Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F210 I1 R1 4928 340.1 5480506 96.9% 5310610 252.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 571,601 3 bp→TGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583571,6010AT99.7% 805.6 / ‑5.4 335intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (161/173);  minor base . (1/0);  total (162/173)
Fisher's exact test for biased strand distribution p-value = 4.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583571,6020CG98.8% 779.2 / ‑4.1 335intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (158/172);  minor base T (4/0);  total (162/173)
Fisher's exact test for biased strand distribution p-value = 5.43e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01
*CP042583571,6030TA99.4% 793.2 / NA 335intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base A (161/171);  total (162/173)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 335 total aligned reads displayed.

TCTGGAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATACTCAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGACTCAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATACTACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATC  >  CP042583/571324‑571882
                                                                                                                                                                                                                                                                                      |||                                                                                                                                                                                                                                                                                        
   ggAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCtt                                                                                                                                                                                                                                                                             <  1:2162131/289‑1 (MQ=255)
    gAACTGTTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATTCCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTG‑GTCTGGTTTTGATATCGAATACACCAAAAATTTAACAC‑ATAACCtt                                                                                                                                                                                                                                                                             >  2:296866/1‑288 (MQ=255)
         gggaaCGATCGCCATATTCGGCGCGCAAGTCCTGGTGGTTGTAGATTTCACCGTTTACCGACAGTACGTGGGTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGt                                                                                                                                                                                                                                                                       <  2:1247744/286‑1 (MQ=255)
             aCGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAAc                                                                                                                                                                                                                                                                   <  1:780507/289‑1 (MQ=255)
             aCGATCGCCATATTCGGAGCGCATTGTCTGGTGGTTGTAAATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAAc                                                                                                                                                                                                                                                                   <  2:77156/289‑1 (MQ=255)
                aTCGCCATATTCGGCGCGCAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTg                                                                                                                                                                                                                                                                >  1:928138/1‑289 (MQ=255)
                 tCGCCATATTCGGCGCGCAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCCAGATGACGCATCAGGCGTGACAGCTCGCGGGCTTGCTTACGCAGCTCAACTGCGTCT‑GTATCGCTATCGAATACGCCAAAAATTTAACAA‑ATACACTTCTACGTTAACCTgt                                                                                                                                                                                                                                                               >  2:2738403/1‑288 (MQ=255)
                      atatTCGGCGCGCAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTAttt                                                                                                                                                                                                                                                          <  1:1007678/289‑1 (MQ=255)
                            ggCGCGCAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCt                                                                                                                                                                                                                                                    >  2:1155813/1‑289 (MQ=255)
                              cgcgcAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAAc                                                                                                                                                                                                                                                                   <  1:2607538/272‑1 (MQ=255)
                                   aaTGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTtgtg                                                                                                                                                                                                                                             >  1:402716/1‑289 (MQ=255)
                                   aaTGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGAGGGTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGACTGGCGTTATCGCTGGCATAAATAACGGACCAGTCCGGGCCACGATGAAGCATCAGGCGTGACAGCACGAGGGCTCTCTTACGCAGCCCAACTGCGTCT‑GATGTGATATCGAATCCGACAAATATTTAACAC‑ATAACCTTCCACGCTCACGTGGTTGTTGGTGGTtgttgtt                                                                                                                                                                                                                                             >  2:2204934/1‑288 (MQ=255)
                                          ggtggtTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAAT‑TTAAACAATAACCTTCCCCGTTAACCTGGTATTTGTTCCTTGTTTGTTTTGCtt                                                                                                                                                                                                                                      >  2:1477359/1‑289 (MQ=255)
                                             ggtTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTTTTTGCTTGtt                                                                                                                                                                                                                                   >  1:1950771/1‑289 (MQ=255)
                                               ttGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCt                                                                                                                                                                                                                                                                 >  1:367084/1‑257 (MQ=255)
                                                 gTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTaaa                                                                                                                                                                                                                               >  1:2206655/1‑289 (MQ=255)
                                                 gTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTaaa                                                                                                                                                                                                                               >  1:1931388/1‑289 (MQ=255)
                                                         cACCGTTTACCGCCAGTACGTGGGTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTTGTATTTGCTGCTTTTTGTGTTTGCCTTTTTAAAAAAAttc                                                                                                                                                                                                                        >  2:1695927/1‑286 (MQ=255)
                                                         cACCGTTTACCGCCAGTACGTGGGTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTTTGTTTGCTTGTTTAAAAAAATGcc                                                                                                                                                                                                                       >  1:429848/1‑289 (MQ=255)
                                                         cACCGTTTACCGCCAGTACGTGGGTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGcc                                                                                                                                                                                                                       >  1:561605/1‑289 (MQ=255)
                                                         cACCGTTTACCGCCAGTACGTGGGTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGcc                                                                                                                                                                                                                       >  1:141265/1‑289 (MQ=255)
                                                          ccgcgttACCGCCAGGACGGTGGTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAAGGGCGTGATCGCTGGCATAAATGCCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCg                                                                                                                                                                                                                      <  2:330712/284‑1 (MQ=255)
                                                          cccttttACCGCCATGCCGTGGGTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATTGCCAGCCGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCg                                                                                                                                                                                                                      <  2:134809/285‑1 (MQ=255)
