Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F210 I1 R1 4928 340.1 5480506 96.9% 5310610 252.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP042583 614,149 3 bp→TGA intergenic (–/–) – / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP042583614,1490AT99.7% 716.9 / NA 303intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (145/157);  total (145/158)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583614,1500CG99.3% 706.9 / NA 303intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base C (0/1);  major base G (144/156);  minor base T (1/1);  total (145/158)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*CP042583614,1510TA98.7% 701.5 / NA 303intergenic (–/–)–/––/–
Reads supporting (aligned to +/- strand):  ref base T (0/3);  major base A (144/151);  minor base G (0/4);  total (145/158)
Fisher's exact test for biased strand distribution p-value = 1.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 303 total aligned reads displayed.

TGGTGTTACTGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGACTCATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTGCTTGCTGCGCTAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCACTTTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGATAGGTGCGGCTTATTCCGCCACTCTGGTCATGGTTATCTTCGCTATCGGTCTTATTAAGCTGGTAATCACTGCGTA  >  CP042583/613872‑614426
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                                   
aggtgtttgagAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGa                                                                                                                                                                                                                                                                             >  1:27928/11‑288 (MQ=255)
   tgtttgagAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTTGTAAATGACGCGGCCTGATTGACTGATCg                                                                                                                                                                                                                                                                          >  1:300885/8‑288 (MQ=255)
   tgtttgagAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCg                                                                                                                                                                                                                                                                          >  2:1720156/8‑288 (MQ=255)
   tgtttgagAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATAGAGGTAATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACCCGGCTTGATTCACTGATCGt                                                                                                                                                                                                                                                                         >  2:1670328/8‑289 (MQ=255)
      ttgagAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCa                                                                                                                                                                                                                                                                       >  2:1128005/5‑288 (MQ=255)
        gagAGCATCCCCCTGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGTTGTGACATGGAAGGCCTTTGCTGACCCGCGGCCAATTTATAGCGAACCTGTGCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGGCTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCg                                                                                                                                                                                                                                                                    <  2:1301335/287‑1 (MQ=255)
        gagAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCg                                                                                                                                                                                                                                                                    <  1:77590/287‑1 (MQ=255)
        gagAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCg                                                                                                                                                                                                                                                                    <  1:2211199/287‑1 (MQ=255)
              aTCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCa                                                                                                                                                                                                                                                               <  2:27928/288‑1 (MQ=255)
              aTCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCa                                                                                                                                                                                                                                                               <  1:1943061/288‑1 (MQ=255)
               tCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCATTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCAtt                                                                                                                                                                                                                                                             >  2:314111/1‑289 (MQ=255)
                  ccccGGAAACTTAATGGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATTGAAGGCCGTGGCTGACCCGAGGCCAATTTATCGCGACCCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGt                                                                                                                                                                                                                                                          <  2:13866/289‑1 (MQ=255)
                  cccAGGAAACTTCATTGGTAACAAAGGGTTCAGTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGTCTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGt                                                                                                                                                                                                                                                          <  2:1222233/289‑1 (MQ=255)
                  cccAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGt                                                                                                                                                                                                                                                          <  1:1540588/289‑1 (MQ=255)
                  cccAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGt                                                                                                                                                                                                                                                          <  1:236033/289‑1 (MQ=255)
                  cccAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGt                                                                                                                                                                                                                                                          <  1:1215794/289‑1 (MQ=255)
                  cccAGGAAACTTCATTGCTAAAAAAGGTTGCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGt                                                                                                                                                                                                                                                          <  1:143653/289‑1 (MQ=255)
                          aCTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCaa                                                                                                                                                                                                                                                  <  2:2034930/289‑1 (MQ=255)
                              cATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATAcc                                                                                                                                                                                                                                              >  1:1723670/1‑289 (MQ=255)
