt                                                                                                                                                            >  2:1810685‑M2/43‑289 (MQ=255)
                                                                                                                       ctggaaacggaagcccgcgtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGt                                                                                                                                                            >  1:389665‑M2/43‑289 (MQ=255)
                                                                                                                                         gtgctgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCAt                                                                                                                                           <  1:1720412‑M2/264‑1 (MQ=255)
                                                                                                                                             tgactgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAg                                                                                                                                       <  2:704811‑M2/268‑1 (MQ=255)
                                                                                                                                               actgctgatgagagtaaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGcg                                                                                                                                     >  1:13560‑M2/19‑288 (MQ=255)
                                                                                                                                                             taaaTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCg                                                                                                                      >  1:354361‑M2/5‑289 (MQ=255)
                                                                                                                                                                aTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGt                                                                                                                           <  2:492418‑M2/280‑1 (MQ=255)
                                                                                                                                                                aTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGt                                                                                                                           >  1:492418‑M2/2‑281 (MQ=255)
                                                                                                                                                                aTCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTa                                                                                                                   <  1:210323‑M2/288‑1 (MQ=255)
                                                                                                                                                                     gAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACCCGGTCATCGTTAAcgt                                                                                                               >  2:1902781/1‑288 (MQ=255)
                                                                                                                                                                     gAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAAtgtg                                                                                                              <  1:1769140/289‑1 (MQ=255)
                                                                                                                                                                     gAGCTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCACGGTAAACCACTGGCTGATATTAACGGCAAACCCATGATTATTC‑CATTCTTGCACGTGTGCGTGAATCAGGTCCCGAGCGCATCATCGTGGCAACCGCGCATGAGGATGTTGCCCGCCCCGTTCACGCCGCTGGATGTGAAGTATGTACGACGCGCCCCGAACATCAACCAGGAACAGAACATCTGGCGGCGGTTTGTGCAAATTGCGCATTCAGTACCGACCAGGTACTCGTTAAtgtg                                                                                                              >  2:1998569/1‑288 (MQ=255)
                                                                                                                                                                                 cATTATTCCCGCGCTCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTCACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGaa                                                                                                  <  1:1263723/289‑1 (MQ=255)
                                                                                                                                                                                 cATTATTCCCGCCCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGAGGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGaa                                                                                                  <  1:690517/289‑1 (MQ=255)
                                                                                                                                                                                     attCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGAACCga                                                                                              >  1:1654853/1‑289 (MQ=255)
                                                                                                                                                                                           gcgcgcTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGAtcatca                                                                                                                                                                                       <  2:1626199/194‑1 (MQ=255)
                                                                                                                                                                                            cgcgcTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGAACCGATGATcc                                                                                        >  2:767364/1‑288 (MQ=255)
                                                                                                                                                                                            cgcgcTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGAACCGATGATc                                                                                         >  1:983435/1‑287 (MQ=255)
                                                                                                                                                                                            cgcgcTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGAACCGATGATc                                                                                         >  1:835757/1‑287 (MQ=255)
                                                                                                                                                                                            cgcgcTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGAACATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGGGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGGTGTCGAAAAATGAGCATTCAGCGACGACACGGGGATAGTTAATGTGCGGGGTGATGAACCGAGGATcc                                                                                        >  2:690517/1‑288 (MQ=255)
                                                                                                                                                                                              cgcTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGAACCGATGATCCCt                                                                                      >  2:1455403/1‑288 (MQ=255)