                                                          cccGTTTACGGCCAGTACGTGGGTTTTTTGTTGGTTGTAGGGGGGTGGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCg                                                                                                                                                                                                                      <  2:2590575/288‑1 (MQ=255)
                                                          aCCGTTTACCGCCAGTACGTGGGTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCg                                                                                                                                                                                                                      <  1:1048900/289‑1 (MQ=255)
                                                          aCCGTTTACCGCCAGGACGTGGGTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCg                                                                                                                                                                                                                      <  1:2064092/289‑1 (MQ=255)
                                                                tACCGCCAGTACGTGGGTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAagc                                                                                                                                                                                                                >  2:1579954/1‑289 (MQ=255)
                                                                  ccGCCATTACGTTGTTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTATCCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAagcag                                                                                                                                                                                                              <  1:2240740/289‑1 (MQ=255)
                                                                    cccAGTACGTGGGTTTTTTAGTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAAGTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAgcagca                                                                                                                                                                                                            <  2:1299776/288‑1 (MQ=255)
                                                                            gTGGGTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTgcgc                                                                                                                                                                                                    <  1:463093/289‑1 (MQ=255)
                                                                             tGGGTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCTTTAACCTGGTATTTGTTGTTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCa                                                                                                                                                                                                   >  2:2306014/1‑289 (MQ=255)
                                                                             tGGGTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAAc                                                                                                                                                                                                                                                                   <  2:384105/225‑1 (MQ=255)
                                                                             tGGGTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAAc                                                                                                                                                                                                                                                                   >  1:384105/1‑225 (MQ=255)
                                                                                               tAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCtt                                                                                                                                                                                                                                                                             <  1:2735629/197‑1 (MQ=255)
                                                                                                      gTTGCGCCGCCGCGTTAACGGCAACAGTTAACAGACGTTCGTAGGCGACAATGGCGTTCTCGCTGTCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCGATCTTACGCCGCTCAACAGCGTCT‑GTTTTCATATCGAATACGCCAAAAATTGATCAC‑ATCACCTTCTCCGTTAACCTGATATTTGTTGCTTGTTGTGTTTGCTTGTGTAAAAAAATCCAGCAAAGCAGTACTGTGCGCAAGCGATTTGTCTGTGTACAAATaaa                                                                                                                                                                          >  2:1630116/1‑289 (MQ=255)
                                                                                                      gTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAAc                                                                                                                                                                                                                                                                   >  2:255201/1‑200 (MQ=255)
                                                                                                      gTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATaaa                                                                                                                                                                          >  1:156424/1‑289 (MQ=255)
                                                                                                      gTTGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCAAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTAttt                                                                                                                                                                                                                                                          >  2:246010/1‑209 (MQ=255)
                                                                                                       ttGCGCCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATaaa                                                                                                                                                                          <  1:1489607/288‑1 (MQ=255)
                                                                                                       ttGCGCCCCCGCGTGAACGTCAACAATTGACGGACGTTCGTGGGCGGGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATaaa                                                                                                                                                                          <  2:1931388/288‑1 (MQ=255)
                                                                                                         gcgcCCCCGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCg                                                                                                                                                                                                                      <  1:644/242‑1 (MQ=255)
                                                                                                             ccccGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCCGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTa                                                                                                                                                                   <  1:2721153/289‑1 (MQ=255)
                                                                                                             ccccGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTa                                                                                                                                                                   <  2:634912/289‑1 (MQ=255)
                                                                                                              cccGCGTTAACGTCAACAATTGACAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGc                                                                                                                                                                                                                                        <  1:631687/219‑1 (MQ=255)
                                                                                                                                 ttgacAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTg                                                                                                                                               >  1:2441925/5‑289 (MQ=255)
                                                                                                                                      aGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAgcagca                                                                                                                                                                                                            >  1:2317285/1‑223 (MQ=255)
                                                                                                                                      aGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGaaaaa                                                                                                                                          >  1:2295655/1‑289 (MQ=255)
                                                                                                                                        aCGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTTGATGGGGGAAAAAAg                                                                                                                                        >  2:1445127/1‑289 (MQ=255)
                                                                                                                                         cGTTCGTGGGCGAGAATGGCGTTATCGATGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGaaaaa                                                                                                                                          <  1:1695927/286‑1 (MQ=255)
                                                                                                                                          gTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGt                                                                                                                                      <  1:883252/289‑1 (MQ=255)