                                 tGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCt                                                                                                                                                                                                                                           <  2:1948746/289‑1 (MQ=255)
                                 tGCTAAAAAAGGTTTCATTTTCAGTTTGATGTGACATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCt                                                                                                                                                                                                                                           >  2:1589592/1‑289 (MQ=255)
                                                                          ggCCGTGGCTGCCCCGCGGCCAATGTATAGCGATCCTGTCCCTGGCGAGTCATGAATGGCGTCGATGCTGCCGAACCCTGGAACTTGTTCCCCCGACCAGCTTATTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCGATAATAAGCGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACAtt                                                                                                                                                                                                   <  2:2308907/288‑1 (MQ=255)
                                                                            ccGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACg                                                                                                                                                                                                                      >  1:1696820/1‑267 (MQ=255)
                                                                             cGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTTGGGCGTAGCCACGCAGCTCCCACGGGAAATTTCGa                                                                                                                                                                                                >  2:69867/1‑288 (MQ=255)
                                                                               tGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGa                                                                                                                                                                                                                                                                             <  1:1989222/209‑1 (MQ=255)
                                                                               tGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATTGTCACCGTGGCAGTGTTCTGTGCAATACCGCTAATTTGTGGGGCGTAGGCACGCAGCTCCCACGGTAACATTCGATCg                                                                                                                                                                                             >  1:538275/1‑289 (MQ=255)
                                                                                     aCCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTc                                                                                                                                                                                       >  1:110732/1‑289 (MQ=255)
                                                                                       ccGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGc                                                                                                                                                                                     <  1:1494953/289‑1 (MQ=255)
                                                                                          cgGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAg                                                                                                                                                                                  >  2:1771119/1‑289 (MQ=255)
                                                                                          cgGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAg                                                                                                                                                                                  >  2:785202/1‑289 (MQ=255)
                                                                                            gCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCCCGCATCTCCCACGGTAACATTCGATAGTCAcccccccgt                                                                                                                                                                                 >  2:421535/1‑280 (MQ=255)
                                                                                                aTTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCcgc                                                                                                                                                                            <  1:1670328/289‑1 (MQ=255)
                                                                                                 tttATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCcgcg                                                                                                                                                                           <  1:444994/289‑1 (MQ=255)
                                                                                                  ttATGGCGCACCTGTCCCTGTGGATTCAGGAAGGGGGTCGATGCTGCCGAAGCCTGGAAATTGTTCACCCGACCATCTTCTTCCGTGACTTTGGCATCCAGCGTGCCGTGAACAGACTGATTGAGGTCATCAATAATGAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCgcgc                                                                                                                                                                          <  2:2466835/289‑1 (MQ=255)
                                                                                                  ttATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCgcgc                                                                                                                                                                          <  1:374851/289‑1 (MQ=255)
                                                                                                  ttATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCgcgc                                                                                                                                                                          <  1:909963/289‑1 (MQ=255)
                                                                                                  tactacggAACCGGTCCCTGCCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCgcgc                                                                                                                                                                          <  2:735529/282‑1 (MQ=255)
                                                                                                           aCCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCa                                                                                                                                                                                                                                                               <  2:1476648/195‑1 (MQ=255)
                                                                                                           aCCTGTCCCTGGCGAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGc                                                                                                                                                                                                                          <  2:2618108/232‑1 (MQ=255)
                                                                                                                        gAGTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGaa                                                                                                                                                    <  1:104112/289‑1 (MQ=255)
                                                                                                                          gTCAGGAAGGGCGTCGATGCTGCCGAAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTATGTGCAATACCGCTAATTTGTGGGCAGTAGCCACGCAGCCCCCACGGGAACATCCGATCGCTCCTCTCCATCGCCCCGCCGTTATAAGAAAAAcctctgaaaa                                                                                                                                                  >  2:2163974/1‑281 (MQ=255)