                                                                                                                                                                                              cgcTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGAACCGATGATCCCt                                                                                      >  1:1677034/1‑288 (MQ=255)
                                                                                                                                                                                                   cgcgTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGAACCGATGATCCCTGCGACa                                                                                >  1:289085/1‑289 (MQ=255)
                                                                                                                                                                                                         cACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGaca                                                                                                                               >  1:413480/1‑236 (MQ=255)
                                                                                                                                                                                                         cACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGAACCGATGATCCCTGCGACAATCAt                                                                           >  1:594999/1‑288 (MQ=255)
                                                                                                                                                                                                         cACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGAGTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGaca                                                                                                                               <  2:413480/236‑1 (MQ=255)
                                                                                                                                                                                                           cgcgTCCGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCCTCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCCGCGACGACACGGTGACCGTTAATGTGCAGGGTGATGAACCGCTGATCCCTGCGACAATctgtc                                                                         >  2:1186406/1‑284 (MQ=255)
                                                                                                                                                                                                              gTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGAACCGATGATCCCTGCGACAATCATTCGTc                                                                      <  1:767364/288‑1 (MQ=255)
                                                                                                                                                                                                               tCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGcga                                                                                                                                    <  2:1107071/225‑1 (MQ=255)
                                                                                                                                                                                                                 tGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGAACCGATGATCCCTGCGACAATCATTCGTCAGGt                                                                  >  1:853919/1‑289 (MQ=255)
                                                                                                                                                                                                                  gCCCTGTAAACACTTGTTTGCTCTGAACGTCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGTTCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGAACCGATGATCCCTGCGACAATCATTCGTCAGGtt                                                                 <  2:471306/289‑1 (MQ=255)
                                                                                                                                                                                                                  gCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGAACCGATGATCCCTGCGACAATCATTCGTCAGGtt                                                                 <  1:1663157/289‑1 (MQ=255)
                                                                                                                                                                                                                        tAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGAACCGATGATCCCTGCGACAATCATTCGTCAGGTTGCTGAt                                                           >  1:158473/1‑289 (MQ=255)
                                                                                                                                                                                                                           aCCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGAACCGATGATCCCTGCGACAATCATTCGTCAGGTTGCTGATaa                                                         >  1:1442648/1‑288 (MQ=255)
                                                                                                                                                                                                                            ccAGTGGTTGGTATGAACGGCAACCACATGATTTGTCATGTTCTTGAGCGCGCGCGTGAATCAGGTGCGGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGAACCGATGATCCCTGCGACAATCATTCGTCAGGTTGCTGATAAcc                                                       <  2:263534/289‑1 (MQ=255)
                                                                                                                                                                                                                                tGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGt                                                                                                                           <  1:527495/217‑1 (MQ=255)
                                                                                                                                                                                                                                tGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGt                                                                                                                           >  2:527495/1‑217 (MQ=255)
                                                                                                                                                                                                                                tGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGAACCGATGATCCCTGCGACAATCATTCGTCAGGTTGCTGATAACCTCGc                                                   <  1:898610/289‑1 (MQ=255)
                                                                                                                                                                                                                                   ttGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGAACCGATGATCCCTGCGACAATCATTCGTCAGGTTGCTGATAACCTCGCTc                                                 <  2:594999/288‑1 (MQ=255)
                                                                                                                                                                                                                                      atatTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGt                                                                                                                           >  1:1320511/1‑211 (MQ=255)