                                                                                                                                           ttCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAgg                                                                                                                                       >  2:2009983/1‑287 (MQ=255)
                                                                                                                                            tagggggCGAGAATGGCGTGATCGCTGGGAAAAATAACGGGCCAGTCCGGGCCACGATGACGAATCCAGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTc                                                                                                                                     <  2:438107/284‑1 (MQ=255)
                                                                                                                                            gcGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTc                                                                                                                                     <  1:736546/287‑1 (MQ=255)
                                                                                                                                                gggCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGgtg                                                                                                                                >  1:1240193/1‑289 (MQ=255)
                                                                                                                                                    gagaATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCg                                                                                                                                                                                                                      >  1:2681242/1‑199 (MQ=255)
                                                                                                                                                    gagaATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCg                                                                                                                                                                                                                      <  2:2681242/199‑1 (MQ=255)
                                                                                                                                                        aTGGCGTTATCGCTGGCATAAATACCGGGCCATTGCGGGCCACGATGAGGCCTCAGGCGTGACAGCTCTAGGGCTTTTTTACGCAGCTCAACTGCTTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCGTCTCCGTTAACTTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGat                                                                                                                         <  2:173549/288‑1 (MQ=255)
                                                                                                                                                         tGGCGTTATCGTTGGCATGAACACCGGACCCGGCCGGGCCACGATGAAGCATCACGCGTTGCAGCTCGAGTGCTTTCTTACGCATCTCAACTGCGTCT‑GTTTTGATATCGAATAAGCCAAAAATTGAACAC‑ATAACCTTATCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGatat                                                                                                                       <  2:2102927/289‑1 (MQ=255)
                                                                                                                                                            cGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTg                                                                                                                                   >  2:1411459/1‑274 (MQ=255)
                                                                                                                                                                   gCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATtgat                                                                                                             >  2:1521658/1‑289 (MQ=255)
                                                                                                                                                                                 cGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCg                                                                                                                                                                                                                      >  2:2251564/1‑170 (MQ=255)
                                                                                                                                                                                 cGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCg                                                                                                                                                                                                                      <  2:353076/170‑1 (MQ=255)
                                                                                                                                                                                 cGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCg                                                                                                                                                                                                                      <  2:621513/170‑1 (MQ=255)
                                                                                                                                                                                 cGGACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAAtttt                                                                                               >  2:1507820/1‑289 (MQ=255)
                                                                                                                                                                                   gACCAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATaaa                                                                                                                                                                          >  1:2033267/1‑212 (MQ=255)
                                                                                                                                                                                     ccAGTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTAAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGAAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGc                                                                                           <  2:1555081/289‑1 (MQ=255)
                                                                                                                                                                                     ccAGTCCGGGCACCTGTGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGc                                                                                           <  2:928138/289‑1 (MQ=255)
                                                                                                                                                                                        gTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCa                                                                                                                                                                                                                    >  2:546286/1‑165 (MQ=255)
                                                                                                                                                                                        gTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCggg                                                                                        >  1:2565189/1‑289 (MQ=255)
                                                                                                                                                                                        gTCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCggg                                                                                        >  1:1635223/1‑289 (MQ=255)
                                                                                                                                                                                         tCCGGGCCACGATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGt                                                                                       >  1:684338/1‑289 (MQ=255)
                                                                                                                                                                                          ccGGGCCACGGTGAGTCGTCCGGCGTGACAGCTCTAGGGCATTCTTACGACGCTCCACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGTACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAAGGCCTCAAAGAAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGt                                                                                       <  2:2061356/288‑1 (MQ=255)
                                                                                                                                                                                                   gATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑At                                                                                                                                                                                                                                                                                   <  1:2504547/91‑1 (MQ=255)
                                                                                                                                                                                                   gATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑At                                                                                                                                                                                                                                                                                   >  2:2504547/1‑91 (MQ=255)
                                                                                                                                                                                                   gATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATaaa                                                                                                                                                                          <  1:1176503/196‑1 (MQ=255)
                                                                                                                                                                                                   gATGACGCATCAGGCGTGACAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGgtgt                                                                                                                               <  1:332042/239‑1 (MQ=255)