                                                                                                                                                           aaaTTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGtt                                                                                                                  <  1:2279751/288‑1 (MQ=255)
                                                                                                                                                           aaaTTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGtt                                                                                                                  <  1:116483/288‑1 (MQ=255)
                                                                                                                                                                         aCCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGAt                                                                                                                                                                                               >  2:2177340/1‑197 (MQ=255)
                                                                                                                                                                           cATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTg                                                                                                 <  1:1761367/289‑1 (MQ=255)
                                                                                                                                                                                tcttcCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACGGAAATCGGTTTCGCCGATGGTTAACTTAGCGCCTAATTGCggga                                                                                            >  2:1330602/1‑287 (MQ=255)
                                                                                                                                                                                    ccGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAg                                                                                                                                                                                                                   <  1:2537362/166‑1 (MQ=255)
                                                                                                                                                                                    ccGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATgg                                                                                        >  2:199094/1‑289 (MQ=255)
                                                                                                                                                                                    ccGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCAACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTaa                                                                                                                >  1:505835/1‑265 (MQ=255)
                                                                                                                                                                                       tCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGt                                                                                                                                                                                                                                                          <  1:1332184/124‑1 (MQ=255)
                                                                                                                                                                                        cACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACg                                                                                     <  1:2562447/288‑1 (MQ=255)
                                                                                                                                                                                            ttGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGACATCGGTTTCGCCGCGGGTTAACTTAGACCCTAATTTCGGTAATTGGCaaaaaa                                                                                >  2:1823011/1‑289 (MQ=255)
                                                                                                                                                                                                cATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGAt                                                                             <  1:2075661/288‑1 (MQ=255)
                                                                                                                                                                                                       cGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGta                                                                                                                                                                     >  1:1855305/1‑193 (MQ=255)
                                                                                                                                                                                                       cGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGaa                                                                                   >  1:2461918/1‑275 (MQ=255)
                                                                                                                                                                                                        gTGCCCTGAACAGACTGATTGAGGGCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGATAGGTGCgc                                                                     <  1:894738/288‑2 (MQ=255)
                                                                                                                                                                                                            ccTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGTTTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGATAGGTGCgcgata                                                                 <  2:901432/288‑6 (MQ=255)
                                                                                                                                                                                                                aaCAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGt                                                                                                                                                                                                                                                          >  1:139913/1‑99 (MQ=255)
                                                                                                                                                                                                                aaCAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGaa                                                                                                                                                    <  1:522908/201‑1 (MQ=255)
                                                                                                                                                                                                                     aCTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGAt                                                                                                                                                                                               <  2:2575422/153‑1 (MQ=255)
                                                                                                                                                                                                                         aTTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCa                                                                                                                                                                                                                                                               >  1:2448440/1‑85 (MQ=255)