                                                                                                                                                                                                                                      atatTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATAGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACTCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGCAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGGGATGAACCGATGATACATGCGACAATCATTGCTCAGGTTGCTGATAAccacctgtat                                               >  2:529764/1‑279 (MQ=255)
                                                                                                                                                                                                                                       tataagggacagaCCCAGGATTGTTCATGTGCTTGAACGGGGGCGTGAATCAGGGGCGGGGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGAACCGATGATCCCTGCGACAATCATTCGTCAGGTTGCTGATAACCTCGCTCAGCGt                                            <  2:1921487/277‑1 (MQ=255)
                                                                                                                                                                                                                                          tAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGAACCGATGATCCCTGCGACAATCATTCGTCAGGTTGCTGATAACCTCGCTCAGCGTCAg                                         <  2:835757/289‑1 (MQ=255)
                                                                                                                                                                                                                                                        tGATTGTTCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGAACCGATGATCCCTGCGACAATCATTCGTCAGGTTGCTGATAACCTCGCTCAGCGTCAGGTGGGTATGGCg                             >  1:1996780/1‑287 (MQ=255)
                                                                                                                                                                                                                                                              ttCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGAACCGATGATCCCTGCGACAATCATTCGTCAGGTTGCTGATAACCTCGCTCAGCGTCAGGTGGGTa                                  >  1:1921487/1‑276 (MQ=255)
                                                                                                                                                                                                                                                              ttCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGAACCGATGATCCCTGCGACAATCATTCGTCAGGTTGCTGATAACCTCGCTCAGCGTCAGGTGGGTATGGCGACTCTGGc                     >  2:1519649/1‑289 (MQ=255)
                                                                                                                                                                                                                                                              ttCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGAACCGATGATCCCTGCGACAATCATTCGTCAGGTTGCTGATAACCTCGCTCAGCGTCAGGTGGGTATGGCGACTCTGGc                     >  2:137512/1‑289 (MQ=255)
                                                                                                                                                                                                                                                              ttCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGAACCGATGATCCCTGCGACAATCATTCGTCAGGTTGCTGATAACCTCGCCCAGCGTCCGGTGGGTATGGCGGCCCTGGc                     >  2:1976376/1‑289 (MQ=255)
                                                                                                                                                                                                                                                              ttCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGAACCGATGATCCCTGCGACAATCATTCGTCAGGTCGCTGATAACCTCGCTCAGCGCCAGGTGGGTATGGCGACTCTGGc                     >  2:64153/1‑289 (MQ=255)
                                                                                                                                                                                                                                                               tCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAACAATGCGCATTCAGCCACGACACGGTGATCGTTAATTTGCACGGTGATGAACCGATGATCCCTGCGACCATCATTCGTCCGGTTGCTGATAACCTCGCTCAGCCGCAGGTGTGTATGTCGACTCTGGCg                    >  2:1849652/1‑289 (MQ=255)
                                                                                                                                                                                                                                                               tCATGTTCTTGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCCGGGTGATGAACCGATGCTCCCTGCGACAACCATGCGTCCGGTTGCTGCTAACCTCGTCCAGTCTCAGGTGGGTATGGCTACTCTTGCg                    >  2:1324535/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                        tGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAAtgt                                                                                                               <  1:1935985/189‑1 (MQ=255)
                                                                                                                                                                                                                                                                        tGAACGCGCGCGTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAAtgt                                                                                                               >  2:1935985/1‑189 (MQ=255)
                                                                                                                                                                                                                                                                                cgcgTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGAACCGATGATCCCTGCGACAATCATTCGTCAGGTTGCTGATAACCTCGCTCAGCGTCAGGTGGGTATGGCGACTCTGGc                     >  2:1460625/1‑271 (MQ=255)
                                                                                                                                                                                                                                                                                cgcgTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGAACCGATGATCCCTGCGACAATCATTCGTCAGGTTGCTGATAACCTCGCTCAGCGTCAGGTGGGTATGGCGACTCTGGCGGTGCCAATCCACAATGc   >  2:627221/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                cgcgTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGAACCGATGATCCCTGCGACAATCATTCGTCAGGTTGCTGATAACCTCGCTCAGCGTCAGGTGGGTATGGCGACTCTGGCGGGGCCAATcccaactgc   >  2:1981536/1‑282 (MQ=255)
                                                                                                                                                                                                                                                                                 gcgTGAATCAGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGTCAGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGAACCGATGATCCCTGCGACAATCATTCGTCAGGTTGCTGATAACCTCGCTCAGCGTCAGGTGGGTATGGCGACTCTGGCGGTGCCAATCCACAATGCg  >  1:1112962/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                                        |||                                                                                                                                                                                                                                                                                       
GGAAAACTTTGGTATAACCAGGAAAAACAAGAGCTTATTTGCAAACTGGACAACCTCGCTTTCCCGCTGCGTGATGGCATTCCAGTGTTGCTGGAAACGGAAGCCCGCGTGCTGACTGCTGATGAGAGTAAAAGTTGATGTAAACTGCTGATGAGAGTAAATCATGAGTTTTGTGGTCATTATTCCCGCGCGCTACGCGTCCACGCGTCTGCCCGGTAAACCATTGGTTGATATTAACGGCAAACCCATGATTGTTCATGTTCTTGAACGCGCGCGTGAAAGTGGTGCCGAGCGCATCATCGTGGCAACCGATCATGAGGATGTTGCCCGCGCCGTTGAAGCCGCTGGCGGTGAAGTATGTATGACGCGCGCCGATCATCAGAGTGGAACAGAACGTCTGGCGGAAGTTGTCGAAAAATGCGCATTCAGCGACGACACGGTGATCGTTAATGTGCAGGGTGATGAACCGATGATCCCTGCGACAATCATTCGTCAGGTTGCTGATAACCTCGCTCAGCGTCAGGTGGGTATGGCGACTCTGGCGGTGCCAATCCACAATGCG  >  CP042583/833612‑834173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A