                                                                                                                                                                                                                  ggtacAGCTCGAGGGCTTTCTTCCGCAGCTCAACTGCCTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACgg                                                              <  1:1312310/285‑1 (MQ=255)
                                                                                                                                                                                                                   tgacAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACggg                                                             >  1:1535943/4‑289 (MQ=255)
                                                                                                                                                                                                                              gggCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTg                                                   <  2:391275/288‑1 (MQ=255)
                                                                                                                                                                                                                                   ttCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGat                                                                                                                         <  1:2382554/213‑1 (MQ=255)
                                                                                                                                                                                                                                   ttCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGat                                                                                                                         >  2:2382554/1‑213 (MQ=255)
                                                                                                                                                                                                                                   ttCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACa                                             <  2:1595878/289‑1 (MQ=255)
                                                                                                                                                                                                                                    tCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑AAAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGATTAAAAAAATGCCGCAAAGCCGCACTGTGCGAAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGAATGTCGACTGGTGAAAAAAGGTCTGGGGTGAGGAGATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCAAAATTTCTGACaa                                            >  2:681396/1‑289 (MQ=255)
                                                                                                                                                                                                                                              cTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGt                                                                                                                                      >  2:1673965/1‑189 (MQ=255)
                                                                                                                                                                                                                                              cTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGt                                                                                                                                      <  1:1673965/189‑1 (MQ=255)
                                                                                                                                                                                                                                              cTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGc                                                                                           <  1:2278542/232‑1 (MQ=255)
                                                                                                                                                                                                                                              cTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTg                                                                                <  1:1132817/243‑1 (MQ=255)
                                                                                                                                                                                                                                              cTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACaa                                            <  1:1874739/279‑1 (MQ=255)
                                                                                                                                                                                                                                              cTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACaa                                            >  2:1874739/1‑279 (MQ=255)
                                                                                                                                                                                                                                              cTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGAttt                                  >  2:1029822/1‑289 (MQ=255)
                                                                                                                                                                                                                                              cTCAACTGCGACT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAAACTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAACTGCCGCAAAGCAGCACTGTGCGCCAGCGATTTCGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGATTGTTTATATTTTACGGGGGCCAAATTGCTGACAAATTTCGAttt                                  >  2:2044734/1‑289 (MQ=255)
                                                                                                                                                                                                                                                        tCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAg                                                                                                                                                                                                 <  1:2094474/120‑1 (MQ=255)
                                                                                                                                                                                                                                                        tCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATaaa                                                                                                                                                                          >  1:1595508/1‑143 (MQ=255)
                                                                                                                                                                                                                                                        tCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATAtattta                                                                                                                  >  1:1975135/1‑199 (MQ=255)
                                                                                                                                                                                                                                                        tCT‑GTTTTGATATCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATAtattta                                                                                                                  <  2:1975135/199‑1 (MQ=255)
                                                                                                                                                                                                                                                                     tCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTTTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGa            <  1:183447/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                     tCGAATACGCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGa            <  1:1507820/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                             gCCAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTGGTGTTTGCTGGTTTAAAAAAATGCCGCAAATCAGCACTGTGCGCAAGCGATGTGGCGGTGAAAAAATAAAAAACGTAATGGGGATTGTCGATTGGTGAAAAAAGGTCTGGTGGGAGGATATATTTATTGATGAATCGATAAGTGTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCGGACAGAGTGCGATTTGTTCATGCCGGTTGCGGCGTGAACGCTTTa    >  2:864156/1‑289 (MQ=255)
                                                                                                                                                                                                                                                                               cAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTTTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGACAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATCATCTTTAGCGGGTTTTCTTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGAGCCGGCGGGAACGCCtttt   >  2:691810/1‑286 (MQ=255)
                                                                                                                                                                                                                                                                               cAAAAATTGAACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATc  <  1:980025/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                      |||                                                                                                                                                                                                                                                                                        
TCTGGAACTGGTAACGATCGCCATATTCGGCGCGCAATGCCTGGTGGTTGTAGATTTCACCGTTTACCGCCAGTACGTGGGTTTTTTGTTGGTTGTAGAGAGGTTGCGCCCCCGCGTTAACGTCAACAATACTCAGACGTTCGTGGGCGAGAATGGCGTTATCGCTGGCATAAATACCGGACCAGTCCGGGCCACGATGACGCATCAGGCGACTCAGCTCGAGGGCTTTCTTACGCAGCTCAACTGCGTCT‑GTTTTGATATCGAATACGCCAAAAATACTACAC‑ATAACCTTCTCCGTTAACCTGGTATTTGTTGCTTGTTGTGTTTGCTTGTTTAAAAAAATGCCGCAAAGCAGCACTGTGCGCAAGCGATTTGGCGGTGAAAAAATAAAAAACGTAATGGTGATTGTCGATTGGTGAAAAAAGGTCTGGTGTGAGGATATATTTATTGATGAATCGATAATTTTTAGCGGGTTTTATTGAATGTTTATATTTTACGGGGGCCAAATTGCTGACAAAGTGCGATTTGTTCATGCCGGATGCGGCGTGAACGCCTTATC  >  CP042583/571324‑571882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A