                                                                                                                                                                                                                         aTTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCa                                                                                                                                                                                                                                                               <  2:2448440/85‑1 (MQ=255)
                                                                                                                                                                                                                          ttGAGGTCATCAATAATAACCGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGATAGGTGCGCGATATTCCGcctgactggt                                                  <  1:745771/289‑9 (MQ=255)
                                                                                                                                                                                                                          ttGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCgcgc                                                                                                                                                                          >  1:1630123/1‑169 (MQ=255)
                                                                                                                                                                                                                          ttGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTa                                                                                                            <  2:1048222/231‑1 (MQ=255)
                                                                                                                                                                                                                          ttGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGATAGGTGCGCGATATTCCGcctgactggt                                                  <  1:2604934/289‑9 (MQ=255)
                                                                                                                                                                                                                          ttGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGATAGGTGCGCGATATTCCGcctgactgg                                                   >  2:2492956/1‑281 (MQ=255)
                                                                                                                                                                                                                           tGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGCTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGATAGGTGCGCGATATTCCGCCTGACTGGTc                                                 <  1:2168567/289‑1 (MQ=255)
                                                                                                                                                                                                                               gTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCg                                                                                                                        <  2:2263666/214‑1 (MQ=255)
                                                                                                                                                                                                                               gTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGATAGGTGCGCGATATTCCGCCTGACTGGTCATgg                                             >  1:1994957/1‑289 (MQ=255)
                                                                                                                                                                                                                                   tcaATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATgg                                                                                        <  2:2704170/242‑1 (MQ=255)
                                                                                                                                                                                                                                   tcaATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATgg                                                                                        >  1:2704170/1‑242 (MQ=255)
                                                                                                                                                                                                                                   tcaATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGATAGGt                                                                         >  1:865049/1‑257 (MQ=255)
                                                                                                                                                                                                                                      ctattcaaCGGGCCTGGCGGGGCTTGTTTCTGGTCAATGACTGGGCCACGTTGACTGATACGCACGGTGGCATTGGTCTGTGCACTCCCGCTAATTTATGGGCCAGCGCCAGGCAGCTCCCACGGTAACAGTCGATCGTCAATCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGATGTGAAATCGGGTGCGCGGAGGGTTAACTTAGGGCCTAATTGCGGTAATGGACGAAAGAGATAGGTGCGCGATATTCCGCCTGACTGGTCATGGTTATCtt                                      <  2:2391719/283‑1 (MQ=255)
                                                                                                                                                                                                                                          taaACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCg                                                                                                                        >  2:933855/1‑203 (MQ=255)
                                                                                                                                                                                                                                          taaACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGATAGGTGCGCGATATTCCGCCTGACTGGTCATGGTTATCTTCGCt                                  >  1:1714998/1‑289 (MQ=255)
                                                                                                                                                                                                                                            cacgggacgtgcctgACTTTTTTCTGTTAAATGCCGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGAGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGATAGGTGCGCGATATTCCGCCTGACTGGTCATGGTTATCTTCGCTAt                                <  2:884739/275‑1 (MQ=255)
                                                                                                                                                                                                                                            aaCGGGCCTGGCTGAACTTTTTTCCGGTAAATGCCCCTGCCTGTTTGACTGATCGTCACGTGGGCATTGGTCTGGGCAATTCCGCTAATTTGTGGGGCGTAGCCCCGCAGCTCCCACGGTAAAATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGATAGGTGCGCGATATTCCGCCTGACTGGTCATGGTTATCTTCGCTAt                                <  2:538275/289‑1 (MQ=255)
                                                                                                                                                                                                                                            aaCGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTTGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGATAGGTGCGCGATATTCCGCCTGACTGGTCATGGTTATCTTCGCTAt                                <  2:828847/289‑1 (MQ=255)
                                                                                                                                                                                                                                            aaCGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGATAGGTGCGCGATATTCCGCCTGACTGGTCATGGTTATCTTCGCTAt                                <  1:626889/289‑1 (MQ=255)
                                                                                                                                                                                                                                             aCGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCgg                                                                                                                                >  1:1602852/1‑192 (MQ=255)
                                                                                                                                                                                                                                                gtccTGGCGGGACTTTTTTCTGGTAAAGGACGCGGCCTGTTTGACTGATCGTCACCGTGGCATTGGTCTTTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATAGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGATAGGTGCGCGATATTCCGCCTGACTGGTCATGGTTATCTTCGCTATCgg                             <  2:514507/286‑1 (MQ=255)
                                                                                                                                                                                                                                                 gCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGaaa                                                                                                                                                              <  2:1268808/158‑1 (MQ=255)
                                                                                                                                                                                                                                                 gCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGaaa                                                                                                                                                              >  1:1268808/1‑158 (MQ=255)
                                                                                                                                                                                                                                                 gCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCg                                                                                                                        <  1:2529524/196‑1 (MQ=255)
                                                                                                                                                                                                                                                 gCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCg                                                                                                                        >  2:2529524/1‑196 (MQ=255)
                                                                                                                                                                                                                                                 gCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAACAAGATAGGTGCGCGATATTCCGCCTTACCGGTCATGGTTATCTTCGCTAACgg                             >  2:1440113/1‑287 (MQ=255)
                                                                                                                                                                                                                                                 gCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGCTAGGTGCGCGATATTCCGCCTGACTGGTCATGGTTATCttgcctagtgg                             >  2:626889/1‑278 (MQ=255)
                                                                                                                                                                                                                                                 gCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGATAGGTGCGCGATATTCCGCCTGACTGGTCATGGTTATCTTCGCTAt                                <  2:1639616/284‑1 (MQ=255)
                                                                                                                                                                                                                                                 gCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGATAGGTGCGCGATATTCCGCCTGACTGGTCATGGTTATCTTCGCTAt                                >  1:1639616/1‑284 (MQ=255)
                                                                                                                                                                                                                                                 gCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGATAGGTGCGCGATATTCCGCCTGACTGGTCATGGTTATCTTCGCTATCgg                             >  2:40555/1‑287 (MQ=255)
                                                                                                                                                                                                                                                  ccTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGaa                                                                                                                                                    >  2:1186955/1‑167 (MQ=255)
                                                                                                                                                                                                                                                        gggACTTTTTTCTGGTAAAGTACTGCGCCTTATTGACTGATCCTCACCGTGGCATTGGGCTGTGCAATACCGATAATTTGGGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGATAGGTGCGCGATATTCCGCCTGACTGGTCATGGTTATCTTCGCTATCGGTCttatta                     <  2:1882524/288‑1 (MQ=255)
                                                                                                                                                                                                                                                               ttttCTGGTAAATGCGGGGGGCTGGTTGACTGGTCGTCACCGTGGCATTGGTCTGTGCAGTACCGCTAATTTGTGGGGCGTAGCCACGCAGCTACCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAGTCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGTACGAAAAAGATAGGTGCGCGATATTCCGCCTGACTGGTCATGGTTATCTTCGCTATCGGTCTTATTAAGCTGGTa             <  2:2648285/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                 ttCTGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGATAGGTGCGCGATATTCCGCCTGACTGGTCATGGTTATCTTCGCTATCGGTCTTATTAAGCTGGTaa            >  1:2654482/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                    tGGTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGATAGGTGCGCGATATTCCGCCTGACTGGTCATGGTTATCTTCGCTATCGGTCTTATTAAGCTGGTAATCAc        <  1:1311980/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                     ggTAAATGACGCGGCCTGATTGACTGCTCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGATAGGTGCGCGATATTCCGCCTGACTGGTCATGGTTATCTTCGCTATCGGTCTTATTAAGCTGGTAATCACt       <  1:2492956/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                      gTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGATAGGTGCGCGATATTCCGCCTGACTGGTCATGGTTATCTTCGCTATCGGTCTTATTAAGCTGGTAATCACt       >  1:685185/1‑288 (MQ=255)
                                                                                                                                                                                                                                                                      gTAAATGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTACCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGCTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGATCGGTGCGCGATATTCCGCCTGACTGGTCAGTGTTATCTTCGCTATGGGTCttattactgtcttttataca       >  2:2559228/1‑274 (MQ=255)
                                                                                                                                                                                                                                                                          aTGACGCGGCCTGATTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTACCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGATAGGTGCGCGATATTCCGCCTGACTGGTCATGGTTATCTTCGCTATCGGTCTTATTAAGCTGGTAATCACTGCGTa  <  2:1970360/289‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     |||                                                                                                                                                                                                                                                                                   
TGGTGTTACTGAGCATCCCCCAGGAAACTTCATTGCTAAAAAAGGTTTCATTTTCAGTTTGATGACTCATGGAAGGCCGTGGCTGACCCGCGGCCAATTTATAGCGAACCTGTCCCTGGCGAGTCAGGAAGGGCGTGCTTGCTGCGCTAACCTGGAAATTGTTCACCCGACCATCTTCTTCCGTCACTTTGACATCCAGCGTGCCCTGAACAGACTGATTGAGGTCATCAATAATAAACGGGCCTGGCGGGACTTTTTTCTGGTAAATGACGCGGCCACTTTGACTGATCGTCACCGTGGCATTGGTCTGTGCAATACCGCTAATTTGTGGGGCGTAGCCACGCAGCTCCCACGGTAACATTCGATCGTCACTCGCCAGTGCCGCGCCGGTATAAGAAAAACCATCGAAAATATTGGAACTGAAATCGGTTTCGCCGAGGGTTAACTTAGAGCCTAATTGCGGTAATGGACGAAAAAGATAGGTGCGGCTTATTCCGCCACTCTGGTCATGGTTATCTTCGCTATCGGTCTTATTAAGCTGGTAATCACTGCGTA  >  CP042583/613872‑614426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A